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The map label for this gene is gmdA [H]

Identifier: 148661307

GI number: 148661307

Start: 1704591

End: 1705613

Strand: Direct

Name: gmdA [H]

Synonym: MRA_1523

Alternate gene names: 148661307

Gene position: 1704591-1705613 (Clockwise)

Preceding gene: 148661306

Following gene: 148661308

Centisome position: 38.57

GC content: 63.34

Gene sequence:

>1023_bases
GTGAAGCGAGCGCTCATCACCGGAATCACCGGCCAGGACGGCTCGTATCTCGCCGAACTGCTGCTGGCCAAGGGGTATGA
GGTTCACGGGCTCATCCGGCGCGCTTCGACGTTCAACACCTCGCGGATCGATCACCTCTACGTCGACCCGCACCAACCGG
GCGCGCGGCTGTTTCTGCACTATGGTGACCTGATCGACGGAACCCGGTTGGTGACCCTGCTGAGCACCATCGAACCCGAC
GAGGTGTACAACCTGGCGGCGCAGTCACACGTGCGGGTGAGCTTCGACGAACCCGTGCACACCGGTGACACCACCGGCAT
GGGATCCATGCGACTGCTGGAAGCCGTTCGGCTCTCTCGGGTGCACTGCCGCTTCTATCAGGCGTCCTCGTCGGAGATGT
TCGGCGCCTCGCCGCCACCGCAGAACGAGCTGACGCCGTTCTACCCGCGGTCACCGTATGGCGCCGCCAAGGTCTATTCG
TACTGGGCGACCCGCAATTATCGCGAAGCGTACGGATTGTTCGCCGTTAACGGCATCTTGTTCAATCACGAATCACCGCG
GCGCGGTGAGACGTTCGTGACCCGAAAGATCACCAGGGCCGTGGCACGCATCAAGGCCGGTATCCAGTCCGAGGTCTATA
TGGGCAATCTGGATGCGGTCCGCGACTGGGGGTACGCGCCCGAATACGTCGAAGGCATGTGGCGGATGCTGCAGACCGAC
GAGCCCGACGACTTCGTTTTGGCGACCGGGCGCGGTTTCACCGTGCGTGAGTTCGCGCGGGCCGCGTTCGAGCATGCCGG
TTTGGACTGGCAGCAGTACGTGAAATTCGACCAACGCTATCTGCGGCCCACCGAGGTGGATTCGCTGATCGGCGACGCGA
CCAAGGCTGCCGAATTGCTGGGCTGGAGGGCTTCGGTGCACACTGACGAGTTGGCTCGGATCATGGTCGACGCGGACATG
GCGGCGCTGGAGTGCGAAGGCAAGCCGTGGATCGACAAGCCGATGATCGCCGGCCGGACATGA

Upstream 100 bases:

>100_bases
AGGTCGCTGCAGAACGGGCGTTCCCTACCCACATCTACTATGAAGCGACAGCGGCGCCCCGCTGTGATGGCTGAGCATGA
CCGACAGAGGCGGGAAGACA

Downstream 100 bases:

>100_bases
ACGCGCACACCTCGGTCGGCCCGCTTGACCGCGCGGCCCGGGTCTACATCGCCGGGCATCGCGGCCTGGTCGGGTCCGCG
CTGCTACGCACGTTTGCGGG

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKRALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARLFLHYGDLIDGTRLVTLLSTIEPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLEAVRLSRVHCRFYQASSSEMFGASPPPQNELTPFYPRSPYGAAKVYS
YWATRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEGMWRMLQTD
EPDDFVLATGRGFTVREFARAAFEHAGLDWQQYVKFDQRYLRPTEVDSLIGDATKAAELLGWRASVHTDELARIMVDADM
AALECEGKPWIDKPMIAGRT

Sequences:

>Translated_340_residues
MKRALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARLFLHYGDLIDGTRLVTLLSTIEPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLEAVRLSRVHCRFYQASSSEMFGASPPPQNELTPFYPRSPYGAAKVYS
YWATRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEGMWRMLQTD
EPDDFVLATGRGFTVREFARAAFEHAGLDWQQYVKFDQRYLRPTEVDSLIGDATKAAELLGWRASVHTDELARIMVDADM
AALECEGKPWIDKPMIAGRT
>Mature_340_residues
MKRALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARLFLHYGDLIDGTRLVTLLSTIEPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLEAVRLSRVHCRFYQASSSEMFGASPPPQNELTPFYPRSPYGAAKVYS
YWATRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEGMWRMLQTD
EPDDFVLATGRGFTVREFARAAFEHAGLDWQQYVKFDQRYLRPTEVDSLIGDATKAAELLGWRASVHTDELARIMVDADM
AALECEGKPWIDKPMIAGRT

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=56.140350877193, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=53.2763532763533, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI48994969, Length=250, Percent_Identity=27.2, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788353, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=56.9767441860465, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=56.9767441860465, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=56.9767441860465, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=56.9767441860465, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=56.9767441860465, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17507723, Length=346, Percent_Identity=56.6473988439306, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=53.6443148688047, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38343; Mature: 38343

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARLFLH
CCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEE
YGDLIDGTRLVTLLSTIEPDEVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLEAVRLSR
ECCCCCHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
VHCRFYQASSSEMFGASPPPQNELTPFYPRSPYGAAKVYSYWATRNYREAYGLFAVNGIL
HHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCEEEEEEEE
FNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEGMWRMLQTD
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHCCC
EPDDFVLATGRGFTVREFARAAFEHAGLDWQQYVKFDQRYLRPTEVDSLIGDATKAAELL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GWRASVHTDELARIMVDADMAALECEGKPWIDKPMIAGRT
HHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARLFLH
CCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEE
YGDLIDGTRLVTLLSTIEPDEVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLEAVRLSR
ECCCCCHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
VHCRFYQASSSEMFGASPPPQNELTPFYPRSPYGAAKVYSYWATRNYREAYGLFAVNGIL
HHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCEEEEEEEE
FNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEGMWRMLQTD
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHCCC
EPDDFVLATGRGFTVREFARAAFEHAGLDWQQYVKFDQRYLRPTEVDSLIGDATKAAELL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GWRASVHTDELARIMVDADMAALECEGKPWIDKPMIAGRT
HHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]