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The map label for this gene is nth [H]

Identifier: 148642332

GI number: 148642332

Start: 260106

End: 260738

Strand: Reverse

Name: nth [H]

Synonym: Msm_0272

Alternate gene names: 148642332

Gene position: 260738-260106 (Counterclockwise)

Preceding gene: 148642333

Following gene: 148642331

Centisome position: 14.07

GC content: 34.76

Gene sequence:

>633_bases
GTGTTGGTGTTGGAATTAACAGAAACTGAAAAGGTTATTAAAATTGTAGATGAGTTAAATAAGTTATATAAAATCAGGGA
GTTTGTAAATAAGGATCCCTACAAAGTTTTAGTCAGGACAATTTTATCTCAAAGAACAAGAGATGAAAATACAGACCAGG
CTACTAAAAACTTATTTGGCAAATATAAAAACATTTATGAAATAGTTGATGCTCCAACTGATGATGTTGAAGAGCTAATT
AGATGTTCAGGTTTTTATCGTGTAAAAGCAGCTAGAATTAAAGAGGTTTCAAGGATTTTGATTGATCAGTATGGTGGTGA
AGTTCCGGACAATCTAAAGGAGTTGGTTGAGCTTCCTGGTGTTGGCCGTAAAACTGCCAATTGTGTTCTGGTTTATGCAT
TTGAACTTCCGGCTATTCCTGTTGACACTCATGTTCACAGAATTTCCAACAGAATTGGTTTGGTAAATACCAAAACACCT
GAACAGACAGAAGTGGAATTGGCTAAAATTGCCCCTAAGGAATTATGGATTAAATTAAATGATTTGATGGTTCAGTTTGG
TCAAACTATCTGTAAACCTATGAGTCCTCAATGTGAAATGTGTCCGATTTCAGATATTTGTGATTACTGCTGA

Upstream 100 bases:

>100_bases
TGATATAGAAATTAAAAATGGGGAAGTTTTTGATGTGTCTTTTCCTAATTTCATTGAAGCCATGTCAGAAATTGGAGTTG
AACTGGAGTTAATTTAGGAA

Downstream 100 bases:

>100_bases
TTTTCCGAAACATTTATAAATAAAAACATTGTTATATAACAGTAGTTATAAACAATAACTATTGTTATATTAGGTATTCC
AAAATATGACAATTAATGAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MLVLELTETEKVIKIVDELNKLYKIREFVNKDPYKVLVRTILSQRTRDENTDQATKNLFGKYKNIYEIVDAPTDDVEELI
RCSGFYRVKAARIKEVSRILIDQYGGEVPDNLKELVELPGVGRKTANCVLVYAFELPAIPVDTHVHRISNRIGLVNTKTP
EQTEVELAKIAPKELWIKLNDLMVQFGQTICKPMSPQCEMCPISDICDYC

Sequences:

>Translated_210_residues
MLVLELTETEKVIKIVDELNKLYKIREFVNKDPYKVLVRTILSQRTRDENTDQATKNLFGKYKNIYEIVDAPTDDVEELI
RCSGFYRVKAARIKEVSRILIDQYGGEVPDNLKELVELPGVGRKTANCVLVYAFELPAIPVDTHVHRISNRIGLVNTKTP
EQTEVELAKIAPKELWIKLNDLMVQFGQTICKPMSPQCEMCPISDICDYC
>Mature_210_residues
MLVLELTETEKVIKIVDELNKLYKIREFVNKDPYKVLVRTILSQRTRDENTDQATKNLFGKYKNIYEIVDAPTDDVEELI
RCSGFYRVKAARIKEVSRILIDQYGGEVPDNLKELVELPGVGRKTANCVLVYAFELPAIPVDTHVHRISNRIGLVNTKTP
EQTEVELAKIAPKELWIKLNDLMVQFGQTICKPMSPQCEMCPISDICDYC

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=39.5480225988701, Blast_Score=134, Evalue=4e-32,
Organism=Homo sapiens, GI115298648, Length=192, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI6912520, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI190358497, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298654, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298652, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298650, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1787920, Length=190, Percent_Identity=34.7368421052632, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1789331, Length=185, Percent_Identity=25.9459459459459, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=43.1952662721894, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=34.7593582887701, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319304, Length=172, Percent_Identity=35.4651162790698, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=43.5028248587571, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24063; Mature: 24063

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLELTETEKVIKIVDELNKLYKIREFVNKDPYKVLVRTILSQRTRDENTDQATKNLFG
CEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KYKNIYEIVDAPTDDVEELIRCSGFYRVKAARIKEVSRILIDQYGGEVPDNLKELVELPG
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
VGRKTANCVLVYAFELPAIPVDTHVHRISNRIGLVNTKTPEQTEVELAKIAPKELWIKLN
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHH
DLMVQFGQTICKPMSPQCEMCPISDICDYC
HHHHHHHHHHHCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MLVLELTETEKVIKIVDELNKLYKIREFVNKDPYKVLVRTILSQRTRDENTDQATKNLFG
CEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KYKNIYEIVDAPTDDVEELIRCSGFYRVKAARIKEVSRILIDQYGGEVPDNLKELVELPG
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
VGRKTANCVLVYAFELPAIPVDTHVHRISNRIGLVNTKTPEQTEVELAKIAPKELWIKLN
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHH
DLMVQFGQTICKPMSPQCEMCPISDICDYC
HHHHHHHHHHHCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]