Click here to switch to the map view.

The map label for this gene is epiA [H]

Identifier: 148661308

GI number: 148661308

Start: 1705610

End: 1706578

Strand: Direct

Name: epiA [H]

Synonym: MRA_1524

Alternate gene names: 148661308

Gene position: 1705610-1706578 (Clockwise)

Preceding gene: 148661307

Following gene: 148661309

Centisome position: 38.59

GC content: 67.6

Gene sequence:

>969_bases
ATGAACGCGCACACCTCGGTCGGCCCGCTTGACCGCGCGGCCCGGGTCTACATCGCCGGGCATCGCGGCCTGGTCGGGTC
CGCGCTGCTACGCACGTTTGCGGGCGCGGGGTTCACCAACCTGCTGGTGCGGTCACGCGCCGAGCTTGATCTGACGGATC
GGGCCGCGACGTTCGACTTCGTTCTCGAGTCGAGGCCGCAGGTCGTCATCGACGCGGCGGCCCGGGTCGGCGGCATCCTG
GCCAACGACACCTACCCGGCCGATTTCCTGTCGGAAAACCTCCAGATCCAGGTCAACCTGCTGGATGCCGCCGTGGCGGC
GCGGGTGCCGCGGCTGCTGTTCCTGGGCTCGTCGTGCATCTACCCGAAACTCGCCCCGCAGCCGATCCCGGAGAGCGCGC
TGCTCACCGGTCCGTTGGAGCCGACCAACGACGCGTACGCGATCGCCAAAATCGCCGGCATCCTTGCGGTCCAGGCGGTG
CGCCGCCAACATGGCCTGCCGTGGATCTCGGCGATGCCCACCAACCTGTACGGGCCAGGCGACAACTTTTCGCCGTCCGG
CTCGCATCTGCTGCCGGCACTCATCCGCCGCTATGACGAGGCCAAAGCCAGTGGCGCGCCCAACGTGACCAACTGGGGCA
CCGGCACGCCCCGACGGGAGTTGCTGCACGTCGACGACCTGGCGAGCGCATGCCTGTATCTGCTGGAACATTTCGACGGG
CCGACCCATGTCAACGTGGGAACCGGCATCGACCACACCATCGGCGAGATCGCCGAGATGGTCGCCTCGGCGGTAGGCTA
TAGCGGCGAAACCCGCTGGGATCCAAGCAAACCGGACGGAACACCACGCAAACTGCTGGATGTTTCGGTGCTACGGGAGG
CGGGATGGCGGCCTTCGATCGCGCTGCGCGACGGCATCGAGGCGACGGTGGCGTGGTATCGCGAGCACGCGGGAACGGTT
CGGCAATGA

Upstream 100 bases:

>100_bases
ACACTGACGAGTTGGCTCGGATCATGGTCGACGCGGACATGGCGGCGCTGGAGTGCGAAGGCAAGCCGTGGATCGACAAG
CCGATGATCGCCGGCCGGAC

Downstream 100 bases:

>100_bases
GGCTGGCCCGTCGCGCTCGGAACATCTTGCGTCGCAACGGCATCGAGGTGTCGCGCTACTTTGCCGAACTGGACTGGGAA
CGCAATTTCTTGCGCCAACT

Product: fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDFVLESRPQVVIDAAARVGGIL
ANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCIYPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAV
RRQHGLPWISAMPTNLYGPGDNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDG
PTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSIALRDGIEATVAWYREHAGTV
RQ

Sequences:

>Translated_322_residues
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDFVLESRPQVVIDAAARVGGIL
ANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCIYPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAV
RRQHGLPWISAMPTNLYGPGDNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDG
PTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSIALRDGIEATVAWYREHAGTV
RQ
>Mature_322_residues
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDFVLESRPQVVIDAAARVGGIL
ANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCIYPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAV
RRQHGLPWISAMPTNLYGPGDNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDG
PTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSIALRDGIEATVAWYREHAGTV
RQ

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=318, Percent_Identity=32.3899371069182, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI7657641, Length=224, Percent_Identity=24.1071428571429, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17554428, Length=317, Percent_Identity=26.1829652996845, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922778, Length=321, Percent_Identity=30.5295950155763, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34538; Mature: 34538

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDF
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHH
VLESRPQVVIDAAARVGGILANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCI
HHCCCCCEEEEHHHHHCCEEECCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
YPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAVRRQHGLPWISAMPTNLYGPG
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
DNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSI
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
ALRDGIEATVAWYREHAGTVRQ
HHHCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDF
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHH
VLESRPQVVIDAAARVGGILANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCI
HHCCCCCEEEEHHHHHCCEEECCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
YPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAVRRQHGLPWISAMPTNLYGPG
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
DNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSI
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
ALRDGIEATVAWYREHAGTVRQ
HHHCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]