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The map label for this gene is leuB [H]

Identifier: 148643358

GI number: 148643358

Start: 1305471

End: 1306481

Strand: Reverse

Name: leuB [H]

Synonym: Msm_1298

Alternate gene names: 148643358

Gene position: 1306481-1305471 (Counterclockwise)

Preceding gene: 148643359

Following gene: 148643357

Centisome position: 70.5

GC content: 35.81

Gene sequence:

>1011_bases
TTGAGTACCTCAAACAAAAAAGACAATAAATATCAGATAGCTGTTATTCCAGGTGATGGAATAGGTAAAGAAGTTATGGA
AGCAACAATCTCTGTTTTAGACGAATTAGATGTTGATTTTGATTATATTTATGGTATAGCAGGTGACGAATGCAATGAAG
AACATGGAACACCTCTTCCTCAGGAAACTATCGATATTGTAAGGGATTCTGATGCATGTTTATTTGGAGCTGCCGGAGAA
ACTGCAGCAGATGTTATTGTAAAAATCCGTCAGGAAATGAAAATGTTTGCTAATTTAAGACCTGTCAAATCTTATCCTAA
CACCAAATCATTATTTGAAAATGTTGACTTTATGATTGTAAGGGAAAATACAGAAGGATTATACATTGCAGACCAGGAAG
AAGAAACCGAAGATGGTGCAATAGCTAAACGTGTTATTACAAGAGAAGCTGAAGAACGTATTATTGATTATGCTTTTCAA
TATGCAAAGGACAACAACAGAACTAAAGTTACTGCAGTTCACAAAGCTAATGTTCTTAAAAAAACCGACGGATTATTTAA
AAAAATATTCTACGAAGTCGGCGAAAAATATCCGGATATTGATACTGAAGACTTTTACGTTGATGCAACAGCAATGTATC
TTGTAACACAGCCTCAGGAATTCCAGGTTGTTGTAACTACCAATTTATTTGGAGATATTTTATCTGATGAAGGTGCAGGA
CTTGTTGGCGGACTTGGTTTAATTCCATCAGCAAATATTGGAGCAGATGGTGCTTTATTTGAACCTGTTCATGGTTCAGC
ACCGGATATTGCAGGTCAGCAAAAAGCAAATCCAATAGCTATGATGCTCTCAGCAATTATGATGCTTAGATACCTTGGTG
AAAATGATGCTGCTGACAAATTTGATGCTGCTATTTTAAAAGTATTAAGTGAAGGTAAAACCTTAACTGGTGATTTAGGC
GGCAGTGCAACTACCATGGAAGTAGCACAAGCTGTTAAAAATGCATTATAA

Upstream 100 bases:

>100_bases
GACATGGATAAAGGAACTATAACATCCGAAAATGGAGAAACAATTACATTTAAAAAATTACCTCCATTTATGTTAGAAAT
ATTAGAAAAAGGTGGTTTAA

Downstream 100 bases:

>100_bases
GAGGAAATCATATGATTAAAGAATCTAATTTAGAAAAAGGCATAAGTGCCATTATCCCAACATACAAGGGAGAAGCGTTT
ATTTCTAAATTATTGGATTC

Product: 3-isopropylmalate dehydrogenase, LeuB

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSTSNKKDNKYQIAVIPGDGIGKEVMEATISVLDELDVDFDYIYGIAGDECNEEHGTPLPQETIDIVRDSDACLFGAAGE
TAADVIVKIRQEMKMFANLRPVKSYPNTKSLFENVDFMIVRENTEGLYIADQEEETEDGAIAKRVITREAEERIIDYAFQ
YAKDNNRTKVTAVHKANVLKKTDGLFKKIFYEVGEKYPDIDTEDFYVDATAMYLVTQPQEFQVVVTTNLFGDILSDEGAG
LVGGLGLIPSANIGADGALFEPVHGSAPDIAGQQKANPIAMMLSAIMMLRYLGENDAADKFDAAILKVLSEGKTLTGDLG
GSATTMEVAQAVKNAL

Sequences:

>Translated_336_residues
MSTSNKKDNKYQIAVIPGDGIGKEVMEATISVLDELDVDFDYIYGIAGDECNEEHGTPLPQETIDIVRDSDACLFGAAGE
TAADVIVKIRQEMKMFANLRPVKSYPNTKSLFENVDFMIVRENTEGLYIADQEEETEDGAIAKRVITREAEERIIDYAFQ
YAKDNNRTKVTAVHKANVLKKTDGLFKKIFYEVGEKYPDIDTEDFYVDATAMYLVTQPQEFQVVVTTNLFGDILSDEGAG
LVGGLGLIPSANIGADGALFEPVHGSAPDIAGQQKANPIAMMLSAIMMLRYLGENDAADKFDAAILKVLSEGKTLTGDLG
GSATTMEVAQAVKNAL
>Mature_335_residues
STSNKKDNKYQIAVIPGDGIGKEVMEATISVLDELDVDFDYIYGIAGDECNEEHGTPLPQETIDIVRDSDACLFGAAGET
AADVIVKIRQEMKMFANLRPVKSYPNTKSLFENVDFMIVRENTEGLYIADQEEETEDGAIAKRVITREAEERIIDYAFQY
AKDNNRTKVTAVHKANVLKKTDGLFKKIFYEVGEKYPDIDTEDFYVDATAMYLVTQPQEFQVVVTTNLFGDILSDEGAGL
VGGLGLIPSANIGADGALFEPVHGSAPDIAGQQKANPIAMMLSAIMMLRYLGENDAADKFDAAILKVLSEGKTLTGDLGG
SATTMEVAQAVKNAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=39.8826979472141, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=35.9882005899705, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI28178816, Length=341, Percent_Identity=35.7771260997067, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI28178838, Length=313, Percent_Identity=36.1022364217252, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI28178821, Length=337, Percent_Identity=35.3115727002967, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI28178819, Length=191, Percent_Identity=42.4083769633508, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI87081683, Length=346, Percent_Identity=37.8612716763006, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1788101, Length=351, Percent_Identity=37.3219373219373, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787381, Length=363, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=39.1176470588235, Blast_Score=246, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=35.7988165680473, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=34.4213649851632, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17505779, Length=246, Percent_Identity=39.4308943089431, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=41.8994413407821, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=37.8299120234604, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324291, Length=341, Percent_Identity=36.6568914956012, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=33.5227272727273, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24643270, Length=325, Percent_Identity=43.6923076923077, Blast_Score=270, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24643268, Length=330, Percent_Identity=43.030303030303, Blast_Score=270, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=37.4251497005988, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI161078635, Length=332, Percent_Identity=32.5301204819277, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI161078633, Length=332, Percent_Identity=32.5301204819277, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24650122, Length=332, Percent_Identity=32.5301204819277, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI161078637, Length=332, Percent_Identity=32.5301204819277, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=34.1176470588235, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=34.1176470588235, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI161078639, Length=248, Percent_Identity=37.0967741935484, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI20130355, Length=334, Percent_Identity=26.9461077844311, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550436, Length=251, Percent_Identity=21.9123505976096, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36609; Mature: 36478

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSNKKDNKYQIAVIPGDGIGKEVMEATISVLDELDVDFDYIYGIAGDECNEEHGTPLP
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCC
QETIDIVRDSDACLFGAAGETAADVIVKIRQEMKMFANLRPVKSYPNTKSLFENVDFMIV
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEE
RENTEGLYIADQEEETEDGAIAKRVITREAEERIIDYAFQYAKDNNRTKVTAVHKANVLK
EECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
KTDGLFKKIFYEVGEKYPDIDTEDFYVDATAMYLVTQPQEFQVVVTTNLFGDILSDEGAG
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCEEEEEEECHHHHHHCCCCCC
LVGGLGLIPSANIGADGALFEPVHGSAPDIAGQQKANPIAMMLSAIMMLRYLGENDAADK
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
FDAAILKVLSEGKTLTGDLGGSATTMEVAQAVKNAL
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STSNKKDNKYQIAVIPGDGIGKEVMEATISVLDELDVDFDYIYGIAGDECNEEHGTPLP
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCC
QETIDIVRDSDACLFGAAGETAADVIVKIRQEMKMFANLRPVKSYPNTKSLFENVDFMIV
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEE
RENTEGLYIADQEEETEDGAIAKRVITREAEERIIDYAFQYAKDNNRTKVTAVHKANVLK
EECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
KTDGLFKKIFYEVGEKYPDIDTEDFYVDATAMYLVTQPQEFQVVVTTNLFGDILSDEGAG
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCEEEEEEECHHHHHHCCCCCC
LVGGLGLIPSANIGADGALFEPVHGSAPDIAGQQKANPIAMMLSAIMMLRYLGENDAADK
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
FDAAILKVLSEGKTLTGDLGGSATTMEVAQAVKNAL
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]