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The map label for this gene is dusC [H]

Identifier: 148654121

GI number: 148654121

Start: 2891708

End: 2892679

Strand: Direct

Name: dusC [H]

Synonym: PsycPRwf_2324

Alternate gene names: 148654121

Gene position: 2891708-2892679 (Clockwise)

Preceding gene: 148654120

Following gene: 148654125

Centisome position: 97.07

GC content: 51.65

Gene sequence:

>972_bases
ATGTGTTTTTCTGACCAGTTTTTTTCATCGCCTGTCCGTCTTTTGGCGCCCATGGAGGGGCTTACAGACCCACTGATGCG
CCAAATACTCACCCAAATTGCCAGCGACTTGGGTCGCCCTTATGATTGGTCGGTCAGTGAGTTTATCCGTATTACTCATC
ATGTCTTGCCTGAGCATGTCTTTTACCGCTTCGTCCCTGAGCTGTTGCAGTCAGGCACAACCGATAGCGGCACCCCTATT
CATGTGCAGCTGCTTGGTAGTGAGCCTAAGCTTATGGGCGCCAATGCCTTGGCAGCCGCTGAGCTTGGCGCACCAGCAAT
TGACCTAAATTTTGGCTGTCCAGCAAAAACGGTGAACAATCACCGCGGCGGCAGTGTGCTGCTCGATGAGCCACGCCTCA
TGCGTGATATCATCTCTGCGGTGCGAGAGGCTGTGCCGGCTCACATTCCGGTCTCGGCTAAAATTCGCTTGGGCTATAAT
GACACCAACAATATCGATGATATCCGCCAAGCCATTGCCGATAGCGGCGCTGACTGGCTGACCATTCATGCCCGCACCAA
GACTCAAGGCTATAAGCCGCCTGCTTATTGGGAGAAGATTGTGGCGTTTACTGAGCTACCGATACCCATTATTGCCAATG
GCGAGATTTGGAATGCGCAGCACGCGCATCAGTGTATTGCGCAGTCTCATACGTCACATGTGATGCTCGGCCGCGGTGCC
GTGACCCGCCCAGATTTAGTGGCCCAAATTGATAACCCAGAAAGCGTCATTGAGTGGCAACATCTGATTGCACATCAAAT
TGCCTTTTTGCAAGGGGAGGCCAAGAATGACTTGGTGCTTATTGGCCGTTACAAACAGTGGTTAGGTATGCTCAGTAAAG
GCTATGTGGAGGCCAAACAGCTGTGGGAGCAGATTAAACGTGAGCGCAATAAGCAGACCATTGTTGATTTGCTACAAGCA
CAGTTAGGCTAA

Upstream 100 bases:

>100_bases
CTCTGGATTAAGAGATGGCAAAAAAGTTATCTATAATAAGTCGCCATAAATAAAACAGATACGTTATCATGCCCTCTTTT
AGCCCATACGTGTGACCGCC

Downstream 100 bases:

>100_bases
AAACAGCTCTGGTGTTTAACAAGCTTATTAACAACGCAACCACTTAGCGTAACAATCAGACCGCCATACGCAGTGCTAGA
TAGAACAGCATAAAGCAGCA

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MCFSDQFFSSPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRITHHVLPEHVFYRFVPELLQSGTTDSGTPI
HVQLLGSEPKLMGANALAAAELGAPAIDLNFGCPAKTVNNHRGGSVLLDEPRLMRDIISAVREAVPAHIPVSAKIRLGYN
DTNNIDDIRQAIADSGADWLTIHARTKTQGYKPPAYWEKIVAFTELPIPIIANGEIWNAQHAHQCIAQSHTSHVMLGRGA
VTRPDLVAQIDNPESVIEWQHLIAHQIAFLQGEAKNDLVLIGRYKQWLGMLSKGYVEAKQLWEQIKRERNKQTIVDLLQA
QLG

Sequences:

>Translated_323_residues
MCFSDQFFSSPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRITHHVLPEHVFYRFVPELLQSGTTDSGTPI
HVQLLGSEPKLMGANALAAAELGAPAIDLNFGCPAKTVNNHRGGSVLLDEPRLMRDIISAVREAVPAHIPVSAKIRLGYN
DTNNIDDIRQAIADSGADWLTIHARTKTQGYKPPAYWEKIVAFTELPIPIIANGEIWNAQHAHQCIAQSHTSHVMLGRGA
VTRPDLVAQIDNPESVIEWQHLIAHQIAFLQGEAKNDLVLIGRYKQWLGMLSKGYVEAKQLWEQIKRERNKQTIVDLLQA
QLG
>Mature_323_residues
MCFSDQFFSSPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRITHHVLPEHVFYRFVPELLQSGTTDSGTPI
HVQLLGSEPKLMGANALAAAELGAPAIDLNFGCPAKTVNNHRGGSVLLDEPRLMRDIISAVREAVPAHIPVSAKIRLGYN
DTNNIDDIRQAIADSGADWLTIHARTKTQGYKPPAYWEKIVAFTELPIPIIANGEIWNAQHAHQCIAQSHTSHVMLGRGA
VTRPDLVAQIDNPESVIEWQHLIAHQIAFLQGEAKNDLVLIGRYKQWLGMLSKGYVEAKQLWEQIKRERNKQTIVDLLQA
QLG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=26.3598326359833, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI40807366, Length=200, Percent_Identity=31, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239788483, Length=173, Percent_Identity=27.7456647398844, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI239788462, Length=173, Percent_Identity=27.7456647398844, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788462, Length=311, Percent_Identity=41.8006430868167, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789660, Length=239, Percent_Identity=33.0543933054393, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI145693211, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=181, Percent_Identity=29.8342541436464, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=179, Percent_Identity=26.2569832402235, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=189, Percent_Identity=30.6878306878307, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323437, Length=245, Percent_Identity=24.8979591836735, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585320, Length=195, Percent_Identity=27.1794871794872, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580595, Length=196, Percent_Identity=27.5510204081633, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=196, Percent_Identity=27.5510204081633, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921524, Length=177, Percent_Identity=28.2485875706215, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35993; Mature: 35993

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00141 ASP_PROTEASE ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFSDQFFSSPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRITHHVLPEHV
CCCCCHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FYRFVPELLQSGTTDSGTPIHVQLLGSEPKLMGANALAAAELGAPAIDLNFGCPAKTVNN
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCC
HRGGSVLLDEPRLMRDIISAVREAVPAHIPVSAKIRLGYNDTNNIDDIRQAIADSGADWL
CCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEE
TIHARTKTQGYKPPAYWEKIVAFTELPIPIIANGEIWNAQHAHQCIAQSHTSHVMLGRGA
EEEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCC
VTRPDLVAQIDNPESVIEWQHLIAHQIAFLQGEAKNDLVLIGRYKQWLGMLSKGYVEAKQ
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCHHHHHH
LWEQIKRERNKQTIVDLLQAQLG
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCFSDQFFSSPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRITHHVLPEHV
CCCCCHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FYRFVPELLQSGTTDSGTPIHVQLLGSEPKLMGANALAAAELGAPAIDLNFGCPAKTVNN
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCC
HRGGSVLLDEPRLMRDIISAVREAVPAHIPVSAKIRLGYNDTNNIDDIRQAIADSGADWL
CCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEE
TIHARTKTQGYKPPAYWEKIVAFTELPIPIIANGEIWNAQHAHQCIAQSHTSHVMLGRGA
EEEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCC
VTRPDLVAQIDNPESVIEWQHLIAHQIAFLQGEAKNDLVLIGRYKQWLGMLSKGYVEAKQ
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCHHHHHH
LWEQIKRERNKQTIVDLLQAQLG
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]