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The map label for this gene is rlmE

Identifier: 148652346

GI number: 148652346

Start: 637759

End: 638391

Strand: Direct

Name: rlmE

Synonym: PsycPRwf_0534

Alternate gene names: 148652346

Gene position: 637759-638391 (Clockwise)

Preceding gene: 148652344

Following gene: 148652347

Centisome position: 21.41

GC content: 48.34

Gene sequence:

>633_bases
TTGGCGACACGTATTACCAATAAAGGCCTATCAAAAAGCAGTAAGGCTTGGATGAAAGAGCATTTGGATGACCATTATGT
GCAATTGGCGCAAAAAGAGGGTTACCGCGCTCGCGCTGCCTATAAACTATTGGAAATCAACGAAAAAACCAATCTCATTA
AAAAAGGGATGACGGTGGTTGATTTGGGCAGTGCCCCTGGCAGCTGGTCACAGGTGGCAGGCAGACTGGTAGGGGATGAG
GGCATACTCATCGCTTCAGACATCTTACCGATGGATGCCTTAGAGAATGTCACCTTTATTCAAGGTGACTTCAGAGAGGA
GGCGGTGTTTGATCGCTTGATGAGTGAAGTGGGTGACAGACAGGTAGATGTGGTGTTATCTGATATGGCACCCAATACGT
CAGGGCATACCGCCGTAGATCAGCCACGTATGATTTATCTGTGTGAGCTGGCAGTAGACTTTGCTTTGCGCGTACTGCCA
AAAGGTGGGTCGTTGGTAATGAAGGTATTCCAAGGCGAGGGTGAGCAGCAATTACGCCAGCAGCTACAGTCACAGTTTTC
AAAAGTTCGCACCATTAAGCCTGCCGCTTCTCGTCCGCGCTCAAAAGAGGTGTTTTGGGTAGCTACCAAATAA

Upstream 100 bases:

>100_bases
GCTAGCCATCCGCCCAGTTAGCCCCTTTATTGGGGCAAATAATGCTAGAATTAAGATTAAAATTTAGTTATGAATTGTCT
GAAAATATAGTGAGGATATT

Downstream 100 bases:

>100_bases
AGCGGTATGGCGCTGAGCGTTGCTTATGATGCGCATAATCCCATTCACACCGACAACAGAGAATCAACACAGCAGCTAAT
ATTTAGACTTTATTGATGCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MATRITNKGLSKSSKAWMKEHLDDHYVQLAQKEGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGRLVGDE
GILIASDILPMDALENVTFIQGDFREEAVFDRLMSEVGDRQVDVVLSDMAPNTSGHTAVDQPRMIYLCELAVDFALRVLP
KGGSLVMKVFQGEGEQQLRQQLQSQFSKVRTIKPAASRPRSKEVFWVATK

Sequences:

>Translated_210_residues
MATRITNKGLSKSSKAWMKEHLDDHYVQLAQKEGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGRLVGDE
GILIASDILPMDALENVTFIQGDFREEAVFDRLMSEVGDRQVDVVLSDMAPNTSGHTAVDQPRMIYLCELAVDFALRVLP
KGGSLVMKVFQGEGEQQLRQQLQSQFSKVRTIKPAASRPRSKEVFWVATK
>Mature_209_residues
ATRITNKGLSKSSKAWMKEHLDDHYVQLAQKEGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGRLVGDEG
ILIASDILPMDALENVTFIQGDFREEAVFDRLMSEVGDRQVDVVLSDMAPNTSGHTAVDQPRMIYLCELAVDFALRVLPK
GGSLVMKVFQGEGEQQLRQQLQSQFSKVRTIKPAASRPRSKEVFWVATK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=219, Percent_Identity=35.6164383561644, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI194097365, Length=185, Percent_Identity=35.1351351351351, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI29029591, Length=187, Percent_Identity=36.8983957219251, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI29029589, Length=187, Percent_Identity=36.8983957219251, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI7110661, Length=187, Percent_Identity=36.8983957219251, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=53.6585365853659, Blast_Score=218, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17553474, Length=196, Percent_Identity=34.6938775510204, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=34.0540540540541, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71987561, Length=185, Percent_Identity=34.0540540540541, Blast_Score=113, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17553860, Length=185, Percent_Identity=34.0540540540541, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17554650, Length=185, Percent_Identity=31.8918918918919, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=34.8717948717949, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=200, Percent_Identity=36, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321302, Length=289, Percent_Identity=26.9896193771626, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356387, Length=210, Percent_Identity=38.0952380952381, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24647580, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18859957, Length=185, Percent_Identity=34.0540540540541, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=34.1968911917098, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_PSYWF (A5WCU8)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279439.1
- ProteinModelPortal:   A5WCU8
- SMR:   A5WCU8
- STRING:   A5WCU8
- GeneID:   5206838
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0534
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK839442
- BioCyc:   PSP56811:PSYCPRWF_0534-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23384; Mature: 23253

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: ACT_SITE 168-168 BINDING 67-67 BINDING 69-69 BINDING 87-87 BINDING 103-103 BINDING 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRITNKGLSKSSKAWMKEHLDDHYVQLAQKEGYRARAAYKLLEINEKTNLIKKGMTVV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCEEE
DLGSAPGSWSQVAGRLVGDEGILIASDILPMDALENVTFIQGDFREEAVFDRLMSEVGDR
EECCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHCCC
QVDVVLSDMAPNTSGHTAVDQPRMIYLCELAVDFALRVLPKGGSLVMKVFQGEGEQQLRQ
EEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHH
QLQSQFSKVRTIKPAASRPRSKEVFWVATK
HHHHHHHHHHCCCCHHCCCCCCCEEEEECC
>Mature Secondary Structure 
ATRITNKGLSKSSKAWMKEHLDDHYVQLAQKEGYRARAAYKLLEINEKTNLIKKGMTVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCEEE
DLGSAPGSWSQVAGRLVGDEGILIASDILPMDALENVTFIQGDFREEAVFDRLMSEVGDR
EECCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHCCC
QVDVVLSDMAPNTSGHTAVDQPRMIYLCELAVDFALRVLPKGGSLVMKVFQGEGEQQLRQ
EEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHH
QLQSQFSKVRTIKPAASRPRSKEVFWVATK
HHHHHHHHHHCCCCHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA