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The map label for this gene is topA [H]

Identifier: 148642777

GI number: 148642777

Start: 686051

End: 688201

Strand: Reverse

Name: topA [H]

Synonym: Msm_0717

Alternate gene names: 148642777

Gene position: 688201-686051 (Counterclockwise)

Preceding gene: 148642778

Following gene: 148642776

Centisome position: 37.14

GC content: 34.77

Gene sequence:

>2151_bases
ATGAATGAAGTAATAATTTGCGAAAAACCAAAATCTGCAGAAAAAATAGCTAAAGCATTGTCTTCTAAAGCTAAAAAGAA
CGTATTTAATAAAAAAGTTAAATATTGGACATTGGAAAGAGACGGTAAGGATATAACAGTATTGTCTGCTGTCGGACACT
TATATTCTTTAACTCCGGATAATCCCAAGGATAAAGTCTATTTTGATTTGCATTGGGCACCATTATTTGAGATTGACAAA
AAATATAAGAACTATACCAAGGATTATGTAAGGGCTATTAAAAAATATGCTAAAGATGCTGACAGCTACATTCATGCATG
CGATTATGATATTGAAGGAACTTTAATCGGATATCATGCATTAAAATATGGGTGTGGCGATGATGCTTTAAAAAAATCTT
CAAGAATGAAGTTTTCCACTTTAACTAAAAAGGATATTGTGGAAGCATATGAAAATAGAATACCTATTGACAGACATCAG
GTAGACAGCGGTGTAGCCAGACATATTCTGGACTTTTATTTTGGTGTAAACATATCAAAGGCTTTAATGAAATCTGTAAG
CGCAGCCAAAAAAAGATTTTTAAAGTTATCTGCAGGAAGAGTTCAAACTCCGACTTTGTCTATTTTGGTTGACCGTGAAA
AAGAGATTAAAAAATTTGTACCTAAACCTTACTGGTTAATTAAAGCTTTACTTGACGGTGAAATTGAAGCGGATCATGTA
GATGGAAAAATATTCGATAAAACAAGGGCTGAAGAGATTTTTTCAAACTGTGAAAATAGTGATGCAGCTGTCGATAAAAT
CAAATATTCCAATTCCACTACAAAACCTCCTGTTCCATTTAATTTAGGTGGTTTGCAGTCTGAAGCTTATAATGTCTTTG
GTTTTTCACCTAAAAAAACTCAGACTATTGCTCAAAGCCTTTATACTGCAGGATATACATCTTATCCACGTACTTCATCC
CAAAAGTTGCCTGAAAGCCTGGATTTCAAAAGCATATTTTTACAGCTGTCCAAAAATTCCGAATTCAGAACACATATTTC
AGCTCTTCCGGCTAAATTAAAACCTAATGAAGGTAAAAAGGAAGATGCTGCTCACCCTGCTATTCATCCGACAGGTATTT
TACCTGATAAATTGGACAAGGATGAGTTAAAAATTTATGAACTGATTGTTTACAGGTTTATTTCAGTATTTTTCGAAGCA
GCTACCTTTGAAACAATGAGTACCACTTTGGACATTGGCGGTGAAAAATTCAAGTTTAGACGCAGAAGGGTTACCCATAA
AGGATGGCTGGAACACTATCCATTTAAGAAAATAGATAATGAGGAATTTCCTGATGTTAAGGAAGGAGATTCCATCATCG
TCAAGGAGATTGTATCAGAAGAAAAAGAAACCAAACCTCCTGCAAGATATAATCAGGCTTCTTTAATTAAGGAACTTGAA
AAAAGAGAACTTGGAACCAAAGCTACCCGTGCAGATATTATTGATAAGTTATATGACAGAAAATATATAACTGGAAACAA
AATTGAGGTTAATCAGCTTGGTGAGAACATAATCGACACTTTAAGCGAGTACTGTTCTAACTTAACAAGTGAAGAGCTGA
CCCGTGACTTTGAAAATAAACTTGAAGGCATAGACAATGATAAAGCCACCCGTGAAAGTGTTGTAGCTGAAGGTGAAAAG
GAAGTTAAGGTTATTTTGGGAGATATTGATAAAAACAAAGTTAAAATCGGTTCACAGATTTATGATGCCTATCAGGAAAG
CAATATTGTTGGAAAATGTAAATGCGGCGGAAATCTGGTTAAAAAATACTCTCCTAAAAATAAAAGCACTTTTGTGGGAT
GTTCTAATTATCCTGACTGTAAAGCAACTTACTCTGTTCCTAAAGGAGCAAATTTCTTAAAGAAAACCTGTAAAACATGT
GGATTACCTATTATTTCCTTTGGTAAGCCACGTCAGCATGCATGTCTTGACCCTAACTGTGGTAAAGAGAATACTAAGCC
TCGTGAGATGAAAGTAGTTGGTGTGTGTCCTAGTTGTGGTAAAGAACTGATTAAAAGGTCCGGAAGGTATGGAGAATTTG
TTGGTTGCAGAGGATTTCCAAAATGTCGCTTTACCTGTTCTTTAGATGAGTTAAAAGATAAAGTCCAATAG

