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The map label for this gene is queF [H]

Identifier: 148652420

GI number: 148652420

Start: 730774

End: 731676

Strand: Direct

Name: queF [H]

Synonym: PsycPRwf_0608

Alternate gene names: 148652420

Gene position: 730774-731676 (Clockwise)

Preceding gene: 148652419

Following gene: 148652421

Centisome position: 24.53

GC content: 47.4

Gene sequence:

>903_bases
ATGAGCATTCATGGCGTCTTAGGCGAAACCACCAGCTATCCAAAAACCTATCAGCCCGATACCCTTTATGCCATTGCACG
CTCTTTAGGCCGTGATGCCATTGGTTGGGATGATGATAAATTGGCGGTGGGTGTAGATTGGTGGCATGCTTTTGAAATTT
CATGGCTAAATCCTCAAGGCATCTCGCAGATGGCGGTAGCGCGCTTTTCTGTCCCCGCTCATTCGCCAAACATTGTTGAA
TCCAAGTCTTTAAAGCTGTATTTAAACAGCTTAAACTTCACCGAGTTTGACAGTAAGGCTGCGGTCGAGCAAACCATTGC
TAAAGATTTATCAGCCTGTGTGGGTGCTGATGTGGGCGTTGAGCTGTTTGATTTAGATGCCATTAGTGCTGGCGATACAC
ACCATTCAGGATTTTTGATTGCGCATCCGCAAGGCAAATGCATCGATAACGCATTGCAAGGCGCTGCTGTAAAAATTGAG
CCGGTTGAACACCCTGATGCCCGCTTATTGTCCGCACATGACACGCCAGTCGATAACAATTTAGCCAGCGCTAAGTCCCC
ATTAAGCTCGACGGCACAAGACTATCAGCTGTACTCAAATCTGCTGCGTAGTAATTGCCCGGTGACCAATCAGCCAGACT
GGGGCACGTTATCAATTGAAATGACCACTGATATGGCTGTCGATGAAGCCAGCTTATTGACCTATATTTTAAGCTTCCGT
CAGCATAATGGCTTTCATGAGCAGTGTGTCGAGCAGATTTTTAGCGATGTGAGCCGCTACTTTAAGCCCTCAAAACTTAT
GGTACGGGCTTGGTATACTCGTCGCGGCGGTATTGATATTAATCCTTGCCGAGTTAGTGACCCAAGCTTATTACCAGCGC
CTAGCCGTTTGGTACGTCAGTAA

Upstream 100 bases:

>100_bases
ATGCCGTATTTGCTTTGGCGTAACCTGTATTAACCCAAGCTTAAACCGCTCAAACCTCTTAAGATCTTGAGCAAACACAT
CAATTAGTAAGGATAAGATT

Downstream 100 bases:

>100_bases
TCAGCGTCTCTTTTGAGGATAAAAATAGTGCTATATTAAAAAAAATATTAAGCCAAAATTAAGGGGGAGTAGTGGGGTAA
TATAACCAATACTTTCCCTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSIHGVLGETTSYPKTYQPDTLYAIARSLGRDAIGWDDDKLAVGVDWWHAFEISWLNPQGISQMAVARFSVPAHSPNIVE
SKSLKLYLNSLNFTEFDSKAAVEQTIAKDLSACVGADVGVELFDLDAISAGDTHHSGFLIAHPQGKCIDNALQGAAVKIE
PVEHPDARLLSAHDTPVDNNLASAKSPLSSTAQDYQLYSNLLRSNCPVTNQPDWGTLSIEMTTDMAVDEASLLTYILSFR
QHNGFHEQCVEQIFSDVSRYFKPSKLMVRAWYTRRGGIDINPCRVSDPSLLPAPSRLVRQ

Sequences:

>Translated_300_residues
MSIHGVLGETTSYPKTYQPDTLYAIARSLGRDAIGWDDDKLAVGVDWWHAFEISWLNPQGISQMAVARFSVPAHSPNIVE
SKSLKLYLNSLNFTEFDSKAAVEQTIAKDLSACVGADVGVELFDLDAISAGDTHHSGFLIAHPQGKCIDNALQGAAVKIE
PVEHPDARLLSAHDTPVDNNLASAKSPLSSTAQDYQLYSNLLRSNCPVTNQPDWGTLSIEMTTDMAVDEASLLTYILSFR
QHNGFHEQCVEQIFSDVSRYFKPSKLMVRAWYTRRGGIDINPCRVSDPSLLPAPSRLVRQ
>Mature_299_residues
SIHGVLGETTSYPKTYQPDTLYAIARSLGRDAIGWDDDKLAVGVDWWHAFEISWLNPQGISQMAVARFSVPAHSPNIVES
KSLKLYLNSLNFTEFDSKAAVEQTIAKDLSACVGADVGVELFDLDAISAGDTHHSGFLIAHPQGKCIDNALQGAAVKIEP
VEHPDARLLSAHDTPVDNNLASAKSPLSSTAQDYQLYSNLLRSNCPVTNQPDWGTLSIEMTTDMAVDEASLLTYILSFRQ
HNGFHEQCVEQIFSDVSRYFKPSKLMVRAWYTRRGGIDINPCRVSDPSLLPAPSRLVRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=296, Percent_Identity=41.554054054054, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 33048; Mature: 32917

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHGVLGETTSYPKTYQPDTLYAIARSLGRDAIGWDDDKLAVGVDWWHAFEISWLNPQG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEEECCCC
ISQMAVARFSVPAHSPNIVESKSLKLYLNSLNFTEFDSKAAVEQTIAKDLSACVGADVGV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ELFDLDAISAGDTHHSGFLIAHPQGKCIDNALQGAAVKIEPVEHPDARLLSAHDTPVDNN
EEEEECCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCC
LASAKSPLSSTAQDYQLYSNLLRSNCPVTNQPDWGTLSIEMTTDMAVDEASLLTYILSFR
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
QHNGFHEQCVEQIFSDVSRYFKPSKLMVRAWYTRRGGIDINPCRVSDPSLLPAPSRLVRQ
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SIHGVLGETTSYPKTYQPDTLYAIARSLGRDAIGWDDDKLAVGVDWWHAFEISWLNPQG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEEECCCC
ISQMAVARFSVPAHSPNIVESKSLKLYLNSLNFTEFDSKAAVEQTIAKDLSACVGADVGV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ELFDLDAISAGDTHHSGFLIAHPQGKCIDNALQGAAVKIEPVEHPDARLLSAHDTPVDNN
EEEEECCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCC
LASAKSPLSSTAQDYQLYSNLLRSNCPVTNQPDWGTLSIEMTTDMAVDEASLLTYILSFR
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
QHNGFHEQCVEQIFSDVSRYFKPSKLMVRAWYTRRGGIDINPCRVSDPSLLPAPSRLVRQ
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA