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The map label for this gene is emrB [C]

Identifier: 148661037

GI number: 148661037

Start: 1395489

End: 1397228

Strand: Direct

Name: emrB [C]

Synonym: MRA_1258

Alternate gene names: 148661037

Gene position: 1395489-1397228 (Clockwise)

Preceding gene: 148661031

Following gene: 148661040

Centisome position: 31.57

GC content: 64.94

Gene sequence:

>1740_bases
ATGACTACGGCGATACGCCGGGCGGCCGGGAGCAGCTACTTCCGAAACCCCTGGCCTGCGCTGTGGGCGATGATGGTTGG
CTTCTTCATGATCATGCTCGACTCCACCGTCGTAGCCATCGCGAATCCGACCATCATGGCCCAGCTACGCATCGGTTACG
CCACCGTGGTTTGGGTGACCAGCGCCTATCTGCTGGCCTACGCGGTGCCAATGCTGGTGGCCGGCCGGCTTGGCGACCGG
TTCGGCCCGAAGAATCTCTACCTGATTGGCCTGGGGGTATTCACCGTTGCGTCGCTGGGGTGCGGTCTGTCGAGCGGTGC
CGGCATGCTGATTGCCGCTCGAGTGGTGCAAGGCGTCGGCGCCGGATTGCTTACCCCGCAGACGCTGTCGACGATAACGC
GGATCTTCCCGGCTCATCGCCGCGGTGTCGCGCTGGGCGCATGGGGCACCGTCGCCAGTGTCGCCAGCCTGGTGGGACCG
TTGGCCGGCGGCGCGCTGGTCGACAGCATGGGGTGGGAGTGGATTTTCTTCGTCAACGTTCCCGTCGGCGTCATCGGCCT
GATCCTGGCGGCCTATCTGATTCCGGCACTACCCCACCACCCGCATCGGTTCGATTGGTTCGGCGTCGGATTGTCTGGTG
CGGGAATGTTTCTGATTGTCTTCGGACTACAGCAGGGCCAGTCCGCCAATTGGCAGCCTTGGATTTGGGCGGTGATCGTC
GGCGGTATCGGGTTTATGTCGCTGTTCGTTTACTGGCAGGCGCGGAACGCCCGCGAGCCGCTGATCCCACTGGAGGTCTT
CAACGACCGGAACTTCAGCTTGTCCAACCTCAGGATAGCGATCATCGCCTTCGCGGGGACGGGGATGATGCTGCCGGTGA
CGTTTTATGCGCAGGCGGTGTGTGGGTTGTCGCCGACCCACACGGCCGTGCTGTTCGCGCCGACGGCGATCGTCGGTGGC
GTGCTGGCCCCGTTCGTCGGCATGATCATTGACAGGTCCCATCCGTTGTGCGTACTGGGTTTCGGCTTCTCGGTGCTGGC
GATCGCAATGACATGGCTCTTATGCGAGATGGCTCCGGGCACGCCCATCTGGCGGCTGGTGTTGCCGTTCATCGCGTTAG
GCGTTGCTGGGGCGTTCGTGTGGTCGCCGCTGACCGTCACCGCGACCCGCAATCTACGGCCGCACCTGGCCGGTGCGAGC
TCAGGTGTGTTCAACGCCGTCCGGCAGCTGGGGGCTGTGCTGGGGAGCGCGAGCATGGCCGCGTTCATGACGTCGCGCAT
CGCCGCCGAGATGCCCGGTGGTGTGGACGCCCTTACCGGTCCCGCCGGGCAGGACGCTACCGTGTTGCAGCTGCCCGAGT
TCGTGCGCGAACCCTTCGCGGCCGCGATGTCGCAATCGATGCTGTTGCCCGCCTTCGTCGCCCTATTCGGGATCGTTGCC
GCGTTGTTCCTGGTTGACTTCACCGGTGCTGCGGTTGCCAAAGAGCCGTTGCCCGAATCCGATGGCGACGCTGACGACGA
CGACTATGTCGAGTACATCCTTCGTCGGGAACCGGAAGAGGATTGCGACACCCAGCCGCTGCGGGCGTCGCGCCCGGCAG
CGGCCGCAGCGTCACGCAGCGGTGCTGGGGGTCCGCTGGCGGTCAGCTGGTCGACGTCAGCCCAAGGAATGCCCCCAGGT
CCACCAGGCCGTCGGGCGTGGCAGGCAGATACTGAGTCAACAGCTCCGAGCGCACTATAA

Upstream 100 bases:

>100_bases
TAATGCGGCGTGGACATTGGTGACGATACGGGTTGCCCTGGTGCATGCCGTGACGCCCGTGACCCAATGCCACCGCTAGC
AAGCCAAACGAGGTGCGTGT

Downstream 100 bases:

>100_bases
CCGCGGCATACTGTGCCCGACTGACCGCGACGTTGAGCCGATTCCGGTTGAGCAGGAACGAGATTCCGCGTGGAACATCG
TCGGCGGACGAGGCCGTCAT

Product: putative drug-transport integral membrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTTAIRRAAGSSYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVTSAYLLAYAVPMLVAGRLGDR
FGPKNLYLIGLGVFTVASLGCGLSSGAGMLIAARVVQGVGAGLLTPQTLSTITRIFPAHRRGVALGAWGTVASVASLVGP
LAGGALVDSMGWEWIFFVNVPVGVIGLILAAYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIV
GGIGFMSLFVYWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAVCGLSPTHTAVLFAPTAIVGG
VLAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPGTPIWRLVLPFIALGVAGAFVWSPLTVTATRNLRPHLAGAS
SGVFNAVRQLGAVLGSASMAAFMTSRIAAEMPGGVDALTGPAGQDATVLQLPEFVREPFAAAMSQSMLLPAFVALFGIVA
ALFLVDFTGAAVAKEPLPESDGDADDDDYVEYILRREPEEDCDTQPLRASRPAAAAASRSGAGGPLAVSWSTSAQGMPPG
PPGRRAWQADTESTAPSAL

Sequences:

>Translated_579_residues
MTTAIRRAAGSSYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVTSAYLLAYAVPMLVAGRLGDR
FGPKNLYLIGLGVFTVASLGCGLSSGAGMLIAARVVQGVGAGLLTPQTLSTITRIFPAHRRGVALGAWGTVASVASLVGP
LAGGALVDSMGWEWIFFVNVPVGVIGLILAAYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIV
GGIGFMSLFVYWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAVCGLSPTHTAVLFAPTAIVGG
VLAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPGTPIWRLVLPFIALGVAGAFVWSPLTVTATRNLRPHLAGAS
SGVFNAVRQLGAVLGSASMAAFMTSRIAAEMPGGVDALTGPAGQDATVLQLPEFVREPFAAAMSQSMLLPAFVALFGIVA
ALFLVDFTGAAVAKEPLPESDGDADDDDYVEYILRREPEEDCDTQPLRASRPAAAAASRSGAGGPLAVSWSTSAQGMPPG
PPGRRAWQADTESTAPSAL
>Mature_578_residues
TTAIRRAAGSSYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVTSAYLLAYAVPMLVAGRLGDRF
GPKNLYLIGLGVFTVASLGCGLSSGAGMLIAARVVQGVGAGLLTPQTLSTITRIFPAHRRGVALGAWGTVASVASLVGPL
AGGALVDSMGWEWIFFVNVPVGVIGLILAAYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIVG
GIGFMSLFVYWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAVCGLSPTHTAVLFAPTAIVGGV
LAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPGTPIWRLVLPFIALGVAGAFVWSPLTVTATRNLRPHLAGASS
GVFNAVRQLGAVLGSASMAAFMTSRIAAEMPGGVDALTGPAGQDATVLQLPEFVREPFAAAMSQSMLLPAFVALFGIVAA
LFLVDFTGAAVAKEPLPESDGDADDDDYVEYILRREPEEDCDTQPLRASRPAAAAASRSGAGGPLAVSWSTSAQGMPPGP
PGRRAWQADTESTAPSAL

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=29.683698296837, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=29.7169811320755, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=29.5508274231678, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=28.992628992629, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=29.1358024691358, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790146, Length=153, Percent_Identity=26.1437908496732, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=250, Percent_Identity=28.8, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324954, Length=309, Percent_Identity=26.2135922330097, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=240, Percent_Identity=27.5, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319770, Length=264, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323935, Length=299, Percent_Identity=27.0903010033445, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=427, Percent_Identity=22.4824355971897, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=131, Percent_Identity=30.5343511450382, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=27.1356783919598, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=445, Percent_Identity=24.9438202247191, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1250_MYCTU (O50465)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842576
- PIR:   A70954
- RefSeq:   NP_215766.1
- RefSeq:   NP_335733.1
- ProteinModelPortal:   O50465
- EnsemblBacteria:   EBMYCT00000001719
- EnsemblBacteria:   EBMYCT00000072048
- GeneID:   887073
- GeneID:   924781
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1289
- KEGG:   mtu:Rv1250
- TIGR:   MT1289
- TubercuList:   Rv1250
- GeneTree:   EBGT00050000014781
- HOGENOM:   HBG679341
- OMA:   FIPREHF
- ProtClustDB:   CLSK791048
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 60909; Mature: 60778

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1caebcd4)-; HASH(0x1dabe65c)-; HASH(0x1df55b88)-; HASH(0x1ddf3068)-; HASH(0x1d75159c)-; HASH(0x1c2abd54)-; HASH(0x1df901f4)-; HASH(0x1daca474)-; HASH(0x1df85230)-; HASH(0x1d4f1000)-; HASH(0x1df850f8)-; HASH(0x1dd98404)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAIRRAAGSSYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCHHHHHHHH
SAYLLAYAVPMLVAGRLGDRFGPKNLYLIGLGVFTVASLGCGLSSGAGMLIAARVVQGVG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
AGLLTPQTLSTITRIFPAHRRGVALGAWGTVASVASLVGPLAGGALVDSMGWEWIFFVNV
CCCCCHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEC
PVGVIGLILAAYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIV
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
GGIGFMSLFVYWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAV
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCEEEEEEEEECCCCCHHHHHHHHHH
CGLSPTHTAVLFAPTAIVGGVLAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPG
HCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCC
TPIWRLVLPFIALGVAGAFVWSPLTVTATRNLRPHLAGASSGVFNAVRQLGAVLGSASMA
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFMTSRIAAEMPGGVDALTGPAGQDATVLQLPEFVREPFAAAMSQSMLLPAFVALFGIVA
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLVDFTGAAVAKEPLPESDGDADDDDYVEYILRREPEEDCDTQPLRASRPAAAAASRS
HHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
GAGGPLAVSWSTSAQGMPPGPPGRRAWQADTESTAPSAL
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
TTAIRRAAGSSYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVT
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCHHHHHHHH
SAYLLAYAVPMLVAGRLGDRFGPKNLYLIGLGVFTVASLGCGLSSGAGMLIAARVVQGVG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
AGLLTPQTLSTITRIFPAHRRGVALGAWGTVASVASLVGPLAGGALVDSMGWEWIFFVNV
CCCCCHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEC
PVGVIGLILAAYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIV
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
GGIGFMSLFVYWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAV
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCEEEEEEEEECCCCCHHHHHHHHHH
CGLSPTHTAVLFAPTAIVGGVLAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPG
HCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCC
TPIWRLVLPFIALGVAGAFVWSPLTVTATRNLRPHLAGASSGVFNAVRQLGAVLGSASMA
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFMTSRIAAEMPGGVDALTGPAGQDATVLQLPEFVREPFAAAMSQSMLLPAFVALFGIVA
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLVDFTGAAVAKEPLPESDGDADDDDYVEYILRREPEEDCDTQPLRASRPAAAAASRS
HHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
GAGGPLAVSWSTSAQGMPPGPPGRRAWQADTESTAPSAL
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036