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The map label for this gene is wax-dgaT [H]

Identifier: 148653637

GI number: 148653637

Start: 2288422

End: 2289807

Strand: Direct

Name: wax-dgaT [H]

Synonym: PsycPRwf_1840

Alternate gene names: 148653637

Gene position: 2288422-2289807 (Clockwise)

Preceding gene: 148653636

Following gene: 148653638

Centisome position: 76.82

GC content: 44.52

Gene sequence:

>1386_bases
ATGCGTCTATTATCATTGGTAGATTTACTGTTTTTATTGGTTGAAAAAAGAAAGCAGCCTATGCACGTCGGTGGGCTGTT
TTTATTCGAGGTTCCCAAAGATGCCCAAGAGGATTTTGTGCTGTCTTTGGTGCAGCAAATGCAAAACTCTACAGTCCCCC
CTACTTTTCCGTTTAATCAGGTGTTGCACAACTTAACGTACTGGAAAAACGATGACGAGTTTGATGTTGAGCATCATTTT
CGCCATATTGCGCTGCCTAAGCCTGCCCGAGTGCGCGAGCTATTATCTTATATCTCAAAAGAGCATGGTCGCCTGCTAGA
TAGGGCGCGTCCGCTATGGGAATGCCACATTATTGAGGGTATTGAGCCGGAGAGTGACGGTCGCCCCAAAAGATTTGGGC
TTTATTTTAAAATTCACCACTCGTTGGTAGATGGCATTGCGGCCATGCGCTTGGTTCAAAAGTCGTTATCACAGTCACCC
AATGAGACCATCACCTTACCACTTTGGGCGTTAATGATGCGCCACCGCCATCAAATTGAGTCGGTGATACCAAAAGAGCG
TGGCATTGGCAGTATCATCAAAGACCAATTACAAACCGCCAAGCCGGTGATGGGCGAGCTACTTAGTGCTCTAAAAATCG
ATAAAGACAGTAGCTATGTGCGCACCACTCAGGCGCCGCCCAGCATCTTAAACCAAAGCATTTCAAGCTCACGCCGCTTT
ATTGCTGACTCCTATGACATTGCTCGCTTTAAAAGAGTGGCAAATAATTTGGGGGTGAGTATTAATGATGTGGTACTAAC
TGTATGTTCAGGGGCATTAAGACGCTATTTATTATCCATCAATGAGCTACCAAAGGATCCGTTAATCGCATGGGTACCCT
ACTCTATGCGTAAAGATGAGACAGTCGCGGGCAATCAGTTTGCCTTTATCTTATGTAATTTGGGCACGCATTTGGCCAGT
CCTCTAGAGCGCTTAAAGCTTGTCCATAACAGCATGAATGATGGTAAAAAGCGCTTTGGTCGTATGTCACAAGCGGAGGT
TGTCAACTATAGCTTGGTCGCTTACAGCTGGGAGTTTGTGAATATTTTTACCAATGCTTATCCCAAAAAACATGCGTTTA
ACTTAATTATTTCCAATGTTCCTGGCTCACAAAAAAAGCTATACTGGAACGGTGCGGAATTAAGAGCCTTGTATCCAGCT
TCTATCTTATTTAATGGTCAGGCAATGAACATTACTTTAGCCAGTTATTTGGACCGCATCGAGTTTGGTATCGTGGCGTG
TAGTAAAACTCTGCCCAAGATTCAAAGCCTGCTAAAGATGCTAGAAGATGAGTTGGGCCAACTTGAAACCATCTGTACCG
AAAGAGAGTTGGGCATTCGCGAATAA

Upstream 100 bases:

>100_bases
TTTACTATCTGTAGCTTAAGCGTGTTTTAATGAATTATTATTCTGTGTACAAGCGGCTTTATGTGACCGTCTATTGTATT
TGATACAGCAAGGGAATGTC

Downstream 100 bases:

>100_bases
ACCGCTGCAGCCCACTTGCCTTGTAGCTTACTGTTAGATGATGACTAAGAACATCAAGACAAATGGCGATAAGGCAGGGC
TGGCTTATCGGGTTTTACTA

Product: acyltransferase ws/dgat/mgat family protein

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRLLSLVDLLFLLVEKRKQPMHVGGLFLFEVPKDAQEDFVLSLVQQMQNSTVPPTFPFNQVLHNLTYWKNDDEFDVEHHF
RHIALPKPARVRELLSYISKEHGRLLDRARPLWECHIIEGIEPESDGRPKRFGLYFKIHHSLVDGIAAMRLVQKSLSQSP
NETITLPLWALMMRHRHQIESVIPKERGIGSIIKDQLQTAKPVMGELLSALKIDKDSSYVRTTQAPPSILNQSISSSRRF
IADSYDIARFKRVANNLGVSINDVVLTVCSGALRRYLLSINELPKDPLIAWVPYSMRKDETVAGNQFAFILCNLGTHLAS
PLERLKLVHNSMNDGKKRFGRMSQAEVVNYSLVAYSWEFVNIFTNAYPKKHAFNLIISNVPGSQKKLYWNGAELRALYPA
SILFNGQAMNITLASYLDRIEFGIVACSKTLPKIQSLLKMLEDELGQLETICTERELGIRE

Sequences:

>Translated_461_residues
MRLLSLVDLLFLLVEKRKQPMHVGGLFLFEVPKDAQEDFVLSLVQQMQNSTVPPTFPFNQVLHNLTYWKNDDEFDVEHHF
RHIALPKPARVRELLSYISKEHGRLLDRARPLWECHIIEGIEPESDGRPKRFGLYFKIHHSLVDGIAAMRLVQKSLSQSP
NETITLPLWALMMRHRHQIESVIPKERGIGSIIKDQLQTAKPVMGELLSALKIDKDSSYVRTTQAPPSILNQSISSSRRF
IADSYDIARFKRVANNLGVSINDVVLTVCSGALRRYLLSINELPKDPLIAWVPYSMRKDETVAGNQFAFILCNLGTHLAS
PLERLKLVHNSMNDGKKRFGRMSQAEVVNYSLVAYSWEFVNIFTNAYPKKHAFNLIISNVPGSQKKLYWNGAELRALYPA
SILFNGQAMNITLASYLDRIEFGIVACSKTLPKIQSLLKMLEDELGQLETICTERELGIRE
>Mature_461_residues
MRLLSLVDLLFLLVEKRKQPMHVGGLFLFEVPKDAQEDFVLSLVQQMQNSTVPPTFPFNQVLHNLTYWKNDDEFDVEHHF
RHIALPKPARVRELLSYISKEHGRLLDRARPLWECHIIEGIEPESDGRPKRFGLYFKIHHSLVDGIAAMRLVQKSLSQSP
NETITLPLWALMMRHRHQIESVIPKERGIGSIIKDQLQTAKPVMGELLSALKIDKDSSYVRTTQAPPSILNQSISSSRRF
IADSYDIARFKRVANNLGVSINDVVLTVCSGALRRYLLSINELPKDPLIAWVPYSMRKDETVAGNQFAFILCNLGTHLAS
PLERLKLVHNSMNDGKKRFGRMSQAEVVNYSLVAYSWEFVNIFTNAYPKKHAFNLIISNVPGSQKKLYWNGAELRALYPA
SILFNGQAMNITLASYLDRIEFGIVACSKTLPKIQSLLKMLEDELGQLETICTERELGIRE

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 52564; Mature: 52564

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLSLVDLLFLLVEKRKQPMHVGGLFLFEVPKDAQEDFVLSLVQQMQNSTVPPTFPFNQ
CHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
VLHNLTYWKNDDEFDVEHHFRHIALPKPARVRELLSYISKEHGRLLDRARPLWECHIIEG
HHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
IEPESDGRPKRFGLYFKIHHSLVDGIAAMRLVQKSLSQSPNETITLPLWALMMRHRHQIE
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SVIPKERGIGSIIKDQLQTAKPVMGELLSALKIDKDSSYVRTTQAPPSILNQSISSSRRF
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHH
IADSYDIARFKRVANNLGVSINDVVLTVCSGALRRYLLSINELPKDPLIAWVPYSMRKDE
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
TVAGNQFAFILCNLGTHLASPLERLKLVHNSMNDGKKRFGRMSQAEVVNYSLVAYSWEFV
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHEEEHHEEEHHHH
NIFTNAYPKKHAFNLIISNVPGSQKKLYWNGAELRALYPASILFNGQAMNITLASYLDRI
HHHHCCCCCHHHHEEEEECCCCCCCEEEECCCCEEEECCCEEEECCCEEEEEHHHHHHHH
EFGIVACSKTLPKIQSLLKMLEDELGQLETICTERELGIRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLLSLVDLLFLLVEKRKQPMHVGGLFLFEVPKDAQEDFVLSLVQQMQNSTVPPTFPFNQ
CHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
VLHNLTYWKNDDEFDVEHHFRHIALPKPARVRELLSYISKEHGRLLDRARPLWECHIIEG
HHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
IEPESDGRPKRFGLYFKIHHSLVDGIAAMRLVQKSLSQSPNETITLPLWALMMRHRHQIE
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SVIPKERGIGSIIKDQLQTAKPVMGELLSALKIDKDSSYVRTTQAPPSILNQSISSSRRF
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHH
IADSYDIARFKRVANNLGVSINDVVLTVCSGALRRYLLSINELPKDPLIAWVPYSMRKDE
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
TVAGNQFAFILCNLGTHLASPLERLKLVHNSMNDGKKRFGRMSQAEVVNYSLVAYSWEFV
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHEEEHHEEEHHHH
NIFTNAYPKKHAFNLIISNVPGSQKKLYWNGAELRALYPASILFNGQAMNITLASYLDRI
HHHHCCCCCHHHHEEEEECCCCCCCEEEECCCCEEEECCCEEEECCCEEEEEHHHHHHHH
EFGIVACSKTLPKIQSLLKMLEDELGQLETICTERELGIRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715 [H]