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The map label for this gene is cimA [H]

Identifier: 148642410

GI number: 148642410

Start: 328189

End: 329661

Strand: Reverse

Name: cimA [H]

Synonym: Msm_0350

Alternate gene names: 148642410

Gene position: 329661-328189 (Counterclockwise)

Preceding gene: 148642412

Following gene: 148642409

Centisome position: 17.79

GC content: 34.22

Gene sequence:

>1473_bases
ATGAATATTAAAATATTGGACACTACTTTAAGAGATGGAGAACAGACTCCTGGTGTATCTTTAACTTCATTGGAGAAATT
AAGAATTGCTACTAAACTTGATGAAATTGGTGTTAATTTTATTGAGGCAGGTTCAGCAATTACTTCAGAAGGGGAAAGAC
AGTCTATTAAAGATATAACTCAACAGAATTTTAATGCAGAGATTCTTAGTTTTTCCAGACCTCTTGAAATGGATATTGAT
TATTGTTTAGAATGTGATGTTGATGCAGTTAATTTAGTTGTTCCTACTTCTGATTTGCATATTTTTGATAAATTAAAAAT
TACTAAAGAGGAGTTGCTGGCAATGTCCAACTCTGCAGTTGATTATTGTAAGGATCATGGGTTAACTGTTGAGCTGTCTG
CTGAAGATGCATCCCGAACAAGCATTGATTTTTTAAAGACAGTTTATGTTAATGCTATTGAACATGGTGCTGATAGGGTT
TGCTTATGTGATACTGTTGGTATTTTAACACCTGAATCTTCTTTTGAAATGTTTAAGCAATTAAAAGAAGATATTGATGT
TCCAATTAGCTGTCATTGTCATAATGATTTTGGTTTGGCTGTTGCCAATACATTTGCCGCTTTGAAAGGGGGAGCATCTG
AATTCCATTCTACCATAAATGGTATCGGTGAGAGAGCAGGCAATACTTCTTTTGAAGAATGTGTTGTTGGCATATACAAA
TTATTCCCTCAGTTTTCAACAGATATTAAAATTCATGAGATTTACACCATTTCAAAATTAGTGGCAAGATTGACTGGTGT
TTATATTCAGCCTAACAAAGCAATTGTAGGTGAGAATGCTTTTGCTCATGAATCAGGAATTCATTCTGATGGAATTATTA
AAAATGCCGCTACTTATGAATCTATAACTCCTGAACTTGTTGGCCGTAAACGCAGGTTCGTCATTGGTAAGCATATGGGT
ACTCATGGTTTGGATGTTAGACTAAAAGAGCTGGGAGTTAATGTCAGTAAAAATCAGCTTAAAAAGATTTGCGACAATAT
TAAAGTTCTTGCTGATAAAGGTAAAACAGTTACTGATGTTGATTTGCAGGCAATAGCTGATAATGTTTTAGAAATTAATC
ATAAAGACAGGATTAAGTTAAATGAGGTTACTATTGTATCAGGTAATAAGGTTATGCCTACAGCATCTGTAAAACTGACA
GTTGACGGTGAGGAAGTTTTAAATGCTGGTGTAGGTATTGGTCCTGTGGATGCTGCAATCAATGCTGTTAACTCTTTGGA
TATCTTTGATGATATTGATTTTATTGAATACCATGTAGATGCAATTACCGGAGGTACTGATGCACTTATTGATGTAATTA
TTAAACTTCAGAAAGGGGATAAAATCATATCTGCAAGAGGAACTGAGCCGGATATTATTAATGCCAGTGTAAAGGCATAT
ATTTCTGGTGTTAACCGCTTACTTTCTAATTAG

Upstream 100 bases:

>100_bases
CTGCAAGCCTTTTTCTATTACATTACTTACTTATTTTTTAATATTTTTATTTTATCTGTTTTTTTTTTTATTTCATGATT
ATTTTGTCTGCGGTGATTTT

Downstream 100 bases:

>100_bases
GGTGTTTTTATGGAAATTAAAGTTTTAGGATTTAGAGGAAATATTGATTCTGTCAGCAAAACACTGGATAAAATTAACAG
TCTTAAAAATAGTGATGATG

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNIKILDTTLRDGEQTPGVSLTSLEKLRIATKLDEIGVNFIEAGSAITSEGERQSIKDITQQNFNAEILSFSRPLEMDID
YCLECDVDAVNLVVPTSDLHIFDKLKITKEELLAMSNSAVDYCKDHGLTVELSAEDASRTSIDFLKTVYVNAIEHGADRV
CLCDTVGILTPESSFEMFKQLKEDIDVPISCHCHNDFGLAVANTFAALKGGASEFHSTINGIGERAGNTSFEECVVGIYK
LFPQFSTDIKIHEIYTISKLVARLTGVYIQPNKAIVGENAFAHESGIHSDGIIKNAATYESITPELVGRKRRFVIGKHMG
THGLDVRLKELGVNVSKNQLKKICDNIKVLADKGKTVTDVDLQAIADNVLEINHKDRIKLNEVTIVSGNKVMPTASVKLT
VDGEEVLNAGVGIGPVDAAINAVNSLDIFDDIDFIEYHVDAITGGTDALIDVIIKLQKGDKIISARGTEPDIINASVKAY
ISGVNRLLSN

Sequences:

>Translated_490_residues
MNIKILDTTLRDGEQTPGVSLTSLEKLRIATKLDEIGVNFIEAGSAITSEGERQSIKDITQQNFNAEILSFSRPLEMDID
YCLECDVDAVNLVVPTSDLHIFDKLKITKEELLAMSNSAVDYCKDHGLTVELSAEDASRTSIDFLKTVYVNAIEHGADRV
CLCDTVGILTPESSFEMFKQLKEDIDVPISCHCHNDFGLAVANTFAALKGGASEFHSTINGIGERAGNTSFEECVVGIYK
LFPQFSTDIKIHEIYTISKLVARLTGVYIQPNKAIVGENAFAHESGIHSDGIIKNAATYESITPELVGRKRRFVIGKHMG
THGLDVRLKELGVNVSKNQLKKICDNIKVLADKGKTVTDVDLQAIADNVLEINHKDRIKLNEVTIVSGNKVMPTASVKLT
VDGEEVLNAGVGIGPVDAAINAVNSLDIFDDIDFIEYHVDAITGGTDALIDVIIKLQKGDKIISARGTEPDIINASVKAY
ISGVNRLLSN
>Mature_490_residues
MNIKILDTTLRDGEQTPGVSLTSLEKLRIATKLDEIGVNFIEAGSAITSEGERQSIKDITQQNFNAEILSFSRPLEMDID
YCLECDVDAVNLVVPTSDLHIFDKLKITKEELLAMSNSAVDYCKDHGLTVELSAEDASRTSIDFLKTVYVNAIEHGADRV
CLCDTVGILTPESSFEMFKQLKEDIDVPISCHCHNDFGLAVANTFAALKGGASEFHSTINGIGERAGNTSFEECVVGIYK
LFPQFSTDIKIHEIYTISKLVARLTGVYIQPNKAIVGENAFAHESGIHSDGIIKNAATYESITPELVGRKRRFVIGKHMG
THGLDVRLKELGVNVSKNQLKKICDNIKVLADKGKTVTDVDLQAIADNVLEINHKDRIKLNEVTIVSGNKVMPTASVKLT
VDGEEVLNAGVGIGPVDAAINAVNSLDIFDDIDFIEYHVDAITGGTDALIDVIIKLQKGDKIISARGTEPDIINASVKAY
ISGVNRLLSN

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=35.8870967741936, Blast_Score=265, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=32.7868852459016, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=32.7868852459016, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324682, Length=477, Percent_Identity=22.8511530398323, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 53319; Mature: 53319

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKILDTTLRDGEQTPGVSLTSLEKLRIATKLDEIGVNFIEAGSAITSEGERQSIKDIT
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHH
QQNFNAEILSFSRPLEMDIDYCLECDVDAVNLVVPTSDLHIFDKLKITKEELLAMSNSAV
HCCCCCEEEECCCCCCCCCHHEEECCCCEEEEEEECCCCEEEHHHHCCHHHHHHHCCCHH
DYCKDHGLTVELSAEDASRTSIDFLKTVYVNAIEHGADRVCLCDTVGILTPESSFEMFKQ
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
LKEDIDVPISCHCHNDFGLAVANTFAALKGGASEFHSTINGIGERAGNTSFEECVVGIYK
HHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFPQFSTDIKIHEIYTISKLVARLTGVYIQPNKAIVGENAFAHESGIHSDGIIKNAATYE
HCCCCCCCEEEEHHHHHHHHHHHHHCEEECCCCEEECCCCCHHCCCCCCCCCCCCCCHHH
SITPELVGRKRRFVIGKHMGTHGLDVRLKELGVNVSKNQLKKICDNIKVLADKGKTVTDV
CCCHHHHCCCCEEEEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHEEEECCCCEEEEC
DLQAIADNVLEINHKDRIKLNEVTIVSGNKVMPTASVKLTVDGEEVLNAGVGIGPVDAAI
HHHHHHHHHHCCCCCCCEEEEEEEEEECCEECCCCEEEEEECHHHHHHCCCCCCCHHHHH
NAVNSLDIFDDIDFIEYHVDAITGGTDALIDVIIKLQKGDKIISARGTEPDIINASVKAY
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHH
ISGVNRLLSN
HHHHHHHHCC
>Mature Secondary Structure
MNIKILDTTLRDGEQTPGVSLTSLEKLRIATKLDEIGVNFIEAGSAITSEGERQSIKDIT
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHH
QQNFNAEILSFSRPLEMDIDYCLECDVDAVNLVVPTSDLHIFDKLKITKEELLAMSNSAV
HCCCCCEEEECCCCCCCCCHHEEECCCCEEEEEEECCCCEEEHHHHCCHHHHHHHCCCHH
DYCKDHGLTVELSAEDASRTSIDFLKTVYVNAIEHGADRVCLCDTVGILTPESSFEMFKQ
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
LKEDIDVPISCHCHNDFGLAVANTFAALKGGASEFHSTINGIGERAGNTSFEECVVGIYK
HHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFPQFSTDIKIHEIYTISKLVARLTGVYIQPNKAIVGENAFAHESGIHSDGIIKNAATYE
HCCCCCCCEEEEHHHHHHHHHHHHHCEEECCCCEEECCCCCHHCCCCCCCCCCCCCCHHH
SITPELVGRKRRFVIGKHMGTHGLDVRLKELGVNVSKNQLKKICDNIKVLADKGKTVTDV
CCCHHHHCCCCEEEEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHEEEECCCCEEEEC
DLQAIADNVLEINHKDRIKLNEVTIVSGNKVMPTASVKLTVDGEEVLNAGVGIGPVDAAI
HHHHHHHHHHCCCCCCCEEEEEEEEEECCEECCCCEEEEEECHHHHHHCCCCCCCHHHHH
NAVNSLDIFDDIDFIEYHVDAITGGTDALIDVIIKLQKGDKIISARGTEPDIINASVKAY
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHH
ISGVNRLLSN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]