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The map label for this gene is thiG [H]

Identifier: 148653636

GI number: 148653636

Start: 2287377

End: 2288225

Strand: Direct

Name: thiG [H]

Synonym: PsycPRwf_1839

Alternate gene names: 148653636

Gene position: 2287377-2288225 (Clockwise)

Preceding gene: 148653631

Following gene: 148653637

Centisome position: 76.78

GC content: 47.94

Gene sequence:

>849_bases
ATGACCCTGGATTCTACATTACCTCACAGTACCACAGATTTTGACACTGACGTGCTACAGCAAGATAAGTTCACTATTGG
TAGCCGCACCTTTTACTCAAGGCTGTTGGTGGGTACGGGCAAATATAAAGACTTAACCCAGACCGCTCAAGCAATTCAGG
CTTCTGGTGCTGAGATTGTCACTGTGGCCATTCGTCGCACCAATATCGGTCAACATAAAGACCAGCCTAACCTGTTAGAG
GTCATCTCACCAGATAAATATACGATTTTGCCCAATACAGCCGGCTGCTTCGATGCTGAAACTGCCATCCGTACTTGCCA
GCTATCTCGTGAGTTATTAGATGGTCACAACCTCGTAAAATTAGAAGTTCTGGGAGATCAAGATACGCTTTATCCCAATG
TCACTCAGACCTTAACCGCAGCCAAAAAGCTCATTGACGATGGTTTTGAGGTCATGGTTTACACCTCAGATGACCCTATT
GTCGCCCAAGAATTAGCCGCTATGGGCTGTGTGGCCATTATGCCTCTAGGCAGCCTAATTGGGTCGGGACTTGGCCTATT
AAACCCACATACGCTAAGTTTGATTGTCGAAAAAACCAAAGCTTATAATCTGCCAGTTCTGGTCGATGCTGGCGTGGGCG
CCGCCTCTGATGCCGCGATTGCCATGGAGCTTGGCTGCGATGGGGTATTAATGAACTCTGCCATTGCCAATGCCCAAAAC
CCAGTAATGATGGCGCATGCTATGAAACATGCTATTTGGGCTGGGCGTGCCTCTTATTTGGCAGGCCGTATGCCTAAGCG
TAAAATGGCGGTGCCAAGCTCCCCACAAACCGGCTATTTCTTTCAATAA

Upstream 100 bases:

>100_bases
CGGTATCGGCTTATAATTTTAAATCGATAACCACCACCACCTGACAAGATTGACCTCCCTCTTAACCCTAAAAACCAAGT
TCAAAGACAAGGCAACTGCA

Downstream 100 bases:

>100_bases
TCTGATAGAGTTTGGGTGAATGTAAAAACACTATGAAAGTCAAATCTCTTAATGACCTAATGACTCATAAATAGATTTCA
TTGACTTAATTTTTGATTTA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTLDSTLPHSTTDFDTDVLQQDKFTIGSRTFYSRLLVGTGKYKDLTQTAQAIQASGAEIVTVAIRRTNIGQHKDQPNLLE
VISPDKYTILPNTAGCFDAETAIRTCQLSRELLDGHNLVKLEVLGDQDTLYPNVTQTLTAAKKLIDDGFEVMVYTSDDPI
VAQELAAMGCVAIMPLGSLIGSGLGLLNPHTLSLIVEKTKAYNLPVLVDAGVGAASDAAIAMELGCDGVLMNSAIANAQN
PVMMAHAMKHAIWAGRASYLAGRMPKRKMAVPSSPQTGYFFQ

Sequences:

>Translated_282_residues
MTLDSTLPHSTTDFDTDVLQQDKFTIGSRTFYSRLLVGTGKYKDLTQTAQAIQASGAEIVTVAIRRTNIGQHKDQPNLLE
VISPDKYTILPNTAGCFDAETAIRTCQLSRELLDGHNLVKLEVLGDQDTLYPNVTQTLTAAKKLIDDGFEVMVYTSDDPI
VAQELAAMGCVAIMPLGSLIGSGLGLLNPHTLSLIVEKTKAYNLPVLVDAGVGAASDAAIAMELGCDGVLMNSAIANAQN
PVMMAHAMKHAIWAGRASYLAGRMPKRKMAVPSSPQTGYFFQ
>Mature_281_residues
TLDSTLPHSTTDFDTDVLQQDKFTIGSRTFYSRLLVGTGKYKDLTQTAQAIQASGAEIVTVAIRRTNIGQHKDQPNLLEV
ISPDKYTILPNTAGCFDAETAIRTCQLSRELLDGHNLVKLEVLGDQDTLYPNVTQTLTAAKKLIDDGFEVMVYTSDDPIV
AQELAAMGCVAIMPLGSLIGSGLGLLNPHTLSLIVEKTKAYNLPVLVDAGVGAASDAAIAMELGCDGVLMNSAIANAQNP
VMMAHAMKHAIWAGRASYLAGRMPKRKMAVPSSPQTGYFFQ

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=255, Percent_Identity=41.5686274509804, Blast_Score=184, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 30260; Mature: 30129

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDSTLPHSTTDFDTDVLQQDKFTIGSRTFYSRLLVGTGKYKDLTQTAQAIQASGAEIV
CCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
TVAIRRTNIGQHKDQPNLLEVISPDKYTILPNTAGCFDAETAIRTCQLSRELLDGHNLVK
EEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LEVLGDQDTLYPNVTQTLTAAKKLIDDGFEVMVYTSDDPIVAQELAAMGCVAIMPLGSLI
EEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GSGLGLLNPHTLSLIVEKTKAYNLPVLVDAGVGAASDAAIAMELGCDGVLMNSAIANAQN
HCCCCCCCCCEEEEEEEHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCC
PVMMAHAMKHAIWAGRASYLAGRMPKRKMAVPSSPQTGYFFQ
CHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLDSTLPHSTTDFDTDVLQQDKFTIGSRTFYSRLLVGTGKYKDLTQTAQAIQASGAEIV
CCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
TVAIRRTNIGQHKDQPNLLEVISPDKYTILPNTAGCFDAETAIRTCQLSRELLDGHNLVK
EEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LEVLGDQDTLYPNVTQTLTAAKKLIDDGFEVMVYTSDDPIVAQELAAMGCVAIMPLGSLI
EEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GSGLGLLNPHTLSLIVEKTKAYNLPVLVDAGVGAASDAAIAMELGCDGVLMNSAIANAQN
HCCCCCCCCCEEEEEEEHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCC
PVMMAHAMKHAIWAGRASYLAGRMPKRKMAVPSSPQTGYFFQ
CHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA