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The map label for this gene is ppdK [H]

Identifier: 148660913

GI number: 148660913

Start: 1251242

End: 1252714

Strand: Reverse

Name: ppdK [H]

Synonym: MRA_1136

Alternate gene names: 148660913

Gene position: 1252714-1251242 (Counterclockwise)

Preceding gene: 148660914

Following gene: 148660912

Centisome position: 28.34

GC content: 66.53

Gene sequence:

>1473_bases
ATGACTCGTATCACCCGAGCCAATGGTTGCCCCGACGGAACTCTCGAAAACGCCGTTGTGGCGCTGGACGGCGGCGCGAA
CTACCCACGCGAGATCCTGGGCAACAAAGGCCACGGCATCGACATGATGCGCCGGCACCATCTGCCGGTGCCGCCTGCAT
TCTGCATTACCACCGAGGTGGGCGTGCGCTATCTCGCCGCGCCCGGATCCACGATCGCCGCGATCTGGGATGACGTGCTG
GATCGGATGAGCTGGCTGGAAACAGAAACCTCGTGCACGTTCGGCCGTGGCCCGAATCCGCTGCTGGTCAGCGTGCGCTC
GGGAGCCACCCAGTCGATGCCCGGCATGATGGACACGATTCTGGACGTGGGCATGACCGACGCGGTCGAACGAGTACTTG
CCAGGCCGGGTGCCGCCGACTTCGCCCACGACACCAGACGGCGATTCACCAGCATGTATCGGCGCATCGTCGGCAGCGCC
GGTCCCATTACCGACGACCCGTATGCCCAGTTGCGCGCGAGCATCGAGGCGGTATTCGCCTCCTGGAACTCACCTCGCGC
CGTTGCCTATCGCGACCATCACGGCCTCGACGACCAAGGCGGTACGGCGGTGGTGGTGCAGGCGATGGTATTCGGCAACC
TCACCGCGAACTCGGGCGCCGGGGTGCTGTCCTCACGCAACCCGATCACCGGAGCCAACGAACCGTTCGGCGAATGGCTA
CCCGGCGGCCAAGGCGACGACGTGGTGTCCGGATTGGTCGCCGTCGCACCGATCACCGCCCTGCGCGACCAGCAGCCGGC
CGTCTACGACCAACTGATGGCGGCCGCCCGCAGTTTGGAGCGCATGGCAGGCGACGTCCAGGAGATCGAATTCACCGTGG
AGGACAGCCAGCTGTGGCTGCTGCAGACCCGCGGGGCGGAACGCTCGGCGCAGGCCGCCGTGCGACTGGCGCTGCAACTG
CATCACGAGGGACTCATCGACGACACCGAGACACTGCGCCGGGTGACTCCGACGCACATCGAGACTTTGCTACGGCCGTC
GCTGCAGACGGAAACACGGTTGGCTGCACCGCTTTTGGCCAAGGGCCTGCCCGCTTGCCCGGGCGTGGTATCCGGGACCG
CCTACACCGAGGTGGACGAGGCGCTGGACGCTGCGGACCGGGGCGAGCCGGTCATCTTGGTGCGAGATCACACCAGACCG
GAGGACGTCATGGGCATGCTTGCCGCGCAAGGCATTGTCACCGAGGTGGGGGGTGCCGCCAGTCATGCGGCGGTGGTTAG
CCGCGAGCTCGGTCGAGTGGCCGTGGTGGGTTGCGGTCCCGGGGTCGCGGCGGCACTGGCCGGCAAGGAGATCACCGTCG
ACGGCTATGAAGGCGAAGTGCGCCAAGGCGTTCTGGCATTGTCTGCCTGGTCGGAAAGTGACACGCCGGAGTTGCGCGAG
CTGGCCGACATCGCGCAACGGATCAGTTCATGA

Upstream 100 bases:

>100_bases
CCAGACACAAGCTGAGTAGTCCGACCGCTGACCAAGGCGCCCCCTGTGGGCCCACAGTGGGCGGTCTGGCGTTGCCCATA
AGTAGCATCAAGGGGTGCCC

Downstream 100 bases:

>100_bases
GCGAGTTGACGGTACTGCAGGCCGTGCGGCTCAAGGGCCGGGTGATCACCACCGACCTGGCGCAGACCTTGGGTGAAGAC
CTTGCCGACGTCGCCGCGAC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTRITRANGCPDGTLENAVVALDGGANYPREILGNKGHGIDMMRRHHLPVPPAFCITTEVGVRYLAAPGSTIAAIWDDVL
DRMSWLETETSCTFGRGPNPLLVSVRSGATQSMPGMMDTILDVGMTDAVERVLARPGAADFAHDTRRRFTSMYRRIVGSA
GPITDDPYAQLRASIEAVFASWNSPRAVAYRDHHGLDDQGGTAVVVQAMVFGNLTANSGAGVLSSRNPITGANEPFGEWL
PGGQGDDVVSGLVAVAPITALRDQQPAVYDQLMAAARSLERMAGDVQEIEFTVEDSQLWLLQTRGAERSAQAAVRLALQL
HHEGLIDDTETLRRVTPTHIETLLRPSLQTETRLAAPLLAKGLPACPGVVSGTAYTEVDEALDAADRGEPVILVRDHTRP
EDVMGMLAAQGIVTEVGGAASHAAVVSRELGRVAVVGCGPGVAAALAGKEITVDGYEGEVRQGVLALSAWSESDTPELRE
LADIAQRISS

