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The map label for this gene is argB

Identifier: 148642435

GI number: 148642435

Start: 356466

End: 357344

Strand: Reverse

Name: argB

Synonym: Msm_0375

Alternate gene names: 148642435

Gene position: 357344-356466 (Counterclockwise)

Preceding gene: 148642436

Following gene: 148642432

Centisome position: 19.28

GC content: 33.45

Gene sequence:

>879_bases
ATGAAGGACGTAAATATTTTAGTTGAGGCTTTACCTTATATTAAAAAATTCCATGAAAAAAAGATTCTGATTAAGTACGG
AGGACATGCTATGATAGATGAAGATGCAATGTCTTCTACAGTTAGGGATACTGTTCTTTTAAAATATGTGGGTATGGAAC
CACTTATTGTTCATGGTGGCGGTCCGGAAATATCCAGATCTATGGACAAGTTAGGTAAAGAGCCTAAATTCATTAAAGGA
TTAAGAGTTACTGATGAAGAAACTATGGAAATTATTGAAATGGTTTTAGTTGGTAAAATAAGTACAGATATTGTTTCTCA
GATTTCTTATCATGATGGAAAAGGCATAAGTTTATCTGGTAAAGACAGCAGATTAATTTTCGCTCATAAAAAACCTGTCA
GTAAAGTTACCAGCGAATCTGGCGGAGAAGAGGAAATTGATTTAGGTCTTGTGGGTGAAATTGATTGCATCAATACAGAT
TTACTTGAAATGTTTCTGAAAAACAATTATATTCCGGTTATATCTCCGGTAGGTATCGCCGATGATGGATCAAGTTTAAA
TTTAAATGCAGATACTGCAGCTGGTGAAATAGCTAGTTCTGTTGATGCTGAAAAGTTAATAATTTTAACTGATGTTCCGG
GAGTTTTAAGAGATCCTAATGACCCTGATTCATTAATCCAAAGGATAAAAATAGATGAAGTGCCGGACTTAATTGAAGAA
GGAGTTATTTCCGGAGGAATGATTCCAAAAATTGAAACATGTGTCAAAGCAATTGAAGACGGTGTTAAATCTTGCCATAT
CATTGACGGACGTAAAAAACATTCTCTTTTACTTGAAATTTTCACAAAAAATGGTATAGGAACTATGATTTATAAATAG

Upstream 100 bases:

>100_bases
ATTCAGATATTTGTTGACATTATAATATATTTTTTTTAAGAAATGAATAATTCCAAAGATTTAATAAAGTATTAAGTAAA
ATAATTATTGTAGTGATTAA

Downstream 100 bases:

>100_bases
TTCTTATTTTGAAAATAAAAATGAAATATAATCTATAAATGATTATATTCTTCTCTTAATTTTCTTCTAAGCAGTTTCCA
GCCATTAACTCTTGGAAGCT

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKDVNILVEALPYIKKFHEKKILIKYGGHAMIDEDAMSSTVRDTVLLKYVGMEPLIVHGGGPEISRSMDKLGKEPKFIKG
LRVTDEETMEIIEMVLVGKISTDIVSQISYHDGKGISLSGKDSRLIFAHKKPVSKVTSESGGEEEIDLGLVGEIDCINTD
LLEMFLKNNYIPVISPVGIADDGSSLNLNADTAAGEIASSVDAEKLIILTDVPGVLRDPNDPDSLIQRIKIDEVPDLIEE
GVISGGMIPKIETCVKAIEDGVKSCHIIDGRKKHSLLLEIFTKNGIGTMIYK

Sequences:

>Translated_292_residues
MKDVNILVEALPYIKKFHEKKILIKYGGHAMIDEDAMSSTVRDTVLLKYVGMEPLIVHGGGPEISRSMDKLGKEPKFIKG
LRVTDEETMEIIEMVLVGKISTDIVSQISYHDGKGISLSGKDSRLIFAHKKPVSKVTSESGGEEEIDLGLVGEIDCINTD
LLEMFLKNNYIPVISPVGIADDGSSLNLNADTAAGEIASSVDAEKLIILTDVPGVLRDPNDPDSLIQRIKIDEVPDLIEE
GVISGGMIPKIETCVKAIEDGVKSCHIIDGRKKHSLLLEIFTKNGIGTMIYK
>Mature_292_residues
MKDVNILVEALPYIKKFHEKKILIKYGGHAMIDEDAMSSTVRDTVLLKYVGMEPLIVHGGGPEISRSMDKLGKEPKFIKG
LRVTDEETMEIIEMVLVGKISTDIVSQISYHDGKGISLSGKDSRLIFAHKKPVSKVTSESGGEEEIDLGLVGEIDCINTD
LLEMFLKNNYIPVISPVGIADDGSSLNLNADTAAGEIASSVDAEKLIILTDVPGVLRDPNDPDSLIQRIKIDEVPDLIEE
GVISGGMIPKIETCVKAIEDGVKSCHIIDGRKKHSLLLEIFTKNGIGTMIYK

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=290, Percent_Identity=29.6551724137931, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693208, Length=270, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_METS3 (A5UK52)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272948.1
- ProteinModelPortal:   A5UK52
- SMR:   A5UK52
- STRING:   A5UK52
- GeneID:   5216530
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0375
- eggNOG:   arNOG04941
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- ProtClustDB:   PRK00942
- GO:   GO:0005737
- HAMAP:   MF_00082_A
- InterPro:   IPR004662
- InterPro:   IPR001048
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 31825; Mature: 31825

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVNILVEALPYIKKFHEKKILIKYGGHAMIDEDAMSSTVRDTVLLKYVGMEPLIVHGG
CCHHHHHHHHHHHHHHHHCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCEEEEECC
GPEISRSMDKLGKEPKFIKGLRVTDEETMEIIEMVLVGKISTDIVSQISYHDGKGISLSG
CCHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
KDSRLIFAHKKPVSKVTSESGGEEEIDLGLVGEIDCINTDLLEMFLKNNYIPVISPVGIA
CCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCCC
DDGSSLNLNADTAAGEIASSVDAEKLIILTDVPGVLRDPNDPDSLIQRIKIDEVPDLIEE
CCCCEEEECCCHHHHHHHHCCCCCEEEEEECCCHHHCCCCCHHHHHHHHCHHHHHHHHHH
GVISGGMIPKIETCVKAIEDGVKSCHIIDGRKKHSLLLEIFTKNGIGTMIYK
HCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MKDVNILVEALPYIKKFHEKKILIKYGGHAMIDEDAMSSTVRDTVLLKYVGMEPLIVHGG
CCHHHHHHHHHHHHHHHHCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCEEEEECC
GPEISRSMDKLGKEPKFIKGLRVTDEETMEIIEMVLVGKISTDIVSQISYHDGKGISLSG
CCHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
KDSRLIFAHKKPVSKVTSESGGEEEIDLGLVGEIDCINTDLLEMFLKNNYIPVISPVGIA
CCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCCC
DDGSSLNLNADTAAGEIASSVDAEKLIILTDVPGVLRDPNDPDSLIQRIKIDEVPDLIEE
CCCCEEEECCCHHHHHHHHCCCCCEEEEEECCCHHHCCCCCHHHHHHHHCHHHHHHHHHH
GVISGGMIPKIETCVKAIEDGVKSCHIIDGRKKHSLLLEIFTKNGIGTMIYK
HCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA