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The map label for this gene is emrB

Identifier: 148660560

GI number: 148660560

Start: 878124

End: 879746

Strand: Reverse

Name: emrB

Synonym: MRA_0792

Alternate gene names: 148660560

Gene position: 879746-878124 (Counterclockwise)

Preceding gene: 148660563

Following gene: 148660556

Centisome position: 19.9

GC content: 63.59

Gene sequence:

>1623_bases
ATGCTCGGCAACGCCATGGTTGAAGCGTGTCCCGCGGAAGGCGATGCCCCGGTGCCCATCACCCCCGCGGGGCGGCCCCG
CTCTGGCCAACGCAGCTATCCCGATAGGCTCGATGTGGGGTTGCTTCGGACCGCCGGAGTGTGTGTCCTGGCCTCGGTGA
TGGCACATGTGGACGTCACCGTGGTCAGCGTCGCGCAACGCACCTTCGTTGCCGATTTCGGGTCCACCCAGGCCGTCGTG
GCCTGGACCATGACCGGCTACATGCTCGCGTTGGCCACTGTGATCCCGACAGCCGGTTGGGCGGCCGACCGGTTCGGCAC
CAGGCGGCTTTTCATGGGTTCGGTATTGGCGTTCACCCTCGGCTCACTGCTGTGCGCAGTAGCACCAAACATCTTGCTGC
TCATCATATTTCGTGTTGTCCAGGGTTTCGGTGGGGGCATGCTGACGCCGGTGTCCTTTGCCATCTTGGCCCGTGAGGCG
GGCCCGAAGCGGCTGGGGCGCGTGATGGCGGTGGTGGGCATTCCGATGCTGCTCGGCCCGGTCGGTGGGCCGATCCTGGG
TGGTTGGCTCATCGGGGCCTACGGCTGGCGTTGGATTTTCTTGGTCAACCTGCCGGTCGGGCTGTCCGCGCTCGTCTTGG
CGGCGATCGTGTTCCCAAGAGATCGCCCGGCAGCGTCGGAAAACTTCGACTACATGGGCCTCTTGTTGCTGTCGCCGGGC
CTGGCGACCTTCCTGTTCGGGGTGTCATCTAGCCCCGCCCGTGGAACGATGGCCGATCGGCACGTGTTGATACCGGCGAT
CACCGGCCTGGCGTTGATCGCGGCATTCGTCGCACATTCGTGGTACCGCACAGAACATCCGCTCATAGACATGCGCTTGT
TCCAGAACCGAGCGGTCGCGCAGGCCAACATGACGATGACGGTGCTCTCCCTCGGGCTGTTTGGCTCCTTCTTGCTGCTC
CCGAGCTACCTCCAGCAAGTGTTGCACCAATCACCGATGCAATCGGGGGTGCATATCATCCCACAGGGCCTCGGTGCCAT
GCTGGCGATGCCGATCGCCGGAGCGATGATGGACCGACGGGGACCGGCCAAGATCGTGCTGGTTGGGATCATGCTGATCG
CTGCGGGGTTGGGCACCTTCGCCTTTGGTGTCGCGCGGCAAGCGGACTACTTACCCATTCTGCCGACCGGGCTGGCAATC
ATGGGCATGGGCATGGGCTGCTCCATGATGCCACTGTCCGGGGCGGCAGTGCAGACCCTGGCCCCACATCAGATCGCTCG
CGGTTCGACGCTGATCAGCGTCAACCAGCAGGTGGGCGGTTCGATAGGGACCGCACTGATGTCGGTGCTGCTCACCTACC
AGTTCAATCACAGCGAAATCATCGCTACTGCAAAGAAAGTCGCACTGACCCCAGAGAGTGGCGCCGGGCGGGGGGCGGCG
GTTGACCCTTCCTCGCTACCGCGCCAAACCAACTTCGCGGCCCAACTGCTGCATGACCTTTCGCACGCCTACGCGGTGGT
ATTCGTGATAGCGACCGCGCTAGTGGTCTCGACGCTGATCCCCGCGGCATTCCTGCCGAAACAGCAGGCTAGTCATCGAA
GAGCACCGTTGCTATCCGCATGA

Upstream 100 bases:

>100_bases
GCAGAACGAGCGCACGTCGGCTCGGCGCCAGGGCCATGACCCGACGCCGGAACCAAGCACCGCCCGACATCCTCGCGCGG
TACCCGTGACCGGTATCGAT

Downstream 100 bases:

>100_bases
CGTCTGCTTCACGTCAGCGTTGACCGCATCGTCGAGCCACAGCGTCGAAAAGCCGTGCACCAGCGACCAGGTCGCCAGCT
CGGCACCCGCCGGATTCGCC

Product: multidrug resistance integral membrane efflux protein EmrB

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVTVVSVAQRTFVADFGSTQAVV
AWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREA
GPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPG
LATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVAQANMTMTVLSLGLFGSFLLL
PSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAI
MGMGMGCSMMPLSGAAVQTLAPHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAA
VDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA

Sequences:

>Translated_540_residues
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVTVVSVAQRTFVADFGSTQAVV
AWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREA
GPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPG
LATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVAQANMTMTVLSLGLFGSFLLL
PSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAI
MGMGMGCSMMPLSGAAVQTLAPHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAA
VDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA
>Mature_540_residues
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVTVVSVAQRTFVADFGSTQAVV
AWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREA
GPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPG
LATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVAQANMTMTVLSLGLFGSFLLL
PSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAI
MGMGMGCSMMPLSGAAVQTLAPHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAA
VDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=31.219512195122, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789042, Length=424, Percent_Identity=27.122641509434, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788710, Length=450, Percent_Identity=27.1111111111111, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=27.3170731707317, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788392, Length=412, Percent_Identity=27.6699029126214, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1790146, Length=187, Percent_Identity=26.2032085561497, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=497, Percent_Identity=23.7424547283702, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=372, Percent_Identity=22.8494623655914, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=32.6666666666667, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=327, Percent_Identity=24.4648318042813, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMRB_MYCTU (P71836)

Other databases:

- EMBL:   BX842574
- EMBL:   AE000516
- PIR:   B70709
- RefSeq:   NP_215297.1
- RefSeq:   NP_335235.1
- ProteinModelPortal:   P71836
- EnsemblBacteria:   EBMYCT00000002313
- EnsemblBacteria:   EBMYCT00000070183
- GeneID:   885836
- GeneID:   926110
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0807
- KEGG:   mtu:Rv0783c
- TIGR:   MT0807
- TubercuList:   Rv0783c
- GeneTree:   EBGT00050000014781
- HOGENOM:   HBG679341
- OMA:   HAWHRAD
- ProtClustDB:   CLSK871854
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 56588; Mature: 56588

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c87f66c)-; HASH(0x1cd522a8)-; HASH(0x1bd3a40c)-; HASH(0x1d273054)-; HASH(0x1c4c5798)-; HASH(0x1c7c98c4)-; HASH(0x1c7cd738)-; HASH(0x1ae92d60)-; HASH(0x1d2bc7d4)-; HASH(0x1ca6dd50)-; HASH(0x1cdc6548)-; HASH(0x1d2ecf8c)-; HASH(0x1c528b74)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVT
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHH
VVSVAQRTFVADFGSTQAVVAWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
GSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREAGPKRLGRVMAVVGIPMLLGP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
VGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPG
CCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCC
LATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVA
HHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCHHHH
QANMTMTVLSLGLFGSFLLLPSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEHHHHHHHHHHHHHHHHHCCC
GPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAIMGMGMGCSMMPLSGAAVQTL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHC
APHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAA
CCHHHHCCCEEEEECHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCC
VDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCH
>Mature Secondary Structure
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVT
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHH
VVSVAQRTFVADFGSTQAVVAWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
GSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREAGPKRLGRVMAVVGIPMLLGP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
VGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPG
CCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCC
LATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVA
HHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCHHHH
QANMTMTVLSLGLFGSFLLLPSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEHHHHHHHHHHHHHHHHHCCC
GPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAIMGMGMGCSMMPLSGAAVQTL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHC
APHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAA
CCHHHHCCCEEEEECHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCC
VDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036