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The map label for this gene is aroA

Identifier: 148642333

GI number: 148642333

Start: 260742

End: 262058

Strand: Reverse

Name: aroA

Synonym: Msm_0273

Alternate gene names: 148642333

Gene position: 262058-260742 (Counterclockwise)

Preceding gene: 148642334

Following gene: 148642332

Centisome position: 14.14

GC content: 33.64

Gene sequence:

>1317_bases
ATGAATCTTAAAGTTAAAACTATTTCCAATATTGGTGGTGAAGTTAAGGCACCACCCTCTAAAAGTTATTCTCACAGGGC
AGTTATATTAGCTTCTTTAGCTGATGGAACATCCAAAATTCATGATATGCTTTTTTCACAGGATGTTTTGTCATCCATTA
ATGTATGCAGAGCTTTAGGAGCAAATATCACTAAAAAAGATGATTATTTGGAAGTGATCGGAACCAATGGTAAATTGCAC
AATTCATCAGAAGTTCCTATTGATTTAGGTAATTCCGGAACTACTTTAAGATTAATGACAAGCATAGCCAGTTTGGCTGA
CAATGAAGTTATATTAACAGGAGATGACTCTTTGCAGACAAGGCCTATGGAGATTTTAACAGAATCATTGGCTTCATTAG
GTGTATGTGCAGCTTCAATTAATGGTAATGGGAAAGCACCTATTTTAATCAAGCCAGGTTATGTGGGTGGTGAAACTAAT
ATTTTAGGCAATGTCAGTTCTCAGTTTATTTCATCAATTCTGATTTCTGCTCCTTTATCTGAAAATGGAGTTGATTTATT
TGTACTGCCTGAATTCAAGTCACGCCCTTATGTAAATATGACCTGTGATATAATGGCTAAATTTGGTGTTAAAATTGAAA
ATGAATTCTTTGTACGTCATGATGACTGTGACAGGGAATCTAAAAATTGCCGCATTGATGAATTTAAAATATCTAAACAG
GAATATAAATCCTGTGACTATGTAGTTGAAGGCGACTATTCATCAGCATCTTATTTGCTTGCAGCTGTAGCTATTTATGG
TGGAAATGCAAAAATATTAAATTTATTTAAAGATTCTAAACAGGGAGATAAGTTAATATTGAATATTTTAAAGAAGATGG
GTGCAAAAATAGAGATTTTTGATGATTATGTGGAAATCTCATCTGAAGGTAATTTAAAAGGTATTGATGTTGATTTGTCA
AATGCTCCTGATTTATTAATTACAGTAGCTATTTTAGCAGCTCTGGCTGATGGAACCACTAATATTACAGGTGTTAAACA
TGCCAGAGTTAAAGAAACAGATAGGATAGCTACTACATGTAGTGAACTTAAGAAATTAGGCTGCAAGTTAAAAGAATTTG
AAGACGGCATGTCTATTGAAGGAGGAATCCGTTCAGGTGTTGTTGATTCACATAAAGATCATAGGCTGGCTATGGCATTT
TCATTGGTTGGTCTAAAACATGATATAGAAATTAAAAATGGGGAAGTTTTTGATGTGTCTTTTCCTAATTTCATTGAAGC
CATGTCAGAAATTGGAGTTGAACTGGAGTTAATTTAG

Upstream 100 bases:

>100_bases
GTTAAATAATTTAATTGAAAAAAAACCTCACTTCATTTCTTTTTTTCAAATTTTTTACTTTTCAGATAAATATAATAATT
TATAAAATATAATTATCAGT

Downstream 100 bases:

>100_bases
GAAGTGTTGGTGTTGGAATTAACAGAAACTGAAAAGGTTATTAAAATTGTAGATGAGTTAAATAAGTTATATAAAATCAG
GGAGTTTGTAAATAAGGATC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNLKVKTISNIGGEVKAPPSKSYSHRAVILASLADGTSKIHDMLFSQDVLSSINVCRALGANITKKDDYLEVIGTNGKLH
NSSEVPIDLGNSGTTLRLMTSIASLADNEVILTGDDSLQTRPMEILTESLASLGVCAASINGNGKAPILIKPGYVGGETN
ILGNVSSQFISSILISAPLSENGVDLFVLPEFKSRPYVNMTCDIMAKFGVKIENEFFVRHDDCDRESKNCRIDEFKISKQ
EYKSCDYVVEGDYSSASYLLAAVAIYGGNAKILNLFKDSKQGDKLILNILKKMGAKIEIFDDYVEISSEGNLKGIDVDLS
NAPDLLITVAILAALADGTTNITGVKHARVKETDRIATTCSELKKLGCKLKEFEDGMSIEGGIRSGVVDSHKDHRLAMAF
SLVGLKHDIEIKNGEVFDVSFPNFIEAMSEIGVELELI

Sequences:

