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The map label for this gene is pbp [H]

Identifier: 148661528

GI number: 148661528

Start: 1957210

End: 1958763

Strand: Reverse

Name: pbp [H]

Synonym: MRA_1740

Alternate gene names: 148661528

Gene position: 1958763-1957210 (Counterclockwise)

Preceding gene: 148661530

Following gene: 148661527

Centisome position: 44.32

GC content: 61.2

Gene sequence:

>1554_bases
TTGTGTCCGCCGATCATCTTGAGTTCCGCGACGCCGACCGGCACGCGGTGCGGGACGCGCCATGGGCGCGCGGTCGTCAC
CGAATACGTGCGTGCGCTAGATCGACTGCCGCACGAAATCGCCACTGCAGTGGTTGAAACTGTCAACTGCGCAGACCCTG
GTGCAGCGTTCGACGAACTCGATGCAAAAATCAACGCGGGCATGAAGGCCTATGCGATTCCGGGCGTCGCGGTTGCTGTC
TGGGCCGGCGGGCAAGAATACGTCAAAGGCTACGGGGTCACCAATGTCGACCATCCGATGCCTGTTGACGGCGACACTGT
CTTCAGAATCGGTTCCACCACAAAGACTTTCACAGGTACGGTGATGATGCGGCTGGTCGAGCGAGGCAAGGTGGACCTGG
ATTCACCTGTGCGCCGCTACATCCCCGACTTCGCGGTAGCCGACGAATCAGCCAGCGCTACGGTTACCGTTCGCCAACTG
CTCAACCATACCGCAGGCTGGGATGGTCGCAATGGGCAGGACTTTGGGCGCGGCGATGACGCGGTGGCGCTCTATGTCAA
GGCGATGACACGCCTACCGCAGCTCACCCCTCCGGGAACCGCGTTCGCGTACAACAATTCAGGTCTTGTGGTTGCGGGCC
GCATCATCGAGCTTGTCGCCGGAACAACCTACGAATCTACGGTTCAGAGGCTGTTGCTTGACCCGCTGCAGCTTGCTCAC
ACGCGCTACTTTTCCGACCAAATAATCGGTCTGAATGTGGCCGCATCGCATAGCGTGGTCGACGGCAAACCGATTGCCGT
TACTGACTTTTGGACATTCCCGCGCAGCTGCAACCCCACCGGTGGGTTGATGTCCACAGCGCGAGATCAGCTGCGTTACG
CACAGTTCCACCTCGGCGACGGCAGGGCGCCTAACGGTGAGCAGATTCTGAGCCGACAATCGCTGAAGGCAATGCGCTCT
AACCCTGGGGCGGGCGGAACACTTTGGGTGGAACTGACCGGGATGGGCGTGACCTGGATGCTGCGGCCCTCCGCGGAGAA
TGTGACCATCGTTGAGCACGGCGGCACCTGGAAGGGGCAGCGCTCTGGGTTCGTCATGGTGCCCGATCGAAACTTCGCCA
TGACCGTGCTCACTAACTCTGATGGCGGATTTCATATGATCAACGACCTTTTCGCATCCGACTGGGCATTGCAGAGATTC
GCCGGGCTCAGCAATCTTCCGGCCACGCCGCAACGCCTTGGTGCCGTCGACCTGGCGCCCTACGAGGGCCGGTACATCGC
CAAGCAAGTCGCCCAAAATGGCGACCTCGAGACAACGGTCATCGACTTCCGGGCCAGGGACGGCCAGCTTGCTGGAAGCA
TGAGCACCGACGATGCCAACCCGGATGGCCAAAACAGCGCCAATCTGGGCCTCGCCTTCTATCGGCCCGACTATGGGCTC
GACCTTGGACCCGACAACAAGCCCACCGGCAGTCGCTCCAACTTCGTGCGCGGGCCGGACGGCAACATCGCCTGGTTCTG
TAGCCAGCACGGCCGTCTGTTCCGACGCCAATAG

Upstream 100 bases:

>100_bases
CCGCGGAAGGCCAAAGCCATGCTTTCCGCGTTGGTTGACGAAGCGGATCCCCCCACGAAGCTGTTCAGATCGGCCGCCAC
CGGCCGGGTCGCCGCGGTCC

Downstream 100 bases:

>100_bases
CACAGCACCAGCCGGCTTGGTTTGCGGCGCGAGGAGGAACCGGGCAATCAGACGACGCCCGGTGACGATGCGCGCCGTTT
GCGGCGCGAGGAGGAACCGG

Product: putative penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP-4 [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDELDAKINAGMKAYAIPGVAVAV
WAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVRQL
LNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELVAGTTYESTVQRLLLDPLQLAH
TRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLGDGRAPNGEQILSRQSLKAMRS
NPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQRF
AGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDANPDGQNSANLGLAFYRPDYGL
DLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ

Sequences:

>Translated_517_residues
MCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDELDAKINAGMKAYAIPGVAVAV
WAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVRQL
LNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELVAGTTYESTVQRLLLDPLQLAH
TRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLGDGRAPNGEQILSRQSLKAMRS
NPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQRF
AGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDANPDGQNSANLGLAFYRPDYGL
DLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
>Mature_517_residues
MCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDELDAKINAGMKAYAIPGVAVAV
WAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVRQL
LNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELVAGTTYESTVQRLLLDPLQLAH
TRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLGDGRAPNGEQILSRQSLKAMRS
NPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQRF
AGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDANPDGQNSANLGLAFYRPDYGL
DLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ

Specific function: Involved in cell wall biosynthesis and may also act as a sensor of external penicillins [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051233, Length=95, Percent_Identity=37.8947368421053, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI26051231, Length=95, Percent_Identity=37.8947368421053, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790593, Length=263, Percent_Identity=26.9961977186312, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786574, Length=357, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533026, Length=197, Percent_Identity=28.4263959390863, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17550934, Length=188, Percent_Identity=29.2553191489362, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 55802; Mature: 55802

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDEL
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHH
DAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGT
HHHHHCCEEEEECCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHH
VMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVRQLLNHTAGWDGRNGQDFGRGDD
HHHHHHHHCCCCCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELVAGTTYESTVQRLLLDPLQLAH
HHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLGD
HHHCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCC
GRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQ
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCC
RSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQRFAGLSNLPATPQRLGAVDLAP
CCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEECCC
YEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDANPDGQNSANLGLAFYRPDYGL
CCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
DLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDEL
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHH
DAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGT
HHHHHCCEEEEECCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHH
VMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVRQLLNHTAGWDGRNGQDFGRGDD
HHHHHHHHCCCCCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELVAGTTYESTVQRLLLDPLQLAH
HHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLGD
HHHCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCC
GRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQ
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCC
RSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQRFAGLSNLPATPQRLGAVDLAP
CCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEECCC
YEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDANPDGQNSANLGLAFYRPDYGL
CCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
DLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]