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The map label for this gene is carA [H]

Identifier: 148642549

GI number: 148642549

Start: 463786

End: 464868

Strand: Reverse

Name: carA [H]

Synonym: Msm_0489

Alternate gene names: 148642549

Gene position: 464868-463786 (Counterclockwise)

Preceding gene: 148642550

Following gene: 148642548

Centisome position: 25.09

GC content: 33.52

Gene sequence:

>1083_bases
ATGGTAAAAATGGCTAAATTAGCTTTGGAAGATGGTACAGTTTTAAAAGGTGATGGTTTCGGTTATGAAACTACTAAGGT
AGGGGAACTTGTTTTTTCAACTGGTATGGTTGGTTATACTGAATCTTTAACTGATCCTTCTTTTAAAGGAGAAATATTAA
TGTCTACTTATCCTTTAGAGGGAAATTATGGGGTAAGTGAAGAATGGTATCAGTCAGATAAAATTCAAGCTGAAGGTTTT
GTTGTAAGGGAAGTTTGCAGAGAAGTTTCTAATTTTTCATCACAAAAAACTTTAGATGATTTTTTAAATGAATTTGAAAC
TCCTGGAATTAGTGGAATCGACACACGTGATTTAACTCTTAAAATTCGTGAAGAAGGTTCAATGAAAGCAGCTATTTCAA
CTGAAGACATTCCTGACAGTGAATTAATAGAACTTGCACGTGCTCAACAAAGCATTATTGATATTGACGTTGTGCCGCAG
GTATCTACTAAAGAAATTAAAGTTTTCAATGAGGATGCCAACAAAAAAGTAGCTTTAATTGACTGCGGTGTTAAGAAAAA
TATTATAAACAACTTTTTGGAACGTGGAATAGGAGTTGTTTTGTTCCCATATGATACTGATTATAAAACTATTTTGAATT
ACTCTCCTGACGGTTTGATGATTACTTCAGGACCAGGAAATCCTGATAGGGTACATGAAACTATCAGCACAGTTCAAAAA
TTATCTGAAAGATTACCTATTTTTGGAATTTGTATGGGTCAGCAGTTAGTAGCTAAATCTTTTGGAGCCAGATCTTACAA
AATGAAATTTGGACACAGAGGGGCAAATCAGCCGGTAAAAGATTTGAAGACAGGTAAAGTATATATCACTTCTCAAAATC
ACGGATTTACTATTGATAAAGAGTCTATTAATGAGACTGACTTAGTTTTAACTCAAGTAAATCTTAATGATGGAACTCCT
GAAGGTTTTTCACATAAGGAATTGCCTGTTCACTGTATTCAGTACCATCCTGAAGCAGGACCTGGTCCAAATGATACAAG
ATATGTTTTTGATAAATTTAGTCAAATGATGGATGAATATTAA

Upstream 100 bases:

>100_bases
TTTGCAGTATTTTGGGCTAAAAAATGATAAATTTAATAATATATAAAAATCATAATTATTAAAGATAGATGATTATTATT
TTTTTTACAATGGTGATTTA

Downstream 100 bases:

>100_bases
GATTACCATTTTATAATAAAAATTAATGAGGATTACAAAATGCCTATTGATAAGGATATTAAAAAAGTATTAATAATCGG
TTCTGGACCTATTCAAATTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MVKMAKLALEDGTVLKGDGFGYETTKVGELVFSTGMVGYTESLTDPSFKGEILMSTYPLEGNYGVSEEWYQSDKIQAEGF
VVREVCREVSNFSSQKTLDDFLNEFETPGISGIDTRDLTLKIREEGSMKAAISTEDIPDSELIELARAQQSIIDIDVVPQ
VSTKEIKVFNEDANKKVALIDCGVKKNIINNFLERGIGVVLFPYDTDYKTILNYSPDGLMITSGPGNPDRVHETISTVQK
LSERLPIFGICMGQQLVAKSFGARSYKMKFGHRGANQPVKDLKTGKVYITSQNHGFTIDKESINETDLVLTQVNLNDGTP
EGFSHKELPVHCIQYHPEAGPGPNDTRYVFDKFSQMMDEY

