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The map label for this gene is merA [H]

Identifier: 148660572

GI number: 148660572

Start: 888443

End: 889942

Strand: Reverse

Name: merA [H]

Synonym: MRA_0804

Alternate gene names: 148660572

Gene position: 889942-888443 (Counterclockwise)

Preceding gene: 148660576

Following gene: 148660570

Centisome position: 20.13

GC content: 67.4

Gene sequence:

>1500_bases
ATGACCGCGGCCCAACAGGACCAGGCGCCAATGGCAACACCCGGCTGCCGTGAGGGTGAAACGTATGACGTCGTCGTGCT
CGGCGCGGGACCCGTTGGACAGAACGTCGCCGATCGTGCCCGCGCGGGGGGCCTGCGTGTCGCGGTGGTGGAGCGCGAAC
TCGTCGGGGGTGAATGCTCCTATTGGGCCTGTGTGCCCAGCAAAGCCTTGCTGCGTCCGGTCATCGCGATCTCTGACGCC
CGACGGGTCGACGGCGCGCGCGAAGCAGTCGACGGCTCGATCAACACAGCCGGCGTCTTTGGCCGCCGCAACCGCTATGT
GGCCCACTGGGACGACACCGGCCAGGCCGACTGGGTGAGTGGAATCGGCGCGACGCTGATACGCGGTGACGGGCGATTGG
ACGGTCCGCGCCGCGTCGTCGTCACCAAGTCGAGCGGCGAAAGCGTGGCGCTGACCGCCCGGCATGCCGTTGTCATCTGC
ACCGGAAGCCGGCCAGCACTCCCCGACCTTCCTGGCATCACCGAAGCCCGGCCATGGACCAATCGCCAAGCCACCGACAA
CAGTACGGTCCCCGACCGGCTTGCGATCGTCGGCGCCGGCGGCGTCGGTGTGGAGATGGCGACCGCCTGGCAGGGACTGG
GCGCCTCGGTGACCCTGCTGGCTCGGGGATCTGGCCTGCTGCCCCGAATGGAACCGTTTGTGGGGGAACTCATCGGTCGC
GGACTGGCCGACGCCGGCGTTGACGTGCGCGTGGGAGTATCGGTACGCGCGCTGGGCCGCCCCAACCCAACTGGCCCAGT
GGTCCTCGAGCTGGACGACGGTACCGAGCTGCGGGTCGACGAGGTACTCTTCGCCACCGGCCGAGCACCGCGAACCGACG
ACATCGGCTTGGAGACAATAGGACTGACGCCGGGCAGCTGGCTGGACGTCGATGACACCTGCCGAGTGCGGGCTGTTGAC
GACGGCTGGCTCTATGCCGCCGGCGACGTCAACCATCGCGCGTTGCTGACCCACCAAGGCAAATACCAGGCGCGGATCGC
CGGCACCGCGATCGGCGCCCGTGCCGCCGGACGACCGCTAGACACCACGTCGTGGGGCATGCACGCGACCACCGCCGACC
ATCACGCGGTGCCGCAGGCATTCTTTACCGACCCCGAAGCCGCAGCGGTCGGCCTGACAGCTGATCAGGCCGCACAGGCT
GGTCACCGGATCAAAGCGATCGATGTCGAAATCGGCGATGTCGTTATGGGAGCCAAGCTCTTTGCCGACGGATACACCGG
CAGGGCGCGCATGGTGGTCGACGTCGATCGGGGCCATCTGCTGGGCGTGACCATGGTTGGCCCGGGCGCCGCCGAGCTGT
TGCATTCGGCCACCGTCGCCGTCGCCGGCCAGGTGCCAATCGATCGGTTGTGGCACGCCGTTCCGTGCTTCCCGACCATC
AGCGAACTGTGGCTGAGACTTCTTGAATCCTACCGAGATTCGTTTTACCTGCTGGTATAG

Upstream 100 bases:

>100_bases
TCGAGAGCTTTTGCGTCTGGTGGGCGATAGGCCGGCACGGCTCACCGGCGCTAGGCGCGCGTAGCGTCGCTGGCAGAGTC
CGACGAAAGGATCTTTGATT

Downstream 100 bases:

>100_bases
CCAACCCGCCGCCGCGCCGCTGAACCCACGGGGGGACTGCGGTGGTCTGCGGCGGTTCCCGAGCGCTCGGCCGGTGCCGG
GCGTGGATCAAGCTGCCTGG

Product: putative oxidoreductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDA
RRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVIC
TGSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
GLADAGVDVRVGVSVRALGRPNPTGPVVLELDDGTELRVDEVLFATGRAPRTDDIGLETIGLTPGSWLDVDDTCRVRAVD
DGWLYAAGDVNHRALLTHQGKYQARIAGTAIGARAAGRPLDTTSWGMHATTADHHAVPQAFFTDPEAAAVGLTADQAAQA
GHRIKAIDVEIGDVVMGAKLFADGYTGRARMVVDVDRGHLLGVTMVGPGAAELLHSATVAVAGQVPIDRLWHAVPCFPTI
SELWLRLLESYRDSFYLLV

Sequences:

>Translated_499_residues
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDA
RRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVIC
TGSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
GLADAGVDVRVGVSVRALGRPNPTGPVVLELDDGTELRVDEVLFATGRAPRTDDIGLETIGLTPGSWLDVDDTCRVRAVD
DGWLYAAGDVNHRALLTHQGKYQARIAGTAIGARAAGRPLDTTSWGMHATTADHHAVPQAFFTDPEAAAVGLTADQAAQA
GHRIKAIDVEIGDVVMGAKLFADGYTGRARMVVDVDRGHLLGVTMVGPGAAELLHSATVAVAGQVPIDRLWHAVPCFPTI
SELWLRLLESYRDSFYLLV
>Mature_498_residues
TAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDAR
RVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICT
GSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGRG
LADAGVDVRVGVSVRALGRPNPTGPVVLELDDGTELRVDEVLFATGRAPRTDDIGLETIGLTPGSWLDVDDTCRVRAVDD
GWLYAAGDVNHRALLTHQGKYQARIAGTAIGARAAGRPLDTTSWGMHATTADHHAVPQAFFTDPEAAAVGLTADQAAQAG
HRIKAIDVEIGDVVMGAKLFADGYTGRARMVVDVDRGHLLGVTMVGPGAAELLHSATVAVAGQVPIDRLWHAVPCFPTIS
ELWLRLLESYRDSFYLLV

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=484, Percent_Identity=27.8925619834711, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI50301238, Length=494, Percent_Identity=26.1133603238866, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=22.6012793176972, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=22.6012793176972, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=22.6012793176972, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=22.6012793176972, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=22.6012793176972, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=487, Percent_Identity=25.0513347022587, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=25.5913978494624, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI87082354, Length=490, Percent_Identity=25.1020408163265, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081717, Length=492, Percent_Identity=25, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32565766, Length=485, Percent_Identity=27.2164948453608, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321091, Length=496, Percent_Identity=25.4032258064516, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325166, Length=482, Percent_Identity=23.8589211618257, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358499, Length=486, Percent_Identity=27.5720164609054, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640549, Length=501, Percent_Identity=24.5508982035928, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640551, Length=506, Percent_Identity=24.5059288537549, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640553, Length=501, Percent_Identity=24.5508982035928, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 52477; Mature: 52346

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECS
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCEEEEEEEEECCCCCCC
YWACVPSKALLRPVIAISDARRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVS
EEEECCCHHHHHHHHEECCCHHCCCHHHHHCCCCCCEEEECCCCCEEEECCCCCCCHHHH
GIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICTGSRPALPDLPGITEARPWT
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
NRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCC
GLADAGVDVRVGVSVRALGRPNPTGPVVLELDDGTELRVDEVLFATGRAPRTDDIGLETI
CCCCCCCEEEECEEEEECCCCCCCCCEEEEECCCCCEEEEHEEEECCCCCCCCCCCEEEE
GLTPGSWLDVDDTCRVRAVDDGWLYAAGDVNHRALLTHQGKYQARIAGTAIGARAAGRPL
ECCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEEEEEEEECHHCCCCCC
DTTSWGMHATTADHHAVPQAFFTDPEAAAVGLTADQAAQAGHRIKAIDVEIGDVVMGAKL
CCCCCCCEECCCCCCCCCHHHCCCCCCEEEECCHHHHHHCCCEEEEEEEEECCEEECEEE
FADGYTGRARMVVDVDRGHLLGVTMVGPGAAELLHSATVAVAGQVPIDRLWHAVPCFPTI
EECCCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHCEEEEECCCCHHHHHHHCCCCCCH
SELWLRLLESYRDSFYLLV
HHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
TAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECS
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCEEEEEEEEECCCCCCC
YWACVPSKALLRPVIAISDARRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVS
EEEECCCHHHHHHHHEECCCHHCCCHHHHHCCCCCCEEEECCCCCEEEECCCCCCCHHHH
GIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICTGSRPALPDLPGITEARPWT
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
NRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCC
GLADAGVDVRVGVSVRALGRPNPTGPVVLELDDGTELRVDEVLFATGRAPRTDDIGLETI
CCCCCCCEEEECEEEEECCCCCCCCCEEEEECCCCCEEEEHEEEECCCCCCCCCCCEEEE
GLTPGSWLDVDDTCRVRAVDDGWLYAAGDVNHRALLTHQGKYQARIAGTAIGARAAGRPL
ECCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEEEEEEEECHHCCCCCC
DTTSWGMHATTADHHAVPQAFFTDPEAAAVGLTADQAAQAGHRIKAIDVEIGDVVMGAKL
CCCCCCCEECCCCCCCCCHHHCCCCCCEEEECCHHHHHHCCCEEEEEEEEECCEEECEEE
FADGYTGRARMVVDVDRGHLLGVTMVGPGAAELLHSATVAVAGQVPIDRLWHAVPCFPTI
EECCCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHCEEEEECCCCHHHHHHHCCCCCCH
SELWLRLLESYRDSFYLLV
HHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]