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The map label for this gene is dphB [H]

Identifier: 148642861

GI number: 148642861

Start: 761906

End: 762718

Strand: Reverse

Name: dphB [H]

Synonym: Msm_0801

Alternate gene names: 148642861

Gene position: 762718-761906 (Counterclockwise)

Preceding gene: 148642863

Following gene: 148642860

Centisome position: 41.16

GC content: 32.96

Gene sequence:

>813_bases
ATGTTTTTAAAATCTTATACTATGTTTTATTTAGTTGGATTAGGATTATTTGATGCAGGCGATATCAGTCTTAAAGGTTT
GAAATGTTTAAAAAGTGTCGATAAAATTTATGCGGAATTTTTCACTTCCAGATTATTTGGTTCTAGCTTTGATGAGATTG
AATCTCAGATAGGTAAAAAAATTGAAGTTTTAGTTAGAAATGAAGTTGAAGAAGAAAGTAAATTTTTAGATGAAGCTATT
GATTTGGATGTAGCTTTAATTACTGGAGGGGATCCTTTAATAGCTACTACCCACAGTGATTTTTTAGTTCAATGTTCTAA
AAAAGGCATTGATTACGAAGTGATTCACGGATCATCTATTTTATCTTCTGCACCGGCTATTTCCGGACTTCAAGGCTATA
AGTTCGGTAAGGTTACAACCATTCCTTTCCCTGATCATAATTTCTATCCAAAATCTCCATATACAGCTATTGAAGAGAAC
TTGGCTATGGAGTTACATACTTTAGTTTTACTTGATATTCAAGCTCATAAAGACCGTTACATGACTGTTAATCAGGGTTT
GGAATATCTGATGAATATTAAAAATACTTTAGATTATGATGGGGCAGTTACTGAAGATACTCTTGCTGTTGGTATTGCAA
GGGTAGGCTCTAAAGATGTTGTTGTAAAAGCCGGTAAAATTAGTCAGTTGATTGATTATGATTTTGGAGGACCTCTTCAC
TGTATTGTTATTCCTTCCAAACTTCATATTGTTGAAGCAGAATATCTGGTTGAGATAGCTGGAGCTGACAGTGAAATTCT
TGATGAAATTTAA

Upstream 100 bases:

>100_bases
TCTTTTAATGGAACTTTAACTGATTTCATACTAAATACCTCTAATACTAATCTTTGTTTTAGTTTTATTATAATATTTTC
TAAAAAAACACTTTTATACT

Downstream 100 bases:

>100_bases
ATTAAAATAAGGGGAGAGAAGTAGAACGTGTTTTTTGGATTTTCTACTTCTTAAATTCAATGAAACATATCCTAATATAT
TTTTGGAGATTATAAAATGG

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MFLKSYTMFYLVGLGLFDAGDISLKGLKCLKSVDKIYAEFFTSRLFGSSFDEIESQIGKKIEVLVRNEVEEESKFLDEAI
DLDVALITGGDPLIATTHSDFLVQCSKKGIDYEVIHGSSILSSAPAISGLQGYKFGKVTTIPFPDHNFYPKSPYTAIEEN
LAMELHTLVLLDIQAHKDRYMTVNQGLEYLMNIKNTLDYDGAVTEDTLAVGIARVGSKDVVVKAGKISQLIDYDFGGPLH
CIVIPSKLHIVEAEYLVEIAGADSEILDEI

Sequences:

>Translated_270_residues
MFLKSYTMFYLVGLGLFDAGDISLKGLKCLKSVDKIYAEFFTSRLFGSSFDEIESQIGKKIEVLVRNEVEEESKFLDEAI
DLDVALITGGDPLIATTHSDFLVQCSKKGIDYEVIHGSSILSSAPAISGLQGYKFGKVTTIPFPDHNFYPKSPYTAIEEN
LAMELHTLVLLDIQAHKDRYMTVNQGLEYLMNIKNTLDYDGAVTEDTLAVGIARVGSKDVVVKAGKISQLIDYDFGGPLH
CIVIPSKLHIVEAEYLVEIAGADSEILDEI
>Mature_270_residues
MFLKSYTMFYLVGLGLFDAGDISLKGLKCLKSVDKIYAEFFTSRLFGSSFDEIESQIGKKIEVLVRNEVEEESKFLDEAI
DLDVALITGGDPLIATTHSDFLVQCSKKGIDYEVIHGSSILSSAPAISGLQGYKFGKVTTIPFPDHNFYPKSPYTAIEEN
LAMELHTLVLLDIQAHKDRYMTVNQGLEYLMNIKNTLDYDGAVTEDTLAVGIARVGSKDVVVKAGKISQLIDYDFGGPLH
CIVIPSKLHIVEAEYLVEIAGADSEILDEI

Specific function: Required for the methylation step in diphthamide biosynthesis [H]

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family [H]

Homologues:

Organism=Homo sapiens, GI117190326, Length=269, Percent_Identity=39.4052044609665, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI117190262, Length=269, Percent_Identity=39.4052044609665, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI117190324, Length=268, Percent_Identity=39.1791044776119, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17531369, Length=269, Percent_Identity=33.457249070632, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323201, Length=253, Percent_Identity=39.9209486166008, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572120, Length=273, Percent_Identity=37.3626373626374, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.98 [H]

Molecular weight: Translated: 29773; Mature: 29773

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKSYTMFYLVGLGLFDAGDISLKGLKCLKSVDKIYAEFFTSRLFGSSFDEIESQIGKK
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
IEVLVRNEVEEESKFLDEAIDLDVALITGGDPLIATTHSDFLVQCSKKGIDYEVIHGSSI
EEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCHHHEEECCCCCCEEEEECCHH
LSSAPAISGLQGYKFGKVTTIPFPDHNFYPKSPYTAIEENLAMELHTLVLLDIQAHKDRY
HHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCE
MTVNQGLEYLMNIKNTLDYDGAVTEDTLAVGIARVGSKDVVVKAGKISQLIDYDFGGPLH
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEE
CIVIPSKLHIVEAEYLVEIAGADSEILDEI
EEEECCEEEEEEHHHEEEECCCCHHHHHCC
>Mature Secondary Structure
MFLKSYTMFYLVGLGLFDAGDISLKGLKCLKSVDKIYAEFFTSRLFGSSFDEIESQIGKK
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
IEVLVRNEVEEESKFLDEAIDLDVALITGGDPLIATTHSDFLVQCSKKGIDYEVIHGSSI
EEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCHHHEEECCCCCCEEEEECCHH
LSSAPAISGLQGYKFGKVTTIPFPDHNFYPKSPYTAIEENLAMELHTLVLLDIQAHKDRY
HHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCE
MTVNQGLEYLMNIKNTLDYDGAVTEDTLAVGIARVGSKDVVVKAGKISQLIDYDFGGPLH
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEE
CIVIPSKLHIVEAEYLVEIAGADSEILDEI
EEEECCEEEEEEHHHEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]