>Translated_438_residues
MNLKVKTISNIGGEVKAPPSKSYSHRAVILASLADGTSKIHDMLFSQDVLSSINVCRALGANITKKDDYLEVIGTNGKLH
NSSEVPIDLGNSGTTLRLMTSIASLADNEVILTGDDSLQTRPMEILTESLASLGVCAASINGNGKAPILIKPGYVGGETN
ILGNVSSQFISSILISAPLSENGVDLFVLPEFKSRPYVNMTCDIMAKFGVKIENEFFVRHDDCDRESKNCRIDEFKISKQ
EYKSCDYVVEGDYSSASYLLAAVAIYGGNAKILNLFKDSKQGDKLILNILKKMGAKIEIFDDYVEISSEGNLKGIDVDLS
NAPDLLITVAILAALADGTTNITGVKHARVKETDRIATTCSELKKLGCKLKEFEDGMSIEGGIRSGVVDSHKDHRLAMAF
SLVGLKHDIEIKNGEVFDVSFPNFIEAMSEIGVELELI
>Mature_438_residues
MNLKVKTISNIGGEVKAPPSKSYSHRAVILASLADGTSKIHDMLFSQDVLSSINVCRALGANITKKDDYLEVIGTNGKLH
NSSEVPIDLGNSGTTLRLMTSIASLADNEVILTGDDSLQTRPMEILTESLASLGVCAASINGNGKAPILIKPGYVGGETN
ILGNVSSQFISSILISAPLSENGVDLFVLPEFKSRPYVNMTCDIMAKFGVKIENEFFVRHDDCDRESKNCRIDEFKISKQ
EYKSCDYVVEGDYSSASYLLAAVAIYGGNAKILNLFKDSKQGDKLILNILKKMGAKIEIFDDYVEISSEGNLKGIDVDLS
NAPDLLITVAILAALADGTTNITGVKHARVKETDRIATTCSELKKLGCKLKEFEDGMSIEGGIRSGVVDSHKDHRLAMAF
SLVGLKHDIEIKNGEVFDVSFPNFIEAMSEIGVELELI

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=437, Percent_Identity=34.324942791762, Blast_Score=247, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6320332, Length=469, Percent_Identity=32.1961620469083, Blast_Score=188, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_METS3 (A5UJV0)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272846.1
- STRING:   A5UJV0
- GeneID:   5216756
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0273
- eggNOG:   arNOG05032
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47384; Mature: 47384

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVKTISNIGGEVKAPPSKSYSHRAVILASLADGTSKIHDMLFSQDVLSSINVCRALG
CCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
ANITKKDDYLEVIGTNGKLHNSSEVPIDLGNSGTTLRLMTSIASLADNEVILTGDDSLQT
CCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
RPMEILTESLASLGVCAASINGNGKAPILIKPGYVGGETNILGNVSSQFISSILISAPLS
CHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCC
ENGVDLFVLPEFKSRPYVNMTCDIMAKFGVKIENEFFVRHDDCDRESKNCRIDEFKISKQ
CCCCEEEEECCCCCCCEEEEEHHHHHHCCCEECCEEEEECCCCCCCCCCCEEEEEECCHH
EYKSCDYVVEGDYSSASYLLAAVAIYGGNAKILNLFKDSKQGDKLILNILKKMGAKIEIF
HHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEE
DDYVEISSEGNLKGIDVDLSNAPDLLITVAILAALADGTTNITGVKHARVKETDRIATTC
EEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SELKKLGCKLKEFEDGMSIEGGIRSGVVDSHKDHRLAMAFSLVGLKHDIEIKNGEVFDVS
HHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCEEEEEECCCEEEEEC
FPNFIEAMSEIGVELELI
CHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MNLKVKTISNIGGEVKAPPSKSYSHRAVILASLADGTSKIHDMLFSQDVLSSINVCRALG
CCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
ANITKKDDYLEVIGTNGKLHNSSEVPIDLGNSGTTLRLMTSIASLADNEVILTGDDSLQT
CCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
RPMEILTESLASLGVCAASINGNGKAPILIKPGYVGGETNILGNVSSQFISSILISAPLS
CHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCC
ENGVDLFVLPEFKSRPYVNMTCDIMAKFGVKIENEFFVRHDDCDRESKNCRIDEFKISKQ
CCCCEEEEECCCCCCCEEEEEHHHHHHCCCEECCEEEEECCCCCCCCCCCEEEEEECCHH
EYKSCDYVVEGDYSSASYLLAAVAIYGGNAKILNLFKDSKQGDKLILNILKKMGAKIEIF
HHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEE
DDYVEISSEGNLKGIDVDLSNAPDLLITVAILAALADGTTNITGVKHARVKETDRIATTC
EEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SELKKLGCKLKEFEDGMSIEGGIRSGVVDSHKDHRLAMAFSLVGLKHDIEIKNGEVFDVS
HHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCEEEEEECCCEEEEEC
FPNFIEAMSEIGVELELI
CHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA