Click here to switch to the map view.

The map label for this gene is nylB [H]

Identifier: 148661521

GI number: 148661521

Start: 1950860

End: 1952107

Strand: Direct

Name: nylB [H]

Synonym: MRA_1733

Alternate gene names: 148661521

Gene position: 1950860-1952107 (Clockwise)

Preceding gene: 148661520

Following gene: 148661524

Centisome position: 44.14

GC content: 69.07

Gene sequence:

>1248_bases
GTGAGCGGCGGCGTCCCGGCCGGTCTCGCACTGGACAACTGGCTGTCGTCGCCGTATTCGCATTGGGCATTCCAGCACGT
CGAAGACTTCATGCCGACCACGGTCATCGCGCGCGGCACCGAGCCGGTCGTGACGTTGCCCGCGGACAATGCGCCGATCG
CCGACATCGGCTTGACCAGCACGGACGGGATCGCCACCACCGTGGGCGCGGTGATGGCCGCCACCGCTACCGACGGGTGG
GCGGTCGCGCATCGCGGTGCGCTGGTGGCCGAGCAGTACCTCGACGGCCTGGGACCCCGGACCCGCCACCTGCTGTTCTC
GGTGAGCAAGTCGCTGGTGGCGGCTGTGGTCGGCGCGCTGCACGGGGCCGGGGCGATCGAGCTTGACGCGCCGGTCACGG
CGTACGTGCCCGCCTTGGCGGACTGCGGCTACGCCGGTGCGACGGTGCGCCACCTGCTGGACATGCGATCGGGTGTCGCC
TTCTCGGAGAACTACGACGACCCGGCCGCCGAGATTCACGTGCGCGAGCAGGTGATCGGGTGGGCGCCCAAGCGCGGTCC
GGACCTGCCCGCCACGCTGCGCGACTACCTGCTGACCTTGCGGCGGAAGTCGGCGCACGGCGGCCCGTTCGAATATCGCT
CGTGTGAAACCGACGTCCTCGGCTGGATCTGCGAGGCCGCGGCCGGACAGCCGATGCCCGAACTGATGTCGGAACTACTG
TGGAGCCGCATCGGGGCCCAGTGCGATGCCACCATCGCCCTAGACGTAGCCGGCGCGGCGGGCACCGGAATATTCGACGG
CGGCATCAGCGCCTGTCTGACCGACATGATCCGGTTCGGGTCGCTGTACCTGCGCGACGGTGTCTCGTTGGCCGGCCAGC
AAGTGGTGCCCGCGGCCTGGATCGCCGACACCTTCGACGGCGGCCCCGACTCGCGTCAGGCGTTCGCCGCCAGCCCCGAC
GACAACCCGATGCCCGGCGGGATGTACCGCAACCAAGTGTGGTTTCCCTACCCGGGCAGCAATGTCGCGTTGTGCGTGGG
CATGTGCGGCCAGCTGATCTACGTCAACCGCGCCGCGGAGGTGGTCGCCGCCAAGCTGTCCACCCAGCCGCACTCCCATG
AGCCGCACATGTTAGACACCCTGCGCGCATTCGATGCGGTGGCACACGAATTGTCAGGAATCAGATCGAGTTCGACCAAC
GACCCGCAGCGGCCTTCCCCGCCAGCCCAGGAGGCCAGTCCGGGGTAA

Upstream 100 bases:

>100_bases
CCGAGCGCGGCCGGCGCTGGATCGACGGCCTCAAGGCGCAGTTCGCCTCGACGCCGCTGACGCCCGCCGCCCCGATCGTC
TCGCGGCTCGTCGCACGGGC

Downstream 100 bases:

>100_bases
CGGCTTGTGCCCACGTAACCGAGTTCCAGGGCGATGGGCTTATTAGCGGAAATATGACTCGTCCCAGGTATCCATACGAC
GCTTGCGTACCTCGGCGAGC

Product: putative hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSGGVPAGLALDNWLSSPYSHWAFQHVEDFMPTTVIARGTEPVVTLPADNAPIADIGLTSTDGIATTVGAVMAATATDGW
AVAHRGALVAEQYLDGLGPRTRHLLFSVSKSLVAAVVGALHGAGAIELDAPVTAYVPALADCGYAGATVRHLLDMRSGVA
FSENYDDPAAEIHVREQVIGWAPKRGPDLPATLRDYLLTLRRKSAHGGPFEYRSCETDVLGWICEAAAGQPMPELMSELL
WSRIGAQCDATIALDVAGAAGTGIFDGGISACLTDMIRFGSLYLRDGVSLAGQQVVPAAWIADTFDGGPDSRQAFAASPD
DNPMPGGMYRNQVWFPYPGSNVALCVGMCGQLIYVNRAAEVVAAKLSTQPHSHEPHMLDTLRAFDAVAHELSGIRSSSTN
DPQRPSPPAQEASPG

