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The map label for this gene is mii [H]

Identifier: 148653006

GI number: 148653006

Start: 1468484

End: 1469737

Strand: Direct

Name: mii [H]

Synonym: PsycPRwf_1200

Alternate gene names: 148653006

Gene position: 1468484-1469737 (Clockwise)

Preceding gene: 148653005

Following gene: 148653007

Centisome position: 49.29

GC content: 48.8

Gene sequence:

>1254_bases
ATGACCCAATCTAATACACCTTTTGCCCCCCAAATTTCAGTACCTGCCACCTATATGCGTGGCGGCACCTCCAAAGGCAC
GTTTTTTAAATTAAGTGATTTGCCTGAGCGTTGCCAACAACCTGGCGAGGCTCGCGATAACTTTTTAATGCGCGTCATTG
GCAGCCCCGATCCTTATGGCAAGCAAATCGATGGCTTGGGTAATGGGTCATCTAGTACCTCAAAGACGGTTATTCTCTCT
AAACCTGATCAAGCCAAGTCTGAAAAAGATAGCCACGATGTGAATTACCTGTTTGGGCAAGTGAATATTGCCAAGCCCAT
GATTGATTGGGGGGGTAATTGTGGTAATTTAACCGCTGCAGTGGGCGCTTGTGCCATCAATATGGGTCTGGTCGATGCTG
ATAAAGTAGCTGCTAGTGCCAAGAGCGGTATTTGTGAAGTGCGTATTTGGCAGCAGAATATTAGCAAAACTATCATTGCC
CATGTGCCGGTCTACACTGATGAGCAAGGACAAGTGCAGGTACAAGAAACCGGCGATTTTGAATTAGATGGGGTTACCTT
CCCTGCCGCAGAGGTAAAAATTGAATTTATTGATCCGGTTGACGCGTCAAGCGCTATGTTCCCAACAGGCCATCTGGTTG
ACGACTTTGATGTCTCCGGGTGCGGACTGGATAAAGACTATGTTCAAGCGACCTTTATCAATGCCGGCATCCCCACCATA
TTTATGAAAGCTGAGGACTTAGGCTTTAATGGTACTGAGCTTCAGTCTGATATTAATGGCAATCAAGAGCTGCTTGATAA
GCTTGAGACTATCCGCGCCAAAGGCGGTGTGGCTATGGGCTTATTTCAGGATGTAAGTGACGCACAAAAAAGCCAGCATA
TCCCTAAGATTGCTTGGGTGGCACCTGCTCAGTCTTACACTGCCTCAAGTGGTAAGGTGGTCGATAAAGACGACATTGAC
TTGGTGGTACGTGCCATGAGTATGGGGCAGCTGCACCATGCGATGATGGGTACAGCGGCGGTGGCCATTGCCGCTGCTGC
TACCACGCAAGGTACATTGGTTAACCAATCAGCTGGCAATGGCTCAGCAACACAGCTGTCTGAAGTACGCTTTGGTCATC
CCTCTGGTACACTGCTTGTGGGCGGTAAAACTGAGCTGGTAGATGGGCGCTGGCAAGCCAAAAAAGTGTCTATGAGCCGC
TCTGCTCGCCGAATTATGGTCGGGCAAGTGTTTGTGCCTGGTGACTGCTTTTAG

Upstream 100 bases:

>100_bases
TTGCTGATAAATCGCGTCTTAATTAAGCGCATAAGCCACCTTTTATTGTGCTTGTGCGCTTTTTTAATAAAAAATAATAT
CCCCCTCATAAGGATTTGTC

Downstream 100 bases:

>100_bases
AGTTTTTGGGATGACAATTGGCCTCTATAATGACCTTGGTGAGCGGATATAAAGAGTCATCATAGGGCTTTGCTTTCAAA
AATATTACAATGGCGCCATA

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTQSNTPFAPQISVPATYMRGGTSKGTFFKLSDLPERCQQPGEARDNFLMRVIGSPDPYGKQIDGLGNGSSSTSKTVILS
KPDQAKSEKDSHDVNYLFGQVNIAKPMIDWGGNCGNLTAAVGACAINMGLVDADKVAASAKSGICEVRIWQQNISKTIIA
HVPVYTDEQGQVQVQETGDFELDGVTFPAAEVKIEFIDPVDASSAMFPTGHLVDDFDVSGCGLDKDYVQATFINAGIPTI
FMKAEDLGFNGTELQSDINGNQELLDKLETIRAKGGVAMGLFQDVSDAQKSQHIPKIAWVAPAQSYTASSGKVVDKDDID
LVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNQSAGNGSATQLSEVRFGHPSGTLLVGGKTELVDGRWQAKKVSMSR
SARRIMVGQVFVPGDCF

Sequences:

>Translated_417_residues
MTQSNTPFAPQISVPATYMRGGTSKGTFFKLSDLPERCQQPGEARDNFLMRVIGSPDPYGKQIDGLGNGSSSTSKTVILS
KPDQAKSEKDSHDVNYLFGQVNIAKPMIDWGGNCGNLTAAVGACAINMGLVDADKVAASAKSGICEVRIWQQNISKTIIA
HVPVYTDEQGQVQVQETGDFELDGVTFPAAEVKIEFIDPVDASSAMFPTGHLVDDFDVSGCGLDKDYVQATFINAGIPTI
FMKAEDLGFNGTELQSDINGNQELLDKLETIRAKGGVAMGLFQDVSDAQKSQHIPKIAWVAPAQSYTASSGKVVDKDDID
LVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNQSAGNGSATQLSEVRFGHPSGTLLVGGKTELVDGRWQAKKVSMSR
SARRIMVGQVFVPGDCF
>Mature_416_residues
TQSNTPFAPQISVPATYMRGGTSKGTFFKLSDLPERCQQPGEARDNFLMRVIGSPDPYGKQIDGLGNGSSSTSKTVILSK
PDQAKSEKDSHDVNYLFGQVNIAKPMIDWGGNCGNLTAAVGACAINMGLVDADKVAASAKSGICEVRIWQQNISKTIIAH
VPVYTDEQGQVQVQETGDFELDGVTFPAAEVKIEFIDPVDASSAMFPTGHLVDDFDVSGCGLDKDYVQATFINAGIPTIF
MKAEDLGFNGTELQSDINGNQELLDKLETIRAKGGVAMGLFQDVSDAQKSQHIPKIAWVAPAQSYTASSGKVVDKDDIDL
VVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNQSAGNGSATQLSEVRFGHPSGTLLVGGKTELVDGRWQAKKVSMSRS
ARRIMVGQVFVPGDCF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=404, Percent_Identity=32.9207920792079, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 44303; Mature: 44172

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSNTPFAPQISVPATYMRGGTSKGTFFKLSDLPERCQQPGEARDNFLMRVIGSPDPYG
CCCCCCCCCCEECCCHHHHCCCCCCCCEEEECCCHHHHCCCCCCCCCEEEEEECCCCCCC
KQIDGLGNGSSSTSKTVILSKPDQAKSEKDSHDVNYLFGQVNIAKPMIDWGGNCGNLTAA
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCHHHH
VGACAINMGLVDADKVAASAKSGICEVRIWQQNISKTIIAHVPVYTDEQGQVQVQETGDF
HHHHHEECCCCCHHHHHHHHHCCEEEEEEEHHCCCCEEEEEECEEECCCCCEEEEECCCE
ELDGVTFPAAEVKIEFIDPVDASSAMFPTGHLVDDFDVSGCGLDKDYVQATFINAGIPTI
EECCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCEE
FMKAEDLGFNGTELQSDINGNQELLDKLETIRAKGGVAMGLFQDVSDAQKSQHIPKIAWV
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEEE
APAQSYTASSGKVVDKDDIDLVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNQSAGN
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GSATQLSEVRFGHPSGTLLVGGKTELVDGRWQAKKVSMSRSARRIMVGQVFVPGDCF
CCCHHHHHEECCCCCCEEEECCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TQSNTPFAPQISVPATYMRGGTSKGTFFKLSDLPERCQQPGEARDNFLMRVIGSPDPYG
CCCCCCCCCEECCCHHHHCCCCCCCCEEEECCCHHHHCCCCCCCCCEEEEEECCCCCCC
KQIDGLGNGSSSTSKTVILSKPDQAKSEKDSHDVNYLFGQVNIAKPMIDWGGNCGNLTAA
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCHHHH
VGACAINMGLVDADKVAASAKSGICEVRIWQQNISKTIIAHVPVYTDEQGQVQVQETGDF
HHHHHEECCCCCHHHHHHHHHCCEEEEEEEHHCCCCEEEEEECEEECCCCCEEEEECCCE
ELDGVTFPAAEVKIEFIDPVDASSAMFPTGHLVDDFDVSGCGLDKDYVQATFINAGIPTI
EECCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCEE
FMKAEDLGFNGTELQSDINGNQELLDKLETIRAKGGVAMGLFQDVSDAQKSQHIPKIAWV
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEEE
APAQSYTASSGKVVDKDDIDLVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNQSAGN
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GSATQLSEVRFGHPSGTLLVGGKTELVDGRWQAKKVSMSRSARRIMVGQVFVPGDCF
CCCHHHHHEECCCCCCEEEECCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA