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The map label for this gene is narH [H]

Identifier: 148660950

GI number: 148660950

Start: 1292375

End: 1294051

Strand: Direct

Name: narH [H]

Synonym: MRA_1173

Alternate gene names: 148660950

Gene position: 1292375-1294051 (Clockwise)

Preceding gene: 148660949

Following gene: 148660951

Centisome position: 29.24

GC content: 64.88

Gene sequence:

>1677_bases
ATGAAGGTCATGGCGCAGATGGCGATGGTGATGAACCTCGACAAATGCATTGGTTGCCATACCTGCTCGGTGACCTGCAA
GCAGGCCTGGACCAATCGCTCGGGAACCGAGTACGTGTGGTTCAACAATGTCGAAACCCGTCCGGGTGTGGGCTACCCGC
GCACCTACGAGGATCAGGAGCGGTGGCGCGGGGGGTGGGTGCGCGACAAGAAGGGCCGGCTGCGGCTGCGCGACGGCGGC
CGGATCCATAAGCTGTTGCGCATCTTTGCCAACCCCAAGCTGCCCACTATCGGCGACTACTACGAGCCGTGGACCTATGA
CTACGAAAACCTGACATCGGCGCCGGCGGGTGACACCTTTCCGACCGCGGCGCCGCGAAGCCTGATCAGCGGCAATCCGA
TGAAGGTGTCGTGGGGATCCAACTGGGACGACAACCTGGCCGGGTCGCCAGAGATCGTGCCGAACGACCCGGTGCTAAAG
AAGGTCAACCAAGTCAACCAAGAGGTCAAGCTGAAGCTTGAAGAGACCTTCATGTTTTACCTGCCGCGGATCTGCGAGCA
CTGCCTGAACCCGTCGTGTGTGGCGTCGTGTCCGTCGGGGGCGATGTACAAGCGCACCGAGGACGGCATCGTGCTCGTCG
ACCAGGACCGCTGCCGCGGCTGGCGGATGTGTGTGTCCGGGTGCCCATACAAGAAGGTGTATTTCAACCACAAGACCGGC
AAGGCCGAAAAGTGCACCCTGTGCTATCCGCGCATCGAGGTGGGGTTGCCGACGGTGTGCTCGGAAACGTGTGTGGGGCG
GCTGCGCTATCTGGGTCTGGTGCTCTATGACGTCGATCAGGTGCTGCAGGCCGCGTCGGTGGAAAGCGACACCGACCTCT
ACGAGGCGCAGCGCCGGATCCTGCTGGACCCGCACGATCCGCGGGTGATCGCCGGGGCGCGCGCGGAAGGCATCGCCGAC
GAGTGGATCGAGGCCGCCCAGCGGTCCCCGGTGTACGCGTTGATCAACACCTACCGGGTGGCGCTGCCGCTACATCCAGA
GTACCGGACCATGCCGATGGTCTGGTACATCCCGCCGCTGTCGCCGGTGGTCGACGCGGTCAGCCGCGACGGGCACGACG
GGGAGGACCTGGGCAATTTGTTCGGCGCGCTGGACGCACTGCGGATTCCGATTGCCTATCTGGCCGAGCTGTTCACCGCG
GGCGACACCGAGGTGGTCGCGGGCGTGTTGCGGCGGCTGGCGGCGATGCGCTGCTACATGCGCGACATCAACCTGGGCCG
GGAGACCCAGCCCCACATCCCGGAATCGGTCGGGATGACCGAGGAGCAGATCTACCAGATGTACCGACTGTTGGCTGTGG
CGAAATATGAAGAGCGCTATGTCATTCCGACGTCGTACGCGGGGGAGCTGCCGGCCGCGGCGATGACCGACGATATGGGG
TGCTCGTTGTCGGTCGACGGCGGACCGGGAATGTACGAGTCCGGTCCGTTCGGGCAGGGCAGCCCTACTCCGGTGCCAAT
CGCCGTGGAGAGCTTCCACGCTCTGCAGCATGCCGGTAGCGCGGCCACCGGCGGCGCTGGCCGATCCCGGGTCAACCTGC
TCAACTGGGACCCCAACGGCGCAGCGGCGGGGCTCTTCCCGGAGCCTCAGCCCAGCAAGGATGTGGTCCAGCGATGA

Upstream 100 bases:

>100_bases
CCGGTAACCAGCGTGACGAGGTGACCGTGGTGCGCCGCCGCAGCCAGGAAGTGCGGTACTGACCAATGAAGGGCCCGAGC
GACGCTTGCGGAGCGAGACG

Downstream 100 bases:

>100_bases
AGTTGCTGTCTCGTGTCCGAGAGCGGTCGAGCGCCACCACAATGAGGGACCGACTGGTGTGGCAGTCGGCCTCGCTACTG
CTGGCCTATCCGGATGACGG

Product: putative respiratory nitrate reductase subunit beta NarH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQERWRGGWVRDKKGRLRLRDGG
RIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLK
KVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRILLDPHDPRVIAGARAEGIAD
EWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTA
GDTEVVAGVLRRLAAMRCYMRDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNGAAAGLFPEPQPSKDVVQR

Sequences:

>Translated_558_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQERWRGGWVRDKKGRLRLRDGG
RIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLK
KVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRILLDPHDPRVIAGARAEGIAD
EWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTA
GDTEVVAGVLRRLAAMRCYMRDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNGAAAGLFPEPQPSKDVVQR
>Mature_558_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQERWRGGWVRDKKGRLRLRDGG
RIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLK
KVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRILLDPHDPRVIAGARAEGIAD
EWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTA
GDTEVVAGVLRRLAAMRCYMRDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNGAAAGLFPEPQPSKDVVQR

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=489, Percent_Identity=56.0327198364008, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1787478, Length=492, Percent_Identity=54.4715447154472, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=42.5742574257426, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=42.5742574257426, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789370, Length=145, Percent_Identity=33.7931034482759, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI226510944, Length=91, Percent_Identity=37.3626373626374, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=36.25, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=80, Percent_Identity=35, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367345, Length=86, Percent_Identity=33.7209302325581, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 62038; Mature: 62038

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQE
CCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTF
HHCCCCEECCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
PTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLKKVNQVNQEVKLKLEETFMFY
CCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHEEHHHHHHHHH
LPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
HHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCC
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRI
CCCEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHE
LLDPHDPRVIAGARAEGIADEWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPL
ECCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCEEEEECCH
SPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTAGDTEVVAGVLRRLAAMRCYM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
HHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHCCCCC
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNG
CEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
AAAGLFPEPQPSKDVVQR
CCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQE
CCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTF
HHCCCCEECCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
PTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLKKVNQVNQEVKLKLEETFMFY
CCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHEEHHHHHHHHH
LPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
HHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCC
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRI
CCCEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHE
LLDPHDPRVIAGARAEGIADEWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPL
ECCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCEEEEECCH
SPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTAGDTEVVAGVLRRLAAMRCYM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
HHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHCCCCC
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNG
CEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
AAAGLFPEPQPSKDVVQR
CCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]