Click here to switch to the map view.

The map label for this gene is citA

Identifier: 148660667

GI number: 148660667

Start: 990047

End: 991168

Strand: Reverse

Name: citA

Synonym: MRA_0897

Alternate gene names: 148660667

Gene position: 991168-990047 (Counterclockwise)

Preceding gene: 148660668

Following gene: 148660665

Centisome position: 22.42

GC content: 68.63

Gene sequence:

>1122_bases
ATGACAGTGGTCCCGGAGAATTTCGTCCCCGGCCTCGACGGCGTGGTGGCCTTTACGACCGAGATCGCCGAGCCGGACAA
AGACGGCGGGGCCCTGCGCTACCGTGGCGTCGACATCGAAGACCTGGTAAGTCAGCGGGTCACCTTCGGCGATGTGTGGG
CGCTGCTGGTGGACGGCAACTTCGGCAGCGGGCTGCCGCCGGCTGAACCGTTCCCGCTGCCGATTCACTCCGGCGATGTG
CGCGTCGACGTCCAGGCCGGCCTGGCGATGCTGGCGCCCATCTGGGGATATGCGCCGCTGCTCGACATCGACGACGCCAC
CGCCCGCCAACAGCTGGCCCGGGCATCGGTGATGGCGCTGTCCTATGTCGCGCAATCCGCACGGGGCATCTACCAGCCGG
CGGTCCCGCAGCGAATCATCGATGAATGCTCGACGGTCACAGCACGTTTCATGACGCGCTGGCAGGGCGAGCCAGACCCC
AGACACATCGAGGCCATTGACGCCTACTGGGTGTCGGCCGCCGAGCACGGCATGAACGCTTCGACATTCACCGCGCGTGT
GATCGCCTCAACTGGGGCGGATGTGGCAGCAGCGCTGTCGGGCGCAATCGGGGCGATGAGCGGACCGCTGCATGGTGGCG
CGCCGGCGCGCGTCCTGCCGATGCTCGACGAGGTCGAACGCGCCGGCGATGCCCGCAGCGTGGTCAAGGGAATCCTGGAC
CGCGGCGAGAAACTGATGGGGTTCGGGCACCGGGTCTACCGCGCCGAGGACCCGCGGGCGCGGGTGCTGCGGGCGGCCGC
CGAGCGGCTGGGCGCCCCCCGCTACGAGGTCGCGGTCGCGGTGGAGCAGGCTGCGCTGTCAGAACTGCGAGAGCGCCGTC
CGGATCGGGCGATCGAGACCAATGTCGAATTCTGGGCCGCAGTGGTCCTGGACTTTGCCCGGGTACCGGCCAACATGATG
CCAGCAATGTTCACCTGTGGGCGCACTGCCGGCTGGTGTGCCCACATTCTCGAGCAGAAGCGACTCGGCAAGCTGGTCCG
CCCGTCGGCCATTTACGTGGGACCGGGCCCGCGCAGCCCGGAATCGGTCGACGGCTGGGAGCGGGTACTCACCACCGCCT
GA

Upstream 100 bases:

>100_bases
GCTCACCCTCGTGACGATTCTCGCGGCACGCTCATCGCCGGCACCGTGATTGGCACCAGCCGGTAGCGTCGTTGCCGGGG
CCAGGTGTGAGAATTCTCGT

Downstream 100 bases:

>100_bases
GGCCGACGGCAAACCAGTTGGCGATCAACCGCTGTCACCGTACGGCCACGTTGTCCGCGACGTAGTGGAAGCCGACCACC
GCCGGGCTGTGATCCGACAG

Product: citrate synthase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGNFGSGLPPAEPFPLPIHSGDV
RVDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMALSYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDP
RHIEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILD
RGEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIETNVEFWAAVVLDFARVPANMM
PAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSPESVDGWERVLTTA

Sequences:

>Translated_373_residues
MTVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGNFGSGLPPAEPFPLPIHSGDV
RVDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMALSYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDP
RHIEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILD
RGEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIETNVEFWAAVVLDFARVPANMM
PAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSPESVDGWERVLTTA
>Mature_372_residues
TVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGNFGSGLPPAEPFPLPIHSGDVR
VDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMALSYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDPR
HIEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILDR
GEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIETNVEFWAAVVLDFARVPANMMP
AMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSPESVDGWERVLTTA

Specific function: Catalyzes The Synthesis Of 2-Methylcitrate From Propionyl-CoA And Oxaloacetate. Also Catalyzes The Condensation Of Oxaloacetate With Acetyl-CoA But With A Lower Specificity. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=31.5508021390374, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786939, Length=392, Percent_Identity=27.8061224489796, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CISY2_MYCBO (P63778)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854570.1
- ProteinModelPortal:   P63778
- SMR:   P63778
- EnsemblBacteria:   EBMYCT00000014236
- GeneID:   1092810
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0913c
- GeneTree:   EBGT00050000016202
- HOGENOM:   HBG649489
- OMA:   VWGLLVD
- ProtClustDB:   PRK12350
- BioCyc:   MBOV233413:MB0913C-MONOMER
- BRENDA:   2.3.3.1
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 40147; Mature: 40016

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 250-250 ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGN
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCEEEEEEECC
FGSGLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMAL
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
SYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDPRHIEAIDAYWVSAAEHGMNA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
STFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILD
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
RGEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIET
CCHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
NVEFWAAVVLDFARVPANMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSP
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
ESVDGWERVLTTA
CCCHHHHHHHHCC
>Mature Secondary Structure 
TVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGN
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCEEEEEEECC
FGSGLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMAL
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
SYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDPRHIEAIDAYWVSAAEHGMNA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
STFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILD
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
RGEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIET
CCHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
NVEFWAAVVLDFARVPANMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSP
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
ESVDGWERVLTTA
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972