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The map label for this gene is helD [H]

Identifier: 148643831

GI number: 148643831

Start: 1830800

End: 1832632

Strand: Reverse

Name: helD [H]

Synonym: Msm_1771

Alternate gene names: 148643831

Gene position: 1832632-1830800 (Counterclockwise)

Preceding gene: 148643837

Following gene: 148643825

Centisome position: 98.89

GC content: 31.21

Gene sequence:

>1833_bases
ATGGATTTTTGTAGTGAATGCGGATCAATGATACTAAATGGCAGCAAATGCCCGATGTGCGGCTGTATAAAAACTGCTGA
AACTAAGGAAGTTAAAAAAACACCACGGCCAGAAAAGAAAAAAACAAATAAAAAGCCAGTTATTGAAACATTCACTAAAG
ATATTAAAACAGAACCTGAAAAAATAGCCAGCAAAAACGGCAAGTACTTGAAAAAAGACTACAAAAGTATTGATGATTTG
GATGATAAAACTAGACAGGAGCTGCTGGCTAATGATAAGTTTTTTAAAACAGTAAACAGCAAACCTTTAGACACACAGCA
AAAAATAGCCTGTGTTTTAGATTCCAAAAATGTTCAAATCATTGCAGGAGCAGGAACAGGAAAAACATTTACTTTAATTG
CAAAAATAAAATATCTGATAGCTAAAAAGCATGTCAGACCTGAAGATATTTTGTGTCTGTCATTTTCAAATACTTCTGTC
AGGGATTTAAAAGGAAAGCTTCCGGAAAATGTTGAGATAAGGACATTTCACAGTCTGGGAAGATCAGTCATTAAGCAGCA
CCATAAAGTGTCAGTTATTGAAAACAATGAAATATTGGCTATAATAAATGATTATCTGGCCAATGCAAATACTGATACAT
TAAAAGATATTCTTGAATTTTGCGATTCATATTTTTTAAACATTGAAAGCTACATCATTAGAAGAAATGACAGAAAAGAG
TTATTGAATGAATTGAAAGAAGCATTTACAAAAGTGGCATTTAAACCCCTGCTTGCAGATTTTATAAATTTATTTAAGGG
CAACAATTTTACAAAAGATTATTTCAGCTATTTCAGAAGCAGTAACGATGACAAGGACCATGACATCTTCCTAAAAATCG
CTGAAGATTTCTACAGTTACTATCAGAAATACTTGGATATGCACCAGCAGATTGATTTTAATGACATGATAAATGAATCA
AGCAAGCTGATTAAAAAATACGGGCTGAAAAAACATTACAGATACATACTGGTTGATGAATATCAGGATACCACATATAC
CAATTATAATCTGATTAAAAGCCTGCAGGATAAAACAGATGCACATCTGACAGTTGTAGGTGATGACTGGCAGTCAATAT
ACGGATTTAGAGGAACAAACATTGAATTTTTCTCACATTTTGAAGAGTATTTTGAAAATCCTCAAAGAATATTCATTGAA
AAAACATACAGGAACCCTCAGGAGCTGATTGACATAGCAGGTAGCTTCATTATGAAAAACCCTAAACAAATTAGGAAATC
TCTTAAGTCTCCTAAAAATCTGAAAAAACCTGTTAAAATAATCTATCCTCAAAAAAATCCTATAGAAAAAAGAGAAATGA
TTTATAATCTTATAAAAGATCTCTCTAAAAAATCCGAAGATGTATTAATCTTAGGCAGAACAAACAACAACATCAACCAG
TTTATAAAACACAGAAATCTGGTTAAAAAAGGCAATCTGAAAAAAGACAAATTCTTACGAATTTTAGACAAAACAGACAA
ATCAATGAATAATGTTTACTACAGAACCCTGCATTCATCTAAAGGTCTGGAAGCAGATAATGTGATTATAATCAATATGG
TTGATGACTATTTAGGTTTTCCATCAAAATTAAAAACCCACAGTGTTTTGAATTATGTAAATACCAAAAATTATGATTAC
AAATATTCAGAAGAACGCAGATTATTCTATGTAGGTTTAACCCGCTCTAAAAATAATGTTTACTTAATAAGTGATAAAAA
TAACCCTTCAGAATTCATTGAAGAGCTGATAGATGACAGCTTGGAAAATTTAGAAATAATTGAGTATAACTGA

Upstream 100 bases:

>100_bases
ATTTTTAATTAAACTAGTATTTAAAATATGTATAAAACAGAAATACTTTTCAGACACTCTTAGGAAAGCTATTTTTAAAT
GTTAATAAAAAATATATATT

Downstream 100 bases:

>100_bases
TTCTGTTTTAAAAACAACGAAAGGTATTGAAAAGCCACATAACAAACCTGTAACAAATCTTAAAGTGTTATTGCTTGTTC
TTAAACCGTAATACTGTGTA

Product: UvrD/REP family DNA helicase

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MDFCSECGSMILNGSKCPMCGCIKTAETKEVKKTPRPEKKKTNKKPVIETFTKDIKTEPEKIASKNGKYLKKDYKSIDDL
DDKTRQELLANDKFFKTVNSKPLDTQQKIACVLDSKNVQIIAGAGTGKTFTLIAKIKYLIAKKHVRPEDILCLSFSNTSV
RDLKGKLPENVEIRTFHSLGRSVIKQHHKVSVIENNEILAIINDYLANANTDTLKDILEFCDSYFLNIESYIIRRNDRKE
LLNELKEAFTKVAFKPLLADFINLFKGNNFTKDYFSYFRSSNDDKDHDIFLKIAEDFYSYYQKYLDMHQQIDFNDMINES
SKLIKKYGLKKHYRYILVDEYQDTTYTNYNLIKSLQDKTDAHLTVVGDDWQSIYGFRGTNIEFFSHFEEYFENPQRIFIE
KTYRNPQELIDIAGSFIMKNPKQIRKSLKSPKNLKKPVKIIYPQKNPIEKREMIYNLIKDLSKKSEDVLILGRTNNNINQ
FIKHRNLVKKGNLKKDKFLRILDKTDKSMNNVYYRTLHSSKGLEADNVIIINMVDDYLGFPSKLKTHSVLNYVNTKNYDY
KYSEERRLFYVGLTRSKNNVYLISDKNNPSEFIEELIDDSLENLEIIEYN

Sequences:

>Translated_610_residues
MDFCSECGSMILNGSKCPMCGCIKTAETKEVKKTPRPEKKKTNKKPVIETFTKDIKTEPEKIASKNGKYLKKDYKSIDDL
DDKTRQELLANDKFFKTVNSKPLDTQQKIACVLDSKNVQIIAGAGTGKTFTLIAKIKYLIAKKHVRPEDILCLSFSNTSV
RDLKGKLPENVEIRTFHSLGRSVIKQHHKVSVIENNEILAIINDYLANANTDTLKDILEFCDSYFLNIESYIIRRNDRKE
LLNELKEAFTKVAFKPLLADFINLFKGNNFTKDYFSYFRSSNDDKDHDIFLKIAEDFYSYYQKYLDMHQQIDFNDMINES
SKLIKKYGLKKHYRYILVDEYQDTTYTNYNLIKSLQDKTDAHLTVVGDDWQSIYGFRGTNIEFFSHFEEYFENPQRIFIE
KTYRNPQELIDIAGSFIMKNPKQIRKSLKSPKNLKKPVKIIYPQKNPIEKREMIYNLIKDLSKKSEDVLILGRTNNNINQ
FIKHRNLVKKGNLKKDKFLRILDKTDKSMNNVYYRTLHSSKGLEADNVIIINMVDDYLGFPSKLKTHSVLNYVNTKNYDY
KYSEERRLFYVGLTRSKNNVYLISDKNNPSEFIEELIDDSLENLEIIEYN
>Mature_610_residues
MDFCSECGSMILNGSKCPMCGCIKTAETKEVKKTPRPEKKKTNKKPVIETFTKDIKTEPEKIASKNGKYLKKDYKSIDDL
DDKTRQELLANDKFFKTVNSKPLDTQQKIACVLDSKNVQIIAGAGTGKTFTLIAKIKYLIAKKHVRPEDILCLSFSNTSV
RDLKGKLPENVEIRTFHSLGRSVIKQHHKVSVIENNEILAIINDYLANANTDTLKDILEFCDSYFLNIESYIIRRNDRKE
LLNELKEAFTKVAFKPLLADFINLFKGNNFTKDYFSYFRSSNDDKDHDIFLKIAEDFYSYYQKYLDMHQQIDFNDMINES
SKLIKKYGLKKHYRYILVDEYQDTTYTNYNLIKSLQDKTDAHLTVVGDDWQSIYGFRGTNIEFFSHFEEYFENPQRIFIE
KTYRNPQELIDIAGSFIMKNPKQIRKSLKSPKNLKKPVKIIYPQKNPIEKREMIYNLIKDLSKKSEDVLILGRTNNNINQ
FIKHRNLVKKGNLKKDKFLRILDKTDKSMNNVYYRTLHSSKGLEADNVIIINMVDDYLGFPSKLKTHSVLNYVNTKNYDY
KYSEERRLFYVGLTRSKNNVYLISDKNNPSEFIEELIDDSLENLEIIEYN

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=526, Percent_Identity=25.6653992395437, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI2367296, Length=396, Percent_Identity=24.7474747474747, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI48994965, Length=355, Percent_Identity=26.1971830985915, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=396, Percent_Identity=27.5252525252525, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 71446; Mature: 71446

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS01030 RNA_POL_M_15KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFCSECGSMILNGSKCPMCGCIKTAETKEVKKTPRPEKKKTNKKPVIETFTKDIKTEPE
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHCCCCCCHHHHHHHHHCCCHH
KIASKNGKYLKKDYKSIDDLDDKTRQELLANDKFFKTVNSKPLDTQQKIACVLDSKNVQI
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHEEEEEECCCEEE
IAGAGTGKTFTLIAKIKYLIAKKHVRPEDILCLSFSNTSVRDLKGKLPENVEIRTFHSLG
EEECCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCEEHHHHHHH
RSVIKQHHKVSVIENNEILAIINDYLANANTDTLKDILEFCDSYFLNIESYIIRRNDRKE
HHHHHHCCCEEEEECCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHH
LLNELKEAFTKVAFKPLLADFINLFKGNNFTKDYFSYFRSSNDDKDHDIFLKIAEDFYSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YQKYLDMHQQIDFNDMINESSKLIKKYGLKKHYRYILVDEYQDTTYTNYNLIKSLQDKTD
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCCCHHHHHHHHCCCC
AHLTVVGDDWQSIYGFRGTNIEFFSHFEEYFENPQRIFIEKTYRNPQELIDIAGSFIMKN
CEEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCC
PKQIRKSLKSPKNLKKPVKIIYPQKNPIEKREMIYNLIKDLSKKSEDVLILGRTNNNINQ
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
FIKHRNLVKKGNLKKDKFLRILDKTDKSMNNVYYRTLHSSKGLEADNVIIINMVDDYLGF
HHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHEEEECCCCCCCCCCEEEEEECHHHCCC
PSKLKTHSVLNYVNTKNYDYKYSEERRLFYVGLTRSKNNVYLISDKNNPSEFIEELIDDS
CHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCC
LENLEIIEYN
HHCCEEEEEC
>Mature Secondary Structure
MDFCSECGSMILNGSKCPMCGCIKTAETKEVKKTPRPEKKKTNKKPVIETFTKDIKTEPE
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHCCCCCCHHHHHHHHHCCCHH
KIASKNGKYLKKDYKSIDDLDDKTRQELLANDKFFKTVNSKPLDTQQKIACVLDSKNVQI
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHEEEEEECCCEEE
IAGAGTGKTFTLIAKIKYLIAKKHVRPEDILCLSFSNTSVRDLKGKLPENVEIRTFHSLG
EEECCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCEEHHHHHHH
RSVIKQHHKVSVIENNEILAIINDYLANANTDTLKDILEFCDSYFLNIESYIIRRNDRKE
HHHHHHCCCEEEEECCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHH
LLNELKEAFTKVAFKPLLADFINLFKGNNFTKDYFSYFRSSNDDKDHDIFLKIAEDFYSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YQKYLDMHQQIDFNDMINESSKLIKKYGLKKHYRYILVDEYQDTTYTNYNLIKSLQDKTD
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCCCHHHHHHHHCCCC
AHLTVVGDDWQSIYGFRGTNIEFFSHFEEYFENPQRIFIEKTYRNPQELIDIAGSFIMKN
CEEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCC
PKQIRKSLKSPKNLKKPVKIIYPQKNPIEKREMIYNLIKDLSKKSEDVLILGRTNNNINQ
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
FIKHRNLVKKGNLKKDKFLRILDKTDKSMNNVYYRTLHSSKGLEADNVIIINMVDDYLGF
HHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHEEEECCCCCCCCCCEEEEEECHHHCCC
PSKLKTHSVLNYVNTKNYDYKYSEERRLFYVGLTRSKNNVYLISDKNNPSEFIEELIDDS
CHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCC
LENLEIIEYN
HHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]