Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 148652730

GI number: 148652730

Start: 1103202

End: 1104110

Strand: Direct

Name: rnhB

Synonym: PsycPRwf_0923

Alternate gene names: 148652730

Gene position: 1103202-1104110 (Clockwise)

Preceding gene: 148652729

Following gene: 148652735

Centisome position: 37.03

GC content: 48.51

Gene sequence:

>909_bases
ATGGTAATTGAACAAAGTATTTATTCTTCCTCGCAAAGCACAGGTACGGCCCAAGCCAACTTAGAGCTGCCTTTATCATC
ACCGCTTCATTTAAGCGGGCGGATAAATACCAAGGCGTTATTTAGCCCAGACAATCAGAGTCAATCTGGTGGGTTTGAGT
TTGAAATAGGGGTAGATGAGGTCGGTCGTGGCCCTTTATACGGTAGTGTGGTAGTGGCAGCAGCGATATTGCCAAAAGCT
TGGTCTGGGGAGACGGAGGTAGGCTTACTACAAGACACACCACTTGCGATATTGACCGATTCCAAAAAACTGACTGAGCG
TAAGCGAGAAAAGCTATTTGAACCGGTAAAGCAGCATGCTTTGGCTTATCTGGTCGTAGAAGTGCCTGCTGGCGTCATTG
ATGAGATTAATATCCTGCAAGCCACTATACTAGGGATGCGCGTAGCTTGTGAGCAGCTGATGGTTGAGATTACCAAAGTG
TGGCGTGATGCATCTGAATTGCCGACTGCACCCTCAACACCACTCGGGTTTAAATTGCTGATTGATGGCAATAAAGTACC
TGATTTGGATGAGGCGCGATTGGCAACTCATGGTATTTGCTTAGCAGATATGACGGTCACACAGCCAGCAGGTATGGTCA
AATTCTGCGCTGAAGCTTGGGTCAAAGGCGATGCACGACATAATGCCATTGCCGCGGCCAGTGTCTTAGCCAAAGTGTAC
CGAGACAGACAGCTCATTGCAGATGGGGCTCGATATCCAGGCTATGGCTTAGAGGGGCATAAAGGCTATCCTACGAAAGC
GCATGTTGAAGCCATTGCTCGCTTAGGTGTATTGCCTGAGCACAGACGTAGCTTTAAGCCGGTACAACAGGCTATTGAGG
GCACATTAGCGGGCACACACTATAGCTGA

Upstream 100 bases:

>100_bases
GTCATCAATACCTGGCATGATTATCAAAATCAGCGGTTAAATGGTCAGCCATTATTGTAGCGGACAAAAAATTAATTCAT
AAAAAGATGTTGTGGTTATG

Downstream 100 bases:

>100_bases
TACGCTTAAGGTAGAGCAGGTTTCGGTGCGCCGCTGGTAACAATGAGTCAGCTACAGTTGTGGCTAATTTTGCTGTCGCT
GCTGCGGTGGATGCTGGCGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MVIEQSIYSSSQSTGTAQANLELPLSSPLHLSGRINTKALFSPDNQSQSGGFEFEIGVDEVGRGPLYGSVVVAAAILPKA
WSGETEVGLLQDTPLAILTDSKKLTERKREKLFEPVKQHALAYLVVEVPAGVIDEINILQATILGMRVACEQLMVEITKV
WRDASELPTAPSTPLGFKLLIDGNKVPDLDEARLATHGICLADMTVTQPAGMVKFCAEAWVKGDARHNAIAAASVLAKVY
RDRQLIADGARYPGYGLEGHKGYPTKAHVEAIARLGVLPEHRRSFKPVQQAIEGTLAGTHYS

Sequences:

>Translated_302_residues
MVIEQSIYSSSQSTGTAQANLELPLSSPLHLSGRINTKALFSPDNQSQSGGFEFEIGVDEVGRGPLYGSVVVAAAILPKA
WSGETEVGLLQDTPLAILTDSKKLTERKREKLFEPVKQHALAYLVVEVPAGVIDEINILQATILGMRVACEQLMVEITKV
WRDASELPTAPSTPLGFKLLIDGNKVPDLDEARLATHGICLADMTVTQPAGMVKFCAEAWVKGDARHNAIAAASVLAKVY
RDRQLIADGARYPGYGLEGHKGYPTKAHVEAIARLGVLPEHRRSFKPVQQAIEGTLAGTHYS
>Mature_302_residues
MVIEQSIYSSSQSTGTAQANLELPLSSPLHLSGRINTKALFSPDNQSQSGGFEFEIGVDEVGRGPLYGSVVVAAAILPKA
WSGETEVGLLQDTPLAILTDSKKLTERKREKLFEPVKQHALAYLVVEVPAGVIDEINILQATILGMRVACEQLMVEITKV
WRDASELPTAPSTPLGFKLLIDGNKVPDLDEARLATHGICLADMTVTQPAGMVKFCAEAWVKGDARHNAIAAASVLAKVY
RDRQLIADGARYPGYGLEGHKGYPTKAHVEAIARLGVLPEHRRSFKPVQQAIEGTLAGTHYS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=236, Percent_Identity=39.4067796610169, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PSYWF (A5WDY2)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279823.1
- ProteinModelPortal:   A5WDY2
- SMR:   A5WDY2
- STRING:   A5WDY2
- GeneID:   5205088
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0923
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RMELRIT
- ProtClustDB:   CLSK839110
- BioCyc:   PSP56811:PSYCPRWF_0923-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 32562; Mature: 32562

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIEQSIYSSSQSTGTAQANLELPLSSPLHLSGRINTKALFSPDNQSQSGGFEFEIGVDE
CCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCEEEECCCCCCCCCCEEEEECHHH
VGRGPLYGSVVVAAAILPKAWSGETEVGLLQDTPLAILTDSKKLTERKREKLFEPVKQHA
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHH
LAYLVVEVPAGVIDEINILQATILGMRVACEQLMVEITKVWRDASELPTAPSTPLGFKLL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEE
IDGNKVPDLDEARLATHGICLADMTVTQPAGMVKFCAEAWVKGDARHNAIAAASVLAKVY
ECCCCCCCCCHHHHHHCCEEEECCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RDRQLIADGARYPGYGLEGHKGYPTKAHVEAIARLGVLPEHRRSFKPVQQAIEGTLAGTH
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
YS
CC
>Mature Secondary Structure
MVIEQSIYSSSQSTGTAQANLELPLSSPLHLSGRINTKALFSPDNQSQSGGFEFEIGVDE
CCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCEEEECCCCCCCCCCEEEEECHHH
VGRGPLYGSVVVAAAILPKAWSGETEVGLLQDTPLAILTDSKKLTERKREKLFEPVKQHA
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHH
LAYLVVEVPAGVIDEINILQATILGMRVACEQLMVEITKVWRDASELPTAPSTPLGFKLL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEE
IDGNKVPDLDEARLATHGICLADMTVTQPAGMVKFCAEAWVKGDARHNAIAAASVLAKVY
ECCCCCCCCCHHHHHHCCEEEECCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RDRQLIADGARYPGYGLEGHKGYPTKAHVEAIARLGVLPEHRRSFKPVQQAIEGTLAGTH
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
YS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA