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The map label for this gene is ilvB [H]

Identifier: 148642746

GI number: 148642746

Start: 658642

End: 660180

Strand: Reverse

Name: ilvB [H]

Synonym: Msm_0686

Alternate gene names: 148642746

Gene position: 660180-658642 (Counterclockwise)

Preceding gene: 148642747

Following gene: 148642740

Centisome position: 35.62

GC content: 36.65

Gene sequence:

>1539_bases
ATGAATGTAGCTGCTTATTTAGTTAAAATCCTTGAAGAGGAAGGTCTGGAAAATATTTTCGGACTTCCGGGAGAACAGAT
TCTGCCGTTTTATAAGGCTCTTAAAGACTCCAAAATAAATCATATTCTGGTAAGACACGAACAGGCAGCTATGCATGCGG
CAGATGCATATTACAAATCATCCCATAAATTAAGTGCATGTGTAGCTACTGCAGCACCGGGAGCACTTAATTTTACAATG
GCTCTGGCAGGAGCATATAAGGACAATGTGCCTCTTCTGGTTTTGACAGGAGACAATCCCACTGATTTGAGAAATGAGGA
TGTTTTCCAGTCACTTCCGACATCTGAAATTTTTAGAAATATTGTTGACGAAACATTCAATCCCTTAAACGGAACTGAAG
CGGTATATGCACTAAGGGCAGCTATTTATAAAATTAGGCACGACCCAAAAGGCCCGGTTCATATAAACTTGTCAAATGAT
GTCCTGTTAAGCGAGGAGTTTCAGGACTTTGATTTATGTTATTTGTGTGAAAATGACCTGTCAAATGTTAAAAAAGCCCA
GGAATTAATAAACAAATCTAAAAAACCGCTCTTTGTATTGGGTTCTGGAGCAATATCACAAAGAAAAGTTTTAAAACTGA
TAGCTGAAAGTAATGGAATTCCGGTAGCAACTACTTTTAATTCAAAAGGAATCATAGCTGAAAGCAATCCTTTAAATCTG
GGAATGGCAGGTACCAGAGGAACTCCCAGAGCAAATTATGCCATAAAAAATTCAGACTGTATCATAGCTTTAGGTGCAAG
AGCTTCTGAAAGGACATTTGTTGCTTTGGATGAAATTGAAGATAAGCTGATTCATGTAAATATTAATAAAAAGCATCTTA
AAGGAAATTATCCGATTCAGGGAAGTGTTGAAAACTTCTTATATGAAGTAGACTTTAAAAAAGCAGACTGGCTAGATGAA
CTGTTGGAAATTGACAACAAAATAGCTGTTGAAGGTTTGGGGGATAAAACACTTCCGTTAAGACCTCAGGTGGCTATTAA
TGAAATTCTAAATCAGTATAAGGACAATATTATAATCGGTGATGCCGGAAGTCATATTACATGGGTAACTCTGCTTAAAA
AATCCTCTGACTTTGGCCAGCTGCTGTTTCAGTCAGCACTTGGACCAATGGGTTACGGAATTCCGGGAGCTATCGGAGCT
GCAGTTGCAAATCCTGATAAAAAAATAATTGTAATCAACGGAGACGGAGATTTCCAGATGAATATTCAGGAACTGGCTAC
AATAAAAGAATATGGCTTGAATATTTTAATTTTTATAATAAACAACTCACAATACGGCATTATAAGGCAGCATGAAGTTA
ATAAATATGATATGGAACCCTATCAGATAGATTTAAAAAATCCTGACTTTGTAAAAGTAGCTGAAGCATACGGATTAAAA
GCAAAAAGGGCAGAAAAACTTGAAGATCTCCAGGATGTGGATGATTATGATATTGTAGATGTTGCTGTAAGGTTTGAAGA
TATTCCCCTTCCTAAATAA

Upstream 100 bases:

>100_bases
AATACAAAATCAAAAAAGGGGACAGAATAGCTCAGGGAGTTGTTTTTGAAGTTGAAGGCTCCGGGCAATACAGCGGATCT
TATCAGGAAGAGGAATAATA

Downstream 100 bases:

>100_bases
GGCACTCTTCAACAAATTTTTTTACAATATCTTCATATCCCAGGCAGTTGCCGTCAAATGAATAGTAATAGTATTCCTCA
TCCTGGTTATGGATAAATCC

Product: acetolactate synthase (TPP-requiring), large subunit, IlvB

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MNVAAYLVKILEEEGLENIFGLPGEQILPFYKALKDSKINHILVRHEQAAMHAADAYYKSSHKLSACVATAAPGALNFTM
ALAGAYKDNVPLLVLTGDNPTDLRNEDVFQSLPTSEIFRNIVDETFNPLNGTEAVYALRAAIYKIRHDPKGPVHINLSND
VLLSEEFQDFDLCYLCENDLSNVKKAQELINKSKKPLFVLGSGAISQRKVLKLIAESNGIPVATTFNSKGIIAESNPLNL
GMAGTRGTPRANYAIKNSDCIIALGARASERTFVALDEIEDKLIHVNINKKHLKGNYPIQGSVENFLYEVDFKKADWLDE
LLEIDNKIAVEGLGDKTLPLRPQVAINEILNQYKDNIIIGDAGSHITWVTLLKKSSDFGQLLFQSALGPMGYGIPGAIGA
AVANPDKKIIVINGDGDFQMNIQELATIKEYGLNILIFIINNSQYGIIRQHEVNKYDMEPYQIDLKNPDFVKVAEAYGLK
AKRAEKLEDLQDVDDYDIVDVAVRFEDIPLPK

Sequences:

>Translated_512_residues
MNVAAYLVKILEEEGLENIFGLPGEQILPFYKALKDSKINHILVRHEQAAMHAADAYYKSSHKLSACVATAAPGALNFTM
ALAGAYKDNVPLLVLTGDNPTDLRNEDVFQSLPTSEIFRNIVDETFNPLNGTEAVYALRAAIYKIRHDPKGPVHINLSND
VLLSEEFQDFDLCYLCENDLSNVKKAQELINKSKKPLFVLGSGAISQRKVLKLIAESNGIPVATTFNSKGIIAESNPLNL
GMAGTRGTPRANYAIKNSDCIIALGARASERTFVALDEIEDKLIHVNINKKHLKGNYPIQGSVENFLYEVDFKKADWLDE
LLEIDNKIAVEGLGDKTLPLRPQVAINEILNQYKDNIIIGDAGSHITWVTLLKKSSDFGQLLFQSALGPMGYGIPGAIGA
AVANPDKKIIVINGDGDFQMNIQELATIKEYGLNILIFIINNSQYGIIRQHEVNKYDMEPYQIDLKNPDFVKVAEAYGLK
AKRAEKLEDLQDVDDYDIVDVAVRFEDIPLPK
>Mature_512_residues
MNVAAYLVKILEEEGLENIFGLPGEQILPFYKALKDSKINHILVRHEQAAMHAADAYYKSSHKLSACVATAAPGALNFTM
ALAGAYKDNVPLLVLTGDNPTDLRNEDVFQSLPTSEIFRNIVDETFNPLNGTEAVYALRAAIYKIRHDPKGPVHINLSND
VLLSEEFQDFDLCYLCENDLSNVKKAQELINKSKKPLFVLGSGAISQRKVLKLIAESNGIPVATTFNSKGIIAESNPLNL
GMAGTRGTPRANYAIKNSDCIIALGARASERTFVALDEIEDKLIHVNINKKHLKGNYPIQGSVENFLYEVDFKKADWLDE
LLEIDNKIAVEGLGDKTLPLRPQVAINEILNQYKDNIIIGDAGSHITWVTLLKKSSDFGQLLFQSALGPMGYGIPGAIGA
AVANPDKKIIVINGDGDFQMNIQELATIKEYGLNILIFIINNSQYGIIRQHEVNKYDMEPYQIDLKNPDFVKVAEAYGLK
AKRAEKLEDLQDVDDYDIVDVAVRFEDIPLPK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=516, Percent_Identity=24.2248062015504, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=22.7848101265823, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1790104, Length=496, Percent_Identity=30.6451612903226, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=29.0676416819013, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786717, Length=523, Percent_Identity=27.7246653919694, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=25.0922509225092, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1788716, Length=485, Percent_Identity=24.5360824742268, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17531299, Length=484, Percent_Identity=25, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=484, Percent_Identity=25, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=22.4677716390424, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=530, Percent_Identity=28.4905660377358, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320123, Length=505, Percent_Identity=23.7623762376238, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=22.9508196721311, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=546, Percent_Identity=23.0769230769231, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56672; Mature: 56672

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAAYLVKILEEEGLENIFGLPGEQILPFYKALKDSKINHILVRHEQAAMHAADAYYKS
CCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHC
SHKLSACVATAAPGALNFTMALAGAYKDNVPLLVLTGDNPTDLRNEDVFQSLPTSEIFRN
CCCHHEEHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHH
IVDETFNPLNGTEAVYALRAAIYKIRHDPKGPVHINLSNDVLLSEEFQDFDLCYLCENDL
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCEEEEECHHH
SNVKKAQELINKSKKPLFVLGSGAISQRKVLKLIAESNGIPVATTFNSKGIIAESNPLNL
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCEE
GMAGTRGTPRANYAIKNSDCIIALGARASERTFVALDEIEDKLIHVNINKKHLKGNYPIQ
EECCCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCEEEEEECCHHHCCCCCCCC
GSVENFLYEVDFKKADWLDELLEIDNKIAVEGLGDKTLPLRPQVAINEILNQYKDNIIIG
CCHHHEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEE
DAGSHITWVTLLKKSSDFGQLLFQSALGPMGYGIPGAIGAAVANPDKKIIVINGDGDFQM
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEE
NIQELATIKEYGLNILIFIINNSQYGIIRQHEVNKYDMEPYQIDLKNPDFVKVAEAYGLK
CHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEECCCCCEEEHHHHHCCC
AKRAEKLEDLQDVDDYDIVDVAVRFEDIPLPK
HHHHHHHHHHHCCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MNVAAYLVKILEEEGLENIFGLPGEQILPFYKALKDSKINHILVRHEQAAMHAADAYYKS
CCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHC
SHKLSACVATAAPGALNFTMALAGAYKDNVPLLVLTGDNPTDLRNEDVFQSLPTSEIFRN
CCCHHEEHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHH
IVDETFNPLNGTEAVYALRAAIYKIRHDPKGPVHINLSNDVLLSEEFQDFDLCYLCENDL
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCEEEEECHHH
SNVKKAQELINKSKKPLFVLGSGAISQRKVLKLIAESNGIPVATTFNSKGIIAESNPLNL
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCEE
GMAGTRGTPRANYAIKNSDCIIALGARASERTFVALDEIEDKLIHVNINKKHLKGNYPIQ
EECCCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCEEEEEECCHHHCCCCCCCC
GSVENFLYEVDFKKADWLDELLEIDNKIAVEGLGDKTLPLRPQVAINEILNQYKDNIIIG
CCHHHEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEE
DAGSHITWVTLLKKSSDFGQLLFQSALGPMGYGIPGAIGAAVANPDKKIIVINGDGDFQM
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEE
NIQELATIKEYGLNILIFIINNSQYGIIRQHEVNKYDMEPYQIDLKNPDFVKVAEAYGLK
CHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEECCCCCEEEHHHHHCCC
AKRAEKLEDLQDVDDYDIVDVAVRFEDIPLPK
HHHHHHHHHHHCCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]