Click here to switch to the map view.

The map label for this gene is queD [H]

Identifier: 148643116

GI number: 148643116

Start: 1016657

End: 1017178

Strand: Reverse

Name: queD [H]

Synonym: Msm_1056

Alternate gene names: 148643116

Gene position: 1017178-1016657 (Counterclockwise)

Preceding gene: 148643117

Following gene: 148643115

Centisome position: 54.89

GC content: 31.23

Gene sequence:

>522_bases
ATGAAAATTATGATTAACGGTATACAATCAAATTTAAGATTCTCTTCAGCTCATGTTATTCCGGGACATGAATCATGCGG
TTATATTCATGGACATTCCTATTTTGTAGATGTGGAAATCGAAGGAGAACGTGCAGGGGATTTTGAATTTGTAGTGGACT
TTAAAGATGTCAAAGCATACACTAAAGCAATTTGTAATGAATTGGACCACAGGTTATTAATTCCTGTTTACAATGATTTA
ATAGATATTAAAGATTTTAATAAAAAATCAGACTCTATTTTCGATTTAAAAGAAGAAAAAACAGTTCATTTTAAAATAGC
TGGAAAAGGATATTCTATTCCTGGTGAAGATTGTGTTTTCTTACCTCTCCCTTATTCTTCTGCTGAAGAGTTGTCCAAAT
TTTTTGCTGAAACATTAACTAAAAAATTAGCTGAAAAATATGATAATCTGGAATATGTTGCAGTTGGAGTTAATGAGGGA
ATCGGACAGGGAGCAATTTATAAAAAAGTGCTTGATGATTAG

Upstream 100 bases:

>100_bases
AACTGAATTGGTTAATGATGTTGCTGGCAGATATATTGATGAATTAGAAACAATTGAAAAAGATATTAGCAAAACTAACT
TATTATAGAATGGTGATTTT

Downstream 100 bases:

>100_bases
TTATGAAAGCTCCAATAATCGAAATTTTTTCAAGTTTTCAGGGGGAAGGCCTTTTCATTGGTCAAAGACAAATCTTTGTT
AGATTTGCAGGATGTAACTT

Product: 6-pyruvoyl-tetrahydropterin synthase

Products: 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine; Triphosphate. [C]

Alternate protein names: CPH4 synthase; Archaeosine biosynthesis protein queD [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKIMINGIQSNLRFSSAHVIPGHESCGYIHGHSYFVDVEIEGERAGDFEFVVDFKDVKAYTKAICNELDHRLLIPVYNDL
IDIKDFNKKSDSIFDLKEEKTVHFKIAGKGYSIPGEDCVFLPLPYSSAEELSKFFAETLTKKLAEKYDNLEYVAVGVNEG
IGQGAIYKKVLDD

Sequences:

>Translated_173_residues
MKIMINGIQSNLRFSSAHVIPGHESCGYIHGHSYFVDVEIEGERAGDFEFVVDFKDVKAYTKAICNELDHRLLIPVYNDL
IDIKDFNKKSDSIFDLKEEKTVHFKIAGKGYSIPGEDCVFLPLPYSSAEELSKFFAETLTKKLAEKYDNLEYVAVGVNEG
IGQGAIYKKVLDD
>Mature_173_residues
MKIMINGIQSNLRFSSAHVIPGHESCGYIHGHSYFVDVEIEGERAGDFEFVVDFKDVKAYTKAICNELDHRLLIPVYNDL
IDIKDFNKKSDSIFDLKEEKTVHFKIAGKGYSIPGEDCVFLPLPYSSAEELSKFFAETLTKKLAEKYDNLEYVAVGVNEG
IGQGAIYKKVLDD

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12 [C]

Molecular weight: Translated: 19508; Mature: 19508

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMINGIQSNLRFSSAHVIPGHESCGYIHGHSYFVDVEIEGERAGDFEFVVDFKDVKAY
CEEEEECCCCCCEECCEEEECCCCCCCEEECCEEEEEEEECCCCCCCEEEEEEHHHHHHH
TKAICNELDHRLLIPVYNDLIDIKDFNKKSDSIFDLKEEKTVHFKIAGKGYSIPGEDCVF
HHHHHHHCCCEEEEEHHHCEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEE
LPLPYSSAEELSKFFAETLTKKLAEKYDNLEYVAVGVNEGIGQGAIYKKVLDD
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKIMINGIQSNLRFSSAHVIPGHESCGYIHGHSYFVDVEIEGERAGDFEFVVDFKDVKAY
CEEEEECCCCCCEECCEEEECCCCCCCEEECCEEEEEEEECCCCCCCEEEEEEHHHHHHH
TKAICNELDHRLLIPVYNDLIDIKDFNKKSDSIFDLKEEKTVHFKIAGKGYSIPGEDCVF
HHHHHHHCCCEEEEEHHHCEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEE
LPLPYSSAEELSKFFAETLTKKLAEKYDNLEYVAVGVNEGIGQGAIYKKVLDD
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate [C]

Specific reaction: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate = 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine + Triphosphate. [C]

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]