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The map label for this gene is leuD [H]

Identifier: 148643359

GI number: 148643359

Start: 1306453

End: 1306935

Strand: Reverse

Name: leuD [H]

Synonym: Msm_1299

Alternate gene names: 148643359

Gene position: 1306935-1306453 (Counterclockwise)

Preceding gene: 148643360

Following gene: 148643358

Centisome position: 70.52

GC content: 32.71

Gene sequence:

>483_bases
ATGAAAGGAACTGTTTGGAAATTCGGAGACGATATTGATACTGACATTATCCTTCCAGGAAGATATTTAATTTATACAGA
TGAAGAAAGATTATCCCAACACTGTATGGAAGGATTAGATGATAAATTTAATGAAAAATGCAAAAAAGGTGACTTTATTG
TAGCTGGCAAAAACTTTGGATGCGGATCTTCCAGAGAACATGCCCCAATAGCTTTAAAAGGTGTTGGAGTAGCTGCAGTA
ATAGCTGAATCATTTGCAAGAATCTTTTACAGAAATGCTACAAATGTTGGAGTTCCACTTCTTGAAGCTCCAGGTATAAG
TAAACTTGTAGAAAATGGAGAAGAAATAGAAGTAGACATGGATAAAGGAACTATAACATCCGAAAATGGAGAAACAATTA
CATTTAAAAAATTACCTCCATTTATGTTAGAAATATTAGAAAAAGGTGGTTTAATTGAGTACCTCAAACAAAAAAGACAA
TAA

Upstream 100 bases:

>100_bases
GGCAGACAAGGAAGTCCAGATGGAAAAGTTTACCTGTCTTCTGCTGCAGTAGCTGCTGCTTCAGCTATTGAAGGAAGAAT
TGTTGCACCGGAGTGAGAAT

Downstream 100 bases:

>100_bases
ATATCAGATAGCTGTTATTCCAGGTGATGGAATAGGTAAAGAAGTTATGGAAGCAACAATCTCTGTTTTAGACGAATTAG
ATGTTGATTTTGATTATATT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKGTVWKFGDDIDTDIILPGRYLIYTDEERLSQHCMEGLDDKFNEKCKKGDFIVAGKNFGCGSSREHAPIALKGVGVAAV
IAESFARIFYRNATNVGVPLLEAPGISKLVENGEEIEVDMDKGTITSENGETITFKKLPPFMLEILEKGGLIEYLKQKRQ

Sequences:

>Translated_160_residues
MKGTVWKFGDDIDTDIILPGRYLIYTDEERLSQHCMEGLDDKFNEKCKKGDFIVAGKNFGCGSSREHAPIALKGVGVAAV
IAESFARIFYRNATNVGVPLLEAPGISKLVENGEEIEVDMDKGTITSENGETITFKKLPPFMLEILEKGGLIEYLKQKRQ
>Mature_160_residues
MKGTVWKFGDDIDTDIILPGRYLIYTDEERLSQHCMEGLDDKFNEKCKKGDFIVAGKNFGCGSSREHAPIALKGVGVAAV
IAESFARIFYRNATNVGVPLLEAPGISKLVENGEEIEVDMDKGTITSENGETITFKKLPPFMLEILEKGGLIEYLKQKRQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=129, Percent_Identity=34.1085271317829, Blast_Score=63, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320440, Length=102, Percent_Identity=44.1176470588235, Blast_Score=88, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=161, Percent_Identity=30.4347826086957, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17760; Mature: 17760

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTVWKFGDDIDTDIILPGRYLIYTDEERLSQHCMEGLDDKFNEKCKKGDFIVAGKNFG
CCCCCCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCC
CGSSREHAPIALKGVGVAAVIAESFARIFYRNATNVGVPLLEAPGISKLVENGEEIEVDM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHCCCCEEEEEC
DKGTITSENGETITFKKLPPFMLEILEKGGLIEYLKQKRQ
CCCEEECCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKGTVWKFGDDIDTDIILPGRYLIYTDEERLSQHCMEGLDDKFNEKCKKGDFIVAGKNFG
CCCCCCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCC
CGSSREHAPIALKGVGVAAVIAESFARIFYRNATNVGVPLLEAPGISKLVENGEEIEVDM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHCCCCEEEEEC
DKGTITSENGETITFKKLPPFMLEILEKGGLIEYLKQKRQ
CCCEEECCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]