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The map label for this gene is pheT [H]

Identifier: 148661446

GI number: 148661446

Start: 1861276

End: 1863771

Strand: Direct

Name: pheT [H]

Synonym: MRA_1661

Alternate gene names: 148661446

Gene position: 1861276-1863771 (Clockwise)

Preceding gene: 148661445

Following gene: 148661448

Centisome position: 42.11

GC content: 68.95

Gene sequence:

>2496_bases
ATGCGGCTACCCTACAGCTGGCTGCGCGAGGTGGTTGCGGTCGGCGCTTCGGGCTGGGACGTTACCCCAGGCGAACTCGA
GCAGACGCTGTTGCGCATCGGCCACGAGGTCGAAGAGGTCATCCCCCTTGGTCCGGTGGACGGCCCGGTGACCGTGGGGC
GGGTGGCCGATATCGAGGAGCTCACCGGCTACAAGAAGCCGATCCGGGCCTGCGCGGTAGATATCGGCGATCGGCAGTAT
CGCGAGATTATTTGTGGTGCAACCAATTTCGCGGTTGGTGATCTGGTGGTGGTAGCGCTGCCCGGTGCCACGCTGCCCGG
TGGATTCACCATTAGCGCCCGCAAGGCCTACGGTCGCAACTCCGACGGAATGATCTGCTCGGCAGCCGAACTCAATTTGG
GCGCAGACCATTCCGGGATCCTGGTGTTGCCCCCCGGAGCCGCCGAGCCCGGAGCTGACGGCGCGGGCGTGCTGGGGCTC
GACGACGTGGTCTTCCATCTGGCCATCACCCCAGACCGCGGTTACTGCATGTCGGTGCGCGGCTTGGCCCGCGAGCTCGC
GTGCGCCTACGACCTGGACTTCGTCGACCCCGCCAGCAACTCGCGGGTGCCGCCGCTACCCATCGAGGGGCCAGCCTGGC
CGCTGACGGTTCAGCCCGAGACGGGGGTGCGCCGGTTCGCGCTACGCCCGGTCATCGGGATCGACCCCGCCGCGGTATCG
CCCTGGTGGTTGCAGCGCCGACTGCTGCTCTGCGGTATCCGCGCGACCTGTCCGGCGGTCGACGTGACCAATTACGTGAT
GCTCGAACTTGGCCACCCCATGCACGCCCACGACCGCAACCGGATCAGCGGAACCCTCGGAGTGCGGTTCGCCCGGTCCG
GCGAGACCGCCGTGACCCTCGACGGTATCGAGCGCAAGCTCGATACCGCCGATGTCCTGATCGTCGACGATGCTGCGACA
GCGGCGATCGGCGGCGTGATGGGGGCGGCCAGCACCGAAGTGCGGGCCGACTCCACCGATGTCCTGTTGGAGGCCGCGAT
ATGGGACCCGGCTGCGGTATCGCGTACCCAGCGGCGGCTGCACCTGCCTAGCGAGGCCGCCCGTCGTTACGAGCGGACGG
TGGACCCGGCCATCTCCGTGGCCGCTTTGGACCGGTGCGCAAGGCTGCTCGCCGACATCGCCGGGGGGGAGGTTTCTCCC
ACCCTTACCGACTGGCGGGGTGACCCGCCGTGTGATGACTGGTCACCGCCGCCGATCCGGATGGGAGTCGATGTGCCGGA
CCGCATCGCCGGGGTGGCCTATCCGCAGGGCACTACTGCCAGGCGCTTGGCCCAGATCGGCGCGGTGGTGACCCACGACG
GCGACACCTTGACCGTGACCCCGCCGAGTTGGCGACCTGATCTGCGGCAACCCGCAGACCTTGTCGAGGAGGTGCTGCGG
CTTGAGGGGCTGGAAGTTATCCCGTCGGTGCTGCCACCGGCGCCCGCGGGTCGTGGACTCACCGCTGGGCAGCAGCGCCG
TCGCACGATCGGCAGGTCGCTGGCGCTGTCGGGCTATGTCGAGATTCTGCCGACTCCATTTCTGCCGGCCGGTGTGTTCG
ATTTGTGGGGGCTGGAAGCCGATGACTCACGGCGCATGACCACGCGGGTGCTCAACCCGCTGGAGGCCGATCGTCCGCAA
CTGGCGACCACGCTGCTGCCGGCCCTGCTGGAAGCCTTGGTGCGCAACGTGTCCCGAGGGCTGGTCGACGTCGCGCTGTT
CGCCATCGCCCAGGTGGTCCAGCCGACCGAGCAGACGCGCGGTGTCGGGTTGATCCCGGTTGACCGGCGGCCGACCGATG
ATGAGATCGCCATGCTGGATGCCTCGCTGCCCCGGCAACCCCAGCACGTCGCGGCGGTGCTGGCCGGACTGCGCGAGCCT
CGAGGCCCCTGGGGCCCGGGCCGCCCGGTAGAGGCGGCTGATGCGTTCGAGGCGGTGCGAATCATCGCGCGCGCCAGCCG
CGTGGACGTGACCCTGCGGCCGGCCCAATATCTGCCGTGGCATCCGGGCCGGTGCGCGCAGGTGTTCGTCGGGGAAAGCT
CGGTTGGTCACGCCGGGCAGCTGCATCCCGCCGTGATCGAGCGCTCGGGTCTGCCGAAAGGCACCTGCGCGGTGGAACTG
AACCTAGATGCGATTCCGTGCAGCGCGCCGCTGCCGGCACCCAGGGTGTCGCCGTATCCGGCCGTGTTCCAAGACGTCAG
CCTGGTGGTGGCCGCGGACATCCCCGCTCAGGCGGTGGCCGACGCCGTGCGCGCGGGGGCAGGCGACCTGCTGGAGGATA
TTGCGTTGTTTGACGTGTTCACCGGCCCGCAGATTGGTGAGCACCGCAAGTCGCTGACCTTCGCGCTGCGGTTTCGTGCG
CCGGATCGCACCTTAACCGAAGACGACGCCAGCGCCGCCCGCGATGCCGCTGTGCAAAGCGCAGCCGAACGGGTGGGTGC
CGTGCTGCGTGGCTGA

Upstream 100 bases:

>100_bases
GGTTGGAACGCACCCTGCAGTTTCGCAACGGCATTCCTGACATGCGCGACATGGTCGAAGGCGACGTCCGATTCTCGTTG
CCGTTCGGGGTGGGTGCCTG

Downstream 100 bases:

>100_bases
ACCGACTCAGCACGCGTTCAACGAAAATTTGACGACGGCATTTCAGCGCGCCGCGTTTATACCTCGCCGCCCTGTCCGGG
TAGCGGCGCCGCCCTAAGGG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MRLPYSWLREVVAVGASGWDVTPGELEQTLLRIGHEVEEVIPLGPVDGPVTVGRVADIEELTGYKKPIRACAVDIGDRQY
REIICGATNFAVGDLVVVALPGATLPGGFTISARKAYGRNSDGMICSAAELNLGADHSGILVLPPGAAEPGADGAGVLGL
DDVVFHLAITPDRGYCMSVRGLARELACAYDLDFVDPASNSRVPPLPIEGPAWPLTVQPETGVRRFALRPVIGIDPAAVS
PWWLQRRLLLCGIRATCPAVDVTNYVMLELGHPMHAHDRNRISGTLGVRFARSGETAVTLDGIERKLDTADVLIVDDAAT
AAIGGVMGAASTEVRADSTDVLLEAAIWDPAAVSRTQRRLHLPSEAARRYERTVDPAISVAALDRCARLLADIAGGEVSP
TLTDWRGDPPCDDWSPPPIRMGVDVPDRIAGVAYPQGTTARRLAQIGAVVTHDGDTLTVTPPSWRPDLRQPADLVEEVLR
LEGLEVIPSVLPPAPAGRGLTAGQQRRRTIGRSLALSGYVEILPTPFLPAGVFDLWGLEADDSRRMTTRVLNPLEADRPQ
LATTLLPALLEALVRNVSRGLVDVALFAIAQVVQPTEQTRGVGLIPVDRRPTDDEIAMLDASLPRQPQHVAAVLAGLREP
RGPWGPGRPVEAADAFEAVRIIARASRVDVTLRPAQYLPWHPGRCAQVFVGESSVGHAGQLHPAVIERSGLPKGTCAVEL
NLDAIPCSAPLPAPRVSPYPAVFQDVSLVVAADIPAQAVADAVRAGAGDLLEDIALFDVFTGPQIGEHRKSLTFALRFRA
PDRTLTEDDASAARDAAVQSAAERVGAVLRG

