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The map label for this gene is fdhF [C]

Identifier: 148643523

GI number: 148643523

Start: 1487416

End: 1488525

Strand: Reverse

Name: fdhF [C]

Synonym: Msm_1463

Alternate gene names: 148643523

Gene position: 1488525-1487416 (Counterclockwise)

Preceding gene: 148643524

Following gene: 148643522

Centisome position: 80.32

GC content: 26.31

Gene sequence:

>1110_bases
ATGTTAAATTTAAAGCATACAATATGCCCATCATGTAGTACTGGCTGTGGCATAACTTTAATCTCAAATAATAAAGAATT
ACTTGGAACTTATCCCTACAAAAGACATGAAATTAATGAAGGAAAAAACTGTAAAAATGGCAGATACTGTTTTACAAAAT
ATTCATTTAATAAAATCGATGAACCTTCCATAGTAAATAACGGAAAATTAAATGAATCTGATTTTAAAAGCATTATAGAA
CAGTTTATAGAAGATATAAAAAATGTAGAACCGGAACAGTTCGGGATTTTATGTTCAGGAAATTCAACAAATGAAGAATT
GGATTTGATAAAAAAATTCAGTGAAAACTATGGAAACAAATTATTCTTATATGAAAATGGATTTGTAAACCTTGACAAAA
CAGCTACAAGTTACGATGACATCAACAATTCCAAAACCATTTTGATTATCGGAGACTTATATGATGAAAACCCTTTAGTT
GCCAGAAGAGTAATAATGGCTAAAGAAAATGGTGCAAAAGTTATTTGTGCAGATAATGAAAATAAAAATACCACTTCCAT
AAACTCCGATAAATATATTCAATTTGACTCTGTAAGTGAATTCCTCGATAGTTTAGATGAAGAAATTTTAGATGAATTAA
ACGAAGATGCAATTATAATATTCAATAAGGTAGACAATGAAGAAAACTGCCAAAAAATATCTGAAATAGGATTAAAAACC
AATGCTAAAATTTTACCTATATACAAAAAATGTAACAGTAAAGGAGCATTAAATAAAATAGATCCTATTAATTTAAATGA
ATTAAAAGAAGAATTGAAAAATATTGAAGTTTTATTAATTGTCAATGAAAATCCTCTCGATTATATAACTGAAGACGATT
TAAACAGCATAAAAAAGATAGTTACCATTTCTTATTTTAAAAATGATATTGTAAATAAAAGCGATTATGTAATTCCTGGA
AAACCTTGGGCTTTTAAAGAAGGATCTTACACAAACAGTGTAAATACAAATCAAAACTTTAAAATAGCTGTTCCAGACAT
TGTAAATGAAAATCTTTCTGAAATTGAAATTTTAATGAAAATTTCCGAAAAATTAGATATTGATTTATAG

Upstream 100 bases:

>100_bases
ACAAGACAGAATGTTTATGAAATAAAAAACGAAAACGAAACAACATTTTCAAAAGCATTTTTTGAAAATCAATTGATAAA
TTGATAGAGTAGTGATATAT

Downstream 100 bases:

>100_bases
AAGGTATGTTTATGAGTTCAAATTATATTCTAGCTCGTTCTAAAGACGAAGAGATATTGAAAAAAGCTGCTGTTGGAGGA
GCTGTAACCGGAATTTTACA

Product: formate dehydrogenase, alpha subunit, FdhA

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLNLKHTICPSCSTGCGITLISNNKELLGTYPYKRHEINEGKNCKNGRYCFTKYSFNKIDEPSIVNNGKLNESDFKSIIE
QFIEDIKNVEPEQFGILCSGNSTNEELDLIKKFSENYGNKLFLYENGFVNLDKTATSYDDINNSKTILIIGDLYDENPLV
ARRVIMAKENGAKVICADNENKNTTSINSDKYIQFDSVSEFLDSLDEEILDELNEDAIIIFNKVDNEENCQKISEIGLKT
NAKILPIYKKCNSKGALNKIDPINLNELKEELKNIEVLLIVNENPLDYITEDDLNSIKKIVTISYFKNDIVNKSDYVIPG
KPWAFKEGSYTNSVNTNQNFKIAVPDIVNENLSEIEILMKISEKLDIDL

Sequences:

>Translated_369_residues
MLNLKHTICPSCSTGCGITLISNNKELLGTYPYKRHEINEGKNCKNGRYCFTKYSFNKIDEPSIVNNGKLNESDFKSIIE
QFIEDIKNVEPEQFGILCSGNSTNEELDLIKKFSENYGNKLFLYENGFVNLDKTATSYDDINNSKTILIIGDLYDENPLV
ARRVIMAKENGAKVICADNENKNTTSINSDKYIQFDSVSEFLDSLDEEILDELNEDAIIIFNKVDNEENCQKISEIGLKT
NAKILPIYKKCNSKGALNKIDPINLNELKEELKNIEVLLIVNENPLDYITEDDLNSIKKIVTISYFKNDIVNKSDYVIPG
KPWAFKEGSYTNSVNTNQNFKIAVPDIVNENLSEIEILMKISEKLDIDL
>Mature_369_residues
MLNLKHTICPSCSTGCGITLISNNKELLGTYPYKRHEINEGKNCKNGRYCFTKYSFNKIDEPSIVNNGKLNESDFKSIIE
QFIEDIKNVEPEQFGILCSGNSTNEELDLIKKFSENYGNKLFLYENGFVNLDKTATSYDDINNSKTILIIGDLYDENPLV
ARRVIMAKENGAKVICADNENKNTTSINSDKYIQFDSVSEFLDSLDEEILDELNEDAIIIFNKVDNEENCQKISEIGLKT
NAKILPIYKKCNSKGALNKIDPINLNELKEELKNIEVLLIVNENPLDYITEDDLNSIKKIVTISYFKNDIVNKSDYVIPG
KPWAFKEGSYTNSVNTNQNFKIAVPDIVNENLSEIEILMKISEKLDIDL

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin [H]

EC number: 1.2.1.2

Molecular weight: Translated: 41915; Mature: 41915

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLKHTICPSCSTGCGITLISNNKELLGTYPYKRHEINEGKNCKNGRYCFTKYSFNKID
CCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EPSIVNNGKLNESDFKSIIEQFIEDIKNVEPEQFGILCSGNSTNEELDLIKKFSENYGNK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCE
LFLYENGFVNLDKTATSYDDINNSKTILIIGDLYDENPLVARRVIMAKENGAKVICADNE
EEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHHHHEEEEEECCCCEEEEECCC
NKNTTSINSDKYIQFDSVSEFLDSLDEEILDELNEDAIIIFNKVDNEENCQKISEIGLKT
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCC
NAKILPIYKKCNSKGALNKIDPINLNELKEELKNIEVLLIVNENPLDYITEDDLNSIKKI
CCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHH
VTISYFKNDIVNKSDYVIPGKPWAFKEGSYTNSVNTNQNFKIAVPDIVNENLSEIEILMK
HHHHHHHHHHCCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCHHHCCCHHHHHHHHH
ISEKLDIDL
HHHHHCCCC
>Mature Secondary Structure
MLNLKHTICPSCSTGCGITLISNNKELLGTYPYKRHEINEGKNCKNGRYCFTKYSFNKID
CCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EPSIVNNGKLNESDFKSIIEQFIEDIKNVEPEQFGILCSGNSTNEELDLIKKFSENYGNK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCE
LFLYENGFVNLDKTATSYDDINNSKTILIIGDLYDENPLVARRVIMAKENGAKVICADNE
EEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHHHHEEEEEECCCCEEEEECCC
NKNTTSINSDKYIQFDSVSEFLDSLDEEILDELNEDAIIIFNKVDNEENCQKISEIGLKT
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCC
NAKILPIYKKCNSKGALNKIDPINLNELKEELKNIEVLLIVNENPLDYITEDDLNSIKKI
CCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHH
VTISYFKNDIVNKSDYVIPGKPWAFKEGSYTNSVNTNQNFKIAVPDIVNENLSEIEILMK
HHHHHHHHHHCCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCHHHCCCHHHHHHHHH
ISEKLDIDL
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.032 {NAD+}} 0.54 {deamino-NAD+}} 0.19 {3-pyridinecarboxaldehyde-NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]