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The map label for this gene is mcrB [H]

Identifier: 148643079

GI number: 148643079

Start: 981008

End: 982339

Strand: Reverse

Name: mcrB [H]

Synonym: Msm_1019

Alternate gene names: 148643079

Gene position: 982339-981008 (Counterclockwise)

Preceding gene: 148643081

Following gene: 148643078

Centisome position: 53.01

GC content: 37.69

Gene sequence:

>1332_bases
ATGGCAAAGTTTGATGATAAAGTCGATTTATACGACGATAGAGGCTCATTAGTTGTATCTGATGTTCCAATCGAAGCTTT
AAGTCCACTCAGGAACACTGCTATTCAAAACATCGTTAAAGGTGTAAAAAGAACTGTTGCAGTAAACTTAGAAGGTCTTG
AAAAATCTGTCAAAACTGGTTCAGTCGGTGGAGACAAATCCAAAATATTAGGAAGGGAATTAGATATTGATATTGTAGCA
AATGCGGGTGCAATTGCAGAAAAAATGAAAGAAATGATACAAATTTCTGAAGATGATGATACTAAAGTTGAACCTATTTC
CGGAGGTAAAAGATTATTAGTACAAGTTCCATCTAAAAGAATTGATGTTGCTGCAGAATACTCTGTTGCTCCATTATCTA
CCGCAACTTCATTAGTACAAGCTATTATTGATGTATGTGACGTAAGTATCTATGATGCAAACTTCGTAAAAGCTGCAGTA
TTAGGTAGATGCCCACAATCTGTAGATTACAAAGGATCCAACATTGCTACTATGTTAGACATTCCACAAAAACTCGAAGG
TGCAGGTTATGCATTAAGAGGTGTTAAAGCAAATGATTTTGCTGCTGCTACCTTAAAAAACACTTTCCAAGCTACTGCTT
TAGCATCTATTTTCGAACAAACTGCTATGTTTGAAATGGGTGATGCTATTGGTGCATACGAAAGATTACACTTATTAGGT
TTAGCTTACCAAGGTATGAATGCTGACAATATGGTTTTAGATTTAGTAAAAGATAATGCAAAAGAAGGTACTGTTGGTAG
TGTTGTAAATGGAACTATCGCTAGAGCTGAAGCTGACGGAGTTATTGCTCCTCAAAAAGACTTAACCGATTTCTCAATTT
ATAACACTGATGACGCAGCTTTATGGAACGCATATGCTGCTGCTGGTGCTGCTGCTGCTGTTATGGTAAACATTGGTGCT
GCTCGTGCTGCTCAAGGTATTCCATCCACTTTATTATACTTCAATGATAACATTGAATTTGCTACTGGTTTACCAAGTAT
TGACTACGGTAGAGCAGAAGGTGTAGCTGTAGGATTCTCTTTCTTCAGTCACTCTATCTACGGTGGAGGAGGCCCTGGTC
TCTTTAACGGTAACCACGTTGTAACCAGACACAGTAAAGGATTCTGTATCCCTTGTGTAGCTGCTGCTATGTCCTTAGAT
GCAGGAACACAACTCTTTTCACCAGAAGCAACTTCTGGTTTAATTAAAGAAGTATACAGTCAAGTTGATGAATTTAGAGA
ACCTCTCAAATATGTTGCTTTAGCAGCTGATGATATTAAAGGTGACATCTAA

Upstream 100 bases:

>100_bases
TCTACAAAATTATTGTGTATAGCTTTATCCAATTTTGTGTGAAAATTTCACTCAAATATAGGTGGTATATTAAATACTCT
ATTTAATTAGGAGGGAAAAA

Downstream 100 bases:

>100_bases
TTAATTTAATATTTAAATCTAATTTTTCATGGACGGCAAAAAAATGGATATTGAAATATTCCCATACCGAGTGCTTGGAA
GTGACACAACAGAAAAGTTG

Product: methyl-coenzyme M reductase, beta subunit, McrB

Products: NA

Alternate protein names: MCR I beta; Coenzyme-B sulfoethylthiotransferase beta [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAKFDDKVDLYDDRGSLVVSDVPIEALSPLRNTAIQNIVKGVKRTVAVNLEGLEKSVKTGSVGGDKSKILGRELDIDIVA
NAGAIAEKMKEMIQISEDDDTKVEPISGGKRLLVQVPSKRIDVAAEYSVAPLSTATSLVQAIIDVCDVSIYDANFVKAAV
LGRCPQSVDYKGSNIATMLDIPQKLEGAGYALRGVKANDFAAATLKNTFQATALASIFEQTAMFEMGDAIGAYERLHLLG
LAYQGMNADNMVLDLVKDNAKEGTVGSVVNGTIARAEADGVIAPQKDLTDFSIYNTDDAALWNAYAAAGAAAAVMVNIGA
ARAAQGIPSTLLYFNDNIEFATGLPSIDYGRAEGVAVGFSFFSHSIYGGGGPGLFNGNHVVTRHSKGFCIPCVAAAMSLD
AGTQLFSPEATSGLIKEVYSQVDEFREPLKYVALAADDIKGDI

Sequences:

>Translated_443_residues
MAKFDDKVDLYDDRGSLVVSDVPIEALSPLRNTAIQNIVKGVKRTVAVNLEGLEKSVKTGSVGGDKSKILGRELDIDIVA
NAGAIAEKMKEMIQISEDDDTKVEPISGGKRLLVQVPSKRIDVAAEYSVAPLSTATSLVQAIIDVCDVSIYDANFVKAAV
LGRCPQSVDYKGSNIATMLDIPQKLEGAGYALRGVKANDFAAATLKNTFQATALASIFEQTAMFEMGDAIGAYERLHLLG
LAYQGMNADNMVLDLVKDNAKEGTVGSVVNGTIARAEADGVIAPQKDLTDFSIYNTDDAALWNAYAAAGAAAAVMVNIGA
ARAAQGIPSTLLYFNDNIEFATGLPSIDYGRAEGVAVGFSFFSHSIYGGGGPGLFNGNHVVTRHSKGFCIPCVAAAMSLD
AGTQLFSPEATSGLIKEVYSQVDEFREPLKYVALAADDIKGDI
>Mature_442_residues
AKFDDKVDLYDDRGSLVVSDVPIEALSPLRNTAIQNIVKGVKRTVAVNLEGLEKSVKTGSVGGDKSKILGRELDIDIVAN
AGAIAEKMKEMIQISEDDDTKVEPISGGKRLLVQVPSKRIDVAAEYSVAPLSTATSLVQAIIDVCDVSIYDANFVKAAVL
GRCPQSVDYKGSNIATMLDIPQKLEGAGYALRGVKANDFAAATLKNTFQATALASIFEQTAMFEMGDAIGAYERLHLLGL
AYQGMNADNMVLDLVKDNAKEGTVGSVVNGTIARAEADGVIAPQKDLTDFSIYNTDDAALWNAYAAAGAAAAVMVNIGAA
RAAQGIPSTLLYFNDNIEFATGLPSIDYGRAEGVAVGFSFFSHSIYGGGGPGLFNGNHVVTRHSKGFCIPCVAAAMSLDA
GTQLFSPEATSGLIKEVYSQVDEFREPLKYVALAADDIKGDI

Specific function: Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide [H]

COG id: COG4054

COG function: function code H; Methyl coenzyme M reductase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022681
- InterPro:   IPR008924
- InterPro:   IPR015823
- InterPro:   IPR003179
- InterPro:   IPR022679
- InterPro:   IPR022680
- InterPro:   IPR009024 [H]

Pfam domain/function: PF02241 MCR_beta; PF02783 MCR_beta_N [H]

EC number: =2.8.4.1 [H]

Molecular weight: Translated: 46740; Mature: 46609

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFDDKVDLYDDRGSLVVSDVPIEALSPLRNTAIQNIVKGVKRTVAVNLEGLEKSVKTG
CCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCC
SVGGDKSKILGRELDIDIVANAGAIAEKMKEMIQISEDDDTKVEPISGGKRLLVQVPSKR
CCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCC
IDVAAEYSVAPLSTATSLVQAIIDVCDVSIYDANFVKAAVLGRCPQSVDYKGSNIATMLD
CCEEECCCCCCHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEC
IPQKLEGAGYALRGVKANDFAAATLKNTFQATALASIFEQTAMFEMGDAIGAYERLHLLG
CCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYQGMNADNMVLDLVKDNAKEGTVGSVVNGTIARAEADGVIAPQKDLTDFSIYNTDDAA
HHCCCCCCCHHHHEEHHCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCHH
LWNAYAAAGAAAAVMVNIGAARAAQGIPSTLLYFNDNIEFATGLPSIDYGRAEGVAVGFS
HHHHHHHHHHHEEHEEECCHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCCEEEEHH
FFSHSIYGGGGPGLFNGNHVVTRHSKGFCIPCVAAAMSLDAGTQLFSPEATSGLIKEVYS
HHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
QVDEFREPLKYVALAADDIKGDI
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKFDDKVDLYDDRGSLVVSDVPIEALSPLRNTAIQNIVKGVKRTVAVNLEGLEKSVKTG
CCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCC
SVGGDKSKILGRELDIDIVANAGAIAEKMKEMIQISEDDDTKVEPISGGKRLLVQVPSKR
CCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCC
IDVAAEYSVAPLSTATSLVQAIIDVCDVSIYDANFVKAAVLGRCPQSVDYKGSNIATMLD
CCEEECCCCCCHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEC
IPQKLEGAGYALRGVKANDFAAATLKNTFQATALASIFEQTAMFEMGDAIGAYERLHLLG
CCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYQGMNADNMVLDLVKDNAKEGTVGSVVNGTIARAEADGVIAPQKDLTDFSIYNTDDAA
HHCCCCCCCHHHHEEHHCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCHH
LWNAYAAAGAAAAVMVNIGAARAAQGIPSTLLYFNDNIEFATGLPSIDYGRAEGVAVGFS
HHHHHHHHHHHEEHEEECCHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCCEEEEHH
FFSHSIYGGGGPGLFNGNHVVTRHSKGFCIPCVAAAMSLDAGTQLFSPEATSGLIKEVYS
HHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
QVDEFREPLKYVALAADDIKGDI
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929010; 9371463; 2269306 [H]