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The map label for this gene is tsnR [H]

Identifier: 148661440

GI number: 148661440

Start: 1855124

End: 1855906

Strand: Direct

Name: tsnR [H]

Synonym: MRA_1655

Alternate gene names: 148661440

Gene position: 1855124-1855906 (Clockwise)

Preceding gene: 148661439

Following gene: 148661442

Centisome position: 41.97

GC content: 69.6

Gene sequence:

>783_bases
GTGCTCACCGAACGCTCGGCCAGGGTGGCCACGGCGGTCAAACTGCATCGTCACGTAGGCCGGCGCCGGGCGGGACGTTT
TCTCGCCGAAGGCCCCAACCTGGTAGCGGCGGCGTTGGCGCGCGGGCTGGTACGGGAGGTATTCGTCACCGAAGTTGCGG
CGCGGCGGCACGAGCTCTTGTTGGCCGCGCACGAGGCTTCGGTTCATCTGGTGACTGAGCGGGCCGCGAAGGCGCTCTCT
GATACGGTCACGCCGGCCGGGTTGGTGGCGGTGTGCGATCTGCCGGCGACCCGACTTGAGGATGTATTGGCCGGCTCACC
TCAGCTGATCGCGGTGACCGTCGAGATCCGCGAGCCGGGCAACGCGGGCACGGTAATCCGCATCGCCGACGCCATGGGTG
CCGCGGCGGTGATCCTCGCCGGGCGCAGCGTCGACCCATACAACGGCAAGTGTCTGCGCGCGTCCACCGGTAGCATCTTC
GCGATCCCGGTCGTCGTCGCGCCCGATGTCGGTGCCGCCATCGCCGACCTGCGAGCGGCCGGACTGCAGGTGCTGGCCAC
CGCAGTGGACGGCGAGATGGCTCTCGACGATGCCGATCGGCTGCTTGCCGAGCCGACGGCATGGCTGTTCGGGCCCGAAG
CACACGGGTTGTCGGCCGAGATCGCGGCCTTGGCGGACCACCGCGTACACATCCTGATGTCGGGAGGGGCGGAGAGCCTC
AACGTCGCGGCCGCGGCCGCGATCTGTCTGTATGAGAGCGCTCGGGCGTTGGGCCGCCGCTGA

Upstream 100 bases:

>100_bases
CGCTGGTCGACGTCGCCCGGGCGGCACTGCCCGAAGACGTCAACGCCCCCTCCGGGGAGGCCGCCTGATCCGGATTCCGG
CCTGAGGCAGGGCTACGCCG

Downstream 100 bases:

>100_bases
TTGTCCGGCCCTACGCAGCGCGGCTGGGGCCCCGCGCCGGCCGCACGCCGGCCAGCGAAAGTGTGGAATGGACCAGCGCC
CCGGCGCGTTCCATCAGGGC

Product: putative 23S rRNA methyltransferase TsnR

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLTERSARVATAVKLHRHVGRRRAGRFLAEGPNLVAAALARGLVREVFVTEVAARRHELLLAAHEASVHLVTERAAKALS
DTVTPAGLVAVCDLPATRLEDVLAGSPQLIAVTVEIREPGNAGTVIRIADAMGAAAVILAGRSVDPYNGKCLRASTGSIF
AIPVVVAPDVGAAIADLRAAGLQVLATAVDGEMALDDADRLLAEPTAWLFGPEAHGLSAEIAALADHRVHILMSGGAESL
NVAAAAAICLYESARALGRR

Sequences:

>Translated_260_residues
MLTERSARVATAVKLHRHVGRRRAGRFLAEGPNLVAAALARGLVREVFVTEVAARRHELLLAAHEASVHLVTERAAKALS
DTVTPAGLVAVCDLPATRLEDVLAGSPQLIAVTVEIREPGNAGTVIRIADAMGAAAVILAGRSVDPYNGKCLRASTGSIF
AIPVVVAPDVGAAIADLRAAGLQVLATAVDGEMALDDADRLLAEPTAWLFGPEAHGLSAEIAALADHRVHILMSGGAESL
NVAAAAAICLYESARALGRR
>Mature_260_residues
MLTERSARVATAVKLHRHVGRRRAGRFLAEGPNLVAAALARGLVREVFVTEVAARRHELLLAAHEASVHLVTERAAKALS
DTVTPAGLVAVCDLPATRLEDVLAGSPQLIAVTVEIREPGNAGTVIRIADAMGAAAVILAGRSVDPYNGKCLRASTGSIF
AIPVVVAPDVGAAIADLRAAGLQVLATAVDGEMALDDADRLLAEPTAWLFGPEAHGLSAEIAALADHRVHILMSGGAESL
NVAAAAAICLYESARALGRR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=297, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1790083, Length=177, Percent_Identity=32.7683615819209, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666840, Length=289, Percent_Identity=28.0276816608997, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 26880; Mature: 26880

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTERSARVATAVKLHRHVGRRRAGRFLAEGPNLVAAALARGLVREVFVTEVAARRHELL
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
LAAHEASVHLVTERAAKALSDTVTPAGLVAVCDLPATRLEDVLAGSPQLIAVTVEIREPG
EEECCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCC
NAGTVIRIADAMGAAAVILAGRSVDPYNGKCLRASTGSIFAIPVVVAPDVGAAIADLRAA
CCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHH
GLQVLATAVDGEMALDDADRLLAEPTAWLFGPEAHGLSAEIAALADHRVHILMSGGAESL
HHHHEEECCCCCEECCHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCEEEEEEECCCCHH
NVAAAAAICLYESARALGRR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTERSARVATAVKLHRHVGRRRAGRFLAEGPNLVAAALARGLVREVFVTEVAARRHELL
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
LAAHEASVHLVTERAAKALSDTVTPAGLVAVCDLPATRLEDVLAGSPQLIAVTVEIREPG
EEECCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCC
NAGTVIRIADAMGAAAVILAGRSVDPYNGKCLRASTGSIFAIPVVVAPDVGAAIADLRAA
CCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHH
GLQVLATAVDGEMALDDADRLLAEPTAWLFGPEAHGLSAEIAALADHRVHILMSGGAESL
HHHHEEECCCCCEECCHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCEEEEEEECCCCHH
NVAAAAAICLYESARALGRR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]