Upstream 100 bases:

>100_bases
CTTTCAATAGAAATATTTTTATAGGATTAATTATTTTAAATATTAAATCAGTAATTTACAAATTTTTATAATTTAAAAAT
TTAAAAACGGTACAGGTACA

Downstream 100 bases:

>100_bases
ACTTTATTTTTTCTAATTTTTTTTTAAATGCATAATCTTTAATAATGTTAAAAATAATAGATATAAATTAATAAAAGTAT
TGATGGTTTTGCCCAAAAAT

Product: DNA topoisomerase I, TopA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MNEVIICEKPKSAEKIAKALSSKAKKNVFNKKVKYWTLERDGKDITVLSAVGHLYSLTPDNPKDKVYFDLHWAPLFEIDK
KYKNYTKDYVRAIKKYAKDADSYIHACDYDIEGTLIGYHALKYGCGDDALKKSSRMKFSTLTKKDIVEAYENRIPIDRHQ
VDSGVARHILDFYFGVNISKALMKSVSAAKKRFLKLSAGRVQTPTLSILVDREKEIKKFVPKPYWLIKALLDGEIEADHV
DGKIFDKTRAEEIFSNCENSDAAVDKIKYSNSTTKPPVPFNLGGLQSEAYNVFGFSPKKTQTIAQSLYTAGYTSYPRTSS
QKLPESLDFKSIFLQLSKNSEFRTHISALPAKLKPNEGKKEDAAHPAIHPTGILPDKLDKDELKIYELIVYRFISVFFEA
ATFETMSTTLDIGGEKFKFRRRRVTHKGWLEHYPFKKIDNEEFPDVKEGDSIIVKEIVSEEKETKPPARYNQASLIKELE
KRELGTKATRADIIDKLYDRKYITGNKIEVNQLGENIIDTLSEYCSNLTSEELTRDFENKLEGIDNDKATRESVVAEGEK
EVKVILGDIDKNKVKIGSQIYDAYQESNIVGKCKCGGNLVKKYSPKNKSTFVGCSNYPDCKATYSVPKGANFLKKTCKTC
GLPIISFGKPRQHACLDPNCGKENTKPREMKVVGVCPSCGKELIKRSGRYGEFVGCRGFPKCRFTCSLDELKDKVQ

Sequences:

>Translated_716_residues
MNEVIICEKPKSAEKIAKALSSKAKKNVFNKKVKYWTLERDGKDITVLSAVGHLYSLTPDNPKDKVYFDLHWAPLFEIDK
KYKNYTKDYVRAIKKYAKDADSYIHACDYDIEGTLIGYHALKYGCGDDALKKSSRMKFSTLTKKDIVEAYENRIPIDRHQ
VDSGVARHILDFYFGVNISKALMKSVSAAKKRFLKLSAGRVQTPTLSILVDREKEIKKFVPKPYWLIKALLDGEIEADHV
DGKIFDKTRAEEIFSNCENSDAAVDKIKYSNSTTKPPVPFNLGGLQSEAYNVFGFSPKKTQTIAQSLYTAGYTSYPRTSS
QKLPESLDFKSIFLQLSKNSEFRTHISALPAKLKPNEGKKEDAAHPAIHPTGILPDKLDKDELKIYELIVYRFISVFFEA
ATFETMSTTLDIGGEKFKFRRRRVTHKGWLEHYPFKKIDNEEFPDVKEGDSIIVKEIVSEEKETKPPARYNQASLIKELE
KRELGTKATRADIIDKLYDRKYITGNKIEVNQLGENIIDTLSEYCSNLTSEELTRDFENKLEGIDNDKATRESVVAEGEK
EVKVILGDIDKNKVKIGSQIYDAYQESNIVGKCKCGGNLVKKYSPKNKSTFVGCSNYPDCKATYSVPKGANFLKKTCKTC
GLPIISFGKPRQHACLDPNCGKENTKPREMKVVGVCPSCGKELIKRSGRYGEFVGCRGFPKCRFTCSLDELKDKVQ
>Mature_716_residues
MNEVIICEKPKSAEKIAKALSSKAKKNVFNKKVKYWTLERDGKDITVLSAVGHLYSLTPDNPKDKVYFDLHWAPLFEIDK
KYKNYTKDYVRAIKKYAKDADSYIHACDYDIEGTLIGYHALKYGCGDDALKKSSRMKFSTLTKKDIVEAYENRIPIDRHQ
VDSGVARHILDFYFGVNISKALMKSVSAAKKRFLKLSAGRVQTPTLSILVDREKEIKKFVPKPYWLIKALLDGEIEADHV
DGKIFDKTRAEEIFSNCENSDAAVDKIKYSNSTTKPPVPFNLGGLQSEAYNVFGFSPKKTQTIAQSLYTAGYTSYPRTSS
QKLPESLDFKSIFLQLSKNSEFRTHISALPAKLKPNEGKKEDAAHPAIHPTGILPDKLDKDELKIYELIVYRFISVFFEA
ATFETMSTTLDIGGEKFKFRRRRVTHKGWLEHYPFKKIDNEEFPDVKEGDSIIVKEIVSEEKETKPPARYNQASLIKELE
KRELGTKATRADIIDKLYDRKYITGNKIEVNQLGENIIDTLSEYCSNLTSEELTRDFENKLEGIDNDKATRESVVAEGEK
EVKVILGDIDKNKVKIGSQIYDAYQESNIVGKCKCGGNLVKKYSPKNKSTFVGCSNYPDCKATYSVPKGANFLKKTCKTC
GLPIISFGKPRQHACLDPNCGKENTKPREMKVVGVCPSCGKELIKRSGRYGEFVGCRGFPKCRFTCSLDELKDKVQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=580, Percent_Identity=24.3103448275862, Blast_Score=166, Evalue=9e-41,
Organism=Homo sapiens, GI4507635, Length=584, Percent_Identity=26.1986301369863, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1787529, Length=658, Percent_Identity=29.3313069908815, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1788061, Length=610, Percent_Identity=26.0655737704918, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17555378, Length=617, Percent_Identity=25.7698541329011, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32563869, Length=765, Percent_Identity=25.6209150326797, Blast_Score=153, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323263, Length=520, Percent_Identity=25.3846153846154, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24585251, Length=689, Percent_Identity=25.544267053701, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24640096, Length=660, Percent_Identity=23.4848484848485, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81067; Mature: 81067