Sequences:

>Translated_831_residues
MRLPYSWLREVVAVGASGWDVTPGELEQTLLRIGHEVEEVIPLGPVDGPVTVGRVADIEELTGYKKPIRACAVDIGDRQY
REIICGATNFAVGDLVVVALPGATLPGGFTISARKAYGRNSDGMICSAAELNLGADHSGILVLPPGAAEPGADGAGVLGL
DDVVFHLAITPDRGYCMSVRGLARELACAYDLDFVDPASNSRVPPLPIEGPAWPLTVQPETGVRRFALRPVIGIDPAAVS
PWWLQRRLLLCGIRATCPAVDVTNYVMLELGHPMHAHDRNRISGTLGVRFARSGETAVTLDGIERKLDTADVLIVDDAAT
AAIGGVMGAASTEVRADSTDVLLEAAIWDPAAVSRTQRRLHLPSEAARRYERTVDPAISVAALDRCARLLADIAGGEVSP
TLTDWRGDPPCDDWSPPPIRMGVDVPDRIAGVAYPQGTTARRLAQIGAVVTHDGDTLTVTPPSWRPDLRQPADLVEEVLR
LEGLEVIPSVLPPAPAGRGLTAGQQRRRTIGRSLALSGYVEILPTPFLPAGVFDLWGLEADDSRRMTTRVLNPLEADRPQ
LATTLLPALLEALVRNVSRGLVDVALFAIAQVVQPTEQTRGVGLIPVDRRPTDDEIAMLDASLPRQPQHVAAVLAGLREP
RGPWGPGRPVEAADAFEAVRIIARASRVDVTLRPAQYLPWHPGRCAQVFVGESSVGHAGQLHPAVIERSGLPKGTCAVEL
NLDAIPCSAPLPAPRVSPYPAVFQDVSLVVAADIPAQAVADAVRAGAGDLLEDIALFDVFTGPQIGEHRKSLTFALRFRA
PDRTLTEDDASAARDAAVQSAAERVGAVLRG
>Mature_831_residues
MRLPYSWLREVVAVGASGWDVTPGELEQTLLRIGHEVEEVIPLGPVDGPVTVGRVADIEELTGYKKPIRACAVDIGDRQY
REIICGATNFAVGDLVVVALPGATLPGGFTISARKAYGRNSDGMICSAAELNLGADHSGILVLPPGAAEPGADGAGVLGL
DDVVFHLAITPDRGYCMSVRGLARELACAYDLDFVDPASNSRVPPLPIEGPAWPLTVQPETGVRRFALRPVIGIDPAAVS
PWWLQRRLLLCGIRATCPAVDVTNYVMLELGHPMHAHDRNRISGTLGVRFARSGETAVTLDGIERKLDTADVLIVDDAAT
AAIGGVMGAASTEVRADSTDVLLEAAIWDPAAVSRTQRRLHLPSEAARRYERTVDPAISVAALDRCARLLADIAGGEVSP
TLTDWRGDPPCDDWSPPPIRMGVDVPDRIAGVAYPQGTTARRLAQIGAVVTHDGDTLTVTPPSWRPDLRQPADLVEEVLR
LEGLEVIPSVLPPAPAGRGLTAGQQRRRTIGRSLALSGYVEILPTPFLPAGVFDLWGLEADDSRRMTTRVLNPLEADRPQ
LATTLLPALLEALVRNVSRGLVDVALFAIAQVVQPTEQTRGVGLIPVDRRPTDDEIAMLDASLPRQPQHVAAVLAGLREP
RGPWGPGRPVEAADAFEAVRIIARASRVDVTLRPAQYLPWHPGRCAQVFVGESSVGHAGQLHPAVIERSGLPKGTCAVEL
NLDAIPCSAPLPAPRVSPYPAVFQDVSLVVAADIPAQAVADAVRAGAGDLLEDIALFDVFTGPQIGEHRKSLTFALRFRA
PDRTLTEDDASAARDAAVQSAAERVGAVLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=775, Percent_Identity=31.4838709677419, Blast_Score=332, Evalue=8e-92,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88375; Mature: 88375

