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The map label for this gene is narI [H]

Identifier: 148660952

GI number: 148660952

Start: 1294716

End: 1295456

Strand: Direct

Name: narI [H]

Synonym: MRA_1175

Alternate gene names: 148660952

Gene position: 1294716-1295456 (Clockwise)

Preceding gene: 148660951

Following gene: 148660953

Centisome position: 29.29

GC content: 63.16

Gene sequence:

>741_bases
TTGGCCGTCTTGGACTTGGTTGAGATCTTCTGGGATGCCGCGCCTTACGTCGTTGTGGCGATCGCGGTGGTCGGCACCTG
GTGGCGGTATCGCTACGACAAGTTCGGCTGGACCACACGCTCGTCGCAGCTCTACGAGTCGCGGTTGCTGTCGATCGGCA
GCCCGATGTTCCATTTCGGCAGCTTGCTGGTGATCATGGGCCACGTGATGGGCCTGTTCATTCCGGATTCCTGGACCAGA
GCGTTCGGCATGAGCGATCACCTGTACCATCTGCAGGCGCTGCTGCTTGGCGCGCCCGCCGGTTTCGCCACTCTGCTCGG
TATCGGGTTGCTGATCTATCGGCGGCGCATCCAGACACCGGTGTGGCTGGCTACCACTCGGAATGACAAGCTGATGTACC
TGGTGCTGGTGTGCGCGATCGTGGCTGGCCTGGCATGCACGCTGATGGGCGCCACCCATGAGGGCGATATGCACGATTAC
CGGCGCTCGGTGTCGGTCTGGTTCCGCTCGATCTGGATGCTAGCGCCGCGTGGCGATCTGATGGCCCAGGCGACGCTGTA
CTACCAGGTGCATGTGCTGATCGCGCTCGCGCTGTTTGCGCTCTGGCCGTTTACCCGATTGGTGCACGCGTTCAGCGCGC
CGATCGCCTACCTGTTCCGGCCCTACATCGTGTACCGCAGCCGCGAGGTGGCGGCCAAGCACGAATTGATCGGTTCCGCG
CCGCGTCGTCGTGGGTGGTAG

Upstream 100 bases:

>100_bases
GGGCACAACGCCTAGCTCAGTCGGGGCCGCCCGCGGAAGCCGTTGGTTTGCAACCGTTTACCTTGACCGTCCCGCCCAAG
CGCGCCGAGGGGGCCTGACC

Downstream 100 bases:

>100_bases
TTCTCTGCCACAATCACCGTCGTGCCATTCCGCAACGTTGCCATCGTCGCGCACGTCGACCACGGCAAGACCACCCTGGT
TGACGCCATGTTGCGGCAGT

Product: putative respiratory nitrate reductase gamma chain NarI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVLDLVEIFWDAAPYVVVAIAVVGTWWRYRYDKFGWTTRSSQLYESRLLSIGSPMFHFGSLLVIMGHVMGLFIPDSWTR
AFGMSDHLYHLQALLLGAPAGFATLLGIGLLIYRRRIQTPVWLATTRNDKLMYLVLVCAIVAGLACTLMGATHEGDMHDY
RRSVSVWFRSIWMLAPRGDLMAQATLYYQVHVLIALALFALWPFTRLVHAFSAPIAYLFRPYIVYRSREVAAKHELIGSA
PRRRGW

Sequences:

>Translated_246_residues
MAVLDLVEIFWDAAPYVVVAIAVVGTWWRYRYDKFGWTTRSSQLYESRLLSIGSPMFHFGSLLVIMGHVMGLFIPDSWTR
AFGMSDHLYHLQALLLGAPAGFATLLGIGLLIYRRRIQTPVWLATTRNDKLMYLVLVCAIVAGLACTLMGATHEGDMHDY
RRSVSVWFRSIWMLAPRGDLMAQATLYYQVHVLIALALFALWPFTRLVHAFSAPIAYLFRPYIVYRSREVAAKHELIGSA
PRRRGW
>Mature_245_residues
AVLDLVEIFWDAAPYVVVAIAVVGTWWRYRYDKFGWTTRSSQLYESRLLSIGSPMFHFGSLLVIMGHVMGLFIPDSWTRA
FGMSDHLYHLQALLLGAPAGFATLLGIGLLIYRRRIQTPVWLATTRNDKLMYLVLVCAIVAGLACTLMGATHEGDMHDYR
RSVSVWFRSIWMLAPRGDLMAQATLYYQVHVLIALALFALWPFTRLVHAFSAPIAYLFRPYIVYRSREVAAKHELIGSAP
RRRGW

Specific function: Does not seem to have nitrate reductase activity [H]

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the nitrate reductase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787738, Length=223, Percent_Identity=31.390134529148, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787480, Length=222, Percent_Identity=29.7297297297297, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020945
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR003816
- InterPro:   IPR003765 [H]

Pfam domain/function: PF00384 Molybdopterin; PF02613 Nitrate_red_del; PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 28058; Mature: 27927

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLDLVEIFWDAAPYVVVAIAVVGTWWRYRYDKFGWTTRSSQLYESRLLSIGSPMFHFG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHH
SLLVIMGHVMGLFIPDSWTRAFGMSDHLYHLQALLLGAPAGFATLLGIGLLIYRRRIQTP
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VWLATTRNDKLMYLVLVCAIVAGLACTLMGATHEGDMHDYRRSVSVWFRSIWMLAPRGDL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
MAQATLYYQVHVLIALALFALWPFTRLVHAFSAPIAYLFRPYIVYRSREVAAKHELIGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
PRRRGW
CCCCCC
>Mature Secondary Structure 
AVLDLVEIFWDAAPYVVVAIAVVGTWWRYRYDKFGWTTRSSQLYESRLLSIGSPMFHFG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHH
SLLVIMGHVMGLFIPDSWTRAFGMSDHLYHLQALLLGAPAGFATLLGIGLLIYRRRIQTP
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VWLATTRNDKLMYLVLVCAIVAGLACTLMGATHEGDMHDYRRSVSVWFRSIWMLAPRGDL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
MAQATLYYQVHVLIALALFALWPFTRLVHAFSAPIAYLFRPYIVYRSREVAAKHELIGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
PRRRGW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]