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVIICEKPKSAEKIAKALSSKAKKNVFNKKVKYWTLERDGKDITVLSAVGHLYSLTPD
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHEECCCC
NPKDKVYFDLHWAPLFEIDKKYKNYTKDYVRAIKKYAKDADSYIHACDYDIEGTLIGYHA
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
LKYGCGDDALKKSSRMKFSTLTKKDIVEAYENRIPIDRHQVDSGVARHILDFYFGVNISK
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
ALMKSVSAAKKRFLKLSAGRVQTPTLSILVDREKEIKKFVPKPYWLIKALLDGEIEADHV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DGKIFDKTRAEEIFSNCENSDAAVDKIKYSNSTTKPPVPFNLGGLQSEAYNVFGFSPKKT
CCCHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
QTIAQSLYTAGYTSYPRTSSQKLPESLDFKSIFLQLSKNSEFRTHISALPAKLKPNEGKK
HHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
EDAAHPAIHPTGILPDKLDKDELKIYELIVYRFISVFFEAATFETMSTTLDIGGEKFKFR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RRRVTHKGWLEHYPFKKIDNEEFPDVKEGDSIIVKEIVSEEKETKPPARYNQASLIKELE
HHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KRELGTKATRADIIDKLYDRKYITGNKIEVNQLGENIIDTLSEYCSNLTSEELTRDFENK
HHHCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LEGIDNDKATRESVVAEGEKEVKVILGDIDKNKVKIGSQIYDAYQESNIVGKCKCGGNLV
HCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHH
KKYSPKNKSTFVGCSNYPDCKATYSVPKGANFLKKTCKTCGLPIISFGKPRQHACLDPNC
HHCCCCCCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
GKENTKPREMKVVGVCPSCGKELIKRSGRYGEFVGCRGFPKCRFTCSLDELKDKVQ
CCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHCC
>Mature Secondary Structure
MNEVIICEKPKSAEKIAKALSSKAKKNVFNKKVKYWTLERDGKDITVLSAVGHLYSLTPD
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHEECCCC
NPKDKVYFDLHWAPLFEIDKKYKNYTKDYVRAIKKYAKDADSYIHACDYDIEGTLIGYHA
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
LKYGCGDDALKKSSRMKFSTLTKKDIVEAYENRIPIDRHQVDSGVARHILDFYFGVNISK
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
ALMKSVSAAKKRFLKLSAGRVQTPTLSILVDREKEIKKFVPKPYWLIKALLDGEIEADHV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DGKIFDKTRAEEIFSNCENSDAAVDKIKYSNSTTKPPVPFNLGGLQSEAYNVFGFSPKKT
CCCHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
QTIAQSLYTAGYTSYPRTSSQKLPESLDFKSIFLQLSKNSEFRTHISALPAKLKPNEGKK
HHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
EDAAHPAIHPTGILPDKLDKDELKIYELIVYRFISVFFEAATFETMSTTLDIGGEKFKFR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RRRVTHKGWLEHYPFKKIDNEEFPDVKEGDSIIVKEIVSEEKETKPPARYNQASLIKELE
HHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KRELGTKATRADIIDKLYDRKYITGNKIEVNQLGENIIDTLSEYCSNLTSEELTRDFENK
HHHCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LEGIDNDKATRESVVAEGEKEVKVILGDIDKNKVKIGSQIYDAYQESNIVGKCKCGGNLV
HCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHH
KKYSPKNKSTFVGCSNYPDCKATYSVPKGANFLKKTCKTCGLPIISFGKPRQHACLDPNC
HHCCCCCCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
GKENTKPREMKVVGVCPSCGKELIKRSGRYGEFVGCRGFPKCRFTCSLDELKDKVQ
CCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]