Sequences:

>Translated_415_residues
MSGGVPAGLALDNWLSSPYSHWAFQHVEDFMPTTVIARGTEPVVTLPADNAPIADIGLTSTDGIATTVGAVMAATATDGW
AVAHRGALVAEQYLDGLGPRTRHLLFSVSKSLVAAVVGALHGAGAIELDAPVTAYVPALADCGYAGATVRHLLDMRSGVA
FSENYDDPAAEIHVREQVIGWAPKRGPDLPATLRDYLLTLRRKSAHGGPFEYRSCETDVLGWICEAAAGQPMPELMSELL
WSRIGAQCDATIALDVAGAAGTGIFDGGISACLTDMIRFGSLYLRDGVSLAGQQVVPAAWIADTFDGGPDSRQAFAASPD
DNPMPGGMYRNQVWFPYPGSNVALCVGMCGQLIYVNRAAEVVAAKLSTQPHSHEPHMLDTLRAFDAVAHELSGIRSSSTN
DPQRPSPPAQEASPG
>Mature_414_residues
SGGVPAGLALDNWLSSPYSHWAFQHVEDFMPTTVIARGTEPVVTLPADNAPIADIGLTSTDGIATTVGAVMAATATDGWA
VAHRGALVAEQYLDGLGPRTRHLLFSVSKSLVAAVVGALHGAGAIELDAPVTAYVPALADCGYAGATVRHLLDMRSGVAF
SENYDDPAAEIHVREQVIGWAPKRGPDLPATLRDYLLTLRRKSAHGGPFEYRSCETDVLGWICEAAAGQPMPELMSELLW
SRIGAQCDATIALDVAGAAGTGIFDGGISACLTDMIRFGSLYLRDGVSLAGQQVVPAAWIADTFDGGPDSRQAFAASPDD
NPMPGGMYRNQVWFPYPGSNVALCVGMCGQLIYVNRAAEVVAAKLSTQPHSHEPHMLDTLRAFDAVAHELSGIRSSSTND
PQRPSPPAQEASPG

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 43631; Mature: 43499

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGVPAGLALDNWLSSPYSHWAFQHVEDFMPTTVIARGTEPVVTLPADNAPIADIGLTS
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHEEECCCCCEEEECCCCCCCCCCCCCC
TDGIATTVGAVMAATATDGWAVAHRGALVAEQYLDGLGPRTRHLLFSVSKSLVAAVVGAL
CCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HGAGAIELDAPVTAYVPALADCGYAGATVRHLLDMRSGVAFSENYDDPAAEIHVREQVIG
HCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
WAPKRGPDLPATLRDYLLTLRRKSAHGGPFEYRSCETDVLGWICEAAAGQPMPELMSELL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
WSRIGAQCDATIALDVAGAAGTGIFDGGISACLTDMIRFGSLYLRDGVSLAGQQVVPAAW
HHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCHHCCCCCCCHHH
IADTFDGGPDSRQAFAASPDDNPMPGGMYRNQVWFPYPGSNVALCVGMCGQLIYVNRAAE
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHCCEEEHHHHHH
VVAAKLSTQPHSHEPHMLDTLRAFDAVAHELSGIRSSSTNDPQRPSPPAQEASPG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGGVPAGLALDNWLSSPYSHWAFQHVEDFMPTTVIARGTEPVVTLPADNAPIADIGLTS
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHEEECCCCCEEEECCCCCCCCCCCCCC
TDGIATTVGAVMAATATDGWAVAHRGALVAEQYLDGLGPRTRHLLFSVSKSLVAAVVGAL
CCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HGAGAIELDAPVTAYVPALADCGYAGATVRHLLDMRSGVAFSENYDDPAAEIHVREQVIG
HCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
WAPKRGPDLPATLRDYLLTLRRKSAHGGPFEYRSCETDVLGWICEAAAGQPMPELMSELL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
WSRIGAQCDATIALDVAGAAGTGIFDGGISACLTDMIRFGSLYLRDGVSLAGQQVVPAAW
HHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCHHCCCCCCCHHH
IADTFDGGPDSRQAFAASPDDNPMPGGMYRNQVWFPYPGSNVALCVGMCGQLIYVNRAAE
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHCCEEEHHHHHH
VVAAKLSTQPHSHEPHMLDTLRAFDAVAHELSGIRSSSTNDPQRPSPPAQEASPG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]