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The map label for this gene is pyrK [H]

Identifier: 148643103

GI number: 148643103

Start: 1006012

End: 1006818

Strand: Reverse

Name: pyrK [H]

Synonym: Msm_1043

Alternate gene names: 148643103

Gene position: 1006818-1006012 (Counterclockwise)

Preceding gene: 148643104

Following gene: 148643102

Centisome position: 54.33

GC content: 34.2

Gene sequence:

>807_bases
ATGAATGATGTTCCACAAATTGTTGAAATTAATGAAATAATTGAAGAGACTCCAACAATTAAAACTTTTGTCTTTGATTG
GGATATGGATAAGTTCGGTGTTCCTTCTCCGGGGGAATTTGTGATGGTTTGGAATTTCCATAATGAAAAACCAATGTCCA
TTTCTTTAATTGACAAAGAAAATTCCAGAATGGCCATTAGTGTTAAAAATGTTGGTGAATTTTCATCACAGCTTCATGAT
TTAAAAGTTGGAGATAAAATAGGTATTCGAGGAAGCTATGGAAATGGATTTTCAAATGATTTAACTGATAAAAAAGTTTT
AGCTATTGGTGGTGGAGTTGGAATGGCTCCTATTAATGCAATAGTTAGTGATTTGCTTAAAAAAGGCAATGCTGTTGATG
TAGTTCCGGCAGCCGTTACAAAAGAAGAATTGCTGTTTATGGAGTCATTGGAAAAATCAGGTGCAAAGATTCATCCGTGT
ACTGATGATGGAAGTTTTGGATTTGAAGGATTTGCAACAGATTGTACTGTAAGTTTACTTGAAGATAACTCTTATGATTT
TGCATTTGTTTGTGGTCCTGAGATAATGATGAAAGGAATCTATGAAATACTTGAAGATGCTGGAATAAGTGCAGAATATT
CTCTAGAGAGATACATGAAATGTGCTCTAGGTATATGTGGTCAGTGTTGTGTTGATAATACTGGATGGAGAATCTGTGTA
GAAGGGCCTGTTTTTAAAAATGAGAAAATAGAAGAAATTTCAGAGTTCGGTAAATACAGAAGGAATGCTGCAGGAATAAA
GCAGTAA

Upstream 100 bases:

>100_bases
ATTTGGCAAAATCAACAAAGATTTAGAAGAATTTATGAATAAAAAAGGTTATGAATCTATTGATGAAATGATTGGCCTTG
CACATGAGGAGTTGTAATTT

Downstream 100 bases:

>100_bases
TTAAATGTGATTCTAATGGAAAAAGACTTAAAAGAATATATAACTCCTCCAGTATTGCTGGTACTGTTCTTGCTTGGAAT
ATCACTAATGATTGTATCTC

Product: dihydroorotate dehydrogenase electron transfer subunit

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNDVPQIVEINEIIEETPTIKTFVFDWDMDKFGVPSPGEFVMVWNFHNEKPMSISLIDKENSRMAISVKNVGEFSSQLHD
LKVGDKIGIRGSYGNGFSNDLTDKKVLAIGGGVGMAPINAIVSDLLKKGNAVDVVPAAVTKEELLFMESLEKSGAKIHPC
TDDGSFGFEGFATDCTVSLLEDNSYDFAFVCGPEIMMKGIYEILEDAGISAEYSLERYMKCALGICGQCCVDNTGWRICV
EGPVFKNEKIEEISEFGKYRRNAAGIKQ

Sequences:

>Translated_268_residues
MNDVPQIVEINEIIEETPTIKTFVFDWDMDKFGVPSPGEFVMVWNFHNEKPMSISLIDKENSRMAISVKNVGEFSSQLHD
LKVGDKIGIRGSYGNGFSNDLTDKKVLAIGGGVGMAPINAIVSDLLKKGNAVDVVPAAVTKEELLFMESLEKSGAKIHPC
TDDGSFGFEGFATDCTVSLLEDNSYDFAFVCGPEIMMKGIYEILEDAGISAEYSLERYMKCALGICGQCCVDNTGWRICV
EGPVFKNEKIEEISEFGKYRRNAAGIKQ
>Mature_268_residues
MNDVPQIVEINEIIEETPTIKTFVFDWDMDKFGVPSPGEFVMVWNFHNEKPMSISLIDKENSRMAISVKNVGEFSSQLHD
LKVGDKIGIRGSYGNGFSNDLTDKKVLAIGGGVGMAPINAIVSDLLKKGNAVDVVPAAVTKEELLFMESLEKSGAKIHPC
TDDGSFGFEGFATDCTVSLLEDNSYDFAFVCGPEIMMKGIYEILEDAGISAEYSLERYMKCALGICGQCCVDNTGWRICV
EGPVFKNEKIEEISEFGKYRRNAAGIKQ

Specific function: Is responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the pyrD subunit to the ultimate electron acceptor NAD(+) [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 29473; Mature: 29473

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00197 2FE2S_FER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVPQIVEINEIIEETPTIKTFVFDWDMDKFGVPSPGEFVMVWNFHNEKPMSISLIDKE
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECC
NSRMAISVKNVGEFSSQLHDLKVGDKIGIRGSYGNGFSNDLTDKKVLAIGGGVGMAPINA
CCEEEEEECCHHHHHHHHHHCCCCCEECEECCCCCCCCCCCCCCEEEEECCCCCHHHHHH
IVSDLLKKGNAVDVVPAAVTKEELLFMESLEKSGAKIHPCTDDGSFGFEGFATDCTVSLL
HHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEE
EDNSYDFAFVCGPEIMMKGIYEILEDAGISAEYSLERYMKCALGICGQCCVDNTGWRICV
ECCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
EGPVFKNEKIEEISEFGKYRRNAAGIKQ
CCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDVPQIVEINEIIEETPTIKTFVFDWDMDKFGVPSPGEFVMVWNFHNEKPMSISLIDKE
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECC
NSRMAISVKNVGEFSSQLHDLKVGDKIGIRGSYGNGFSNDLTDKKVLAIGGGVGMAPINA
CCEEEEEECCHHHHHHHHHHCCCCCEECEECCCCCCCCCCCCCCEEEEECCCCCHHHHHH
IVSDLLKKGNAVDVVPAAVTKEELLFMESLEKSGAKIHPCTDDGSFGFEGFATDCTVSLL
HHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEE
EDNSYDFAFVCGPEIMMKGIYEILEDAGISAEYSLERYMKCALGICGQCCVDNTGWRICV
ECCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
EGPVFKNEKIEEISEFGKYRRNAAGIKQ
CCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]