Click here to switch to the map view.

The map label for this gene is pimE

Identifier: 148660946

GI number: 148660946

Start: 1286302

End: 1287597

Strand: Direct

Name: pimE

Synonym: MRA_1169

Alternate gene names: 148660946

Gene position: 1286302-1287597 (Clockwise)

Preceding gene: 148660943

Following gene: 148660948

Centisome position: 29.1

GC content: 65.05

Gene sequence:

>1296_bases
ATGTGCCGCACCCTGATCGACGGCCCGGTGCGCTCGGCGATCGCTAAAGTCAGGCAGATAGACACCACCTCATCCACCCC
GGCGGCCGCCAGGCGCGTGACCTCACCACCGGCCCGGGAGACACGCGCCGCCGTGCTGCTACTGGTCCTCAGCGTCGGTG
CGCGACTCGCCTGGACCTATCTGGCGCCCAACGGCGCAAACTTCGTCGACCTGCACGTTTACGTGAGCGGTGCAGCGTCC
CTCGACCATCCCGGCACCCTGTATGGCTACGTCTACGCTGATCAGACCCCGGACTTCCCGCTGCCGTTCACCTATCCGCC
GTTTGCGGCTGTGGTCTTCTACCCGTTGCATTTGGTGCCGTTCGGTCTGATCGCGCTGCTGTGGCAAGTAGTGACGATGG
CCGCGCTCTACGGCGCGGTTCGGATCAGCCAGCGCCTGATGGGGGGCACCGCTGAGACCGGTCATTTCGCCGCGATGTTA
TGGACGGCGATCGCCATCTGGATCGAGCCGTTGCGCAGCACCTTTGACTATGGGCAGATCAACGTGCTGCTGATGCTGGC
GGCGCTTTGGGCGGTCTACACCCCGCGGTGGTGGCTATCGGGACTGCTGGTCGGGGTGGCCTCGGGTGTCAAGTTGACGC
CGGCGATTACCGCTGTCTACCTCGTCGGCGTTCGGCGGTTGCATGCGGCCGCATTTTCGGTGGTCGTGTTCCTTGCCACC
GTCGGCGTGTCGCTACTGGTCGTCGGCGATGAAGCCCGCTACTACTTCACCGACCTGTTGGGCGACGCAGGCCGGGTTGG
GCCCATCGCCACCTCCTTCAATCAATCCTGGCGCGGCGCGATTTCCCGGATTCTCGGTCACGACGCCGGTTTTGGTCCGC
TGGTTCTGGCTGCGATCGCCAGTACGGCGGTATTGGCCATCCTGGCCTGGCGTGCGCTCGACAGGTCCGATCGGCTGGGC
AAACTATTGGTGGTCGAGTTGTTCGGCCTGCTGCTCTCGCCGATCTCCTGGACTCACCACTGGGTGTGGCTAGTGCCGCT
GATGATCTGGCTGATTGACGGGCCAGCGCGTGAGCGCCCGGGCGCCCGGATTTTGGGCTGGGGCTGGTTGGTGTTGACCA
TCGTCGGCGTGCCGTGGTTGCTGAGCTTTGCTCAACCGAGCATCTGGCAAATCGGCCGGCCGTGGTATTTGGCCTGGGCC
GGTCTGGTCTACGTGGTGGCGACGCTGGCGACCTTGGGCTGGATCGCCGCCTCCGAGCGTTACGTGCGCATTCGGCCGCG
GCGCATGGCCAATTAG

Upstream 100 bases:

>100_bases
CGCCGAACGTATCGCGGTGCAGATGTGACTCAAGTGACACGTGTGGCATTTATGTGATTGTTACGGATACGAGTGGTTGT
GGTGACCGGGCACCCGAGTG

Downstream 100 bases:

>100_bases
GCCCCAAACATTGCGTCGATATCGTGCGCCATCGCAATGTCGTTTTCCGTGATACCACCTACCGCATGCGTAACCAGCGC
GAAAGTTACTGTTCGCCAAC

Product: mannosyltransferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Alpha-manT; PPM-dependent mannosyltransferase; Polyprenol-phosphate-mannose alpha-mannosyltransferase; PPM alpha-mannosyltransferase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTYLAPNGANFVDLHVYVSGAAS
LDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVPFGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAML
WTAIAIWIEPLRSTFDYGQINVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLAT
VGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIASTAVLAILAWRALDRSDRLG
KLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERPGARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWA
GLVYVVATLATLGWIAASERYVRIRPRRMAN

Sequences:

>Translated_431_residues
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTYLAPNGANFVDLHVYVSGAAS
LDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVPFGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAML
WTAIAIWIEPLRSTFDYGQINVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLAT
VGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIASTAVLAILAWRALDRSDRLG
KLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERPGARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWA
GLVYVVATLATLGWIAASERYVRIRPRRMAN
>Mature_431_residues
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTYLAPNGANFVDLHVYVSGAAS
LDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVPFGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAML
WTAIAIWIEPLRSTFDYGQINVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLAT
VGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIASTAVLAILAWRALDRSDRLG
KLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERPGARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWA
GLVYVVATLATLGWIAASERYVRIRPRRMAN

Specific function: Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGM family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIME_MYCTU (O06557)

Other databases:

- EMBL:   BX842575
- EMBL:   AE000516
- PIR:   H70555
- RefSeq:   NP_215675.1
- RefSeq:   NP_335638.1
- ProteinModelPortal:   O06557
- EnsemblBacteria:   EBMYCT00000000066
- EnsemblBacteria:   EBMYCT00000071606
- GeneID:   885899
- GeneID:   924883
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1195
- KEGG:   mtu:Rv1159
- TIGR:   MT1195
- TubercuList:   Rv1159
- GeneTree:   EBGT00050000017048
- HOGENOM:   HBG564831
- OMA:   TRWWLSG
- ProtClustDB:   PRK13375
- GO:   GO:0009405
- InterPro:   IPR018584

Pfam domain/function: PF09594 DUF2029

EC number: NA

Molecular weight: Translated: 47103; Mature: 47103

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d18c608)-; HASH(0x1dc90978)-; HASH(0x1da2aa78)-; HASH(0x1dba9634)-; HASH(0x1d9244e4)-; HASH(0x1d84c1b8)-; HASH(0x1c72e74c)-; HASH(0x1dd1c37c)-; HASH(0x1d4b70bc)-; HASH(0x1dbef470)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTY
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEE
LAPNGANFVDLHVYVSGAASLDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVP
ECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
FGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAMLWTAIAIWIEPLRSTFDYGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
NVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLAT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIA
HCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
STAVLAILAWRALDRSDRLGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWAGLVYVVATLATLGWIAASER
CCEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YVRIRPRRMAN
EEEECCHHCCH
>Mature Secondary Structure
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTY
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEE
LAPNGANFVDLHVYVSGAASLDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVP
ECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
FGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAMLWTAIAIWIEPLRSTFDYGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
NVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLAT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIA
HCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
STAVLAILAWRALDRSDRLGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWAGLVYVVATLATLGWIAASER
CCEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YVRIRPRRMAN
EEEECCHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036