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The map label for this gene is tpiA

Identifier: 148661229

GI number: 148661229

Start: 1616874

End: 1617659

Strand: Direct

Name: tpiA

Synonym: MRA_1446

Alternate gene names: 148661229

Gene position: 1616874-1617659 (Clockwise)

Preceding gene: 148661228

Following gene: 148661231

Centisome position: 36.58

GC content: 64.12

Gene sequence:

>786_bases
GTGAGCCGCAAGCCGCTGATAGCCGGCAACTGGAAGATGAACCTCAACCACTACGAGGCGATCGCGCTGGTGCAAAAGAT
CGCGTTCTCGTTGCCGGACAAGTATTACGACCGGGTTGACGTCGCGGTGATCCCGCCGTTTACCGACCTGCGCAGCGTGC
AAACCCTGGTCGACGGCGACAAGCTGCGGTTGACCTATGGTGCACAAGACTTGTCACCACATGACTCCGGTGCCTATACG
GGTGACGTCAGCGGCGCCTTTCTGGCCAAGTTGGGGTGCAGTTACGTTGTCGTCGGGCACTCCGAGCGGCGCACCTATCA
CAACGAGGATGACGCGCTGGTGGCCGCCAAAGCCGCCACCGCACTCAAGCATGGCTTGACCCCAATCGTGTGTATTGGCG
AGCACCTCGACGTCCGCGAGGCGGGAAATCATGTGGCCCACAACATCGAACAGTTGCGTGGATCGCTGGCCGGGCTATTG
GCCGAGCAGATCGGCAGCGTCGTCATCGCCTACGAACCGGTCTGGGCGATCGGCACCGGGCGGGTGGCCAGCGCCGCCGA
CGCCCAGGAGGTGTGTGCGGCGATCCGAAAAGAGTTGGCCTCGTTGGCCTCGCCGAGGATTGCCGATACGGTGCGGGTGC
TCTACGGCGGCTCGGTGAACGCCAAAAACGTCGGCGACATCGTGGCCCAGGATGACGTCGATGGTGGCCTGGTCGGCGGG
GCGTCGCTGGACGGGGAGCATTTCGCGACGCTGGCCGCGATTGCGGCCGGTGGTCCGTTGCCGTAG

Upstream 100 bases:

>100_bases
ACATATCCACCGGCGGCGGTGCCTCGCTGGAATACCTTGAGGGCAAGACGCTTCCCGGCATCGAGGTACTGAGCCGTGAG
CAGCCAACCGGAGGAGTTTT

Downstream 100 bases:

>100_bases
CGGATCGCGGGCGTGCTACACCCGTAGACCTTCGAGTAGGGCCATAAATGCGCGTTCGACCTCGACTCTGGTCCGGTCTT
TGTCCGTCGCGTCCGCGATC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSRKPLIAGNWKMNLNHYEAIALVQKIAFSLPDKYYDRVDVAVIPPFTDLRSVQTLVDGDKLRLTYGAQDLSPHDSGAYT
GDVSGAFLAKLGCSYVVVGHSERRTYHNEDDALVAAKAATALKHGLTPIVCIGEHLDVREAGNHVAHNIEQLRGSLAGLL
AEQIGSVVIAYEPVWAIGTGRVASAADAQEVCAAIRKELASLASPRIADTVRVLYGGSVNAKNVGDIVAQDDVDGGLVGG
ASLDGEHFATLAAIAAGGPLP

Sequences:

>Translated_261_residues
MSRKPLIAGNWKMNLNHYEAIALVQKIAFSLPDKYYDRVDVAVIPPFTDLRSVQTLVDGDKLRLTYGAQDLSPHDSGAYT
GDVSGAFLAKLGCSYVVVGHSERRTYHNEDDALVAAKAATALKHGLTPIVCIGEHLDVREAGNHVAHNIEQLRGSLAGLL
AEQIGSVVIAYEPVWAIGTGRVASAADAQEVCAAIRKELASLASPRIADTVRVLYGGSVNAKNVGDIVAQDDVDGGLVGG
ASLDGEHFATLAAIAAGGPLP
>Mature_260_residues
SRKPLIAGNWKMNLNHYEAIALVQKIAFSLPDKYYDRVDVAVIPPFTDLRSVQTLVDGDKLRLTYGAQDLSPHDSGAYTG
DVSGAFLAKLGCSYVVVGHSERRTYHNEDDALVAAKAATALKHGLTPIVCIGEHLDVREAGNHVAHNIEQLRGSLAGLLA
EQIGSVVIAYEPVWAIGTGRVASAADAQEVCAAIRKELASLASPRIADTVRVLYGGSVNAKNVGDIVAQDDVDGGLVGGA
SLDGEHFATLAAIAAGGPLP

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=38.7096774193548, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=38.9558232931727, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=42.1686746987952, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=42.6229508196721, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=40.7258064516129, Blast_Score=163, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=39.6761133603239, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=39.6761133603239, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=39.6761133603239, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_MYCBO (P66941)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855125.1
- ProteinModelPortal:   P66941
- EnsemblBacteria:   EBMYCT00000014795
- GeneID:   1092330
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1473
- GeneTree:   EBGT00050000016598
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   MBOV233413:MB1473-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27403; Mature: 27272

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPLIAGNWKMNLNHYEAIALVQKIAFSLPDKYYDRVDVAVIPPFTDLRSVQTLVDGD
CCCCCEEECCEEECCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSPHDSGAYTGDVSGAFLAKLGCSYVVVGHSERRTYHNEDDALVAAKAAT
EEEEEECCCCCCCCCCCCEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHHH
ALKHGLTPIVCIGEHLDVREAGNHVAHNIEQLRGSLAGLLAEQIGSVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCC
RVASAADAQEVCAAIRKELASLASPRIADTVRVLYGGSVNAKNVGDIVAQDDVDGGLVGG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCEEECCCCCCCEECC
ASLDGEHFATLAAIAAGGPLP
CCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRKPLIAGNWKMNLNHYEAIALVQKIAFSLPDKYYDRVDVAVIPPFTDLRSVQTLVDGD
CCCCEEECCEEECCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSPHDSGAYTGDVSGAFLAKLGCSYVVVGHSERRTYHNEDDALVAAKAAT
EEEEEECCCCCCCCCCCCEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHHH
ALKHGLTPIVCIGEHLDVREAGNHVAHNIEQLRGSLAGLLAEQIGSVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCC
RVASAADAQEVCAAIRKELASLASPRIADTVRVLYGGSVNAKNVGDIVAQDDVDGGLVGG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCEEECCCCCCCEECC
ASLDGEHFATLAAIAAGGPLP
CCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972