Sequences:

>Translated_490_residues
MTRITRANGCPDGTLENAVVALDGGANYPREILGNKGHGIDMMRRHHLPVPPAFCITTEVGVRYLAAPGSTIAAIWDDVL
DRMSWLETETSCTFGRGPNPLLVSVRSGATQSMPGMMDTILDVGMTDAVERVLARPGAADFAHDTRRRFTSMYRRIVGSA
GPITDDPYAQLRASIEAVFASWNSPRAVAYRDHHGLDDQGGTAVVVQAMVFGNLTANSGAGVLSSRNPITGANEPFGEWL
PGGQGDDVVSGLVAVAPITALRDQQPAVYDQLMAAARSLERMAGDVQEIEFTVEDSQLWLLQTRGAERSAQAAVRLALQL
HHEGLIDDTETLRRVTPTHIETLLRPSLQTETRLAAPLLAKGLPACPGVVSGTAYTEVDEALDAADRGEPVILVRDHTRP
EDVMGMLAAQGIVTEVGGAASHAAVVSRELGRVAVVGCGPGVAAALAGKEITVDGYEGEVRQGVLALSAWSESDTPELRE
LADIAQRISS
>Mature_489_residues
TRITRANGCPDGTLENAVVALDGGANYPREILGNKGHGIDMMRRHHLPVPPAFCITTEVGVRYLAAPGSTIAAIWDDVLD
RMSWLETETSCTFGRGPNPLLVSVRSGATQSMPGMMDTILDVGMTDAVERVLARPGAADFAHDTRRRFTSMYRRIVGSAG
PITDDPYAQLRASIEAVFASWNSPRAVAYRDHHGLDDQGGTAVVVQAMVFGNLTANSGAGVLSSRNPITGANEPFGEWLP
GGQGDDVVSGLVAVAPITALRDQQPAVYDQLMAAARSLERMAGDVQEIEFTVEDSQLWLLQTRGAERSAQAAVRLALQLH
HEGLIDDTETLRRVTPTHIETLLRPSLQTETRLAAPLLAKGLPACPGVVSGTAYTEVDEALDAADRGEPVILVRDHTRPE
DVMGMLAAQGIVTEVGGAASHAAVVSRELGRVAVVGCGPGVAAALAGKEITVDGYEGEVRQGVLALSAWSESDTPELREL
ADIAQRISS

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=329, Percent_Identity=27.0516717325228, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 52005; Mature: 51874

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRITRANGCPDGTLENAVVALDGGANYPREILGNKGHGIDMMRRHHLPVPPAFCITTEV
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHCCCCCCCHHEEEHHH
GVRYLAAPGSTIAAIWDDVLDRMSWLETETSCTFGRGPNPLLVSVRSGATQSMPGMMDTI
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHH
LDVGMTDAVERVLARPGAADFAHDTRRRFTSMYRRIVGSAGPITDDPYAQLRASIEAVFA
HHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SWNSPRAVAYRDHHGLDDQGGTAVVVQAMVFGNLTANSGAGVLSSRNPITGANEPFGEWL
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
PGGQGDDVVSGLVAVAPITALRDQQPAVYDQLMAAARSLERMAGDVQEIEFTVEDSQLWL
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEEEECCEEEE
LQTRGAERSAQAAVRLALQLHHEGLIDDTETLRRVTPTHIETLLRPSLQTETRLAAPLLA
EEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHH
KGLPACPGVVSGTAYTEVDEALDAADRGEPVILVRDHTRPEDVMGMLAAQGIVTEVGGAA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCH
SHAAVVSRELGRVAVVGCGPGVAAALAGKEITVDGYEGEVRQGVLALSAWSESDTPELRE
HHHHHHHHHCCCEEEEECCCCHHHHHCCCEEEEECCCCHHHHCEEEEEECCCCCCHHHHH
LADIAQRISS
HHHHHHHHCC
>Mature Secondary Structure 
TRITRANGCPDGTLENAVVALDGGANYPREILGNKGHGIDMMRRHHLPVPPAFCITTEV
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHCCCCCCCHHEEEHHH
GVRYLAAPGSTIAAIWDDVLDRMSWLETETSCTFGRGPNPLLVSVRSGATQSMPGMMDTI
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHH
LDVGMTDAVERVLARPGAADFAHDTRRRFTSMYRRIVGSAGPITDDPYAQLRASIEAVFA
HHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SWNSPRAVAYRDHHGLDDQGGTAVVVQAMVFGNLTANSGAGVLSSRNPITGANEPFGEWL
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
PGGQGDDVVSGLVAVAPITALRDQQPAVYDQLMAAARSLERMAGDVQEIEFTVEDSQLWL
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEEEECCEEEE
LQTRGAERSAQAAVRLALQLHHEGLIDDTETLRRVTPTHIETLLRPSLQTETRLAAPLLA
EEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHH
KGLPACPGVVSGTAYTEVDEALDAADRGEPVILVRDHTRPEDVMGMLAAQGIVTEVGGAA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCH
SHAAVVSRELGRVAVVGCGPGVAAALAGKEITVDGYEGEVRQGVLALSAWSESDTPELRE
HHHHHHHHHCCCEEEEECCCCHHHHHCCCEEEEECCCCHHHHCEEEEEECCCCCCHHHHH
LADIAQRISS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]