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The map label for this gene is mdeA [H]

Identifier: 148642325

GI number: 148642325

Start: 247746

End: 249044

Strand: Reverse

Name: mdeA [H]

Synonym: Msm_0265

Alternate gene names: 148642325

Gene position: 249044-247746 (Counterclockwise)

Preceding gene: 148642326

Following gene: 148642324

Centisome position: 13.44

GC content: 37.57

Gene sequence:

>1299_bases
ATGGTAGAAAAAAGACAATACAAATCATCCACATTAGGTGTACGTGCAGGTCAAGAACCGGATCCGGTTACTGGAGCATG
TGCTGTACCGATTTATCAAACAAGCTCATATGTATTTAAGGACGCTGCAGAAGCAGCAAGGAGGTTCGGATTACAGGAAT
TTGGTCAAATTTATTCCAGATTAACTAATCCAACAACTGAAGTATTTGAAAAGAGAATAGCTGCTATTGAAGGAGGTAAT
TCTGGATTAGGTACTTCAAGCGGTCTTGCAGCAATTACTTATGCAATTTTAAACATTACATCTCCAGGTGATGAAATCGT
ATCTGCAGATAATCTTTACGGCGGAACTTACCAGCTGTTTGATTACACCTTTAAAGATTTGGCAAGAAATGTTAAATTTG
TGGATTCAAGTGATTTGCAGGCATTTGAAGATGCAATTACTGATAAAACCAAAGCAATTTACTGTGAATCTATAGGCAAT
CCGAAACTGGATGTGCCTGATTTTGAAGCTTTAGCTGAAATTGCTCACAGTCATGACATTCCGTTAATTGTAGATAATAC
AATTGGTGTAGGTTTGGTAAGACCTTTGGAACATGGTGCTGATGTTATTGCAGCATCTGCAACCAAATATGTTGGAGGTC
ATGGAACTTCCATTGGAGGATACATTGTAGATTCAGGTAAATTCAATTGGGGTAATGGTAAATTCCCGCAATTTACAGAA
CCTGATCCAAGTTATCACGGATTGGTATTCTGGGATGCATTTGGTGATGTTCCGGAACTTGGAAATATAGCTTTTACCTT
AAGGGCCAGAGCAAGATTATTACGTGATTTGGGGTCAACTCAGGCACCAACTAACAGTTTCCTGTTTTTACAGGGACTTG
AAAGTTTGGATGTAAGAGTAAAAAGACATTCTGAAAATGCTTTAAAAGTAGCTAAATTCTTGGAATCACATCCTAAAGTT
AAATGGGTAAGTTATCCTGGTTTGGAATCTCACCCTTCACATGAAACTGCTAAAAAATACTTAAACGGTTATTATGCAGG
TATTTTAGGTGTAGGTATTGAAGGCGGTGAGGAAGCAGGTAAGGAATTCATCAATAACTTGGAATTATTCTCTCTTCTTG
CAAATATTGGTGATGCAAAAAGTTTAGCAATACATCCTGCAAGTACTACTCATCAGCAACTTTCAGAAGAAGAACAATTG
GCTACTGGTGTTACACCGGACTTTGTAAGGTTATCTATTGGTCTTGAAGATGCAGATGACTTGATTGATGATCTTTCACG
TGCTTTAGATAAAATTTAA

Upstream 100 bases:

>100_bases
CATCATATTTAGTTTAGAAGGGATAAAAAAAATATGGGATTTTTAAAAATATATGTTATAGTGTAGTAATATTAAATTTA
AAAAAATTACAGGTGATTAT

Downstream 100 bases:

>100_bases
ATTTTAATAAAAAAATAAAAAGGAGATTATAAGATGTATTTGGATAATTCAGCAACTACTGCAGTTAGTGAGGAAGTACT
AAACGAAATGGAGCCTTATT

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVEKRQYKSSTLGVRAGQEPDPVTGACAVPIYQTSSYVFKDAAEAARRFGLQEFGQIYSRLTNPTTEVFEKRIAAIEGGN
SGLGTSSGLAAITYAILNITSPGDEIVSADNLYGGTYQLFDYTFKDLARNVKFVDSSDLQAFEDAITDKTKAIYCESIGN
PKLDVPDFEALAEIAHSHDIPLIVDNTIGVGLVRPLEHGADVIAASATKYVGGHGTSIGGYIVDSGKFNWGNGKFPQFTE
PDPSYHGLVFWDAFGDVPELGNIAFTLRARARLLRDLGSTQAPTNSFLFLQGLESLDVRVKRHSENALKVAKFLESHPKV
KWVSYPGLESHPSHETAKKYLNGYYAGILGVGIEGGEEAGKEFINNLELFSLLANIGDAKSLAIHPASTTHQQLSEEEQL
ATGVTPDFVRLSIGLEDADDLIDDLSRALDKI

Sequences:

>Translated_432_residues
MVEKRQYKSSTLGVRAGQEPDPVTGACAVPIYQTSSYVFKDAAEAARRFGLQEFGQIYSRLTNPTTEVFEKRIAAIEGGN
SGLGTSSGLAAITYAILNITSPGDEIVSADNLYGGTYQLFDYTFKDLARNVKFVDSSDLQAFEDAITDKTKAIYCESIGN
PKLDVPDFEALAEIAHSHDIPLIVDNTIGVGLVRPLEHGADVIAASATKYVGGHGTSIGGYIVDSGKFNWGNGKFPQFTE
PDPSYHGLVFWDAFGDVPELGNIAFTLRARARLLRDLGSTQAPTNSFLFLQGLESLDVRVKRHSENALKVAKFLESHPKV
KWVSYPGLESHPSHETAKKYLNGYYAGILGVGIEGGEEAGKEFINNLELFSLLANIGDAKSLAIHPASTTHQQLSEEEQL
ATGVTPDFVRLSIGLEDADDLIDDLSRALDKI
>Mature_432_residues
MVEKRQYKSSTLGVRAGQEPDPVTGACAVPIYQTSSYVFKDAAEAARRFGLQEFGQIYSRLTNPTTEVFEKRIAAIEGGN
SGLGTSSGLAAITYAILNITSPGDEIVSADNLYGGTYQLFDYTFKDLARNVKFVDSSDLQAFEDAITDKTKAIYCESIGN
PKLDVPDFEALAEIAHSHDIPLIVDNTIGVGLVRPLEHGADVIAASATKYVGGHGTSIGGYIVDSGKFNWGNGKFPQFTE
PDPSYHGLVFWDAFGDVPELGNIAFTLRARARLLRDLGSTQAPTNSFLFLQGLESLDVRVKRHSENALKVAKFLESHPKV
KWVSYPGLESHPSHETAKKYLNGYYAGILGVGIEGGEEAGKEFINNLELFSLLANIGDAKSLAIHPASTTHQQLSEEEQL
ATGVTPDFVRLSIGLEDADDLIDDLSRALDKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=408, Percent_Identity=34.0686274509804, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI25453487, Length=406, Percent_Identity=30.0492610837438, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI299473759, Length=408, Percent_Identity=30.6372549019608, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1790375, Length=428, Percent_Identity=31.0747663551402, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=26.0969976905312, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=432, Percent_Identity=33.7962962962963, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=410, Percent_Identity=32.6829268292683, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=410, Percent_Identity=32.6829268292683, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=48.4018264840183, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319307, Length=439, Percent_Identity=31.4350797266515, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=26.5168539325843, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=320, Percent_Identity=25.625, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=35.6132075471698, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46815; Mature: 46815

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00589 PTS_HPR_SER ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKRQYKSSTLGVRAGQEPDPVTGACAVPIYQTSSYVFKDAAEAARRFGLQEFGQIYSR
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHH
LTNPTTEVFEKRIAAIEGGNSGLGTSSGLAAITYAILNITSPGDEIVSADNLYGGTYQLF
HCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHEECCCCCCCCHHHH
DYTFKDLARNVKFVDSSDLQAFEDAITDKTKAIYCESIGNPKLDVPDFEALAEIAHSHDI
HHHHHHHHCCCEECCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCC
PLIVDNTIGVGLVRPLEHGADVIAASATKYVGGHGTSIGGYIVDSGKFNWGNGKFPQFTE
CEEEECCCCCCCHHHHHCCCCEEHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCC
PDPSYHGLVFWDAFGDVPELGNIAFTLRARARLLRDLGSTQAPTNSFLFLQGLESLDVRV
CCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHH
KRHSENALKVAKFLESHPKVKWVSYPGLESHPSHETAKKYLNGYYAGILGVGIEGGEEAG
HHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHH
KEFINNLELFSLLANIGDAKSLAIHPASTTHQQLSEEEQLATGVTPDFVRLSIGLEDADD
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHH
LIDDLSRALDKI
HHHHHHHHHHCC
>Mature Secondary Structure
MVEKRQYKSSTLGVRAGQEPDPVTGACAVPIYQTSSYVFKDAAEAARRFGLQEFGQIYSR
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHH
LTNPTTEVFEKRIAAIEGGNSGLGTSSGLAAITYAILNITSPGDEIVSADNLYGGTYQLF
HCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHEECCCCCCCCHHHH
DYTFKDLARNVKFVDSSDLQAFEDAITDKTKAIYCESIGNPKLDVPDFEALAEIAHSHDI
HHHHHHHHCCCEECCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCC
PLIVDNTIGVGLVRPLEHGADVIAASATKYVGGHGTSIGGYIVDSGKFNWGNGKFPQFTE
CEEEECCCCCCCHHHHHCCCCEEHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCC
PDPSYHGLVFWDAFGDVPELGNIAFTLRARARLLRDLGSTQAPTNSFLFLQGLESLDVRV
CCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHH
KRHSENALKVAKFLESHPKVKWVSYPGLESHPSHETAKKYLNGYYAGILGVGIEGGEEAG
HHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHH
KEFINNLELFSLLANIGDAKSLAIHPASTTHQQLSEEEQLATGVTPDFVRLSIGLEDADD
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHH
LIDDLSRALDKI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]