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The map label for this gene is engD [H]

Identifier: 148660898

GI number: 148660898

Start: 1239565

End: 1240638

Strand: Direct

Name: engD [H]

Synonym: MRA_1123

Alternate gene names: 148660898

Gene position: 1239565-1240638 (Clockwise)

Preceding gene: 148660896

Following gene: 148660899

Centisome position: 28.04

GC content: 65.74

Gene sequence:

>1074_bases
GTGAGCTTGAGCCTGGGGATCGTGGGCCTGCCCAACGTCGGCAAGTCGACACTTTTCAACGCGCTGACCCGAAACAACGT
GGTCGCGGCCAACTACCCGTTCGCGACGATCGAACCGAACGAAGGTGTCGTCTCCCTGCCCGATCCCCGCCTGGACAAGC
TTGCTGAGCTTTTCGGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCG
TCCGAGGGAGCCGGGCTGGGTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTT
CGTCGACGACGACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCGAGCTGATCC
TGGCAGATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAAGGAAGCGCGCACCAACAAGGCGCGCAAGCCGGTC
TACGACGCGGCACTGCGTGCCCAGCAGGTGCTCGACGCCGGCAAGACGCTGTTCGCCGCGGGGGTGGATGCCGCCGCGTT
GCGCGAGCTGAACCTGCTGACCACCAAGCCCTTCCTGTATGTGTTCAACGCCGACGAGGCGGTGCTCACCGACCCGGCGC
GAGTCGGTGAGCTGCGCGCGTTGGTGGCGCCCGCCGATGCGGTGTTCCTGGACGCCGCCATCGAGTCGGAGTTGACCGAA
CTGGACGACGAGTCGGCCGCGGAGCTGCTGGAGTCCATCGGGCAGAGCGAGCGCGGGCTGGACGCGCTGGCCCGGGCGGG
TTTTCACACCCTGAAGTTGCAGACCTTTTTGACCGCGGGCCCCAAGGAAGCGCGGGCGTGGACCATCCATCAAGGCGACA
CCGCGCCGAAGGCGGCCGGGGTGATCCACAGCGACTTCGAGAAGGGTTTCATCAAGGCCGAGATCGTGTCCTACGACGAC
CTGGTGGCCGCGGGTTCGATGGCGGCGGCCAAGGCGGCCGGCAAGGTCCGGATCGAAGGCAAGGACTACGTGATGGCCGA
CGGTGACGTAGTGGAGTTCCGATTCAACGTGTAG

Upstream 100 bases:

>100_bases
CTTGCCTGTACCGCCGACCTCGCCCGCTGCGCTGACACACGTCGACCGTACCGGCAATGGCGCCGAAGGCGGCACCGCCT
CGCCTTAAACTTGGCTCTCT

Downstream 100 bases:

>100_bases
GCGGGAAAGCCGGGACGCAGCCAGAGCCCAGATCCCATGGCATCATTGCTTGCATCGAGTGATGCATGTATTGATGGGAG
TTGGTGAATGAGGACGACGG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVVSLPDPRLDKLAELFGSQRVVPAPVTFVDIAGLVKGA
SEGAGLGNKFLAHIRECDAICQVVRVFVDDDVTHVTGRVDPQSDIEVVETELILADLQTLERATGRLEKEARTNKARKPV
YDAALRAQQVLDAGKTLFAAGVDAAALRELNLLTTKPFLYVFNADEAVLTDPARVGELRALVAPADAVFLDAAIESELTE
LDDESAAELLESIGQSERGLDALARAGFHTLKLQTFLTAGPKEARAWTIHQGDTAPKAAGVIHSDFEKGFIKAEIVSYDD
LVAAGSMAAAKAAGKVRIEGKDYVMADGDVVEFRFNV

Sequences:

>Translated_357_residues
MSLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVVSLPDPRLDKLAELFGSQRVVPAPVTFVDIAGLVKGA
SEGAGLGNKFLAHIRECDAICQVVRVFVDDDVTHVTGRVDPQSDIEVVETELILADLQTLERATGRLEKEARTNKARKPV
YDAALRAQQVLDAGKTLFAAGVDAAALRELNLLTTKPFLYVFNADEAVLTDPARVGELRALVAPADAVFLDAAIESELTE
LDDESAAELLESIGQSERGLDALARAGFHTLKLQTFLTAGPKEARAWTIHQGDTAPKAAGVIHSDFEKGFIKAEIVSYDD
LVAAGSMAAAKAAGKVRIEGKDYVMADGDVVEFRFNV
>Mature_356_residues
SLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVVSLPDPRLDKLAELFGSQRVVPAPVTFVDIAGLVKGAS
EGAGLGNKFLAHIRECDAICQVVRVFVDDDVTHVTGRVDPQSDIEVVETELILADLQTLERATGRLEKEARTNKARKPVY
DAALRAQQVLDAGKTLFAAGVDAAALRELNLLTTKPFLYVFNADEAVLTDPARVGELRALVAPADAVFLDAAIESELTEL
DDESAAELLESIGQSERGLDALARAGFHTLKLQTFLTAGPKEARAWTIHQGDTAPKAAGVIHSDFEKGFIKAEIVSYDDL
VAAGSMAAAKAAGKVRIEGKDYVMADGDVVEFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=43.75, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI58761502, Length=214, Percent_Identity=37.8504672897196, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=50.958904109589, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789574, Length=130, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=41.8032786885246, Blast_Score=279, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=40, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321773, Length=383, Percent_Identity=36.5535248041775, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321649, Length=177, Percent_Identity=34.4632768361582, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640873, Length=367, Percent_Identity=44.4141689373297, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24640877, Length=367, Percent_Identity=44.4141689373297, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24640875, Length=367, Percent_Identity=44.4141689373297, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=42.4615384615385, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24585318, Length=184, Percent_Identity=29.3478260869565, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 38098; Mature: 37966

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVVSLPDPRLDKLAELFGS
CCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEECCCCHHHHHHHHHCC
QRVVPAPVTFVDIAGLVKGASEGAGLGNKFLAHIRECDAICQVVRVFVDDDVTHVTGRVD
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PQSDIEVVETELILADLQTLERATGRLEKEARTNKARKPVYDAALRAQQVLDAGKTLFAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECC
GVDAAALRELNLLTTKPFLYVFNADEAVLTDPARVGELRALVAPADAVFLDAAIESELTE
CCCHHHHHHHHHEECCCEEEEEECCCEEECCCHHHHHHHHEECCCHHHHHHHHHHHHHHH
LDDESAAELLESIGQSERGLDALARAGFHTLKLQTFLTAGPKEARAWTIHQGDTAPKAAG
CCCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEECCCCCCHHHC
VIHSDFEKGFIKAEIVSYDDLVAAGSMAAAKAAGKVRIEGKDYVMADGDVVEFRFNV
CHHHHHHCCCEEEEEECHHHHHHHCCHHHHHCCCEEEECCCCEEEECCCEEEEEECC
>Mature Secondary Structure 
SLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVVSLPDPRLDKLAELFGS
CEEEEEEECCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEECCCCHHHHHHHHHCC
QRVVPAPVTFVDIAGLVKGASEGAGLGNKFLAHIRECDAICQVVRVFVDDDVTHVTGRVD
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PQSDIEVVETELILADLQTLERATGRLEKEARTNKARKPVYDAALRAQQVLDAGKTLFAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECC
GVDAAALRELNLLTTKPFLYVFNADEAVLTDPARVGELRALVAPADAVFLDAAIESELTE
CCCHHHHHHHHHEECCCEEEEEECCCEEECCCHHHHHHHHEECCCHHHHHHHHHHHHHHH
LDDESAAELLESIGQSERGLDALARAGFHTLKLQTFLTAGPKEARAWTIHQGDTAPKAAG
CCCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEECCCCCCHHHC
VIHSDFEKGFIKAEIVSYDDLVAAGSMAAAKAAGKVRIEGKDYVMADGDVVEFRFNV
CHHHHHHCCCEEEEEECHHHHHHHCCHHHHHCCCEEEECCCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]