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The map label for this gene is alkA

Identifier: 148661105

GI number: 148661105

Start: 1478938

End: 1480428

Strand: Reverse

Name: alkA

Synonym: MRA_1325

Alternate gene names: 148661105

Gene position: 1480428-1478938 (Counterclockwise)

Preceding gene: 148661106

Following gene: 148661104

Centisome position: 33.49

GC content: 68.34

Gene sequence:

>1491_bases
GTGCACGACGACTTCGAACGCTGCTACCGGGCGATCCAGTCCAAAGACGCCCGGTTCGACGGCTGGTTCGTCGTCGCGGT
TTTGACCACCGGTGTCTACTGCCGGCCGAGTTGCCCCGTCCGGCCACCGTTCGCGCGCAATGTCCGGTTCCTGCCGACTG
CGGCGGCCGCTCAGGGGGAGGGATTCCGGGCCTGCAAACGGTGCCGCCCCGACGCCTCGCCTGGGTCTCCGGAATGGAAT
GTGCGTAGTGACGTCGTGGCGCGGGCGATGCGGCTGATTGCCGACGGAACGGTGGACCGCGACGGTGTCAGCGGCCTCGC
GGCCCAGCTCGGTTACACCATTCGCCAGCTGGAGCGGCTGTTGCAGGCCGTGGTCGGCGCCGGTCCGCTCGCGTTGGCCC
GCGCCCAACGCATGCAGACCGCCCGGGTGCTGATCGAGACCACGAACCTGCCGTTCGGCGATGTCGCATTCGCCGCCGGG
TTTTCCAGCATCCGTCAGTTCAACGACACCGTTCGCCTGGCGTGCGACGGCACACCGACGGCATTGCGTGCGCGCGCGGC
CGCCCGATTCGAGTCTGCCACCGCATCAGCGGGCACGGTGTCGCTGCGGCTACCCGTCCGTGCACCATTCGCCTTCGAGG
GTGTTTTCGGCCATCTGGCCGCCACCGCGGTGCCGGGTTGCGAAGAGGTCCGCGATGGTGCGTACCGACGCACGCTACGG
CTCCCATGGGGCAACGGCATCGTCAGCCTGACGCCGGCACCCGATCATGTGCGCTGCCTGCTTGTGCTCGATGATTTCCG
CGACCTGATGACGGCCACTGCACGTTGCCGACGGCTGCTGGACCTCGACGCCGATCCCGAAGCGATCGTCGAGGCGCTGG
GCGCCGATCCGGATCTGCGCGCAGTGGTGGGCAAGGCACCCGGGCAACGCATTCCCCGCACAGTCGACGAGGCAGAATTC
GCCGTGCGGGCGGTCCTCGCCCAACAGGTATCGACGAAGGCCGCAAGCACTCACGCGGGCCGACTGGTCGCCGCCTACGG
ACGGCCGGTCCACGATCGCCACGGCGCTTTGACCCACACCTTCCCGTCGATCGAGCAGCTCGCTGAGATCGATCCCGGCC
ATCTGGCCGTCCCCAAGGCGCGTCAAAGGACCATAAACGCGCTCGTCGCCAGCCTTGCCGACAAAAGTCTGGTCCTGGAC
GCCGGATGTGACTGGCAACGCGCCCGCGGGCAGTTGCTAGCGCTGCCCGGAGTGGGCCCCTGGACCGCGGAGGTCATCGC
CATGCGCGGCCTCGGTGACCCGGACGCCTTTCCGGCCAGTGATCTCGGCCTGCGGCTGGCCGCCAAAAAGCTGGGCCTGC
CTGCACAACGACGAGCCCTGACGGTGCACAGCGCTCGCTGGCGCCCCTGGCGCTCCTATGCCACCCAGCACCTGTGGACC
ACCCTGGAACATCCGGTAAACCAATGGCCACCGCAGGAGAAGATCGCATGA

Upstream 100 bases:

>100_bases
TGACCAAGCCGCGTAGGTAACCTGCCCGAACCCACGACGACCCCATCACAATGTCGTTTTTCCGCCAGTCATGTCGGTGG
GCGGGTGTAATTGTTGAAGG

Downstream 100 bases:

>100_bases
TTCACTACCGCACCATCGATAGCCCCATCGGGCCATTAACCCTGGCCGGGCATGGCTCGGTGTTGACGAACCTGCGGATG
CTCGAGCAGACGTATGAGCC

Product: ada regulatory protein AlkA

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA

Sequences:

>Translated_496_residues
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA
>Mature_496_residues
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788383, Length=293, Percent_Identity=32.7645051194539, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=31.9277108433735, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADA_MYCTU (Q10630)

Other databases:

- EMBL:   BX842576
- EMBL:   U65786
- EMBL:   AE000516
- PIR:   A70769
- RefSeq:   NP_215833.1
- RefSeq:   NP_335806.1
- ProteinModelPortal:   Q10630
- SMR:   Q10630
- EnsemblBacteria:   EBMYCT00000003926
- EnsemblBacteria:   EBMYCT00000071713
- GeneID:   886916
- GeneID:   924701
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1358
- KEGG:   mtu:Rv1317c
- TIGR:   MT1358
- TubercuList:   Rv1317c
- GeneTree:   EBGT00050000015628
- HOGENOM:   HBG510250
- OMA:   RCRPDSA
- ProtClustDB:   CLSK791096
- BRENDA:   2.1.1.63
- GO:   GO:0006350
- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294
- Gene3D:   G3DSA:3.40.10.10
- Gene3D:   G3DSA:3.30.310.20
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00278
- SMART:   SM00342

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD; SSF57884 Ada_DNA_repair_Zn-bd; SSF48150 DNA_glycsylse; SSF46689 Homeodomain_like; SSF55945 TFIID_C/glycos_N

EC number: =2.1.1.63

Molecular weight: Translated: 53743; Mature: 53743

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: ACT_SITE 34-34 ACT_SITE 403-403 BINDING 30-30 BINDING 41-41 BINDING 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGE
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCEECCCCCCCCCHHCCEEECCCHHHCCCC
GFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERL
CHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPT
HHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCH
ALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHEEEE
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
CCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHT
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
FPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGP
CCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEECCCCCC
WTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHH
TLEHPVNQWPPQEKIA
HHHHHHHCCCCHHCCC
>Mature Secondary Structure
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGE
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCEECCCCCCCCCHHCCEEECCCHHHCCCC
GFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERL
CHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPT
HHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCH
ALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHEEEE
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
CCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHT
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
FPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGP
CCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEECCCCCC
WTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHH
TLEHPVNQWPPQEKIA
HHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036