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50886 TRBD ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPYSWLREVVAVGASGWDVTPGELEQTLLRIGHEVEEVIPLGPVDGPVTVGRVADIEE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCEEECCCCCHHH
LTGYKKPIRACAVDIGDRQYREIICGATNFAVGDLVVVALPGATLPGGFTISARKAYGRN
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHCCCC
SDGMICSAAELNLGADHSGILVLPPGAAEPGADGAGVLGLDDVVFHLAITPDRGYCMSVR
CCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCEEHHH
GLARELACAYDLDFVDPASNSRVPPLPIEGPAWPLTVQPETGVRRFALRPVIGIDPAAVS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCC
PWWLQRRLLLCGIRATCPAVDVTNYVMLELGHPMHAHDRNRISGTLGVRFARSGETAVTL
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEE
DGIERKLDTADVLIVDDAATAAIGGVMGAASTEVRADSTDVLLEAAIWDPAAVSRTQRRL
CCHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHEEEEHHCCCHHHHHHHHHC
HLPSEAARRYERTVDPAISVAALDRCARLLADIAGGEVSPTLTDWRGDPPCDDWSPPPIR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEE
MGVDVPDRIAGVAYPQGTTARRLAQIGAVVTHDGDTLTVTPPSWRPDLRQPADLVEEVLR
ECCCCCHHHHCCCCCCCHHHHHHHHHCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHH
LEGLEVIPSVLPPAPAGRGLTAGQQRRRTIGRSLALSGYVEILPTPFLPAGVFDLWGLEA
HCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHCCCCC
DDSRRMTTRVLNPLEADRPQLATTLLPALLEALVRNVSRGLVDVALFAIAQVVQPTEQTR
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GVGLIPVDRRPTDDEIAMLDASLPRQPQHVAAVLAGLREPRGPWGPGRPVEAADAFEAVR
CCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
IIARASRVDVTLRPAQYLPWHPGRCAQVFVGESSVGHAGQLHPAVIERSGLPKGTCAVEL
HHHHHHHCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEE
NLDAIPCSAPLPAPRVSPYPAVFQDVSLVVAADIPAQAVADAVRAGAGDLLEDIALFDVF
ECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHC
TGPQIGEHRKSLTFALRFRAPDRTLTEDDASAARDAAVQSAAERVGAVLRG
CCCCHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLPYSWLREVVAVGASGWDVTPGELEQTLLRIGHEVEEVIPLGPVDGPVTVGRVADIEE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCEEECCCCCHHH
LTGYKKPIRACAVDIGDRQYREIICGATNFAVGDLVVVALPGATLPGGFTISARKAYGRN
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHCCCC
SDGMICSAAELNLGADHSGILVLPPGAAEPGADGAGVLGLDDVVFHLAITPDRGYCMSVR
CCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCEEHHH
GLARELACAYDLDFVDPASNSRVPPLPIEGPAWPLTVQPETGVRRFALRPVIGIDPAAVS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCC
PWWLQRRLLLCGIRATCPAVDVTNYVMLELGHPMHAHDRNRISGTLGVRFARSGETAVTL
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEE
DGIERKLDTADVLIVDDAATAAIGGVMGAASTEVRADSTDVLLEAAIWDPAAVSRTQRRL
CCHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHEEEEHHCCCHHHHHHHHHC
HLPSEAARRYERTVDPAISVAALDRCARLLADIAGGEVSPTLTDWRGDPPCDDWSPPPIR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEE
MGVDVPDRIAGVAYPQGTTARRLAQIGAVVTHDGDTLTVTPPSWRPDLRQPADLVEEVLR
ECCCCCHHHHCCCCCCCHHHHHHHHHCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHH
LEGLEVIPSVLPPAPAGRGLTAGQQRRRTIGRSLALSGYVEILPTPFLPAGVFDLWGLEA
HCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHCCCCC
DDSRRMTTRVLNPLEADRPQLATTLLPALLEALVRNVSRGLVDVALFAIAQVVQPTEQTR
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GVGLIPVDRRPTDDEIAMLDASLPRQPQHVAAVLAGLREPRGPWGPGRPVEAADAFEAVR
CCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
IIARASRVDVTLRPAQYLPWHPGRCAQVFVGESSVGHAGQLHPAVIERSGLPKGTCAVEL
HHHHHHHCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEE
NLDAIPCSAPLPAPRVSPYPAVFQDVSLVVAADIPAQAVADAVRAGAGDLLEDIALFDVF
ECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHC
TGPQIGEHRKSLTFALRFRAPDRTLTEDDASAARDAAVQSAAERVGAVLRG
CCCCHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]