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The map label for this gene is hyfB [C]

Identifier: 148643131

GI number: 148643131

Start: 1026714

End: 1028171

Strand: Reverse

Name: hyfB [C]

Synonym: Msm_1071

Alternate gene names: 148643131

Gene position: 1028171-1026714 (Counterclockwise)

Preceding gene: 148643132

Following gene: 148643130

Centisome position: 55.48

GC content: 33.74

Gene sequence:

>1458_bases
ATGAGTAACTATTTGATTCCTCTTATGGTAGTAGTTCCTATGCTTTGTGCAATACTTGTAAATGCTTTTGCTAAATTTAA
TAAATCTATTAAAGTATTAACATTTGTAGTTGCTATCTGTTTACCGATTATTCCATTGCTTTCCAATTATGGATTGCACT
TCTTTGGAGGATATACTCCGATGTTGGATAATGCAACTGGTGTAATTTATCATCCGGCTATTACTTATGCTTTTGACATG
CTGCAACAGATATTCATAGCTGCAGTAGGTTTAATAACATTTTTAGTAGCTTTTATTTACTTGTCAAAATATAAAAAAGC
ATCCGGACAGTATTTGTTCTTATTGTTCATGGGTACAGCATCTGTAACTGCATTAATGCTTACTGATGATATATTTAACA
TGTTTGTATTCTTTGAGATACTTGCATTGGTTCAAGTAGGTATTGTAATTGCGTCTCCTATGGATTACAGTTATGAAATG
GCTCTTAAATATATGATTTTAGGATCTATTGGAAGTCCGATAATGCTTTTAGGAATCGGATTCTTACTTGCAAGTACTGG
TAATATTAACATTTCTGATATAATTGTGGCTCTTCACAATGGAACTTTAAGTTATACCTCCCCAGTATTTTTAATGTCAT
TTGCTTTAATATTCTTCGGATGGTTATATGCTTCAGGTTTGCCTCCGTTCCATATTATAAAATCCGGAATGTACAGTAAA
GCAGAACCTCATGGTGCAGCTTTGCTTCAAACATTTACGGTTATTTCAATGATTTCAATTGTATTGATAATGTTTAGAAT
ATTCCATGAATTGCCAATATTTGAACTGTTATTGGTTTTGTTTTCAGTATTGGCTATGATTCTAGGTGTTTCACTGGCTT
TAACTCAAACAGACTTCAGAAGAATGATTGGTTATTTGGCAGTTGGTGAGCTTGGTTTTATCGGTTTAGGTATTGGTCTT
GGAACTCAATTTGCAATTACTGCAGGTCTTTTCCAGGCACTTAATGAAATAATTACTACTGCATTATTATTTATAGGATT
CGGACTTATTGCTTATGTAACTAAAGAGGAAGACACCCGTAAATTGGGAGGATTGCTTGCTTATCATCCGAAAGTTGGTT
TAATGCTTTTATTTGGTGGTTTGGCAATGGCTGGTGTTCCTCCATTCAGCGGATTCCAAAGTAAATTAATGCTTGTTCAA
GCTTCATTAAGTTGCGGATATCCGGAATTATCCATTCTTGCGGTTATAGTAAGTATAGCTACATTTGTTGTATTTGTTAA
AACATTCTATGGAATGTTTTTAAGACCAAAACCAAAAGGTTTGGAAGTCCCTGACAAGGAGGTTCCTAAATCAGCAGTAT
TTGCAATGGTTGTTTTATTGGCTTTAATTATTATATTGGGTCTTTGCCCAGGTCTTGTTACAAATGGAATTTATGAGTTT
GTAGGAGGTATTTTATGA

Upstream 100 bases:

>100_bases
TTGGCTGTAATGTTGGCTATTGTAATGGTTATTTACAAGAAATACGGAACTTTAAGTACAAAAGTACTGTTGGCTGATAC
ATCTAAACAGGAGGAATACG

Downstream 100 bases:

>100_bases
AACTTTATGATCAAATTTTCAATGTTGTAAAGGATTTTAGAAAATTCTTTTCTCCAGGTCCTGTAACTAATGCAGATGTT
TCTGGAAGTATTACATCTGA

Product: hydrogenase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSNYLIPLMVVVPMLCAILVNAFAKFNKSIKVLTFVVAICLPIIPLLSNYGLHFFGGYTPMLDNATGVIYHPAITYAFDM
LQQIFIAAVGLITFLVAFIYLSKYKKASGQYLFLLFMGTASVTALMLTDDIFNMFVFFEILALVQVGIVIASPMDYSYEM
ALKYMILGSIGSPIMLLGIGFLLASTGNINISDIIVALHNGTLSYTSPVFLMSFALIFFGWLYASGLPPFHIIKSGMYSK
AEPHGAALLQTFTVISMISIVLIMFRIFHELPIFELLLVLFSVLAMILGVSLALTQTDFRRMIGYLAVGELGFIGLGIGL
GTQFAITAGLFQALNEIITTALLFIGFGLIAYVTKEEDTRKLGGLLAYHPKVGLMLLFGGLAMAGVPPFSGFQSKLMLVQ
ASLSCGYPELSILAVIVSIATFVVFVKTFYGMFLRPKPKGLEVPDKEVPKSAVFAMVVLLALIIILGLCPGLVTNGIYEF
VGGIL

Sequences:

>Translated_485_residues
MSNYLIPLMVVVPMLCAILVNAFAKFNKSIKVLTFVVAICLPIIPLLSNYGLHFFGGYTPMLDNATGVIYHPAITYAFDM
LQQIFIAAVGLITFLVAFIYLSKYKKASGQYLFLLFMGTASVTALMLTDDIFNMFVFFEILALVQVGIVIASPMDYSYEM
ALKYMILGSIGSPIMLLGIGFLLASTGNINISDIIVALHNGTLSYTSPVFLMSFALIFFGWLYASGLPPFHIIKSGMYSK
AEPHGAALLQTFTVISMISIVLIMFRIFHELPIFELLLVLFSVLAMILGVSLALTQTDFRRMIGYLAVGELGFIGLGIGL
GTQFAITAGLFQALNEIITTALLFIGFGLIAYVTKEEDTRKLGGLLAYHPKVGLMLLFGGLAMAGVPPFSGFQSKLMLVQ
ASLSCGYPELSILAVIVSIATFVVFVKTFYGMFLRPKPKGLEVPDKEVPKSAVFAMVVLLALIIILGLCPGLVTNGIYEF
VGGIL
>Mature_484_residues
SNYLIPLMVVVPMLCAILVNAFAKFNKSIKVLTFVVAICLPIIPLLSNYGLHFFGGYTPMLDNATGVIYHPAITYAFDML
QQIFIAAVGLITFLVAFIYLSKYKKASGQYLFLLFMGTASVTALMLTDDIFNMFVFFEILALVQVGIVIASPMDYSYEMA
LKYMILGSIGSPIMLLGIGFLLASTGNINISDIIVALHNGTLSYTSPVFLMSFALIFFGWLYASGLPPFHIIKSGMYSKA
EPHGAALLQTFTVISMISIVLIMFRIFHELPIFELLLVLFSVLAMILGVSLALTQTDFRRMIGYLAVGELGFIGLGIGLG
TQFAITAGLFQALNEIITTALLFIGFGLIAYVTKEEDTRKLGGLLAYHPKVGLMLLFGGLAMAGVPPFSGFQSKLMLVQA
SLSCGYPELSILAVIVSIATFVVFVKTFYGMFLRPKPKGLEVPDKEVPKSAVFAMVVLLALIIILGLCPGLVTNGIYEFV
GGIL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788827, Length=401, Percent_Identity=25.6857855361596, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=24.5179063360882, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=419, Percent_Identity=23.1503579952267, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788829, Length=369, Percent_Identity=25.4742547425474, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788613, Length=480, Percent_Identity=22.7083333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI2367154, Length=136, Percent_Identity=30.1470588235294, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 52685; Mature: 52553

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYLIPLMVVVPMLCAILVNAFAKFNKSIKVLTFVVAICLPIIPLLSNYGLHFFGGYTP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCC
MLDNATGVIYHPAITYAFDMLQQIFIAAVGLITFLVAFIYLSKYKKASGQYLFLLFMGTA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHH
SVTALMLTDDIFNMFVFFEILALVQVGIVIASPMDYSYEMALKYMILGSIGSPIMLLGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
FLLASTGNINISDIIVALHNGTLSYTSPVFLMSFALIFFGWLYASGLPPFHIIKSGMYSK
HHHHCCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
AEPHGAALLQTFTVISMISIVLIMFRIFHELPIFELLLVLFSVLAMILGVSLALTQTDFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMIGYLAVGELGFIGLGIGLGTQFAITAGLFQALNEIITTALLFIGFGLIAYVTKEEDTR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KLGGLLAYHPKVGLMLLFGGLAMAGVPPFSGFQSKLMLVQASLSCGYPELSILAVIVSIA
HHCCCCEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TFVVFVKTFYGMFLRPKPKGLEVPDKEVPKSAVFAMVVLLALIIILGLCPGLVTNGIYEF
HHHHHHHHHHHHEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGIL
HCCCC
>Mature Secondary Structure 
SNYLIPLMVVVPMLCAILVNAFAKFNKSIKVLTFVVAICLPIIPLLSNYGLHFFGGYTP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCC
MLDNATGVIYHPAITYAFDMLQQIFIAAVGLITFLVAFIYLSKYKKASGQYLFLLFMGTA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHH
SVTALMLTDDIFNMFVFFEILALVQVGIVIASPMDYSYEMALKYMILGSIGSPIMLLGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
FLLASTGNINISDIIVALHNGTLSYTSPVFLMSFALIFFGWLYASGLPPFHIIKSGMYSK
HHHHCCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
AEPHGAALLQTFTVISMISIVLIMFRIFHELPIFELLLVLFSVLAMILGVSLALTQTDFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMIGYLAVGELGFIGLGIGLGTQFAITAGLFQALNEIITTALLFIGFGLIAYVTKEEDTR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KLGGLLAYHPKVGLMLLFGGLAMAGVPPFSGFQSKLMLVQASLSCGYPELSILAVIVSIA
HHCCCCEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TFVVFVKTFYGMFLRPKPKGLEVPDKEVPKSAVFAMVVLLALIIILGLCPGLVTNGIYEF
HHHHHHHHHHHHEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGIL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]