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The map label for this gene is rluE [H]

Identifier: 148653886

GI number: 148653886

Start: 2607796

End: 2608407

Strand: Direct

Name: rluE [H]

Synonym: PsycPRwf_2089

Alternate gene names: 148653886

Gene position: 2607796-2608407 (Clockwise)

Preceding gene: 148653885

Following gene: 148653888

Centisome position: 87.54

GC content: 49.51

Gene sequence:

>612_bases
ATGGCCACCTCAAGTGTTATCTTATTTAACAAACCCTACGGCGTTCAAAGTCAGTTTAGTGATGATGCCAATAACAAGTT
TGCGCCGTTATCCCAGTTTTTCACCGATAAATCGCTCCGAGTGGCCGGACGACTCGATGCGACCAGTGAGGGGCTTTTGC
TGCTGACCTCAGATGGCCAGGTCAATAAAGCCATTACTCATCCGCCCAAAGCCAATGCAGGGCGTAAGCAGGGCAAAACT
TACTTAGTACAGGTAGAGGGCATCCCCTCAAATGAGCAATTAGAGCAACTGCGTCAAGGGGTGCTGCTAAAAGATGGTCA
GACCCTACCCGCTGAGGTAAAACACATTAGTGAAGAGGATTTACCCATGCCACTTTGGCAACGTGATCCACCCATTCGTG
AACGCAAAAGTGTGCCTGACTCATGGCTAATGATGACCATTTATGAGGGTAAAAACCGCCAAGTGCGCCGCATGACCGCT
CATGTTGGCCTGCCCTGCCTACGCCTAATTCGCTGGTCTGCTGCCGGCTTTGAGCTGGGCAATTTAGCGGTAGGCGAGTT
TGTACGGGTACATTTAAATGACAAGCGATTAAAGGAGCTGGGTGTTTTTTAA

Upstream 100 bases:

>100_bases
ACGGCCAAAGGCAGTGGTATAAACCGCGGCACTGATCCTGATTAGGCACCAAAATCGTTGTTTACTCCCTTTTCATTGAT
GTATTTGGAACTTCCCTTTT

Downstream 100 bases:

>100_bases
ATCGTTAAACAACACCCTGGTATAGCCGTGCCCTTTTGGCTCGGCTATACCCTATCAATCACTACCGTATAAAATCTGGT
GTTAACCAGTGCTCAAAGCC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MATSSVILFNKPYGVQSQFSDDANNKFAPLSQFFTDKSLRVAGRLDATSEGLLLLTSDGQVNKAITHPPKANAGRKQGKT
YLVQVEGIPSNEQLEQLRQGVLLKDGQTLPAEVKHISEEDLPMPLWQRDPPIRERKSVPDSWLMMTIYEGKNRQVRRMTA
HVGLPCLRLIRWSAAGFELGNLAVGEFVRVHLNDKRLKELGVF

Sequences:

>Translated_203_residues
MATSSVILFNKPYGVQSQFSDDANNKFAPLSQFFTDKSLRVAGRLDATSEGLLLLTSDGQVNKAITHPPKANAGRKQGKT
YLVQVEGIPSNEQLEQLRQGVLLKDGQTLPAEVKHISEEDLPMPLWQRDPPIRERKSVPDSWLMMTIYEGKNRQVRRMTA
HVGLPCLRLIRWSAAGFELGNLAVGEFVRVHLNDKRLKELGVF
>Mature_202_residues
ATSSVILFNKPYGVQSQFSDDANNKFAPLSQFFTDKSLRVAGRLDATSEGLLLLTSDGQVNKAITHPPKANAGRKQGKTY
LVQVEGIPSNEQLEQLRQGVLLKDGQTLPAEVKHISEEDLPMPLWQRDPPIRERKSVPDSWLMMTIYEGKNRQVRRMTAH
VGLPCLRLIRWSAAGFELGNLAVGEFVRVHLNDKRLKELGVF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=54.0106951871658, Blast_Score=193, Evalue=6e-51,
Organism=Escherichia coli, GI1788510, Length=186, Percent_Identity=31.7204301075269, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787524, Length=129, Percent_Identity=32.5581395348837, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 22764; Mature: 22633

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSSVILFNKPYGVQSQFSDDANNKFAPLSQFFTDKSLRVAGRLDATSEGLLLLTSDGQ
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEECCCC
VNKAITHPPKANAGRKQGKTYLVQVEGIPSNEQLEQLRQGVLLKDGQTLPAEVKHISEED
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEECCCCCCHHHHHCCCCC
LPMPLWQRDPPIRERKSVPDSWLMMTIYEGKNRQVRRMTAHVGLPCLRLIRWSAAGFELG
CCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCCCC
NLAVGEFVRVHLNDKRLKELGVF
CEEECEEEEEEECHHHHHHHCCC
>Mature Secondary Structure 
ATSSVILFNKPYGVQSQFSDDANNKFAPLSQFFTDKSLRVAGRLDATSEGLLLLTSDGQ
CCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEECCCC
VNKAITHPPKANAGRKQGKTYLVQVEGIPSNEQLEQLRQGVLLKDGQTLPAEVKHISEED
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEECCCCCCHHHHHCCCCC
LPMPLWQRDPPIRERKSVPDSWLMMTIYEGKNRQVRRMTAHVGLPCLRLIRWSAAGFELG
CCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCCCC
NLAVGEFVRVHLNDKRLKELGVF
CEEECEEEEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]