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The map label for this gene is 148661566

Identifier: 148661566

GI number: 148661566

Start: 2002838

End: 2004346

Strand: Direct

Name: 148661566

Synonym: MRA_1774

Alternate gene names: NA

Gene position: 2002838-2004346 (Clockwise)

Preceding gene: 148661565

Following gene: 148661569

Centisome position: 45.31

GC content: 64.88

Gene sequence:

>1509_bases
ATGCCGAGGGGCTGCGCCGGGGCTAGATTCGCGTGCAATGCGTGCCTAAACTTTTTGGCGGGGTTGGGGATTTCTGAACC
GATCAGTCCCGGGTGGGCGGCTATGGAGCGACTAAGCGGACTCGATGCTTTCTTCCTCTATATGGAGACACCGTCGCAGC
CGCTGAACGTGTGCTGCGTCTTGGAGTTGGACACCTCGACGATGCCGGGCGGCTACACGTACGGCCGGTTTCATGCCGCG
TTGGAGAAGTATGTCAAGGCGGCGCCCGAATTTCGGATGAAGCTCGCCGATACCGAGCTTAACCTGGATCACCCCGTGTG
GGTGGACGACGACAATTTTCAGATCCGGCACCACCTGCGCCGGGTCGCTATGCCCGCGCCCGGAGGGCGTCGCGAGCTGG
CCGAGATCTGTGGGTACATCGCCGGGTTGCCGCTGGACCGTGACCGCCCGCTGTGGGAGATGTGGGTCATCGAAGGCGGT
GCCCGTAGCGACACCGTGGCGGTGATGCTCAAGGTCCACCACGCCGTGGTCGACGGTGTCGCCGGTGCGAACCTGCTGTC
CCACCTGTGCAGCCTGCAGCCCGATGCGCCGGCACCGCAACCTGTCCGGGGCACCGGTGGCGGCAATGTGCTGCAGATAG
CTGCGAGTGGGCTGGAGGGGTTCGCGTCGCGGCCAGTGCGGCTGGCGACGGTGGTACCGGCGACAGTGCTCACATTGGTG
CGCACATTGCTGCGTGCCCGTGAGGGCCGTACCATGGCCGCCCCGTTTTCGGCCCCACCGACTCCGTTCAACGGCCCCCT
CGGTCGGCTGCGCAACATCGCGTATACACAGCTCGACATGCGCGACGTCAAGCGTGTCAAGGACCGGTTTGGGGTGACCA
TCAACGATGTGGTGGTGGCGTTGTGTGCCGGAGCGCTACGGCGCTTCCTACTCGAGCACGGCGTGCTGCCCGAGGCCCCG
TTGGTGGCCACCGTGCCGGTTTCGGTACACGACAAGTCGGACCGACCCGGGCGCAACCAGGCCACCTGGATGTTCTGTCG
GGTACCGAGCCAGATCAGCGACCCCGCCCAGCGCATCCGCACCATCGCCGCCGGAAACACCGTCGCTAAAGACCACGCCG
CGGCCATCGGCCCCACCCTGCTGCACGACTGGATTCAGTTCGGCGGCTCGACGATGTTCGGAGCGGCCATGCGGATCTTG
CCGCACATTTCGATAACGCATAGCCCCGCCTACAATCTGATCCTGTCGAATGTGCCCGGACCCCAGGCCCAGTTGTACTT
TCTGGGTTGCCGAATGGACTCGATGTTTCCCCTCGGCCCCCTCCTTGGCAACGCGGGCCTCAACATCACCGTCATGTCCC
TCAACGGGGAACTGGGTGTCGGCATTGTCTCCTGCCCCGACCTGCTGCCGGACCTGTGGGGCGTGGCAGACGGGTTTCCC
GAGGCGCTCAAAGAGCTGCTGGAGTGCAGTGATGACCAGCCGGAAGGCAGCAACCACCAGGACTCCTGA

Upstream 100 bases:

>100_bases
TAGCAGCCTGCCGGTGCCACCGGCGTCTGGGCACCCCAGACTTTCGCGCCGCTGTCACCCGTTGCGAAGGCCCCCGCAAT
GGCACGGTCACCGACATGTG

Downstream 100 bases:

>100_bases
GTCGTACGTTCAGAACCGGTAGTCGGTGCCGGTGCCCAGAACTTCGATGGCTGCGTTGATGTTCGGGATCACTGTGGCGC
CGTATCGGCTGACGATCTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1760/MT1809

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPRGCAGARFACNACLNFLAGLGISEPISPGWAAMERLSGLDAFFLYMETPSQPLNVCCVLELDTSTMPGGYTYGRFHAA
LEKYVKAAPEFRMKLADTELNLDHPVWVDDDNFQIRHHLRRVAMPAPGGRRELAEICGYIAGLPLDRDRPLWEMWVIEGG
ARSDTVAVMLKVHHAVVDGVAGANLLSHLCSLQPDAPAPQPVRGTGGGNVLQIAASGLEGFASRPVRLATVVPATVLTLV
RTLLRAREGRTMAAPFSAPPTPFNGPLGRLRNIAYTQLDMRDVKRVKDRFGVTINDVVVALCAGALRRFLLEHGVLPEAP
LVATVPVSVHDKSDRPGRNQATWMFCRVPSQISDPAQRIRTIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMRIL
PHISITHSPAYNLILSNVPGPQAQLYFLGCRMDSMFPLGPLLGNAGLNITVMSLNGELGVGIVSCPDLLPDLWGVADGFP
EALKELLECSDDQPEGSNHQDS

Sequences:

>Translated_502_residues
MPRGCAGARFACNACLNFLAGLGISEPISPGWAAMERLSGLDAFFLYMETPSQPLNVCCVLELDTSTMPGGYTYGRFHAA
LEKYVKAAPEFRMKLADTELNLDHPVWVDDDNFQIRHHLRRVAMPAPGGRRELAEICGYIAGLPLDRDRPLWEMWVIEGG
ARSDTVAVMLKVHHAVVDGVAGANLLSHLCSLQPDAPAPQPVRGTGGGNVLQIAASGLEGFASRPVRLATVVPATVLTLV
RTLLRAREGRTMAAPFSAPPTPFNGPLGRLRNIAYTQLDMRDVKRVKDRFGVTINDVVVALCAGALRRFLLEHGVLPEAP
LVATVPVSVHDKSDRPGRNQATWMFCRVPSQISDPAQRIRTIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMRIL
PHISITHSPAYNLILSNVPGPQAQLYFLGCRMDSMFPLGPLLGNAGLNITVMSLNGELGVGIVSCPDLLPDLWGVADGFP
EALKELLECSDDQPEGSNHQDS
>Mature_501_residues
PRGCAGARFACNACLNFLAGLGISEPISPGWAAMERLSGLDAFFLYMETPSQPLNVCCVLELDTSTMPGGYTYGRFHAAL
EKYVKAAPEFRMKLADTELNLDHPVWVDDDNFQIRHHLRRVAMPAPGGRRELAEICGYIAGLPLDRDRPLWEMWVIEGGA
RSDTVAVMLKVHHAVVDGVAGANLLSHLCSLQPDAPAPQPVRGTGGGNVLQIAASGLEGFASRPVRLATVVPATVLTLVR
TLLRAREGRTMAAPFSAPPTPFNGPLGRLRNIAYTQLDMRDVKRVKDRFGVTINDVVVALCAGALRRFLLEHGVLPEAPL
VATVPVSVHDKSDRPGRNQATWMFCRVPSQISDPAQRIRTIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMRILP
HISITHSPAYNLILSNVPGPQAQLYFLGCRMDSMFPLGPLLGNAGLNITVMSLNGELGVGIVSCPDLLPDLWGVADGFPE
ALKELLECSDDQPEGSNHQDS

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1760_MYCTU (O06795)

Other databases:

- EMBL:   BX842577
- EMBL:   AE000516
- PIR:   A70988
- RefSeq:   NP_216276.1
- RefSeq:   NP_336266.1
- ProteinModelPortal:   O06795
- EnsemblBacteria:   EBMYCT00000001977
- EnsemblBacteria:   EBMYCT00000072396
- GeneID:   885556
- GeneID:   923743
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1809
- KEGG:   mtu:Rv1760
- TIGR:   MT1809
- TubercuList:   Rv1760
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   RNQTTWM
- ProtClustDB:   CLSK791376
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 54093; Mature: 53962

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGCAGARFACNACLNFLAGLGISEPISPGWAAMERLSGLDAFFLYMETPSQPLNVCCV
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEE
LELDTSTMPGGYTYGRFHAALEKYVKAAPEFRMKLADTELNLDHPVWVDDDNFQIRHHLR
EEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCEEECCCCHHHHHHHH
RVAMPAPGGRRELAEICGYIAGLPLDRDRPLWEMWVIEGGARSDTVAVMLKVHHAVVDGV
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AGANLLSHLCSLQPDAPAPQPVRGTGGGNVLQIAASGLEGFASRPVRLATVVPATVLTLV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCEEEEHHHHHHHHHHH
RTLLRAREGRTMAAPFSAPPTPFNGPLGRLRNIAYTQLDMRDVKRVKDRFGVTINDVVVA
HHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LCAGALRRFLLEHGVLPEAPLVATVPVSVHDKSDRPGRNQATWMFCRVPSQISDPAQRIR
HHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCHHHCCHHHHHH
TIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMRILPHISITHSPAYNLILSNVPG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHEEEECCCC
PQAQLYFLGCRMDSMFPLGPLLGNAGLNITVMSLNGELGVGIVSCPDLLPDLWGVADGFP
CCEEEEEEEECCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEECHHHHHHHHHHCCCHH
EALKELLECSDDQPEGSNHQDS
HHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRGCAGARFACNACLNFLAGLGISEPISPGWAAMERLSGLDAFFLYMETPSQPLNVCCV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEE
LELDTSTMPGGYTYGRFHAALEKYVKAAPEFRMKLADTELNLDHPVWVDDDNFQIRHHLR
EEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCEEECCCCHHHHHHHH
RVAMPAPGGRRELAEICGYIAGLPLDRDRPLWEMWVIEGGARSDTVAVMLKVHHAVVDGV
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AGANLLSHLCSLQPDAPAPQPVRGTGGGNVLQIAASGLEGFASRPVRLATVVPATVLTLV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCEEEEHHHHHHHHHHH
RTLLRAREGRTMAAPFSAPPTPFNGPLGRLRNIAYTQLDMRDVKRVKDRFGVTINDVVVA
HHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LCAGALRRFLLEHGVLPEAPLVATVPVSVHDKSDRPGRNQATWMFCRVPSQISDPAQRIR
HHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCHHHCCHHHHHH
TIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMRILPHISITHSPAYNLILSNVPG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHEEEECCCC
PQAQLYFLGCRMDSMFPLGPLLGNAGLNITVMSLNGELGVGIVSCPDLLPDLWGVADGFP
CCEEEEEEEECCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEECHHHHHHHHHHCCCHH
EALKELLECSDDQPEGSNHQDS
HHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036