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The map label for this gene is bioB

Identifier: 148653984

GI number: 148653984

Start: 2724059

End: 2725162

Strand: Direct

Name: bioB

Synonym: PsycPRwf_2187

Alternate gene names: 148653984

Gene position: 2724059-2725162 (Clockwise)

Preceding gene: 148653983

Following gene: 148653985

Centisome position: 91.44

GC content: 50.0

Gene sequence:

>1104_bases
ATGAGCTTTTTGGCCACCTTAAAAGAACCAGGCACCAGCGTTTGCTTAAAAAATGAGCCGGCTCAAAGCCCGAGCCTACA
TGACCATGCCCCCTCTACGGCTGCTATTAGCCGCGAATATATCGCCAATCTTTTTGATATGCCGTTTATGGATCTGCTGC
TAAAAGCTCAAGGTGTGCACCGTCAGCACTTCACTGCCAATGCCGTACAAATCAGTACCCTGTTGTCGATTAAGACCGGC
AACTGTCCTGAGGACTGTGGCTATTGCTCTCAATCTGGCCACCACCGAGATACTACGGGGCTACAGGCCGAAAAACGTCT
TGAGATTGATAAAGTCATCGCCGCCGCTAAGCGAGCTAAGGCTTCGGGCTCTTCACGCTTTTGCATGGGTGCAGCATGGA
AGCATCCGAGTGCCAAAGATATGCCTTATGTTGCTGAGCTGATTAAAGAGGTTAAGGCTTTAGGACTTGAGACTTGCATG
ACGTTAGGTATGCTCACCGCTGAGCAGTCAGAGCAACTGGCTGAAGCTGGACTTGATTATTACAACCATAACCTAGATAC
CTCTCGGCGCTATTATGATGAGGTGGTGAGTACGCGTAGCTATGATGAGCGCTTGCAAACCATCAACCACGTACGCAAGT
CAGGTATCAATGTCTGTAGTGGCAGTATTGTGGGCATGGGTGAGAGCCGTGAAGACCGTATTGATTGGGTGCTTGAGCTG
GCCAGTATGCCGCTACCCCCAGAGTCTATTCCAGTCAACTTATTGGTGCCCATTCAAGGCACGCCTCTTGGTGATAAGGT
GCTTAGTGAAGGCCAGCTGCCGGTGCTAGAGTGGATTCGCACCATCGCGGTGACTCGTATTTGTTGTCCCAGCAGTTATG
TACGGCTATCGGCAGGACGTGAGAGTCTGTCTGATGCCGAGCAGGCTCTAGCCTTCATGGCAGGGGCTAACTCGTTTTTT
TATGGCGATAAACTGCTCACCACCGGCAATGCCAGTCAATCTAATGATGATAGAATGATGCAGATGCTTGGACTTATCCC
TGAAGCACCTAAGCCGAAGCTTACTGTGATTGATGCGCTAAGTGGTCATCAAAGTCAGCTATAA

Upstream 100 bases:

>100_bases
ACGGTTAAGCGTTTTGTATTTTTTTATCTTGCTAATAAAATGTTACAATTAGGCTAATTTTTTATATTTGACCCTACCCC
AGTTAACAAAGGAATGTTTT

Downstream 100 bases:

>100_bases
TAGGGGTATCAACCCTTAACTGAGTAATGTGATTATGCCTGAGTATTTTAATTGGATAAAAGCGGCGCACGTCATCTCTA
TGGTCTGCTGGTTTGCGGCT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSFLATLKEPGTSVCLKNEPAQSPSLHDHAPSTAAISREYIANLFDMPFMDLLLKAQGVHRQHFTANAVQISTLLSIKTG
NCPEDCGYCSQSGHHRDTTGLQAEKRLEIDKVIAAAKRAKASGSSRFCMGAAWKHPSAKDMPYVAELIKEVKALGLETCM
TLGMLTAEQSEQLAEAGLDYYNHNLDTSRRYYDEVVSTRSYDERLQTINHVRKSGINVCSGSIVGMGESREDRIDWVLEL
ASMPLPPESIPVNLLVPIQGTPLGDKVLSEGQLPVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSFF
YGDKLLTTGNASQSNDDRMMQMLGLIPEAPKPKLTVIDALSGHQSQL

Sequences:

>Translated_367_residues
MSFLATLKEPGTSVCLKNEPAQSPSLHDHAPSTAAISREYIANLFDMPFMDLLLKAQGVHRQHFTANAVQISTLLSIKTG
NCPEDCGYCSQSGHHRDTTGLQAEKRLEIDKVIAAAKRAKASGSSRFCMGAAWKHPSAKDMPYVAELIKEVKALGLETCM
TLGMLTAEQSEQLAEAGLDYYNHNLDTSRRYYDEVVSTRSYDERLQTINHVRKSGINVCSGSIVGMGESREDRIDWVLEL
ASMPLPPESIPVNLLVPIQGTPLGDKVLSEGQLPVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSFF
YGDKLLTTGNASQSNDDRMMQMLGLIPEAPKPKLTVIDALSGHQSQL
>Mature_366_residues
SFLATLKEPGTSVCLKNEPAQSPSLHDHAPSTAAISREYIANLFDMPFMDLLLKAQGVHRQHFTANAVQISTLLSIKTGN
CPEDCGYCSQSGHHRDTTGLQAEKRLEIDKVIAAAKRAKASGSSRFCMGAAWKHPSAKDMPYVAELIKEVKALGLETCMT
LGMLTAEQSEQLAEAGLDYYNHNLDTSRRYYDEVVSTRSYDERLQTINHVRKSGINVCSGSIVGMGESREDRIDWVLELA
SMPLPPESIPVNLLVPIQGTPLGDKVLSEGQLPVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSFFY
GDKLLTTGNASQSNDDRMMQMLGLIPEAPKPKLTVIDALSGHQSQL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=55.5194805194805, Blast_Score=377, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321725, Length=315, Percent_Identity=44.1269841269841, Blast_Score=291, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PSYWF (A5WHI6)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001281077.1
- ProteinModelPortal:   A5WHI6
- SMR:   A5WHI6
- STRING:   A5WHI6
- GeneID:   5205926
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_2187
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2391056
- BioCyc:   PSP56811:PSYCPRWF_2187-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 40105; Mature: 39974

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLATLKEPGTSVCLKNEPAQSPSLHDHAPSTAAISREYIANLFDMPFMDLLLKAQGVH
CCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCC
RQHFTANAVQISTLLSIKTGNCPEDCGYCSQSGHHRDTTGLQAEKRLEIDKVIAAAKRAK
HHHCCCCCEEEEHEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASGSSRFCMGAAWKHPSAKDMPYVAELIKEVKALGLETCMTLGMLTAEQSEQLAEAGLDY
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNHNLDTSRRYYDEVVSTRSYDERLQTINHVRKSGINVCSGSIVGMGESREDRIDWVLEL
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCEEECCCCCHHHHHHHHHH
ASMPLPPESIPVNLLVPIQGTPLGDKVLSEGQLPVLEWIRTIAVTRICCPSSYVRLSAGR
HHCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCH
ESLSDAEQALAFMAGANSFFYGDKLLTTGNASQSNDDRMMQMLGLIPEAPKPKLTVIDAL
HHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECC
SGHQSQL
CCCCCCC
>Mature Secondary Structure 
SFLATLKEPGTSVCLKNEPAQSPSLHDHAPSTAAISREYIANLFDMPFMDLLLKAQGVH
CCHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCC
RQHFTANAVQISTLLSIKTGNCPEDCGYCSQSGHHRDTTGLQAEKRLEIDKVIAAAKRAK
HHHCCCCCEEEEHEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASGSSRFCMGAAWKHPSAKDMPYVAELIKEVKALGLETCMTLGMLTAEQSEQLAEAGLDY
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNHNLDTSRRYYDEVVSTRSYDERLQTINHVRKSGINVCSGSIVGMGESREDRIDWVLEL
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCEEECCCCCHHHHHHHHHH
ASMPLPPESIPVNLLVPIQGTPLGDKVLSEGQLPVLEWIRTIAVTRICCPSSYVRLSAGR
HHCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCH
ESLSDAEQALAFMAGANSFFYGDKLLTTGNASQSNDDRMMQMLGLIPEAPKPKLTVIDAL
HHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECC
SGHQSQL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA