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The map label for this gene is cysK [H]

Identifier: 148642331

GI number: 148642331

Start: 259010

End: 259957

Strand: Reverse

Name: cysK [H]

Synonym: Msm_0271

Alternate gene names: 148642331

Gene position: 259957-259010 (Counterclockwise)

Preceding gene: 148642332

Following gene: 148642330

Centisome position: 14.03

GC content: 33.76

Gene sequence:

>948_bases
ATGGTAAATGTCCCAGAATTAAAAAGAGGAGTTTTAGATAGTGTTATTGATGCTATTGGAAATACTCCTATTGTAAGATT
AAATAATATAACTGAAGATTTAGATGCAGAAGTAGATGTAAAAATGGAATCTTTCAATCCAACTGGAAGTGTAAAAGACC
GTGTTGCAGTTGCAATGATTGAAGATGCTGAAGAAAAAGGTTTGCTTAAAGAAGGTTCTGTAATTATTGAACCAACTAGT
GGAAATACCGGTATTGGATTAGGTTTTGCTGCAGCTGCTAAAGGTTACAGATTAATTTTAACAATGCCTGAAACCATGTC
TATTGAAAGAAGGAAATTGTTAGGTATCTTTGGAGCAGAATTAGTATTAACTCCAGGTTCAGAAGGTATGGGTGGAGCTA
TAGCTAAAGCTGATGAATTGGAAAAAGAAATTCCAAATGGTATTGTTTTACATCAATTTGATAATGAAGCTAATACAAGA
ATTCATTCTCAAACTACTGCTAAAGAAATTTTAAGAGATACTGAAGGTAAAATTGATGCTGTAGTTGCTGGTGTAGGAAC
TGGGGGAACTATTACAGGTATTGGAAAAGTATTAAAAGAGGAAAATCCTGATGTTCAAATTATAGCTGTTGAACCTGAGT
CTTCCCAAACCTTAGGAAAAGGAGTTAAAGGTCCTCATAAAATCCAAGGTATTGGTGCAGGTTTTGTTCCGTCTATTTAT
GATTCTGATATTATTGATGAGATTATTCCTGTTAAAGATGAAGATGCAGGTAACACTTTACTTGATTTAGCTAAAAAAGA
AGGAATCTTTGCAGGAATTTCATCTGGAGCAGCAACATGGGCTGCAATTCAAGTAGCTAAAAGGGATGAAAATAAAGGTA
AAAGAATATTGGCTATTTTACCTGATGACGGTTCAAGATATTTATCTGTTGACTGGTTATTTGAATAA

Upstream 100 bases:

>100_bases
TAAACAATAACTATTGTTATATTAGGTATTCCAAAATATGACAATTAATGATTAACCCTTTATAATATACCTTAAATGGA
ATTAATTAGGAGAAATAATT

Downstream 100 bases:

>100_bases
ATTTATATATTATGTATTATAGAAAATGTATATACTATATGTTATATTTTTATAGATATACATATTCTAAATACAATTTG
GGGTTTGTTAATGCTTGGTA

Product: cysteine synthase, CysK

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MVNVPELKRGVLDSVIDAIGNTPIVRLNNITEDLDAEVDVKMESFNPTGSVKDRVAVAMIEDAEEKGLLKEGSVIIEPTS
GNTGIGLGFAAAAKGYRLILTMPETMSIERRKLLGIFGAELVLTPGSEGMGGAIAKADELEKEIPNGIVLHQFDNEANTR
IHSQTTAKEILRDTEGKIDAVVAGVGTGGTITGIGKVLKEENPDVQIIAVEPESSQTLGKGVKGPHKIQGIGAGFVPSIY
DSDIIDEIIPVKDEDAGNTLLDLAKKEGIFAGISSGAATWAAIQVAKRDENKGKRILAILPDDGSRYLSVDWLFE

Sequences:

>Translated_315_residues
MVNVPELKRGVLDSVIDAIGNTPIVRLNNITEDLDAEVDVKMESFNPTGSVKDRVAVAMIEDAEEKGLLKEGSVIIEPTS
GNTGIGLGFAAAAKGYRLILTMPETMSIERRKLLGIFGAELVLTPGSEGMGGAIAKADELEKEIPNGIVLHQFDNEANTR
IHSQTTAKEILRDTEGKIDAVVAGVGTGGTITGIGKVLKEENPDVQIIAVEPESSQTLGKGVKGPHKIQGIGAGFVPSIY
DSDIIDEIIPVKDEDAGNTLLDLAKKEGIFAGISSGAATWAAIQVAKRDENKGKRILAILPDDGSRYLSVDWLFE
>Mature_315_residues
MVNVPELKRGVLDSVIDAIGNTPIVRLNNITEDLDAEVDVKMESFNPTGSVKDRVAVAMIEDAEEKGLLKEGSVIIEPTS
GNTGIGLGFAAAAKGYRLILTMPETMSIERRKLLGIFGAELVLTPGSEGMGGAIAKADELEKEIPNGIVLHQFDNEANTR
IHSQTTAKEILRDTEGKIDAVVAGVGTGGTITGIGKVLKEENPDVQIIAVEPESSQTLGKGVKGPHKIQGIGAGFVPSIY
DSDIIDEIIPVKDEDAGNTLLDLAKKEGIFAGISSGAATWAAIQVAKRDENKGKRILAILPDDGSRYLSVDWLFE

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=42.0062695924765, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=42.0062695924765, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=42.0062695924765, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI11345492, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788754, Length=300, Percent_Identity=50, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=41.4715719063545, Blast_Score=224, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=49.6644295302013, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI115535073, Length=292, Percent_Identity=52.7397260273973, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=296, Percent_Identity=47.2972972972973, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=48.122866894198, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17561720, Length=296, Percent_Identity=44.2567567567568, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI32566672, Length=210, Percent_Identity=46.6666666666667, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17534315, Length=322, Percent_Identity=38.8198757763975, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=326, Percent_Identity=37.1165644171779, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=116, Percent_Identity=38.7931034482759, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71996324, Length=306, Percent_Identity=25.8169934640523, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=322, Percent_Identity=39.7515527950311, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321449, Length=341, Percent_Identity=36.0703812316716, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24643623, Length=329, Percent_Identity=40.4255319148936, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI20129101, Length=329, Percent_Identity=40.4255319148936, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33394; Mature: 33394

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVPELKRGVLDSVIDAIGNTPIVRLNNITEDLDAEVDVKMESFNPTGSVKDRVAVAMI
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCHHEEEEEE
EDAEEKGLLKEGSVIIEPTSGNTGIGLGFAAAAKGYRLILTMPETMSIERRKLLGIFGAE
ECHHHCCCEECCCEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHEEECCE
LVLTPGSEGMGGAIAKADELEKEIPNGIVLHQFDNEANTRIHSQTTAKEILRDTEGKIDA
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECHHHHHHHHHCCCCCEEE
VVAGVGTGGTITGIGKVLKEENPDVQIIAVEPESSQTLGKGVKGPHKIQGIGAGFVPSIY
EEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCCCEEECCCCCCCCCC
DSDIIDEIIPVKDEDAGNTLLDLAKKEGIFAGISSGAATWAAIQVAKRDENKGKRILAIL
CHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCEEEEEE
PDDGSRYLSVDWLFE
CCCCCEEEEEEEEEC
>Mature Secondary Structure
MVNVPELKRGVLDSVIDAIGNTPIVRLNNITEDLDAEVDVKMESFNPTGSVKDRVAVAMI
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCHHEEEEEE
EDAEEKGLLKEGSVIIEPTSGNTGIGLGFAAAAKGYRLILTMPETMSIERRKLLGIFGAE
ECHHHCCCEECCCEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHEEECCE
LVLTPGSEGMGGAIAKADELEKEIPNGIVLHQFDNEANTRIHSQTTAKEILRDTEGKIDA
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECHHHHHHHHHCCCCCEEE
VVAGVGTGGTITGIGKVLKEENPDVQIIAVEPESSQTLGKGVKGPHKIQGIGAGFVPSIY
EEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCCCEEECCCCCCCCCC
DSDIIDEIIPVKDEDAGNTLLDLAKKEGIFAGISSGAATWAAIQVAKRDENKGKRILAIL
CHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCEEEEEE
PDDGSRYLSVDWLFE
CCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]