Sequences:

>Translated_360_residues
MVKMAKLALEDGTVLKGDGFGYETTKVGELVFSTGMVGYTESLTDPSFKGEILMSTYPLEGNYGVSEEWYQSDKIQAEGF
VVREVCREVSNFSSQKTLDDFLNEFETPGISGIDTRDLTLKIREEGSMKAAISTEDIPDSELIELARAQQSIIDIDVVPQ
VSTKEIKVFNEDANKKVALIDCGVKKNIINNFLERGIGVVLFPYDTDYKTILNYSPDGLMITSGPGNPDRVHETISTVQK
LSERLPIFGICMGQQLVAKSFGARSYKMKFGHRGANQPVKDLKTGKVYITSQNHGFTIDKESINETDLVLTQVNLNDGTP
EGFSHKELPVHCIQYHPEAGPGPNDTRYVFDKFSQMMDEY
>Mature_360_residues
MVKMAKLALEDGTVLKGDGFGYETTKVGELVFSTGMVGYTESLTDPSFKGEILMSTYPLEGNYGVSEEWYQSDKIQAEGF
VVREVCREVSNFSSQKTLDDFLNEFETPGISGIDTRDLTLKIREEGSMKAAISTEDIPDSELIELARAQQSIIDIDVVPQ
VSTKEIKVFNEDANKKVALIDCGVKKNIINNFLERGIGVVLFPYDTDYKTILNYSPDGLMITSGPGNPDRVHETISTVQK
LSERLPIFGICMGQQLVAKSFGARSYKMKFGHRGANQPVKDLKTGKVYITSQNHGFTIDKESINETDLVLTQVNLNDGTP
EGFSHKELPVHCIQYHPEAGPGPNDTRYVFDKFSQMMDEY

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=368, Percent_Identity=39.945652173913, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=35.8695652173913, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=35.8695652173913, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=37.8306878306878, Blast_Score=267, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI193204318, Length=368, Percent_Identity=38.5869565217391, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=37.8787878787879, Blast_Score=269, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6324878, Length=374, Percent_Identity=37.7005347593583, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI45555749, Length=379, Percent_Identity=41.688654353562, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642586, Length=379, Percent_Identity=41.688654353562, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40066; Mature: 40066

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMAKLALEDGTVLKGDGFGYETTKVGELVFSTGMVGYTESLTDPSFKGEILMSTYPLE
CCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC
GNYGVSEEWYQSDKIQAEGFVVREVCREVSNFSSQKTLDDFLNEFETPGISGIDTRDLTL
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
KIREEGSMKAAISTEDIPDSELIELARAQQSIIDIDVVPQVSTKEIKVFNEDANKKVALI
EEECCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCEEEEE
DCGVKKNIINNFLERGIGVVLFPYDTDYKTILNYSPDGLMITSGPGNPDRVHETISTVQK
ECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
LSERLPIFGICMGQQLVAKSFGARSYKMKFGHRGANQPVKDLKTGKVYITSQNHGFTIDK
HHHCCCEEEEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCCEEEEC
ESINETDLVLTQVNLNDGTPEGFSHKELPVHCIQYHPEAGPGPNDTRYVFDKFSQMMDEY
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKMAKLALEDGTVLKGDGFGYETTKVGELVFSTGMVGYTESLTDPSFKGEILMSTYPLE
CCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC
GNYGVSEEWYQSDKIQAEGFVVREVCREVSNFSSQKTLDDFLNEFETPGISGIDTRDLTL
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
KIREEGSMKAAISTEDIPDSELIELARAQQSIIDIDVVPQVSTKEIKVFNEDANKKVALI
EEECCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCEEEEE
DCGVKKNIINNFLERGIGVVLFPYDTDYKTILNYSPDGLMITSGPGNPDRVHETISTVQK
ECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
LSERLPIFGICMGQQLVAKSFGARSYKMKFGHRGANQPVKDLKTGKVYITSQNHGFTIDK
HHHCCCEEEEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCCEEEEC
ESINETDLVLTQVNLNDGTPEGFSHKELPVHCIQYHPEAGPGPNDTRYVFDKFSQMMDEY
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]