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The map label for this gene is prf1

Identifier: 148642951

GI number: 148642951

Start: 852580

End: 853818

Strand: Reverse

Name: prf1

Synonym: Msm_0891

Alternate gene names: 148642951

Gene position: 853818-852580 (Counterclockwise)

Preceding gene: 148642953

Following gene: 148642948

Centisome position: 46.07

GC content: 33.58

Gene sequence:

>1239_bases
ATGGCAGAAGTATCATCAAAAGAATTATATAAATTTAAAAAAACTTTAAAAGAGTTATCAGAGAAAAAAGGTAGAGGTAC
AGAGCTTGTTTCCGTTTATATTCCTCATGATAAACAAATTAGTGATGTTGGTAAACAAATGAGGGATGAACTCGGACAAA
GTGCTAACATCAAGAGTAAGCAAACAAGAAAGAATGTACAGTCAGCTATTGAAGTAATTATGCAAAGGATAAGATTATTT
AAAGCAGCTCCTGAAAATGGACTGGTTCTTTTTGTAGGTATGATTCCAAGAGGAGGTCCAGGAACTGAAAAAATGGAAAC
TTATGTTTTTGAACCTCCGGAACCTATTACAACTTACTGGTACCAGTGTAACAATGAGTTTTTCTTAGAACCTCTTGAGT
ATATGATTGAAGAAAGGGAAACTTATGGTTTGGCAGTTATTGACAGAAAAGAAGCTACAATAGCTACTTTAAGAGGTAAA
AAAGTAAACATTTTAAACCATTTGACAAGTGGTGTTCCGGGAAAACACAAAGCAGGAGGTCAATCACAAAGAAGGTTTGA
TCGTGTAATTGATTTGGCTGCTCATGAGTTTAAAAAACGTATCGGAGAACACATGAATGAAGATTTCTTAGCTTTGGAAG
AACTTGAAGGAGTTATTATTGGAGGTCCAGGTTTTACAAAAGAAGAATTTGTAAAAGGTGATTATCTTAACTACGAAATT
AAAGATAAAATCATAGCTACTGTAGATACTTCATATACTGGGGAATTTGGTATTAGGGAAGTAATAGACAAATCTGCAGA
TATTTTAAATGATTTGGATGTTATGCAGGAGAAAAAAGTAGTTCAAAAATTCCTCCATGAGTTAGTTAAAGATAAAGGGC
TTGCTTCTTATGGTGAAAGGGAAGTCAGGACTAACTTAATTATGGGTGCAGTTGACACATTATTATTATCTGAAGATTTA
ACTGCAATGCGTAAAGTTTTCAAATGTCCTAGCTGCGGCAATGAAGAGGAAATTACTGTCAAATCTCAAAGTGAAGCAGA
TAAACTTGAAAAACCATGTTCCAATTGTGGTGAAATTTTAAAAGAAGAATCTTCACAAACTTTAATTGAAGATTTTGTTG
AAAAAGCTGAAGAAATGAATTCTGAAGTTGAGCTTATTTCTACAGAAACTGAAGAAGGTATGCAACTCTTAAGAGCATTT
GGTGGAGTTGCAGCGATTTTAAGATACCATGTTGGATAA

Upstream 100 bases:

>100_bases
TTATTAATAGTTAGTATTTATAGTATTTAAAGTTCTTTCATTAATTATTTATAAGGTGAAATTACATAAATATATTATTA
TATTAATTTAAGGGATTAAC

Downstream 100 bases:

>100_bases
ATATTTTTAATTCCAATCATGGTTTTTATTTTGAATATTCCATACAAACATAGCTGTCTTCTTCATCAGTCATGGTGAAC
CTTCAGTTTATGGTTATGAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAEVSSKELYKFKKTLKELSEKKGRGTELVSVYIPHDKQISDVGKQMRDELGQSANIKSKQTRKNVQSAIEVIMQRIRLF
KAAPENGLVLFVGMIPRGGPGTEKMETYVFEPPEPITTYWYQCNNEFFLEPLEYMIEERETYGLAVIDRKEATIATLRGK
KVNILNHLTSGVPGKHKAGGQSQRRFDRVIDLAAHEFKKRIGEHMNEDFLALEELEGVIIGGPGFTKEEFVKGDYLNYEI
KDKIIATVDTSYTGEFGIREVIDKSADILNDLDVMQEKKVVQKFLHELVKDKGLASYGEREVRTNLIMGAVDTLLLSEDL
TAMRKVFKCPSCGNEEEITVKSQSEADKLEKPCSNCGEILKEESSQTLIEDFVEKAEEMNSEVELISTETEEGMQLLRAF
GGVAAILRYHVG

Sequences:

>Translated_412_residues
MAEVSSKELYKFKKTLKELSEKKGRGTELVSVYIPHDKQISDVGKQMRDELGQSANIKSKQTRKNVQSAIEVIMQRIRLF
KAAPENGLVLFVGMIPRGGPGTEKMETYVFEPPEPITTYWYQCNNEFFLEPLEYMIEERETYGLAVIDRKEATIATLRGK
KVNILNHLTSGVPGKHKAGGQSQRRFDRVIDLAAHEFKKRIGEHMNEDFLALEELEGVIIGGPGFTKEEFVKGDYLNYEI
KDKIIATVDTSYTGEFGIREVIDKSADILNDLDVMQEKKVVQKFLHELVKDKGLASYGEREVRTNLIMGAVDTLLLSEDL
TAMRKVFKCPSCGNEEEITVKSQSEADKLEKPCSNCGEILKEESSQTLIEDFVEKAEEMNSEVELISTETEEGMQLLRAF
GGVAAILRYHVG
>Mature_411_residues
AEVSSKELYKFKKTLKELSEKKGRGTELVSVYIPHDKQISDVGKQMRDELGQSANIKSKQTRKNVQSAIEVIMQRIRLFK
AAPENGLVLFVGMIPRGGPGTEKMETYVFEPPEPITTYWYQCNNEFFLEPLEYMIEERETYGLAVIDRKEATIATLRGKK
VNILNHLTSGVPGKHKAGGQSQRRFDRVIDLAAHEFKKRIGEHMNEDFLALEELEGVIIGGPGFTKEEFVKGDYLNYEIK
DKIIATVDTSYTGEFGIREVIDKSADILNDLDVMQEKKVVQKFLHELVKDKGLASYGEREVRTNLIMGAVDTLLLSEDLT
AMRKVFKCPSCGNEEEITVKSQSEADKLEKPCSNCGEILKEESSQTLIEDFVEKAEEMNSEVELISTETEEGMQLLRAFG
GVAAILRYHVG

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family

Homologues:

Organism=Homo sapiens, GI4759034, Length=406, Percent_Identity=32.7586206896552, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI72000368, Length=406, Percent_Identity=30.7881773399015, Blast_Score=224, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6319619, Length=406, Percent_Identity=31.7733990147783, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI28574632, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21356433, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24667392, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24667388, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24667380, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24667384, Length=409, Percent_Identity=33.0073349633252, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF1_METS3 (A5ULL8)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273464.1
- ProteinModelPortal:   A5ULL8
- SMR:   A5ULL8
- STRING:   A5ULL8
- GeneID:   5216024
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0891
- eggNOG:   arNOG04578
- HOGENOM:   HBG497188
- OMA:   GDYLHHE
- ProtClustDB:   PRK04011
- GO:   GO:0005737
- HAMAP:   MF_00424
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403
- PANTHER:   PTHR10113
- PANTHER:   PTHR10113:SF2
- TIGRFAMs:   TIGR03676

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 46510; Mature: 46379

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVSSKELYKFKKTLKELSEKKGRGTELVSVYIPHDKQISDVGKQMRDELGQSANIKSK
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHH
QTRKNVQSAIEVIMQRIRLFKAAPENGLVLFVGMIPRGGPGTEKMETYVFEPPEPITTYW
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHEEEECCCCCHHHHE
YQCNNEFFLEPLEYMIEERETYGLAVIDRKEATIATLRGKKVNILNHLTSGVPGKHKAGG
EECCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
QSQRRFDRVIDLAAHEFKKRIGEHMNEDFLALEELEGVIIGGPGFTKEEFVKGDYLNYEI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCCCHHHHHCCCCCCEEE
KDKIIATVDTSYTGEFGIREVIDKSADILNDLDVMQEKKVVQKFLHELVKDKGLASYGER
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHH
EVRTNLIMGAVDTLLLSEDLTAMRKVFKCPSCGNEEEITVKSQSEADKLEKPCSNCGEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHH
KEESSQTLIEDFVEKAEEMNSEVELISTETEEGMQLLRAFGGVAAILRYHVG
HCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEVSSKELYKFKKTLKELSEKKGRGTELVSVYIPHDKQISDVGKQMRDELGQSANIKSK
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHH
QTRKNVQSAIEVIMQRIRLFKAAPENGLVLFVGMIPRGGPGTEKMETYVFEPPEPITTYW
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHEEEECCCCCHHHHE
YQCNNEFFLEPLEYMIEERETYGLAVIDRKEATIATLRGKKVNILNHLTSGVPGKHKAGG
EECCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
QSQRRFDRVIDLAAHEFKKRIGEHMNEDFLALEELEGVIIGGPGFTKEEFVKGDYLNYEI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCCCHHHHHCCCCCCEEE
KDKIIATVDTSYTGEFGIREVIDKSADILNDLDVMQEKKVVQKFLHELVKDKGLASYGER
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHH
EVRTNLIMGAVDTLLLSEDLTAMRKVFKCPSCGNEEEITVKSQSEADKLEKPCSNCGEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHH
KEESSQTLIEDFVEKAEEMNSEVELISTETEEGMQLLRAFGGVAAILRYHVG
HCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA