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The map label for this gene is truA

Identifier: 148642915

GI number: 148642915

Start: 811480

End: 812352

Strand: Reverse

Name: truA

Synonym: Msm_0855

Alternate gene names: 148642915

Gene position: 812352-811480 (Counterclockwise)

Preceding gene: 148642916

Following gene: 148642913

Centisome position: 43.84

GC content: 31.96

Gene sequence:

>873_bases
ATGAAGAGGACAGCTTTAAAAATTGGCTATATCGGAACTAATTTTCATGGTTTTCAAAGACAACCTGATTTAAGAACTGT
TGAAGAAGAGCTGATTTACCATTTACGTAAACTAGGTTATATTGATGATTTAAAAAAGTCCAGATTTAGAATAGCCGGCA
GAACTGATGCAGGAGTTCATAGTTTAGGTAATGTAATCAGTTTCCAGTCTGAAAAAGAAGTTAGAGTTAATGAAATCAAT
AACAGTTTGCCTGATGATATTCAGATACTGGCTAAAGCTCCGGTAAGATTTGGTTTTAAACCGAGGTATGCTGAAATGAG
ACAGTATCGTTATGTTTTATTCAGGTCTGATTTGGATTTGTATAAACTGAATGAAGTAGCTGAAATTTTTAAAGGAACTC
ATAATTTTACTAATTTTACTAAACGATTCCAAAAAACAACAACCAGAACAATTGATGATATTAAAATCACCAAAGCAAAT
CTCAATGATTATCATAAAAAGGAATTTCCAAATTTGCATGACACACTTTCACCTGTTTTTGTAGATATTTATGGGGAAAG
CTTCTTATGGAACATGGTTCGTAAAATGATGCGTGTTTTTGTTGATGTAGCTATTGGGAAGTTATCTTTGGAAAAAGTTG
AAGAATTATTAAATCCTGCAGAGAATGACCCTAGAGCCAATATTAAAGTTTTGGATCCGGATTATCTGATTTTAATGGAT
ATTAAATATGATGGGGTTAAATTTGTTTATGATGATTATGCCTGTGAACGCTTTAAAAGAAATTTAGTTGATTCACTAGG
CGATTTGCAAAGAAAATATGCTATAAGGGAATCCATGATAAAAAGTTTGGAAGATCTGGAACGGATAAACTAA

Upstream 100 bases:

>100_bases
ATTAAATATGGTTGAATCATTAAAAGGTGTTGAATAACCTTTTACTTTTTTTTATTTTTGCAAAATTTATTTTAATTAAT
ATTTATAATAATATATTATT

Downstream 100 bases:

>100_bases
AAAACAGGTAATTTTTATTGTTACTTGAGTTTATTATGAAAAAATTCTGCATTAAAAATAAGTTTTGATAAAAAAGAAGA
ATTCGGGAAATAAGATGTCC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKRTALKIGYIGTNFHGFQRQPDLRTVEEELIYHLRKLGYIDDLKKSRFRIAGRTDAGVHSLGNVISFQSEKEVRVNEIN
NSLPDDIQILAKAPVRFGFKPRYAEMRQYRYVLFRSDLDLYKLNEVAEIFKGTHNFTNFTKRFQKTTTRTIDDIKITKAN
LNDYHKKEFPNLHDTLSPVFVDIYGESFLWNMVRKMMRVFVDVAIGKLSLEKVEELLNPAENDPRANIKVLDPDYLILMD
IKYDGVKFVYDDYACERFKRNLVDSLGDLQRKYAIRESMIKSLEDLERIN

Sequences:

>Translated_290_residues
MKRTALKIGYIGTNFHGFQRQPDLRTVEEELIYHLRKLGYIDDLKKSRFRIAGRTDAGVHSLGNVISFQSEKEVRVNEIN
NSLPDDIQILAKAPVRFGFKPRYAEMRQYRYVLFRSDLDLYKLNEVAEIFKGTHNFTNFTKRFQKTTTRTIDDIKITKAN
LNDYHKKEFPNLHDTLSPVFVDIYGESFLWNMVRKMMRVFVDVAIGKLSLEKVEELLNPAENDPRANIKVLDPDYLILMD
IKYDGVKFVYDDYACERFKRNLVDSLGDLQRKYAIRESMIKSLEDLERIN
>Mature_290_residues
MKRTALKIGYIGTNFHGFQRQPDLRTVEEELIYHLRKLGYIDDLKKSRFRIAGRTDAGVHSLGNVISFQSEKEVRVNEIN
NSLPDDIQILAKAPVRFGFKPRYAEMRQYRYVLFRSDLDLYKLNEVAEIFKGTHNFTNFTKRFQKTTTRTIDDIKITKAN
LNDYHKKEFPNLHDTLSPVFVDIYGESFLWNMVRKMMRVFVDVAIGKLSLEKVEELLNPAENDPRANIKVLDPDYLILMD
IKYDGVKFVYDDYACERFKRNLVDSLGDLQRKYAIRESMIKSLEDLERIN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=317, Percent_Identity=29.3375394321767, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI70166634, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70166599, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70166645, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788657, Length=261, Percent_Identity=26.4367816091954, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985046, Length=323, Percent_Identity=24.4582043343653, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17565126, Length=231, Percent_Identity=26.4069264069264, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321112, Length=286, Percent_Identity=28.6713286713287, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321375, Length=113, Percent_Identity=33.6283185840708, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325044, Length=121, Percent_Identity=34.7107438016529, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648455, Length=242, Percent_Identity=26.0330578512397, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281362146, Length=242, Percent_Identity=26.0330578512397, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_METS3 (A5ULI2)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273428.1
- ProteinModelPortal:   A5ULI2
- SMR:   A5ULI2
- STRING:   A5ULI2
- GeneID:   5216409
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0855
- eggNOG:   arNOG07659
- HOGENOM:   HBG621020
- OMA:   KSFLWEM
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 34125; Mature: 34125

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTALKIGYIGTNFHGFQRQPDLRTVEEELIYHLRKLGYIDDLKKSRFRIAGRTDAGVH
CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCHHHH
SLGNVISFQSEKEVRVNEINNSLPDDIQILAKAPVRFGFKPRYAEMRQYRYVLFRSDLDL
HHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCH
YKLNEVAEIFKGTHNFTNFTKRFQKTTTRTIDDIKITKANLNDYHKKEFPNLHDTLSPVF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHH
VDIYGESFLWNMVRKMMRVFVDVAIGKLSLEKVEELLNPAENDPRANIKVLDPDYLILMD
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEE
IKYDGVKFVYDDYACERFKRNLVDSLGDLQRKYAIRESMIKSLEDLERIN
EEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRTALKIGYIGTNFHGFQRQPDLRTVEEELIYHLRKLGYIDDLKKSRFRIAGRTDAGVH
CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCHHHH
SLGNVISFQSEKEVRVNEINNSLPDDIQILAKAPVRFGFKPRYAEMRQYRYVLFRSDLDL
HHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCH
YKLNEVAEIFKGTHNFTNFTKRFQKTTTRTIDDIKITKANLNDYHKKEFPNLHDTLSPVF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHH
VDIYGESFLWNMVRKMMRVFVDVAIGKLSLEKVEELLNPAENDPRANIKVLDPDYLILMD
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEE
IKYDGVKFVYDDYACERFKRNLVDSLGDLQRKYAIRESMIKSLEDLERIN
EEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA