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The map label for this gene is nolF [H]

Identifier: 148654150

GI number: 148654150

Start: 2928847

End: 2930175

Strand: Direct

Name: nolF [H]

Synonym: PsycPRwf_2353

Alternate gene names: 148654150

Gene position: 2928847-2930175 (Clockwise)

Preceding gene: 148654149

Following gene: 148654151

Centisome position: 98.32

GC content: 50.04

Gene sequence:

>1329_bases
ATGACTTCTTATTCTGATCCCAATAAAAAGACCCCTGATGAGGCGTCTACTGAGCTTAAGGTGGATGCAGCTGCTGCTAC
CCAAGGCTCTGATGATTTCAAGCCTTATACTGCCGCAGAAACCAAAGCCAAACCAAACTGGCTATGGGTATTACCCCTTA
TTTTATTGACATTGGCGGTTGGTCTATTTGCCGGTCGTATGTGGGGCCAAAAGCAACTTGAAGACAACAGCGCAGAAACG
CAGAGATCTGTCGTGCCTGAAGGCGGTACAGCTACTGGTATCACTGAAATAGAAAAGCCGGTCAATCAAAAGCCGGTACT
GACGGTTGAAACCGTTACCCCAACTCAGTCAAGTGTGGCCAATGACATCTCAGCAGACGGTACCATCAGTCCTGAGACTA
CCTCTAGTGTGAGCGGAAAAGTAAGTGGGGTAGCCATTGATCAGGTTCTGGTACAAGAGGGAGCCCGAGTCAAAAAAGGA
CAGGTATTGGCCGTCTTTGATACCGATGCCATGCGCCAACAAATTATTCAGTCTCAAGCTGAGCTGGCAGAAGCTCAGTC
TAGCTATGACTTGGCCAAGCGAGATGCCGAGCGGGTACTACCTTTATTAGAGATTAATGCCATTAGCCAGCAAGAAGCTG
ATCGTTATGTGGCCACTGCCAATCAAGCTGCCGCTTCTGTGGCTGCCTCTAAGGCGCGCTTGAACAATCAAAACCTAAAT
ATGCAAAATGCCAAAGTAGTGGCACCCGTCAGCGGGGTCATTAGCGAAAAAAATGCCAATGTTGGCAGCGTTCCTGGTCC
TGAGCCTTTGTTCACCATTATCGAGGAGGGCAAGCTTGAGTGGCAGGCCCAAGTGGATCCTAATAAAGTGGGAGAAATCA
ATATTGGTACGCCGGTGGAAGTGAGCCTACCCAATAATAAGAGCGTGGCCGGTAAGGTCACTCGTATTTCACCCACTGCT
GAAGATGGCAGCCGCCAAATTACTATTTATGCCGCTTTAGCGCCTAATCCCAATGCGCGCGCAGGGATGTATCAACGCGG
CACCTTTAAGCTTGGTAGCGATGCCAAAGTGACCATTCCTGTCAGTGCTGTGGTCAGTGAAGATGGCTATGATTACGTGA
TGCAGGTCAAAGCGGTGAAGGATGAAAATCGCGACACCATCTACCGCGTCAACCGAGTCAAAGTGAAGCTGGGTGAGCGT
CAAGGCGACCGTGTGGTTCTGCTTGAGCCGCTACAAGGTGACGGCAATATCGTACGTCAAGGTGGCAGCTTCTTAAGTGA
CGGTGATGTGGTAACTTTGGCCACAGCCACACCCGAGCCAGCTAAGTAA

Upstream 100 bases:

>100_bases
AAAAATTTACTACGATATCGCTCTAAACATAAGAGATATATTTAACGTATAATTTAAATTATTTTTTATTTATCCAAACC
CCAGTGTTTGCGAGACTAAT

Downstream 100 bases:

>100_bases
GTAGCTTGAGGGTATCATCTATTCGCTGATACGACGGGACATCGATGTTGATGTCCTGTCCTTGCCATCGACAGTGACTA
TTTGTGCTGATCCTCAAGTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSYSDPNKKTPDEASTELKVDAAAATQGSDDFKPYTAAETKAKPNWLWVLPLILLTLAVGLFAGRMWGQKQLEDNSAET
QRSVVPEGGTATGITEIEKPVNQKPVLTVETVTPTQSSVANDISADGTISPETTSSVSGKVSGVAIDQVLVQEGARVKKG
QVLAVFDTDAMRQQIIQSQAELAEAQSSYDLAKRDAERVLPLLEINAISQQEADRYVATANQAAASVAASKARLNNQNLN
MQNAKVVAPVSGVISEKNANVGSVPGPEPLFTIIEEGKLEWQAQVDPNKVGEINIGTPVEVSLPNNKSVAGKVTRISPTA
EDGSRQITIYAALAPNPNARAGMYQRGTFKLGSDAKVTIPVSAVVSEDGYDYVMQVKAVKDENRDTIYRVNRVKVKLGER
QGDRVVLLEPLQGDGNIVRQGGSFLSDGDVVTLATATPEPAK

Sequences:

>Translated_442_residues
MTSYSDPNKKTPDEASTELKVDAAAATQGSDDFKPYTAAETKAKPNWLWVLPLILLTLAVGLFAGRMWGQKQLEDNSAET
QRSVVPEGGTATGITEIEKPVNQKPVLTVETVTPTQSSVANDISADGTISPETTSSVSGKVSGVAIDQVLVQEGARVKKG
QVLAVFDTDAMRQQIIQSQAELAEAQSSYDLAKRDAERVLPLLEINAISQQEADRYVATANQAAASVAASKARLNNQNLN
MQNAKVVAPVSGVISEKNANVGSVPGPEPLFTIIEEGKLEWQAQVDPNKVGEINIGTPVEVSLPNNKSVAGKVTRISPTA
EDGSRQITIYAALAPNPNARAGMYQRGTFKLGSDAKVTIPVSAVVSEDGYDYVMQVKAVKDENRDTIYRVNRVKVKLGER
QGDRVVLLEPLQGDGNIVRQGGSFLSDGDVVTLATATPEPAK
>Mature_441_residues
TSYSDPNKKTPDEASTELKVDAAAATQGSDDFKPYTAAETKAKPNWLWVLPLILLTLAVGLFAGRMWGQKQLEDNSAETQ
RSVVPEGGTATGITEIEKPVNQKPVLTVETVTPTQSSVANDISADGTISPETTSSVSGKVSGVAIDQVLVQEGARVKKGQ
VLAVFDTDAMRQQIIQSQAELAEAQSSYDLAKRDAERVLPLLEINAISQQEADRYVATANQAAASVAASKARLNNQNLNM
QNAKVVAPVSGVISEKNANVGSVPGPEPLFTIIEEGKLEWQAQVDPNKVGEINIGTPVEVSLPNNKSVAGKVTRISPTAE
DGSRQITIYAALAPNPNARAGMYQRGTFKLGSDAKVTIPVSAVVSEDGYDYVMQVKAVKDENRDTIYRVNRVKVKLGERQ
GDRVVLLEPLQGDGNIVRQGGSFLSDGDVVTLATATPEPAK

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=376, Percent_Identity=24.2021276595745, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082036, Length=159, Percent_Identity=28.9308176100629, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081951, Length=201, Percent_Identity=27.8606965174129, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47080; Mature: 46949

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYSDPNKKTPDEASTELKVDAAAATQGSDDFKPYTAAETKAKPNWLWVLPLILLTLAV
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
GLFAGRMWGQKQLEDNSAETQRSVVPEGGTATGITEIEKPVNQKPVLTVETVTPTQSSVA
HHHHHHHCCCHHCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCHHHHH
NDISADGTISPETTSSVSGKVSGVAIDQVLVQEGARVKKGQVLAVFDTDAMRQQIIQSQA
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELAEAQSSYDLAKRDAERVLPLLEINAISQQEADRYVATANQAAASVAASKARLNNQNLN
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQNAKVVAPVSGVISEKNANVGSVPGPEPLFTIIEEGKLEWQAQVDPNKVGEINIGTPVE
CCCCEEEECCHHHHCCCCCCCCCCCCCCHHHHEEECCCEEEEEECCCCCCCEEECCCCEE
VSLPNNKSVAGKVTRISPTAEDGSRQITIYAALAPNPNARAGMYQRGTFKLGSDAKVTIP
EECCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCEECCCEEECCCCEEEEE
VSAVVSEDGYDYVMQVKAVKDENRDTIYRVNRVKVKLGERQGDRVVLLEPLQGDGNIVRQ
EEEEECCCCCEEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEC
GGSFLSDGDVVTLATATPEPAK
CCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TSYSDPNKKTPDEASTELKVDAAAATQGSDDFKPYTAAETKAKPNWLWVLPLILLTLAV
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
GLFAGRMWGQKQLEDNSAETQRSVVPEGGTATGITEIEKPVNQKPVLTVETVTPTQSSVA
HHHHHHHCCCHHCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCHHHHH
NDISADGTISPETTSSVSGKVSGVAIDQVLVQEGARVKKGQVLAVFDTDAMRQQIIQSQA
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELAEAQSSYDLAKRDAERVLPLLEINAISQQEADRYVATANQAAASVAASKARLNNQNLN
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQNAKVVAPVSGVISEKNANVGSVPGPEPLFTIIEEGKLEWQAQVDPNKVGEINIGTPVE
CCCCEEEECCHHHHCCCCCCCCCCCCCCHHHHEEECCCEEEEEECCCCCCCEEECCCCEE
VSLPNNKSVAGKVTRISPTAEDGSRQITIYAALAPNPNARAGMYQRGTFKLGSDAKVTIP
EECCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCEECCCEEECCCCEEEEE
VSAVVSEDGYDYVMQVKAVKDENRDTIYRVNRVKVKLGERQGDRVVLLEPLQGDGNIVRQ
EEEEECCCCCEEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEC
GGSFLSDGDVVTLATATPEPAK
CCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]