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The map label for this gene is leuC1 [H]

Identifier: 148643360

GI number: 148643360

Start: 1306940

End: 1308187

Strand: Reverse

Name: leuC1 [H]

Synonym: Msm_1300

Alternate gene names: 148643360

Gene position: 1308187-1306940 (Counterclockwise)

Preceding gene: 148643361

Following gene: 148643359

Centisome position: 70.59

GC content: 37.18

Gene sequence:

>1248_bases
ATGCCAAAAACAATGTCTGAAAAAATATTAGCTAAAGCAGCTGGTAAAGACGAAGTTGAGGCTGGAGATATTGTTATCGC
CAATATTGATGTTGCAATGACTCATGATTTAACCGGACCTCTTTCAGTACAATCTTTTGAGAAAATTGGTGCTACTAAAG
TCTGGGACCCGTCAAAAATTGTAATACCATTCGATCATCAAGTTCCAGCTGATTCTATCGACTCAGCTAACAATCATATT
ATAATGAGAAAATTTGTTAAAGAACAGAAAATTGAAAACTTTTATGATGTTAATGCAGGAGTCTGCCATCAGATTCTTCC
AGAATTAGGACATGTTGTTCCTGGAGAAGTTATTGTTGGTGCAGATTCACATACCTGTACTCATGGAGCTTTAGGTGCGT
TTTCAACAGGAATTGGTTCTACAGATATGGCTATGGTATTTGCAGAAGGTAATTTATGGTTTAAAGTACCTGAAACCAAC
AGATTTGAAATCACCGGTGAATTAAAAGATAATGTTTATGCTAAAGATGTTATTTTAAATATTATCGGCCAGGTAGGCGT
TGACGGTTCAACATATAAAGCCTGTGAATTTGCAGGAGAAACTGTTTCAAATATGAGCATTTCTGACAGAATGGTTTTAA
CAAATATGGCTATTGAAATGGGTGGAAAAACTGGTTTAGTAGAACCTGATAAAAAAACTATTGATTATGTAGAAAGCCGT
TCCAACAAAGCATATAAAGTATTTAAAACTGACTTAGATGCTCCGTCACTTAATATTATTGATATTGACGTAAGTGAGTT
GGAACCGCAAGTAGCCTGTCCTCACCATGTAGACAATGTTAAAGCAGTAAGTGAAGTGGATCAGGAAATTGACCAAGTAT
TCCTCGGATCCTGTACCAATGGTAGGATTAGTGACTTAAGAGATGCTGCTAAAATATTAAAAGGTAAAAAAGTAGCTAAA
GGAACCAGAATGTTAGTTATTCCAGCTTCCAAAGAAGTATACTCTAAAGCACTTGATGAAGGATTACTTAAAATATTTGT
TGATGCAGGAGCTTTAGTATCTGCTCCATGCTGCGGTCCATGTCTTGGAGGACATACCGGAATCATAGGACCTGGAGAAG
TAAGCCTTTCCACATCTAACAGAAACTTTAAAGGCAGACAAGGAAGTCCAGATGGAAAAGTTTACCTGTCTTCTGCTGCA
GTAGCTGCTGCTTCAGCTATTGAAGGAAGAATTGTTGCACCGGAGTGA

Upstream 100 bases:

>100_bases
AATGAAATTTTGAAAAAAAAATAAACAAAGCCCCATTCTAAAATAGTTTATATAGTACAAACATCTATATATTTATCATT
AATATGTGAGGTTTAAAAAT

Downstream 100 bases:

>100_bases
GAATATGAAAGGAACTGTTTGGAAATTCGGAGACGATATTGATACTGACATTATCCTTCCAGGAAGATATTTAATTTATA
CAGATGAAGAAAGATTATCC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MPKTMSEKILAKAAGKDEVEAGDIVIANIDVAMTHDLTGPLSVQSFEKIGATKVWDPSKIVIPFDHQVPADSIDSANNHI
IMRKFVKEQKIENFYDVNAGVCHQILPELGHVVPGEVIVGADSHTCTHGALGAFSTGIGSTDMAMVFAEGNLWFKVPETN
RFEITGELKDNVYAKDVILNIIGQVGVDGSTYKACEFAGETVSNMSISDRMVLTNMAIEMGGKTGLVEPDKKTIDYVESR
SNKAYKVFKTDLDAPSLNIIDIDVSELEPQVACPHHVDNVKAVSEVDQEIDQVFLGSCTNGRISDLRDAAKILKGKKVAK
GTRMLVIPASKEVYSKALDEGLLKIFVDAGALVSAPCCGPCLGGHTGIIGPGEVSLSTSNRNFKGRQGSPDGKVYLSSAA
VAAASAIEGRIVAPE

Sequences:

