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The map label for this gene is cysU [H]

Identifier: 148652940

GI number: 148652940

Start: 1389590

End: 1390471

Strand: Direct

Name: cysU [H]

Synonym: PsycPRwf_1134

Alternate gene names: 148652940

Gene position: 1389590-1390471 (Clockwise)

Preceding gene: 148652939

Following gene: 148652941

Centisome position: 46.65

GC content: 49.43

Gene sequence:

>882_bases
ATGAACCAGTCAGCCCCATCGACAGCTCCTATCCGCAAGCGCTCTTGGCTTACCCGATTGCGTCAGCGCAACGTGTTACC
CGGTTTTGGCTTGTCTATGGGTATCACCGTATTGAGCTTATCGTTATTGGTTATTCTACCCTTTGCGATGATGGCTTATA
CCACCACCCAAATGGGGCTAAGTGGTTTTATCGAGGCCATCAGTCAGCTTCAGGTGAAGGCTGCGATTATTTTGTCACTG
AAGATGTCGTTTTATGCCATGTTGACCAATATGGTGTTCGGTACTTTGGTGGCGTGGGTATTGGTGCGTTATGACTTCAT
AGGTAAGTCGCTGATCAATGCCTTGGTGGATCTGCCTTTTGCTTTGCCCACGGCGGTGACTGGTATTTCACTGGCCACCT
TGTATGCGCCCAATGGCTTAATCGGTCAGTTCTTTGACAAGGTGGGTATTCAAGTTGCCTTTACCCCCATTGGTATTTGG
CTGGCCTTGGTGGTGGTAAGCTTTCCATTTATCGTACGTGCTGTGCAGCCGGTATTAGAGGAGCTGTCAGTTGAGTATGA
AGAAGCGGCCGCGGTACTGGGGGCCAATCGCTTGACCACCTTTCGTACGGTGATACTGCCAGAGCTGTTTCCTGCCTTAT
TGATGGGTGCAGGCATGATGTTTGCACGCGCCACCGGAGAGTATGGCTCGGTTATCTTTATTGCTGGTAATATCCCGATG
CAGTCTGAGATTTTACCGCTGATTATTATCAGTAAGCTTGAGCAATTCGATATCCAAGGGGCATCCGCGGTGGCGTTATT
TATGCTCATGATCTCCTTTGTCATCTTATTGGCCATCAACCTACTACAGTGGCGTTTGTCTCGCCGTGTCGGGGCTAAGT
AA

Upstream 100 bases:

>100_bases
GATAGCCTTATTCATCATTAAACTTAATAACCTTGCCCTGTTAGCATGAGCAGCACCATACAAAAGCATGACCCATTTTC
ATCAATGCAGTGAGACCTTC

Downstream 100 bases:

>100_bases
GCCACTCTAGGCCAAGTTATAGGCCAAGTTAGGCCAAGTTATGGGTTAAGTTGTGAGTCAAGTTCAGCCTGCTTTATACA
GTACATTCGGATAATTAGGA

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNQSAPSTAPIRKRSWLTRLRQRNVLPGFGLSMGITVLSLSLLVILPFAMMAYTTTQMGLSGFIEAISQLQVKAAIILSL
KMSFYAMLTNMVFGTLVAWVLVRYDFIGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQFFDKVGIQVAFTPIGIW
LALVVVSFPFIVRAVQPVLEELSVEYEEAAAVLGANRLTTFRTVILPELFPALLMGAGMMFARATGEYGSVIFIAGNIPM
QSEILPLIIISKLEQFDIQGASAVALFMLMISFVILLAINLLQWRLSRRVGAK

Sequences:

>Translated_293_residues
MNQSAPSTAPIRKRSWLTRLRQRNVLPGFGLSMGITVLSLSLLVILPFAMMAYTTTQMGLSGFIEAISQLQVKAAIILSL
KMSFYAMLTNMVFGTLVAWVLVRYDFIGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQFFDKVGIQVAFTPIGIW
LALVVVSFPFIVRAVQPVLEELSVEYEEAAAVLGANRLTTFRTVILPELFPALLMGAGMMFARATGEYGSVIFIAGNIPM
QSEILPLIIISKLEQFDIQGASAVALFMLMISFVILLAINLLQWRLSRRVGAK
>Mature_293_residues
MNQSAPSTAPIRKRSWLTRLRQRNVLPGFGLSMGITVLSLSLLVILPFAMMAYTTTQMGLSGFIEAISQLQVKAAIILSL
KMSFYAMLTNMVFGTLVAWVLVRYDFIGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQFFDKVGIQVAFTPIGIW
LALVVVSFPFIVRAVQPVLEELSVEYEEAAAVLGANRLTTFRTVILPELFPALLMGAGMMFARATGEYGSVIFIAGNIPM
QSEILPLIIISKLEQFDIQGASAVALFMLMISFVILLAINLLQWRLSRRVGAK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=48.3271375464684, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI87082099, Length=273, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786980, Length=224, Percent_Identity=37.0535714285714, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787714, Length=263, Percent_Identity=31.5589353612167, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31968; Mature: 31968

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]