>Translated_415_residues
MPKTMSEKILAKAAGKDEVEAGDIVIANIDVAMTHDLTGPLSVQSFEKIGATKVWDPSKIVIPFDHQVPADSIDSANNHI
IMRKFVKEQKIENFYDVNAGVCHQILPELGHVVPGEVIVGADSHTCTHGALGAFSTGIGSTDMAMVFAEGNLWFKVPETN
RFEITGELKDNVYAKDVILNIIGQVGVDGSTYKACEFAGETVSNMSISDRMVLTNMAIEMGGKTGLVEPDKKTIDYVESR
SNKAYKVFKTDLDAPSLNIIDIDVSELEPQVACPHHVDNVKAVSEVDQEIDQVFLGSCTNGRISDLRDAAKILKGKKVAK
GTRMLVIPASKEVYSKALDEGLLKIFVDAGALVSAPCCGPCLGGHTGIIGPGEVSLSTSNRNFKGRQGSPDGKVYLSSAA
VAAASAIEGRIVAPE
>Mature_414_residues
PKTMSEKILAKAAGKDEVEAGDIVIANIDVAMTHDLTGPLSVQSFEKIGATKVWDPSKIVIPFDHQVPADSIDSANNHII
MRKFVKEQKIENFYDVNAGVCHQILPELGHVVPGEVIVGADSHTCTHGALGAFSTGIGSTDMAMVFAEGNLWFKVPETNR
FEITGELKDNVYAKDVILNIIGQVGVDGSTYKACEFAGETVSNMSISDRMVLTNMAIEMGGKTGLVEPDKKTIDYVESRS
NKAYKVFKTDLDAPSLNIIDIDVSELEPQVACPHHVDNVKAVSEVDQEIDQVFLGSCTNGRISDLRDAAKILKGKKVAKG
TRMLVIPASKEVYSKALDEGLLKIFVDAGALVSAPCCGPCLGGHTGIIGPGEVSLSTSNRNFKGRQGSPDGKVYLSSAAV
AAASAIEGRIVAPE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=452, Percent_Identity=27.8761061946903, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=36.830835117773, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=25.0712250712251, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI2367097, Length=457, Percent_Identity=24.2888402625821, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=23.9690721649485, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32564738, Length=342, Percent_Identity=30.1169590643275, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=342, Percent_Identity=30.1169590643275, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.2253521126761, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=395, Percent_Identity=22.0253164556962, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=446, Percent_Identity=34.304932735426, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=448, Percent_Identity=32.3660714285714, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323335, Length=449, Percent_Identity=27.1714922048998, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=346, Percent_Identity=27.7456647398844, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=449, Percent_Identity=26.2806236080178, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17864292, Length=449, Percent_Identity=26.2806236080178, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=449, Percent_Identity=26.9487750556793, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161076999, Length=344, Percent_Identity=27.6162790697674, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44231; Mature: 44100

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTMSEKILAKAAGKDEVEAGDIVIANIDVAMTHDLTGPLSVQSFEKIGATKVWDPSKI
CCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCEE
VIPFDHQVPADSIDSANNHIIMRKFVKEQKIENFYDVNAGVCHQILPELGHVVPGEVIVG
EEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
ADSHTCTHGALGAFSTGIGSTDMAMVFAEGNLWFKVPETNRFEITGELKDNVYAKDVILN
CCCCCCCCCCCHHHHCCCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHH
IIGQVGVDGSTYKACEFAGETVSNMSISDRMVLTNMAIEMGGKTGLVEPDKKTIDYVESR
HHHHCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEEHHHEECCCCCCCCCCHHHHHHHHCC
SNKAYKVFKTDLDAPSLNIIDIDVSELEPQVACPHHVDNVKAVSEVDQEIDQVFLGSCTN
CCCEEEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GRISDLRDAAKILKGKKVAKGTRMLVIPASKEVYSKALDEGLLKIFVDAGALVSAPCCGP
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
CLGGHTGIIGPGEVSLSTSNRNFKGRQGSPDGKVYLSSAAVAAASAIEGRIVAPE
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCEEECCC
>Mature Secondary Structure 
PKTMSEKILAKAAGKDEVEAGDIVIANIDVAMTHDLTGPLSVQSFEKIGATKVWDPSKI
CCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCEE
VIPFDHQVPADSIDSANNHIIMRKFVKEQKIENFYDVNAGVCHQILPELGHVVPGEVIVG
EEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
ADSHTCTHGALGAFSTGIGSTDMAMVFAEGNLWFKVPETNRFEITGELKDNVYAKDVILN
CCCCCCCCCCCHHHHCCCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHH
IIGQVGVDGSTYKACEFAGETVSNMSISDRMVLTNMAIEMGGKTGLVEPDKKTIDYVESR
HHHHCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEEHHHEECCCCCCCCCCHHHHHHHHCC
SNKAYKVFKTDLDAPSLNIIDIDVSELEPQVACPHHVDNVKAVSEVDQEIDQVFLGSCTN
CCCEEEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GRISDLRDAAKILKGKKVAKGTRMLVIPASKEVYSKALDEGLLKIFVDAGALVSAPCCGP
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
CLGGHTGIIGPGEVSLSTSNRNFKGRQGSPDGKVYLSSAAVAAASAIEGRIVAPE
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]